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| 1 | Diagnosis of Helicobacter pylori infection: Current options and developments显示文摘Accurate diagnosis of Helicobacter pylori(H. pylori) infection is a crucial part in the effective management of many gastroduodenal diseases. Several invasive and non-invasive diagnostic tests are available for the detection of H. pylori and each test has its usefulness and limitations in different clinical situations. Although none can be considered as a single gold standard in clinical practice,several techniques have been developed to give the more reliable results. Invasive tests are performed via endoscopic biopsy specimens and these tests include histology,culture,rapid urease test as well as molecular methods. Developments of endoscopic equipment also contribute to the real-time diagnosis of H. pylori during endoscopy. Urea breathingtest and stool antigen test are most widely used noninvasive tests,whereas serology is useful in screening and epidemiological studies. Molecular methods have been used in variable specimens other than gastric mucosa. More than detection of H. pylori infection,several tests are introduced into the evaluation of virulence factors and antibiotic sensitivity of H. pylori,as well as screening precancerous lesions and gastric cancer. The aim of this article is to review the current options and novel developments of diagnostic tests and their applications in different clinical conditions or for specific purposes. | Yao-Kuang Wang Fu-Chen Kuo Chung-Jung Liu Meng-Chieh Wu Hsiang-Yao Shih Sophie SW Wang Jeng-Yih Wu Chao-Hung Kuo Yao-Kang Huang Deng-Chyang Wu | 2015 | World Journal of Gastroenterology2015，21，40： | 66 |
| 2 | 干旱胁迫对苗木蒸腾耗水日变化的影响显示文摘采用BP340 0精密电子天平 ,于 2 0 0 2年夏季研究了在不同水分胁迫条件下 ,4种北方主要造林树种苗木的日蒸腾耗水速率及实际耗水量变化规律 .结果表明 ,在正常水分条件下 ,各苗木的日最大耗水速率出现在 10 :0 0— 14 :0 0之间 ,阔叶树种的蒸腾耗水速率远远大于针叶树种 ,是针叶树种的 5~ 7倍 ,苗木的耗水速率排序为 :黄栌 >火炬树 >侧柏 >油松 ;1年生的黄栌和火炬树与 5年生侧柏的日耗水量相差不大 ,是油松的 1倍 .当苗木受到干旱胁迫后 ,苗木的日最大耗水速率会提前 ,蒸腾速率迅速下降 ,但下降幅度不同 ,耗水速率排序为 :火炬树 >黄栌 >油松 >侧柏 .在中等干旱胁迫下 ,油松、火炬树、侧柏、黄栌的日平均耗水速率分别下降了 5 4 0 %、6 8 6 %、87 2 %和 90 2 % ;侧柏和黄栌之间 ,油松和火炬树之间的日耗水量基本相同 ,但侧柏和黄栌只有油松和火炬树的一半 ;干旱胁迫继续加重后 ,油松、火炬树、侧柏、黄栌的日平均耗水速率只有水分正常条件下的 15 7%、12 1%、4 3%和 9 2 % ,日耗水总量下降到 5 %~10 % ,4个树种间相差不大 . | 招礼军 李吉跃 于界芬 Sophie Bertin | 2003 | 北京林业大学学报2003，25，3： | 59 |
| 3 | 卫生经济学评价报告标准共识2022(CHEERS 2022):卫生经济学评价报告指导意见更新版显示文摘卫生经济学评价是对备选行动方案的成本和结果的比较分析。2013年发表的《卫生经济学评价报告标准共识》(CHEERS)提出了卫生经济学评价的特点,确保结果正确阐释,以支持决策制订。2013版CHEERS旨在指导研究报告撰写者准确报告卫生干预和对照措施、背景、过程、结果等评价细节,使审稿人理解文章内容,帮助读者使用研究结果。本版共识将取代2013版共识,其更加适用于各类卫生经济学评价,结合学科发展和方法更新的需要,更注重患者、公众等相关利益方的参与。本共识适用多种个体或人群健康干预措施(简单或复杂)在不同政策场景的应用分析,包括医疗服务、公共卫生、教育、社会服务等。本文概要介绍了CHEERS 2022共识中包含的28个检查项及每一个检查项的相关建议。CHEERS 2022共识主要用于指导研究人员撰写拟投递同行评议学术期刊的卫生经济学评价文章,亦可用于期刊编辑和审稿专家对待发表文章的评价。熟悉了解本共识要求,还有助于研究人员规划评价研究。随着决策透明度要求提高,本共识可支持卫生技术评估机构建立评价报告标准。 | Don Husereau Michael Drummond Federico Augustovski Esther de Bekker-Grob Andrew H Briggs Chris Carswell Lisa Caulley Nathorn Chaiyakunapruk Dan Greenberg Elizabeth Loder Josephine Mauskopf C Daniel Mullins Stavros Petrou Raoh-Fang Pwu Sophie Staniszewska on behalf of CHEERSISPOR Good Research Practices Task Force 肖月(译) 邱英鹏(译) 史黎炜(译) 张治国(译) 张歆(译) 王海银(译) 翟铁民(译) | 2022 | 英国医学杂志中文版2022，25，8： | 31 |
| 4 | Effectiveness of exercise in hepatic fat mobilization in nonalcoholic fatty liver disease: Systematic review显示文摘AIM: To investigate the efficacy of exercise interventions on hepatic fat mobilization in non-alcoholic fatty liver disease(NAFLD) patients.METHODS: Ovid-Medline, Pub Med, EMBASE and Cochrane database were searched for randomized trials and prospective cohort studies in adults aged ≥ 18 which investigated the effects of at least 8 wk of exercise only or combination with diet on NAFLD from 2010 to 2016. The search terms used to identify articles, in which exercise was clearly described by type, duration, intensity and frequency were: 'NASH', 'NAFLD', 'nonalcoholic steatohepatitis', 'non-alcoholic fatty liver disease', 'fat', 'steatosis', 