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| 1 | Comparison of Mg^(2+)- and Ca^(2+)-enhancing anaerobic granulation in an expanded granular sludge-bed reactor显示文摘The mechanisms responsible for the fast granulation of anaerobic sludge caused by Mg2+ and Ca2+ addition was examined in four lab-scale expanded granular sludge bed(EGSB) reactors. Results indicated that both Mg2+ and Ca2+ accelerated the sludge-granulation process and increased the amount of polysaccharides and proteins in the sludge. Energy dispersive x-ray spectrometry(EDX) analysis revealed that, in a mature granule, both Mg2+ and Ca2+ composed as phosphate and calcium was distributed primarily in the periphery of the granule, while magnesium distributed mainly in the interior. The addition of Mg2+ was more favorable for the nuclei formation, whereas the addition of Ca2+ was more favorable for subgranule growth and maintaining the granules' rigid structure. Results showed that the addition of Mg2+ in the nuclei formation stage and Ca2+ in the granule-growth stage accelerated granulation more than adding only one of them in the granulation process. | CAO XiaoLei SHENG YuXing CAO HongBin ZHANG Yi | 2014 | Science China Chemistry2014，57，11： | 5 |
| 2 | Effects of sulfide on sulfate reducing bacteria in response to Cu(II), Hg(II) and Cr(VI) toxicity显示文摘Sulfate reducing bacteria (SRB) is identified as the primary organisms responsible for the treatment of heavy metal wastewater. However, most heavy metals can inhibit the growth of SRB during heavy metal treatment processes. Sulfide is a metabolic product of SRB and it can precipitate or reduce heavy metals. This study focused on the effects of sulfide on SRB resistance to Cu(II), Hg(I) and Cr(VI) toxicity. First, we considered the existence style of various heavy metals with and without sulfide addition by sequential extraction experiments. Second, the particle size distribution was evaluated and the cell structure during the metabolism of a SRB culture, containing different heavy metals, was analyzed by particle size distribution and TEM analyses. Third, the evolution of sulfate under the influence of different concentrations of heavy metals with and without sulfide addition was investigated to evaluate SRB activity. The results indicated that sulfide played an important role in alleviating and even eliminating the toxicity of Cu(II), Hg(II) and Cr(VI). We also discuss the mechanism of sulfide on SRB resistance to Cu(II), Hg(I) and Cr(VI) toxicity. | SHENG YuXing CAO HongBin LI YuPing ZHANG Yi | 2011 | Chinese Science Bulletin2011，56，9： | 3 |
| 3 | Spatial distribution of bacterial community in EGSB reactor treating synthetic sulfate-containing wastewater at low organic loading rate显示文摘Given that the consumption of organic substances entails costly biodesulfurization,the characteristics of the bacterial community in a reactor should be determined to increase the desulfurizing rate under low organic loading condition.In this study,the bacterial community distribution in the expanded granular sludge bed reactor used to treat sulfate-containing wastewater with low organic loading rate was determined by polymerase chain reaction-denaturing gradient gel electrophoresis(PCRDGGE)and 16S rDNA clone library analyses.DGGE results showed that the predominant bacteria were stable and accounted for*90%sulfate removal efficiency.Differences in band positions and intensities indicated that the distribution and abundance of bacteria were affected by their positions in the reactor.Typical bands were identified in the bacterial community comprising Desulfovibrio,Desulfomicrobium,Thiomonas,Acinetobacter,Bacteroidetes,and Chloroflexi.Their functions in the reactor were also discussed.The possible links between the functional and microbial responses were also investigated based on the characteristic and spatial distribution of each bacterium in consortium. | Kai Zhang Xiaolei Cao Yuxing Sheng Hongbin Cao | 2014 | Chinese Science Bulletin2014，59，1： | 2 |
