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	题名	作者	年代	出处	被引量
1	Lack of correlation between Treg quantification assays in inflammatory bowel disease patients显示文摘AIM:To compare the number of regulatory T-cells( Tregs) measured by flow cytometry with those obtained using a real-time quantitative PCR(q PCR) method in patients suffering from inflammatory bowel disease(IBD).METHODS:Tregs percentages obtained by both flow cytometry and q PCR methods in 35 adult IBD patients,18 out of them with Crohn′s disease(CD)and 17 with ulcerative colitis(UC)were compared to each other as well as to scores on two IBD activity questionnaires using the Harvey Bradshaw Index(HBI)for CD patients and the Simple Colitis Clinical Activity Index(SCCAI)for UC patients.The Treg percentages by flow cytometry were defined as CD4+CD25highCD127lowFOXP3+cells in peripheral blood mononuclear cells,whereas the Treg percentages by q PCR method were determined as FOXP3 promoter demethylation in genomic DNA.RESULTS:We found an average of 1.56%±0.78%Tregs by using flow cytometry,compared to 1.07%±0.53%Tregs by using q PCR in adult IBD patients.There were no significant correlations between either the percentages of Tregs measured by flow cytometry or q PCR and the HBI or SCCAI questionnaire scores in CD or UC patients,respectively.In addition,there was no correlation between Treg percentages measured by q PCR and those measured by flow cytometry(r=-0.06,P=0.73;Spearman Rho).These data suggest that,either Treg-related immune function or the clinical scores in these IBD patients did not accurately reflect actual disease activity.Until the cause(s)for these differences are more clearly defined,the resultssuggest caution in interpreting studies of Tregs in various inflammatory disorders.CONCLUSION:The two methods did not produce equivalent measures of the percentage of total Tregs in the IBD patients studied which is consistent with the conclusion that Tregs subtypes are not equally detected by these two assays.	Gunnar Brandhorst Darinka Todorova Petrova Sebastian Weigand Christoph Eberle Nicolas von Ahsen Jessica Schmitz Frank Christian Schultze Dirk Raddatz Michael Karaus Michael Oellerich Philip D Walson	2015	World Journal of Gastroenterology2015，21，11：	2
2	The interrelationship between surface froth characteristics and industrial flotation performance显示文摘	MOOLMAN D W ALDRICH C SCHMITZ G P J	1996	Minerals Engineering1996，9，8：	1
3	To Tag or not to tag? 显示文摘	Thomas M Caudle D M Schmitz Cecilia M	2009	Library Hi Tech2009，3，：	1
4	Design of robust adaptive frequency hopping for wireless medical telemetry systems显示文摘	Park K J Park T R Schmitz C D		0，，02：	1
5	Shape-selective isopropylation of naphthalene over mordenite catalysts: Computational analysis using MOPAC 显示文摘	Ma X Schmitz A D	1999	Applied Catalysis A :General1999，182，1：	1
6	Mediation of Hippocampal Mossy Fiber Long term Potentiation by Presynaptic Ⅰh Channels显示文摘	MELLOR J NICOLL RA SCHMITZ D	2002	Science(SO036-8075)2002，295，5552：	1
7	Susceptibility oflodgepole pine to infestation by mountain pine beetles following partial cutting of stands显示文摘	Amman G D McGregor M D Schmitz R F	1988	Can J For Res1988，18，：	1
8	Liraglutide vs insulin glargine and placebo in combination with metformin andsulfonylurea therapy in type 2 diabetes mellitus (LEAD-5 met+SU): a randomisedcontrolled tfial显示文摘	Russell-Jones D Vaag A Schmitz O	2009	Diabetologia2009，52，10：	1
9	The use of ultrasound and computed tomography for the diagnosis of a squamous cell carcinoma of the oesophago-cardial region of the stomach in a rhesus monkey显示文摘	Schmitz HC Weishaupt D Borel N		0，，01：	1
10	Rapid preparation of fatty acid esters from lipids for gas chromatographic analysis 显示文摘	METCALFE L D SCHMITZ A A PELKA S R	1966	Analytical chemistry1966，，：	1
11	To tag or not to tag显示文摘	Thomas M Caudle D M Schmitz C M	2009	Library HiTech2009，27，3：	1
12	Shape-selective isopropylation of naphthalene: reactivity of 2,6-diisopropylnaphthalene on dealuminated mordenites 显示文摘	Schmitz A D Song C	1996	Catalysis Today1996，31，12：	1
13	Mass production of genetic markers from a limited number of sorted chromosomes 显示文摘	Vaiman D Pailhoux E Schmitz A	1996	Mamm Genome1996，7，：	1
14	Cognitive elements in the social constructionof communication technologies显示文摘	Fulk J Schmitz J Ryu D	1995	Management Communication Quarterly1995，8，3：	1
15	Bilateral endoscopic total extraperitoneal (TEP) inguinal hernia repair does not induce obstructive azoospermia: data of a retrospective and prospective trial显示文摘	Skawran S Weyhe D Schmitz B	2011	World Journal of Surgery2011，35，7：	1
16	Prognostic factors affecting long-term outcomes in patients with resected stageⅢ A pN2 non-small-cen lungcancer: The 5-year follow-up of a phase II study 显示文摘	Betticher D C Schmitz S F H Totsch M	2006	Br J Cancer2006，94，8：	1
17	Effect of partial cutting treatments of lodgepole pine stands on the abundance and behavior of flying mountain pine beetles显示文摘	Schmitz R F McGregor M D Amman G D	1989	Can J For Res1989，19，：	1
18	Patients with active inflammatory bowel disease lack immature periph- eral blood plasmacytoid and myeloid dendritic cells 显示文摘	Baumgart DC Metzke D Schmitz J	2005	Gut2005，54，2：	1
19	An offer you can't refuse?Ethical implications of non-invasive prenatal diagnosis显示文摘	Schmitz D Netzer C Henn W	2009	Nat Rev Genet2009，10，8：	1
20	Shapeselective isopropylation of naphthalene over mordenite catalysts: computational analysis using MOPAC显示文摘	Song Chunshan Ma Xiaoliang Schmitz A D	1999	Applied Catalysis A: General1999，182，：	1

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