'diet', 'exercise', 'MR spectroscopy' and 'liver biopsy'. NAFLD diagnosis, as well as the outcome measures, was confirmed by either hydrogen-magnetic resonance spectroscopy(H-MRS) or biopsy. Trials that included dietary interventions along with exercise were accepted if they met all criteria. RESULTS: Eight studies met selection criteria(6 with exercise only, 2 with diet and exercise with a total of 433 adult participants). Training interventions ranged between 8 and 48 wk in duration with a prescribed exercise frequency of 3 to 7 d per week, at intensities between 45% and 75% of VO2 peak. The most commonly used imaging modality was H-MRS and one study utilized biopsy. The effect of intervention on fat mobilization was 30.2% in the exercise only group and 49.8% in diet and exercise group. There was no difference between aerobic and resistance exercise intervention, although only one study compared thetwo interventions. The beneficial effects of exercise on intrahepatic triglyceride(IHTG) were seen even in the absence of significant weight loss. Although combining an exercise program with dietary interventions augmented the reduction in IHTG, as well as improved measures of glucose control and/or insulin sensitivity, exercise only significantly decreased hepatic lipid contents.CONCLUSION: Prescribed exercise in subjects with NAFLD reduces IHTG independent of dietary intervention. Diet and exercise was more effective than exercise alone in reducing IHTG. | Pegah Golabi Cameron T Locklear Patrick Austin Sophie Afdhal Melinda Byrns Lynn Gerber Zobair M Younossi | 2016 | World Journal of Gastroenterology2016，22，27： | 30 |
| 5 | Correlation between in vitro and in vivo immunomodulatory properties of lactic acid bacteria显示文摘AIM: To investigate the correlation between in vitro and in vivo immunomodulation potential of the probiotic strain and its ability to prevent experimental colitis in mice. METHODS: In vitro immunomodulation was assessed by measuring interleukin (IL)-12p70, IL-10, tumor necrosis factor alpha (TNFa) and interferon g (IFNg) release by human peripheral blood mononuclear cells (PBMCs) after 24 h stimulation with 13 live bacterial strains. A murine model of acute TNBS-colitis was next used to evaluate the prophylactic protective capacity of the same set of strains. RESULTS: A strain-specific in vivo protection was observed. The strains displaying an in vitro potential to induce higher levels of the anti-inflammatory cytokine IL-10 and lower levels of the inflammatory cytokine IL-12, offered the best protection in the in vivo colitis model. In contrast, strains leading to a low IL-10/IL-12 cytokine ratio could not significantly attenuate colitis symptoms. CONCLUSION: These results show that we could predict the in vivo protective capacity of the studied lactic acid bacteria (LAB) based on the cytokine profile we established in vitro. The PBMC-based assay we used may thus serve as a useful primary indicator to narrow down the number of candidate strains to be tested inmurine models for their anti-inflammatory potential. | Benoit Foligne Sophie Nutten Corinne Grangette Véronique Dennin Denise Goudercourt Sabine Poiret Joelle Dewulf Dominique Brassart Annick Mercenier Bruno Pot | 2007 | World Journal of Gastroenterology2007，13，2： | 22 |
| 6 | Effect of a fermented milk containing Bifidobacterium lactis DN-173010 on Chinese constipated women显示文摘AIM: To investigate the effect of a fermented milk containing Bifidobacterium lactis DN-173010 and yogurt strains (BIO) on adult women with constipation in Beijing.METHODS: A total of 135 adult females with constipation were randomly allocated to consume for 2 wk either 100 g of the test fermented milk or 100 g of an acidified milk containing non-living bacteria (control).Stool frequency,defecation condition scores,stool consistency and food intake were recorded at baseline and after 1 and 2 wk in an intention-to-treat population of 126 subjects.In parallel,safety evaluation