| 4 | Effects of bacterially produced precipitates on the metabolism of sulfate reducing bacteria during the bio-treatment process of copper-containing wastewater显示文摘A large volume of bacterially produced precipitates are generated during the bio-treatment of heavy metal wastewater.The composition of the bacterially produced precipitates and its effects on sulfate reducing bacteria (SRB) in copper-containing waste stream were evaluated in this study.The elemental composition of the microbial precipitate was studied using electrodispersive X-ray spectroscopy (EDX),and it was found that the ratio of S:Cu was 1.12.Combining with the results of copper distribution in the SRB metabolism culture,which was analyzed by the sequential extraction procedure,copper in the precipitates was determined as covellite (CuS).The bacterially produced precipitates caused a decrease of the sulfate reduction rate,and the more precipitates were generated,the lower the sulfate reduction rate was.The particle sizes of bacterially generated covellite were ranging from 0.03 to 2 m by particles size distribution (PSD) analysis,which was smaller than that of the SRB cells.Transmission electron microscopy (TEM) analysis showed that the microbial covellite was deposited on the surface of the cell.The effects of the microbial precipitate on SRB metabolism were found to be weakened by increasing the precipitation time and adding microbial polymeric substances in later experiments.These results provided direct evidence that the SRB activity was inhibited by the bacterially produced covellite,which enveloped the bacterium and thus affected the metabolism of SRB on mass transfer. | SHENG YuXing,CAO HongBin,LI YuPing & ZHANG Yi National Engineering Laboratory for Hydrometallurgical Cleaner Production Technology Key Laboratory of Green Process and Engineering,Institute of Process Engineering,Chinese Academy of Sciences,Beijing 100190,China | 2010 | Science China Chemistry2010，53，12： | 1 |
| 5 | Mechanisms of Cu 2+ migration, recovery and detoxification in Cu 2+-, -containing wastewater treatment process with anaerobic granular sludge显示文摘 | Xiaolei Cao Hongbin Cao Yuxing Sheng Yongbing Xie Kai Zhang Yi Zhang John C. Crittenden | 2014 | Environmental Technology2014，，15： | 1 |
| 6 | Andrographolide protects against atrial fibrillation by alleviating oxidative stress injury and promoting impaired mitochondrial bioenergetics显示文摘Atrial fibrillation(AF)is the most prevalent cardiac arrhythmia seen in clinical settings,which has been associated with substantial rates of mortality and morbidity.However,clinically available drugs have limited efficacy and adverse effects.We aimed to investigate the mechanisms of action of andrographolide(Andr)with respect to AF.We used network pharmacology approaches to investigate the possible therapeutic effect of Andr.To define the role of Andr in AF,HL-1 cells were pro-treated with Andr for 1 h before rapid electronic stimulation(RES)and rabbits were pro-treated for 1 d before rapid atrial pacing(RAP).Apoptosis,myofibril degradation,oxidative stress,and inflammation were determined.RNA sequencing(RNA-seq)was performed to investigate the relevant mechanism.Andr treatment attenuated RAP-induced atrial electrophysiological changes,inflammation,oxidative damage,and apoptosis both in vivo and in vitro.RNA-seq indicated that oxidative phosphorylation played an important role.Transmission electron microscopy and adenosine triphosphate(ATP)content assay respectively validated the morphological and functional changes in mitochondria.The translocation of nuclear factor erythroid 2-related factor 2(Nrf2)to the nucleus and the molecular docking suggested that Andr might exert a therapeutic effect by influencing the Keap1-Nrf2 complex.In conclusions,this study revealed that Andr is a potential preventive therapeutic drug toward AF via activating the translocation of Nrf2 to the nucleus and the upregulation of heme oxygenase-1(HO-1)to promote mitochondrial bioenergetics. | Pengcheng YU Jiaru CAO Huaxin SUN Yingchao GONG Hangying YING Xinyu ZHOU Yuxing WANG Chenyang QI Hang YANG Qingbo LV Ling ZHANG Xia SHENG | 2023 | Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)2023，24，7： | 0 |
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