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| 1 | The anti-viral protein of trichosanthin penetrates into human immunodeficiency virus type 1显示文摘Trichosanthin (TCS ) 是一种类型我有有势力的使核糖体失去活性的蛋白质对人的免疫不全病毒类型 1 的禁止的活动,并且临床上在获得的免疫不全被使用了症候群(爱滋病) 治疗。以前的研究表明 TCS 认出了人的免疫不全病毒类型 1 (HIV-1 ) 粒子。这里,我们调查了在 TCS 和 HIV-1 粒子之间的物理关系,并且证明 TCS 渗透进病毒的粒子,在它被保护免受各种各样的朊酶消化的伤害的地方。TCS 的穿入不在病毒的正直上施加明显的效果。FYY140142, D176,和 K177 为 membrane-translocation 过程作为关键氨基酸残余被识别。而且,渗透进 HIV-1 virions 的 TCS 显示出有势力抗病毒的活动。总的来说,观察建议进 HIV-1 粒子的 TCS 的穿入可能为消除病毒是重要的。 | Wenlong Zhao Du Feng Shan Sun Ting Han Senfang Sui | 2010 | Acta Biochimica et Biophysica Sinica2010，42，2： | 6 |
| 2 | Effects of genistein, daidzein and glycitein on the osteogenic and adipogenic differentiation of bone marrow stromal cells and on the adipogenic trans-differentiation of osteoblasts显示文摘The 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyl tetrazolium bromide (MTT), alkaline phosphatase (ALP) activity and oil red O assays were used to examine the effects of genistein, daidzein and glycitein on the osteogenic and adipogenic differentiation of primary mouse bone marrow stromal cells (MSCs) and the adipogenic trans-differentiation of primary mouse osteoblasts. The results indicated that daidzein, genistein and glycitein at concentrations from 1×10 -8 mol/L to 1×10 -5 mol/L promoted the proliferation of MSCs and osteoblasts; genistein, daidzein and glycitein promoted osteogenic differentiation and inhibited adipogenic differentiation of MSCs, and inhibited adipocytic transdifferentiation of osteoblasts at appropriate concentrations as 17β-estradiol. It suggests that genistein, daidzein and glycitein regulate a dual differentiational process of MSCs into the osteogenic and adipogenic lineages, and trans-differentiational process of primary osteoblasts into the adipocyte lineages, causing a lineage shift toward osteoblast. Protective effects of them on bone may be mediated by a reversal of adipogenesis which may promote the proliferation, differentiation and mineralization of osteoblasts, and make adipocytes secrete less cytokines which may promote osteoclast formation and activation. In addition, the results also indicated that genistein, daidzein and glycitein may be helpful in preventing the development of steroid induced osteonecrosis. | LI Xianghui ZHANG Jinchao SUI Senfang YANG Mengsu | 2005 | Progress in Natural Science:Materials International2005，15，11： | 5 |
| 3 | Characterization of surface plasmon resonance analysis显示文摘 | Xiao Caide Sui Senfang | 2000 | Sensors and Actuators B:Chemical2000，，66： | 1 |
| 4 | Numerical simulations of surface plasmon resonance system for monitoring DNA hybridization and detecting protein-lipid film interactions 显示文摘 | Xiao Caide Sui Senfang | 1999 | European Biophysics Journal with Biophysics letters1999，2，28： | 1 |
| 5 | Naked-Eye Sensitive Detection of Im- munoglubulin G by Enlargement of Au Nanoparticles In Vitro 显示文摘 | Ma Zhanfang Sui Senfang | 2002 | Angew Chem Int Edit2002，41，12： | 1 |
| 6 | C2 domain of synaptotagmin I associates with lipid rafts of plasma membrane显示文摘In this paper we report that the C2 domain of synaptotagmin I (syt I) could associate with lipid rafts of plasma membrane. We demonstrate that phosphatidylinositol 4,5-bisphosphate (PIP2) in the target membrane and Ca2+ are the key factors to enhance the raft association of the C2 domain. We also found that the raft association of the C2 domain could be fulfilled by either C2A or C2B alone, suggesting that their raft association might be complementary. Finally, we indicate that destroying lipid rafts or blocking syt I-raft association could significantly reduce the Ca2+-driven release of glutamates. Our data indicate that the raft association of the C2 domain might play an important role in the regulated exocytosis. | LU JiHua HE Li SUI SenFang | 2008 | Chinese Science Bulletin2008，53，9： | 1 |
| 7 | Characterization of surface plasmon reso-nance analysis 显示文摘 | Xiao Caide Sui Senfang | 2000 | Sensors and Actuators B : Chemical2000，，66： | 1 |
| 8 | Two forms of the membrane-bound state of the first C2 domain (C2A) of synaptotagminⅠand calcium-triggered membrane insertion显示文摘The synaptic vesicle protein synaptotagminⅠ (sytⅠ) is a vesicle transmembrane protein present in synap-tic vesicles, which has been proposed as the Ca2+ sensor that regulates secretion. The C2A domain is the membrane proximal part of its cytoplasmic domain. The interaction between C2A and lipid bilayer has been considered to be essential for triggering neurotransmitter release. In the pre-sent work, the measurements of membrane surface tension and surface concentration showed that the C2A domain of sytⅠexhibited two membrane-bound states: the surface adsorption state and the membrane insertion state. The sur-face absorption state formed in a Ca2+-independent