parameters were performed.RESULTS: At baseline,no differences were found between groups.Following consumption of test product,stool frequency was significantly increased after 1 wk (3.5 ± 1.5 vs 2.4 ± 0.6,P < 0.01) and 2 wk (4.1 ± 1.7 vs 2.4 ± 0.6,P < 0.01),vs baseline.Similarly,after 1 and 2 wk,of test product consumption,defecation condition (1.1 ± 0.9 vs 1.9 ± 1.2,P < 0.01 and 0.8 ± 1.0 vs 1.9 ± 1.2,P < 0.01,respectively) and stool consistency (1.0 ± 0.8 vs 1.5 ± 1.1,P < 0.01 and 0.6 ± 0.8 vs 1.5 ± 1.1,P < 0.01,respectively) were significantly improved.Compared with the control group,stool frequency was also significantly increased (3.5 ± 1.5 vs 2.5 ± 0.9,P < 0.01 and 4.1 ± 1.7 vs 2.6 ± 1.0,P < 0.01,respectively),and defecation condition (1.1 ± 0.9 vs 1.6 ± 1.1,P < 0.01 and 0.8 ± 1.0 vs 1.6 ± 1.1,P < 0.01,respectively) and stool consistency (1.0 ± 0.8 vs 1.4 ± 1.0,P < 0.05 and 0.6 ± 0.8 vs 1.3 ± 1.0,P < 0.01,respectively) significantly decreased after 1 and 2 wk of product consumption.During the same period,food intake did not change between the two groups,and safety parameters of the subjects were within normal ranges.CONCLUSION: This study suggests a beneficial effect of a fermented milk containing B.lactis DN-173010 on stool frequency,defecation condition and stool consistency in adult women with constipation constipated women after 1 and 2 wk of consumption. | Yue-Xin Yang Mei He Gang Hu Jie Wei Philippe Pages Xian-Hua Yang Sophie Bourdu-Naturel | 2008 | World Journal of Gastroenterology2008，14，40： | 18 |
| 7 | Robotic surgery for rectal cancer: A systematic review of current practice显示文摘AIM: To give a comprehensive review of current litera-ture on robotic rectal cancer surgery.METHODS: A systematic review of current literature via PubMed and Embase search engines was per-formed to identify relevant articles from january 2007 to november 2013. The keywords used were: 'robotic surgery', 'surgical robotics', 'laparoscopic computer-assisted surgery', 'colectomy' and 'rectal resection'. RESULTS: After the initial screen of 380 articles, 20 pa-pers were selected for review. A total of 1062 patients(male 64.0%) with a mean age of 61.1 years and body mass index of 24.9 kg/m2 were included in the review.Out of 1062 robotic-assisted operations, 831(78.2%) anterior and low anterior resections, 132(12.4%) in-tersphincteric resection with coloanal anastomosis, 98(9.3%) abdominoperineal resections and 1(0.1%) Hart-mann's operation were included in the review. Robotic rectal surgery was associated with longer operative time but with comparable oncological results and anastomotic leak rate when compared with laparoscopic rectal surgery. CONCLUSION: Robotic colorectal surgery has con-tinued to evolve to its current state with promising re-sults; feasible surgical option with low conversion rate and comparable short-term oncological results. The challenges faced with robotic surgery are for more high quality studies to justify its cost. | Tony Wing Chung Mak Janet Fung Yee Lee Kaori Futaba Sophie Sok Fei Hon Dennis Kwok Yu Ngo Simon Siu Man Ng | 2014 | World Journal of Gastrointestinal Oncology2014，6，6： | 16 |
| 8 | 岩石裂隙决定喀斯特关键带地表木本与草本植物覆盖显示文摘地球关键带研究将地表植被组成和功能与岩石层的特性联系起来,为研究喀斯特地区特殊的岩石和土壤条件如何影响地表植被提供了新的思路.文章基于贵州中部喀斯特关键带白云岩和石灰岩分布区岩石、土壤和植被调查结果,发现木本植物覆盖度随宽度1mm以上的裂隙数目增多而线性增加,而草本植物覆盖度则呈相反的趋势(p<0.01).白云岩分布区由于岩性致密、裂隙不发育、土层厚度一般在20cm以内,适合浅根系的草本植物分布.石灰岩分布区由于裂隙发育、土壤分布深、树木的深根系分布在裂隙中,植被组成和结构复杂.基于2001~2010年MODIS遥感数据获取的每16天一个时相的归一化植被指数(NDVI)和年净第一性生产力(NPP)结果进一步表明,石灰岩分布区各时相NDVI总体显著高于白云岩分布区,但多年平均NPP则相反.本文结果表明,在喀斯特关键带,岩性决定土壤的结构和分布,进而决定地上植被木本植物和草本植物的覆盖.尽管石灰岩地区土壤量可能比白云岩地区偏低,但发达的裂隙结构更适合深根系的树木生长,植被活动强.当前喀斯特地区石漠化治理需要充分考虑地球关键带岩石-土壤-植被-大气相互作用,提出适合不同岩性的植被恢复措施. | 刘鸿雁 蒋子涵 戴景钰 吴秀臣 彭建 王红亚 Jeroen MEERSMANS Sophie MGREEN Timothy AQUINE | 2019 | 中国科学:地球科学2019，49，12： | 16 |
