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| 1 | Characterization of cytotoxic compound from marine sediment derived actinomycete Streptomyces avidinii strain SU4显示文摘Objective:To investigate the cytotoxic activity of actinomycete isolated from marine sediment.Methods:In the present study the DNA was isolated and the ITS region of 16s rRNA was amplified by polymerase chain reaction,using two universal bacterial primers,1492K(5'-GGTTACCTTG'TTAC GACTT-3')and Eubac27F(5'-AGAGTTTGATCCTGGCTC AG-3').The amplified products were purified using TIANgel mini purification kit,ligated to MD18-T simple vector(TaKaRa),and transformed into competent cells of Escherichia coli DH5α.16S rRNA gene fragment was sequenced using forward primer M13F(-47)and reverse primer M13R(-48).Blast search sequence similarity was found against the existing non-redundanl nucleotide sequence database thus,identified as Streptomyces sp SU,Streptomyces rubralavandulae strain SU1,Streptomyces cacaoi strain SU2,Streptomyces cavourensis strain SU3,Streptomyces avidinii strain SU4,Streptomyces globisporus strain SU5,Streptomyces variabilis strain SU6,Streptomyces coelicolor strain SU 7.Among the eight identified isolates,one actinomycete Streptomyces avidinii strain SU4 was selected for further study.Results:Crude extract of the actinomycete isolate exhibited IC_(50)in 64.5μg against Hep-2 cell line,250μg in VERO cell line.This value is very close to the criteria of cytotoxicity activity for the crude extracts,as established by the American National Cancer Institute(NCI)is in IC_(50)<30μg/mL.The CC MS analysis showed that the active principle might be 1,2-benzenedicarboxylic acid,bis(2-methylpropyl)ester(12.17%),isooctyl phthalate(15.29%)with the retention time 15.642 and 21.612,respectively.Conclusions:This study clearly proves that the marine sediment derived actinomycetes with bioactive metabolites can be expected to provide high quality biological material for high throughout biochemical and anticancer screening programs.These results help us to conclude that the potential of using metabolic engineering and post genomic approaches to isolate more bioactive compounds and make their possible commercial application is not far off. | Sudha S Masilamani Selvam M | 2012 | Asian Pacific Journal of Tropical Biomedicine2012，2，10： | 2 |
| 2 | Influencc of injection rate of hyperbaric bupivacaine on spinal block in parturients:a randomized trial显示文摘 | Sudha S Morley-Forster PK Shamsah M | | 0，，04： | 1 |
| 3 | Apple pomace as a source of dietary fiber and polyphenols and its effect on the rheological characteristics and cake making 显示文摘 | Sudha M L Baskaran V Leelavathi K | 2007 | Food Chemistry2007，，104： | 1 |
| 4 | Extension of shelf-life of whole-wheat flour by gamma radiation显示文摘 | Marathe S A Machaich J P Rao B Y K Pednekar M D Sudha R V | 2002 | International Journal of Food Science and Technology2002，，37： | 1 |
| 5 | Influence of fiber from different cereals on the rheological characteristics of wheat flour dough and on biscuit quality 显示文摘 | Sudha M L Vetrimani tL Leelavathi K | 2007 | Food Chemistry2007，100，4： | 1 |
| 6 | Interaction of Curculigo- sides and Their ~-Cyclodextrin Complexes with Bo- vine Serum Albumin: A Fluorescence Spectroscopic Study显示文摘 | N Sudha Israel M V Enoch | 2011 | Journal of Solution Chemistry2011，40，10： | 1 |
| 7 | Influence of fiber from different cereals on the rheological characteristics of wheat flour dough and on biscuit quality显示文摘 | SUDHA M L VETRIMANI R LEELAVATHI K | 2007 | Food Chemistry2007，100，： | 1 |
| 8 | Apple pomaee as a source of dietary fiber and polyphenols and its effect on the theological characteristics and cake making 显示文摘 | Sudha M L Baskaran V Leelavathi K | 2007 | Food Chemistry2007，104，： | 1 |
| 9 | Managing white spot disease in shrimp显示文摘 | Wang Y G Shariff M Sudha P M | 1998 | INFOFISH International1998，，3： | 1 |
| 10 | Fat replacement in soft dough biscuits: Its implications on dough rheology and biscuit quality 显示文摘 | Sudha M L Srivastava A K Vetrimani R | 2007 | Journal of Food Engineering2007，80，： | 1 |
| 11 | Factors influencing the careseeking behaviour of chest symptomatics: a community-based study involving rural and urban population in Tamil Nadu, South India 显示文摘 | Sudha G Nirupa C Rajasakthivel M Sivasusbramanian S Sundaram V Bhatt S Subramaniam K Thiruvalluvan E Mathew R Renu G Santhal T | 2003 | Tropical Medicine and International Health2003，8，4： | 1 |
| 12 | Influ-ence of fiber from different cereals on the rheo-logical characteristics of wheat flour dough andon biscuit quality 显示文摘 | Sudha M L Vetrimani R Leelavathi K | 2007 | Food Chemistry2007，100，4： | 1 |
| 13 | Vertical transmission of white spot baculovirus in shrimps-a possibility? 显示文摘 | Mohan C V Sudha P M Shankar K M | 1997 | Current Science1997，73，： | 1 |
| 14 | Influ ence of fiber from different cereals on the rheo logical characteristics of wheat flour dough and on biscuit qualiy显示文摘 | Sudha M L Vetrimani R Leelavathi K | 2007 | Food Chemistry2007，100，： | 1 |
| 15 | Suppression of Pancreatic Cancer by Sulfated Non-Anticoagulant Low Molecular Weight Heparin显示文摘 | Sudha T Yalcin M Lin HY | 2014 | Cancer Lett2014，350，12： | 1 |
| 16 | Influence of defatted soy flour and whey protein concentrate on dough rheologieal charac- teristics and quality of instant vermicelli 显示文摘 | Sudha M L Rajeswari G Rao G V | 2011 | Journal of Texture Studies2011，42，1： | 1 |
| 17 | Factors influencing the careseeking behaviour of chest symptomatics:a community based studin vol ving rural and urban population in Tamil Nadu,South India显示文摘 | Sudha G Nirupa C Rajasakthivel M | | 0，，04： | 1 |
| 18 | Distribution ofcanthaxanthin andlycopene in rats and monkeys显示文摘 | Micheline M M Sudha W Prabahat K S | 1990 | J Nutr1990，120，： | 1 |
| 19 | 显示文摘 | SUDHA M SENTHIKUMAR S HARIHARAN R | 2013 | J Sol-Gel Sci Technol2013，65，： | 1 |
| 20 | Factors influencing the careseeking behaviour of chest symptomatics:a community-based stud involving rural and urban population in Tamil Nadu,South Indi 显示文摘 | Sudha G Nirupa C Rajasakthivel M Sivasusbramanian Sundaram V Bhatt S Subramaniam K Thiruvalluvan EM-athew R Renu G Santhal T | 2003 | TropMed Int Health2003，8，4： | 1 |
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