维普中文期刊产品整合服务
10篇 您的检索式:作者名="STAINES M P"
    题名 作者 年代 出处 被引量
1Calculation of AC loss in an HTS wind turbine generator显示文摘FEE M STAINES M P BUCKLEY R G 2003IEEE Trans on Applied Superconductivity2003,13,2:1
2New antimalarial targets: the example of glucose transport显示文摘Patel A P Staines H M Krishna S 2008Travel Med Infect Dis2008,6,12:1
3The devel- opment of a Robel cable based on 1 MVA HTS transformer 显示文摘STAINES M P GLASSON N D PANNU M 2012Superconductor Science and Technololgy2012,25,01:1
4Ensembl Genomes: an integrative resource for genome-scale data from non- vertebrate species显示文摘Kersey P J Staines D M Lawson D 2012Nucleic Acids Research2012,40,1:1
5Urban-rural variation in mortality and hospital admission rates for unintentional injury in Ireland显示文摘Boland M Staines A Fitzpatrick P 2005Inj Prey2005,11,1:1
6Urban-rural variation in mortality and hospital admission rates for unintentional injury in Ireland显示文摘Boland M Staines A Fitzpatrick P 2005Journal of Epidemiology and Community Health2005,59,7:1
7Prognosis of cerebral vein and dural sinus thrombosis results of the international study cerebral vein and dural sinus thrombosis (ISCVT) 显示文摘Ferro J M Canhao P Stain J 2004Stroke2004,35,3:1
8Gap junctional regulation of signal transduction in bone cells显示文摘Buo A M Stains J P 2014FEBS Lett2014,588,8:1
9Genetic association studies in pre-eclampsia:systematic meta-analyses and field synopsis显示文摘STAINES URIAS E PAEZ M C DOYLE P 0,,06:1
10A cost-effective method to enhance adenoviral transduction of primary murine osteoblasts and bone marrow stromal cells显示文摘We report here a method for the use of poly-L-lysine(PLL) to markedly improve the adenoviral transduction efficiency of primary murine osteoblasts and bone marrow stromal cells(BMSCs) in culture and in situ,which are typically difficult to transduce. We show by fluorescence microscopy and flow cytometry that the addition of PLL to the viral-containing medium significantly increases the number of green fluorescence protein(GFP)-positive osteoblasts and BMSCs transduced with an enhanced GFP-expressing adenovirus. We also demonstrate that PLL can greatly enhance the adenoviral transduction of osteoblasts and osteocytes in situ in ex vivo tibia and calvaria, as well as in long bone fragments. In addition, we validate that PLL can improve routine adenoviral transduction studies by permitting the use of low multiplicities of infection to obtain the desired biologic effect. Ultimately, the use of PLL to facilitate adenoviral gene transfer in osteogenic cells can provide a cost-effective means of performing efficient gene transfer studies in the context of bone research.Atum M Buo Mark S Williams Jaclyn P Kerr Joseph P Stains 2016Bone Research2016,4,2:0
返回顶部 每页显示:
共1页 首页 上一页 第1页 下一页 末页 /1 跳转

网站首页 | 关于我们 | 联系我们 | 产品服务 | 客服中心 | 广告服务 | 版权声明 | 网站联盟 | 友情链接 | 售卡网点

版权所有© 渝B2-20050021-1 渝公网安备 50019002500403号 违法和不良信息举报中心

互联网出版许可证 新出网证(渝)字10号 全国400电话 - 免长途话费