| 9 | Systematic review and meta-analysis of colon cleansing preparations in patients with inflammatory bowel disease显示文摘AIM To performed a systematic review and meta-analysis to determine any possible differences in terms of effectiveness, safety and tolerability between existing colon-cleansing products in patients with inflammatory bowel disease.METHODS Systematic searches were performed( January 1980-September 2016) using MEDLINE, EMBASE, Scopus, CENTRAL and ISI Web of knowledge for randomized trials assessing preparations with or without adjuvants, given in split and non-split dosing, and in high(> 3 L) or low-volume(2 L or less) regimens. Bowel cleansing quality was the primary outcome. Secondary outcomes included patient willingness-torepeat the procedure and side effects/complications.RESULTS Out of 439 citations, 4 trials fulfilled our inclusion criteria(n = 449 patients). One trial assessed the impact of adding simethicone to polyethylene glycol(PEG) 4 L with no effect on bowel cleansing quality, but a better tolerance. Another trial compared senna to castor oil, again without any differences in term of bowel cleansing. Two trials compared the efficacy of PEG high-volume vs PEG low-volume associated to an adjuvant in split-dose regimens: PEG low-dose efficacy was not different to PEG high-dose; OR = 0.84(0.37-1.92). A higher proportion of patients were willing to repeat low-volume preparations vs high-volume; OR = 5.11(1.31-20.0). CONCLUSION In inflammatory bowel disease population, PEG lowvolume regimen seems not inferior to PEG high-volume to clean the colon, and yields improved willingness-torepeat. Further additional research is urgently required to compare contemporary products in this population. | Sophie Restellini Omar Kherad Talat Bessissow Charles Ménard Myriam Martel Maryam Taheri Tanjani Peter L Lakatos Alan N Barkun | 2017 | World Journal of Gastroenterology2017，23，32： | 16 |
| 10 | 雅漾舒护活泉水对1050例敏感性皮肤和炎症性皮肤病辅助治疗作用的初步观察显示文摘目的:观察雅漾舒护活泉水对敏感性皮肤和炎症性皮肤病的作用。方法:对1050例敏感性皮肤和炎症性皮肤病急性期的受试者进行观察,在门诊就诊时喷雾1次,5min后观察效果,以后每天喷雾2~3次。455例受试者单独使用雅漾舒护活泉水,595例受试者活泉水与糖皮质激素软膏及抗组胺药联合应用。结果:治疗15d后所有患者的红斑、脱屑、瘙痒等症状均有明显改善,单用雅漾舒护活泉水组和联合用药组疗效差异无显著性(P>0.05)。结论:雅漾舒护活泉水对敏感性皮肤和炎症性皮肤病具有良好的辅助治疗和护理作用。 | 潘炜华 廖万清 温海 刘玮 沈新 Sophie Vitse Therese Nocera | 2005 | 临床皮肤科杂志2005，34，7： | 13 |
| 11 | Shifting equilibriums in Alzheimer’s disease: the complex roles of microglia in neuroinflammation, neuronal survival and neurogenesis显示文摘Alzheimer's disease is the leading cause of dementia.Its increased prevalence in developed countries,due to the sharp rise in ageing populations,presents one of the costliest challenges to modern medicine.In order to find disease-modifying therapies to confront this challenge,a more complete understanding of the pathogenesis of Alzheimer's disease is necessary.Recent studies have revealed increasing evidence for the roles played by microglia,the resident innate immune system cells of the brain.Reflecting the well-established roles of microglia in reacting to pathogens and inflammatory stimuli,there is now a growing literature describing both protective and detrimental effects for individual cytokines and chemokines produced by microglia in Alzheimer's disease.A smaller but increasing number of studies have also addressed the divergent roles played by microglial neurotrophic and neurogenic factors,and how their perturbation may play a key role in the pathogenesis of Alzheimer's disease.Here we review recent findings on the roles played by microglia in neuroinflammation,neuronal survival and neurogenesis in Alzheimer's disease.In each case,landmark studies have provided evidence for the divergent ways in which microglia can either promote neuronal function and survival,or perturb neuronal function,leading to cell death.In many cases,the secreted molecules of microglia can lead to divergent effects depending on the magnitude and context of microglial activation.This suggests that microglial functions must be maintained in a fine equilibrium,in order to support healthy neuronal function,and that the cellular microenvironment in the Alzheimer's disease brain disrupts this fine balance,leading to neurodegeneration.Thus,an understanding of microglial homeostasis,both in health and across the trajectory of the disease state,will improve our understanding of the pathogenic mechanisms underlying Alzheimer's disease,and will hopefully lead to the development of microglial-based therapeutic strategies to restore equilibrium in the Alzheimer's disease brain. | Sophie C. Gray Kerri J. Kinghorn Nathaniel S. Woodling | 2020 | Neural Regeneration Research2020，15，7： | 13 |