manner with lower affinity, while the membrane insertion state formed with high affinity was only found in the presence of Ca2+. Both the Ca2+-independent and Ca2+-dependent sytⅠ- membrane interactions required anionic phospholipids, such as phosphatidylserine (PS). When expressed into rat pheo-chromocytoma (PC12) cells and human embryonic kidney (HEK-293) cells, as demonstrated by immunofluorescence staining and subcellular fractionation, most of the C2A was found at the plasma membrane, even when the cells were depleted of Ca2+ by incubation with EGTA. These results suggested a new molecular mechanism of sytⅠas a Ca2+ sensor in membrane fusion. Ca2+-independent surface ad-sorption might attach sytⅠto the release site during the docking or priming step. When intracellular Ca2+ increased, sytⅠtriggered the neurotransmitter release following the Ca2+-dependent penetration into the target membrane. | HE Yuhong, LI Xianghui, WANG Fu, XUE Yi & SUI Senfang Department of Biological Sciences & Biotechnology, State Key Labora-tory of Biomembrane, Tsinghua University, Beijing 100084, China | 2003 | Chinese Science Bulletin2003，48，17： | 0 |
| 9 | Two-dimensional crystallization of photosystem I chlorophyll a/b protein complex from cucumber显示文摘THE photosystem Ⅱ light-harvesting chlorophyll a/b protein complex (LHC Ⅱ) is a kind of integral membrane protein complex in the thylakoid membranes of chloroplasts from higher plants and green algae, and its most important biological function is light harvesting and | LIU Zheng SUI Senfang WANG Shaoxiong LOU Shiqing WANG Kefen ZHAO Fuhong KUANG Tingyun | 1997 | Chinese Science Bulletin1997，42，7： | 0 |
| 10 | Designing the lipid raft marker protein for synaptic vesicles显示文摘Lipid rafts are cholesterol-enriched microdomains and implicated in many essential physiological ac-tivities such as the neurotransmitter release.Many studies have been carried out on the function of rafts inthe plasma membranes,whereas little is known about the information of such microdomains in subcellularcompartments especially synaptic vesicles(SVs).In the well-studied plasma membranes,several proteinshave been recognized as raft markers,which are used to label or trace rafts.But the raft marker proteinon SVs has not been identified yet.Although some SV proteins,including VAMP and CPE,have beenfound in raft fractions,they cannot be used as markers due to their low abundance in rafts.In this work,we designed several chimera proteins and tested their characteristics for using as SV raft makers.First,we detected whether they located in SVs,and then the chimeras exhibiting the better localization in SVswere further examined for their enrichment in raft using detergent treatment and gradient density floatationanalysis.Our results indicate that one of the chimeric proteins is primarily located in SVs and distributedin raft microdomains,which strongly suggests that it could be served as a raft marker for SVs. | 吕继华 Sui Senfang | 2009 | High Technology Letters2009，15，1： | 0 |
| 11 | Regulatory dynamics of the higher-plant PSI-LHCl supercomplex during state transitions显示文摘State transition is a fundamental light acclimation mechanism of photosynthetic organisms in response to the environmental light conditions.This process rebalances the excitation energy between photosystemI(PSl)and photosystem Il through regulated reversible binding of the light-harvesting complex Il(LHCll)to PSl.However,the structural reorganization of PSI-LHCI,the dynamic binding of LHCll,and the regulatory mechanisms underlying state transitions are less understood in higher plants.In this study,using cryoelectron microscopy we resolved the structures of PSI-LHCI in both state 1(PSI-LHCI-ST1)and state 2(PSILHCI-LHCll-ST2)from Arabidopsis thaliana.Combined genetic and functional analyses revealed novel contacts between Lhcb1 and PsaK that further enhanced the binding of the LHCll trimer to the PSI core with the known interactions between phosphorylated Lhcb2 and the PsaL/PsaH/PsaO subunits.Specifically,PsaO was absent in the PSI-LHCI-ST1 supercomplex but present in the PSI-LHCI-LHCIl-ST2 supercomplex,in which the PsaL/PsaK/PsaA subunits undergo several conformational changes to strengthen the binding of PsaO in ST2.Furthermore,the PSI-LHCI module adopts a more compact configuration with shorter Mg-to-Mg distances between the chlorophylls,which may enhance the energy transfer efficiency from the peripheral antenna to the PSl core in ST2.Collectively,our work provides novel structural and functional insights into the mechanisms of light acclimation during state transitions in higher plants. | Jianghao Wu Shuaijiabin Chen Chao Wang Weijun Lin Chao Huang Chengxu Fan Dexian Han Dandan Lu Xiumei Xu SenFang Sui Lixin Zhang | 2023 | Molecular Plant2023，16，12： | 0 |
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