| 12 | Dual specimens increase the diagnostic accuracy and reduce the reaction duration of rapid urease test显示文摘AIM:To evaluate the influence of multiple samplings during esophagogastr oduodenoscopy(EGD) on the accuracy of the rapid urease test,and the validity of newly developed rapid urease tests,HelicotecUT plus test and HelicotecUT test,CLO test and ProntoDry test.METHODS:A total of 355 patients undergoing EGD for dyspepsia were included.Their Helicobacter pylori(H.pylori) treatment status was either nave or eradicated.Six biopsy specimens from antrum and gastric body,respectively,were obtained during EGD.Single antral specimens and dual(antrum+body) specimens were compared.Infection status of H.pylori was evaluated by three different tests:culture,histology,and four different commercially available rapid urease tests(RUTs)-including the newly developed HelicotecUT plus test and HelicotecUT test,and established CLO test and ProntoDry test.H.pylori status was defined as positive when the culture was positive or if there were concordant positive results among histology,CLO test and ProntoDry test.RESULTS:When dual specimens were applied,sensitivity was enhanced and RUT reaction time was signif icantly reduced,regardless of their treatment status.Thirty minutes were enough to achieve an agreeable positive rate in all the RUTs.Both newly developed RUTs showed comparable sensitivity,specif icity and accuracy to the established RUTs,regardless of patient treatment status,RUT reaction duration,and EGD biopsy sites.CONCLUSION:Combination of antrum and body biopsy specimens greatly enhances the sensitivity of rapid urease test and reduces the reaction duration to 30 min. | Wen-Hung Hsu Sophie SW Wang Chiao-Yun Chen Ching-Wen Chang Jaw-Yuan Wang Yuan-Chieh Yang Deng-Chyang Wu Ming-Tsang Wu | 2010 | World Journal of Gastroenterology2010，16，23： | 13 |
| 13 | Telerobotic-assisted laparoscopic abdominoperineal resection for low rectal cancer: Report of the first case in Hong Kong and China with an updated literature review显示文摘Telerobotic surgery is the most advanced development in the field of minimally invasive surgery. The da Vinci surgical system, which is currently the most widely used telerobotic device, was approved by the Food and Drug Administration of the United States of America for clinical use in all abdominal operations in July 2000. The first da Vinci surgical system in China was installed in November 2005 at our institution. We herein report the first telerobotic-assisted laparoscopic abdominoperineal resection using the 3-arm da Vinci surgical system for low rectal cancer in Hong Kong and China, which was performed in August 2006. The operative time and blood loss were 240 min and 200 mL, respectively. There was no complication, and the patient was discharged on postoperative day five. An updated review of published literature on telerobotic-assisted colorectal surgery is included in this report, with special emphasis on its advantages and limitations. | Simon Siu-Man Ng Janet Fung-Yee Lee Raymond Ying-Chang Yiu Jimmy Chak-Man Li Sophie Sok-Fei Hon | 2007 | World Journal of Gastroenterology2007，13，17： | 12 |
| 14 | Consequences of bullying victimization in childhood and adolescence:A systematic review and meta-analysis显示文摘AIM To identify health and psychosocial problems associated with bullying victimization and conduct a meta-analysis summarizing the causal evidence.METHODS A systematic review was conducted using Pub Med, EMBASE, ERIC and Psyc INFO electronic databases up to 28 February 2015. The study included published longitudinal and cross-sectional articles that examined health and psychosocial consequences of bullying victimization. All meta-analyses were based on qualityeffects models. Evidence for causality was assessed using Bradford Hill criteria and the grading system developed by the World Cancer Research Fund.RESULTS Out of 317 articles assessed for eligibility, 165 satisfied the predetermined inclusion criteria for meta-analysis.Statistically significant associations were observed between bullying victimization and a wide range of adverse health and psychosocial problems. The evidence was strongest for causal associations between bullying victimization and mental health problems such as depression, anxiety, poor general health and suicidal ideation and behaviours. Probable causal associations existed between bullying victimization and tobacco and illicit drug use. CONCLUSION Strong evidence exists for a causal relationship between bullying victimization, mental health problems and substance use. Evidence also exists for associations between bullying victimization and other adverse health and psychosocial problems, however, there is insufficient evidence to conclude causality. The strong evidence that bullying victimization is causative of mental illness highlights the need for schools to implement effective interventions to address bullying behaviours. | Sophie E Moore Rosana E Norman Shuichi Suetani Hannah J Thomas Peter D Sly James G Scott | 2017 | World Journal of Psychiatry2017，7，1： | 12 |
| 15 | Monetary Policy and Corporate Investment:Evidence from Chinese Micro Data显示文摘This paper investigates how a firm’s characteristics restrict the influence of monetary policy changes on its investment behavior.Focusing on China ’s listed companies for a sample period from the first quarter of 2002 to the first quarter of 2011,we find that quantityoriented and price-based monetary policies have heterogeneous impacts on corporate investment behavior,but the influence of monetary policies is constrained by the liquidity, inventory,size and asset-liability ratio of a firm.Firms with higher liquidity,lower inventory level and lower asset-liability ratios are less sensitive to the impact from two kinds of monetary policies.The larger the size of the firm,the less it is subject to influence from quantity-oriented monetary policy;it responds more to price-based monetary policy.The policy implication is that the monetary authorities should pay attention to the importance of policy-making based on the monetary demand of microeconomic entities. | Ying Sophie Huang Frank M.Song Yizhong Wang | 2012 | China & World Economy2012，20，5： | 12 |
| 16 | Multispecies probiotic protects gut barrier function in experimental models显示文摘AIM:To investigate the effect of the probiotic combination Lactibiane Tolerance?(LT)on epithelial barrier function in vitro and in vivo.METHODS:The effect of the multispecies probiotic LT was assessed on several models of epithelial barrier function both in vitro(in basal and inflammatory conditions)and in vivo[visceral hypersensitivity induced by chronic stress or by colonic perfusion of a fecal supernatant(FSN)from patients with irritable bowel syndrome(IBS)].In vitro,we measured the permeability of confluent T84 cell monolayers incubated with or without LT by evaluating the paracellular flux of macromolecules,in basal conditions and after stimulation with lipopolysaccharide(LPS)or with conditioned medium of colonic biopsies from IBS patients(IBS-CM).In vivo,male C57/Bl6 mice received orally NaCl or LT for 15 d and were submitted to water avoidance stress(WAS)before evaluating visceral sensitivity by measuring the myoelectrical activity of the abdominal muscle and the paracellular permeability with 51Cr-EDTA.Permeability and sensitivity were also measured after colonic instillation of FSN.Tight-junctions were assessed by immunoblotting and TLR-4 expression was evaluated by immunohistochemistry RESULTS:Incubation of T84 cell monolayers with LT in basal conditions had no significant effect on permeability(P>0.05 vs culture medium).By contrast,addition of LT bacterial bodies(LT)completely prevented the LPS-induced increase in paracellular permeability(P<0.01 vs LPS 10 ng/mL(LPS 10);P<0.01 vs LPS 100ng/mL(LPS 100),P>0.05 vs culture medium).The effect was dose dependent as addition of 109 LT bacterial bodies induced a stronger decrease in absorbance than 106 LT(109 LT+LPS 10:-20.1%±13.4,P<0.01vs LPS 10;106 LT+LPS 10:-11.6%±6.2,P<0.01 vs LPS 10;109 LT+LPS 100:-14.4%±5.5,P<0.01 vs LPS 100;106 LT+LPS 100:-11.6%±7.3,P<0.05 vs LPS 100).Moreover,the increase in paracellular permeability induced by culturing T84 cells with conditioned medium of colonic biopsies from IBS patients(IBS-CM)was completely inhibited in the presence of 109 LT(P<0.01 vs IBS-CM).LT also significantly prevented the epithelial disruption induced by intracolonic infusion of fecal supernatant from IBS patients(P<0.01 vs IBS FSN)or water avoidance stress P<0.01 vs WAS)in C57/Bl6 mice and increased the expression of occludin in vitro and in vivo,as assessed by immnunoblotting.The WAS-induced effect on visceral sensitivity was prevented by LT treatment since values obtained for all steps of colorectal distension were significantly(P<0.01)different from the WAS group.Finally,LT downregulated the response mediated through TLR-4 in vitro(decrease in tumor necrosis factorαsecretion in response to LPS:-65.8%for 109 LT and-52.5%for 106LT,P<0.01 vs LPS)and in vivo(inhibition of WAS induced an increase in TLR-4 expression in the LT treated mice colon,P<0.01 vs WAS).CONCLUSION:The probiotic LT mix prevented the disruption to the epithelial barrier induced by LPS,stress or colonic soluble factors from IBS patients and prevented visceral hypersensitivity. | Mylene Nébot-Vivinus Cherryl Harkat Hanene Bzioueche Christel Cartier Raffaella Plichon-Dainese Lara Moussa Helene Eutamene Dorsa Pishvaie Sophie Holowacz Christian Seyrig Thierry Piche Vassilia Theodorou | 2014 | World Journal of Gastroenterology2014，20，22： | 12 |
| 17 | Endovascular management of visceral artery aneurysms: When to watch, when to intervene?显示文摘Visceral artery aneurysms(VAA) include splanchnic and renal artery aneurysms. They represent a rare clinical entity, although their detection is rising due to an increased use of cross-sectional imaging. Rupture is the most devastating complication, and is associated with a high morbidity and mortality. In addition, increased percutaneous endovascular interventions have raised the incidence of iatrogenic visceral artery pseudoaneurysms(VAPAs). For this reason, elective repair is preferable in the appropriately chosen patient. Controversy still exists regarding their treatment. Over the past decade, there has been steady increase in the utilization of minimally invasive, non-operative interventions, for vascular aneurysmal disease. All VAAs and VAPAs can technically be fixed by endovascular techniques but that does not mean they should. These catheter-based techniques constitute an excellent approach in the elective setting. However, in the emergent setting it may carry a higher morbidity and mortality. The decision for intervention has to take into account the size and the natural history of the lesion, the risk of rupture, which is high during pregnancy, and the relative risk of surgical or radiological intervention. For splanchnic artery aneurysms, we should recognize that we are not, in reality, well informed about their natural history. For most asymptomatic aneurysms, expectant treatment is acceptable. For large, symptomatic or aneurysms with a high risk of rupture, endovascular treatment has become the firstline therapy. Treatment of VAPAs is always mandatory because of the high risk of rupture. We present our point of view on interventional radiology in the splanchnic arteries, focusing on what has been achieved and the remaining challenges. | Romaric Loffroy Sylvain Favelier Pierre Pottecher Pierre-Yves Genson Louis Estivalet Sophie Gehin Jean-Pierre Cercueil Denis Krausé | 2015 | World Journal of Radiology2015，7，7： | 11 |
| 18 | Relationship between chromatin organization, mRNAs profile and human male gamete quality显示文摘精子形式是导致把父亲的染色体变成卵母细胞的高度稳定的染色质压缩描绘的能动精子的形成的一个复杂过程。这些 haploid 房间缺乏 transcriptional 和翻译活动并且抄本代表存储 mRNAs 的残余,这通常被保持。最近,成熟精子的染色质组织作为拥有对核酸酶活动敏感的压缩更少的区域的双 nucleoprotamine-nucleohistone 结构被重游,它能在涉及早胚胎开发的基因的表达式被含有。在人的精子的 mRNAs 的一张复杂人口的存在是记录得好的,但是他们的角色还没被阐明。为在从肥沃的人的成熟精子的一个潜伏的 transcriptional 能力或一个潜在的 de novo 翻译的证据为理解精子成熟的最后步是必要的,例如 capacitation 和 acrosome 反应。因此,我们由证明为蛋白质编码的抄本的分叉的层次在也包含了原子冷凝作用记录了在精子质量和精子 RNA 的分发之间的关系(鱼精朊 1 和 2 )或在 capacitation ( eNOS 和 nNOS , c-myc )或在活动性和精子,在低、高的能动精子之间的幸存( aromatase )从一样的样品发出了。因此,分析 mRNAs 的侧面也能作为为在精子发生以后评估男富饶或为为授精的预后使用的一个诊断工具有用。 | Isabelle Galeraud-Denis Sophie Lambard Serge Carreau | 2007 | Asian Journal of Andrology2007，9，5： | 11 |
| 19 | Metastasized pancreatic carcinoma with neoadjuvant FOLFIRINOX therapy and R0 resection显示文摘Patients with metastasized carcinoma of the pancreas have a very poor prognosis, and long-term survival cannot be expected. This case report describes two patients with an initial diagnosis of metastatic pancreatic cancer, both with hepatic metastases and one with an additional peritoneal carcinomatosis. Initially, both patients were treated intravenously with the FOLFIRINOX chemotherapy regimen, consisting of 5-FU, folinic acid, irinotecan and oxaliplatin. Surprisingly, the FOLFIRINOX treatment resulted in complete resolution of the hepatic metastases in both patients, with no lesions detectable by computed tomography scan. Furthermore, treatment response included decreased diameter of the primary tumor in the tail of the pancreas and disappearance of the additional peritoneal carcinomatosis. Both patients were discussed by our multidisciplinary tumor board, which recommended surgical resections of the carcinoma. The R0 resection of the primary tumor was successful in both cases and, interestingly, the resected tissues showed no evidence of the hepatic metastases intraoperatively. In the first case, the patient received a postoperative 6-mo course of adjuvant chemotherapy with gemcitabine. In the second case, the patient continued to receive the FOLFIRINOX regimen for an additional 6 mo postoperatively. At 12 mo after the operation, a nonresectable retroperitoneal lymph node metastasis was detected in the first patient, whereas the second patient remained in complete remission at the time of this report(5 mo after the adjuvant therapy was discontinued). This case report is the first of its kind to describe two cases of hepatic metastatic pancreatic carcinoma that were resectable following treatment with FOLFIRINOX. Further studies are required to examine the role of FOLFIRINOX as a neoadjuvant treatment option in subgroups of patients with initially metastasized pancreatic carcinoma. | Sophie Schneitler Patric Krpil Jasmin Riemer Gerald Antoch Wolfram Trudo Knoefel Dieter Hussinger Dirk Graf | 2015 | World Journal of Gastroenterology2015，21，20： | 9 |
| 20 | 西双版纳兰科植物集市贸易特点和保护启示显示文摘西双版纳是我国兰科植物多样性的热点地区之一。本文对该地区6个主要的集贸市场开展了为期1年(每月1次)的兰科植物贸易调查,以了解该地区野生兰科植物采集和贸易的特点及趋势,为兰科植物的有效保护提供依据。本次调查共记录到兰科植物38属107种,87.9%的种类为附生兰,除铁皮石斛(Dendrobium officinale)外,其余均为野生采集。在记录到的106种野生采集的兰科植物中,93.4%为西双版纳原生分布种,约占该地区野生兰科植物总数的1/4,但大多数种类的记录频次都很低,记录频次在10次以上的仅有14种,说明该地区兰科植物的采集和贸易在种类上具有一定的普遍性和随意性。市场上的兰花售卖者大多数都为当地居民,他们将采集和出售兰花作为经济收入的来源和部分补充。本次调查没有发现跨边境兰花的采集和贸易。西双版纳兰科植物的贸易具有明显的传统性、地域性和季节性,4–6月为兰花贸易的高峰期。石斛属(Dendrobium)植物是最主要的贸易对象,共记录到35种,占总数的32.7%,记录频次最多的种类也都是石斛属植物。本地区药用石斛人工集约化栽培的快速发展并没有减少对野生石斛的采集,反而在一定程度上刺激和促进了市场上的野生石斛贸易。通过对兰花贸易特点的分析,我们针对该地区兰科植物的有效保护提出了针对性的建议,包括应尽快制定和颁布地方性的法律法规,维护和传承本地区传统的植物文化,对古茶园和龙山林等进行持续的保护和采用传统方式管理,开展有针对性的公众教育和宣传来提高公众的保护意识,以及大力发展药用石斛的生态栽培,使更多的当地居民参与其中,并获得收益,从而减少对野生资源的采集。 | 宋亚琼 刘芝龙 Sophie Willian 高江云 | 2017 | 生物多样性2017，25，5： | 9 |
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