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| 1 | 被激光损伤软骨释放的凋亡因子诱导软骨细胞凋亡的研究显示文摘目的探讨组织损伤后凋亡信号怎样促进细胞的死亡进程。方法在激光照射后猪软骨块置培液中,在不同时间(3、6、9、12、24 h)收集的培液,即实验条件培养液(TCM),从未经激光照射的软骨块培液取得的为对照条件培养液(CCM)。软骨块继续培养28 d,用聚焦显微镜评价其受损区的进展。分离的软骨细胞单层放于 TCM、CCM 或正常培养液(NM)培养,阳性对照组单层细胞经紫外线照射10 min 后继续用正常培养液培养,培养24 h 后,收集细胞并用荧光标记物染色,经流式细胞仪分析。结果所有经 TCM 培养的细胞显现半胱氨酸蛋白水解酶(caspase)-3阳性、CMXRos 和一氧化氮信号减少或失去;经 CCM 或 NM 培养的细胞显现 caspase-3阴性、CMXRos 和一氧化氮信号完好;阳性对照组细胞显现 Caspase-3和 CMXRos 均阳性、一氧化氮信号减少或失去,代表处于凋亡早期的细胞。不同 TCM 浓度(100%、50%、25%、12.5%)和时间(0.5、1、3、9、12 h)对细胞作用后,所有细胞均呈现 caspase-3阳性、CMXRos 和一氧化氮信号减少或失去。结论激光照射受损软骨释放凋亡因子,可诱导单层细胞死亡。 | 郑敏 Grogan SP Schaffner T Mainil-Varlet P | 2007 | 中华医学杂志2007，87，21： | 3 |
| 2 | Diurnal variations in hydraulic conductivity and root pressure can be correlated with the expression of putative aquaporlns in the roots of Lotus japonicus显示文摘 | HENZLER T WATERHOUSE R N SMYTH A J CARVAJAL M COOKE D T SCHAFFNER A R STEUDLE E CLARKSOND T | 1999 | Planta1999，210，： | 1 |
| 3 | Early and late outcome of operated and non-operated acute dissection of the descending aorta显示文摘 | Gysi J Schaffner T Mohaesi P | 1997 | Eur J Cardiothorac Surg1997，11，6： | 1 |
| 4 | Effect of hyperlipemic serum and irradiation on wound healing in primary quiescent cultures of vascular cells显示文摘 | Dimitrievich GS schaffner T | 1989 | Exp Mol Pathol1989，52，： | 1 |
| 5 | Predictive microbiology: where are we, and where are we going 显示文摘 | SCHAFFNER D W LABUZA T P | 1997 | Food Technol1997，51，4： | 1 |
| 6 | Endothelialprotein C receptor function in murine and human breastcancer development显示文摘 | Schaffner F Yokota N Carneiro-Lobo T | 2013 | PLoS One2013，8，61： | 1 |
| 7 | Evolution in invasive plants:implications for biological control显示文摘 | Müller-Sch(a)rer H Schaffner U Steinger T | | 0，，： | 1 |
| 8 | Early and late outcome of operated and non-operated acute dissection of the descending aorta显示文摘 | Gysi J Schaffner T Mohacsi P | 1997 | Eur J of Cardo-thoracic Surgery1997，11，6： | 1 |
| 9 | Diet and dental erosion显示文摘 | Lussi A Jaeggi T SchaffnerⅡ | 2002 | Nutrition2002，18，9： | 1 |
| 10 | Early and late outcome of operatedand non-operated acute dissection of the descending aorta 显示文摘 | Gysi J Schaffner T Mohacsi P | 1997 | Euro J Cardiothorao Surg1997，11，61： | 1 |
| 11 | Early and late outcome of operated and non-operated acute dissection of the descending aorta显示文摘 | Gysi J Schaffner T Mohacsi P | 1997 | Eur J Cardiothorac Surg1997，11，6： | 1 |
| 12 | Endocarditis in intravenous drug addicts and HIV infcted patients:possibilities and limitatious of surgical treatment显示文摘 | Carrel T Schaffner A Vogt P | 1993 | J Heart Valve Dis1993，2，2： | 1 |
| 13 | Evaluating the utility of syndromic surveillance algorithms i'or screening to detect potentially clonal hospital infection outbreaks显示文摘 | Carnevale R J Talbot T R Schaffner W | 2011 | J Am Med Inform Assoc2011，18，4： | 1 |
| 14 | Extended preservation of rat liver graft by induction of heme oxygenase-1 ?显示文摘 | REDAELLI C A TIAN Y H SCHAFFNER T | 2002 | Hepatology2002，35，5： | 1 |
| 15 | Sodium thiosulfate prevents vascular calcifications in uremic rats显示文摘 | Pasch A Schaffner T Huynh-Do U | 2008 | Kidney Int2008，74，11： | 1 |
| 16 | Inhibition of tumor growth and angiogenesis by soluble EphB4 显示文摘 | Martiny-Baron G Korff T Schaffner F | 2004 | Neoplasia2004，6，： | 1 |
| 17 | The mechanics of stridulation of the cricket Gryllus campestr/s 显示文摘 | Koch U T Elliott C J H Schaffner K H | 1988 | Journal of Com- parative Physiology A1988，162，： | 1 |
| 18 | The toxicology of iodate:a review of the literature显示文摘 | B urgi H Schaffner T Seiler JP | 2001 | Thyroid2001，11，5： | 1 |
| 19 | The Toxicology of iodate: A Review of the Literature 显示文摘 | Burgi H Schaffner T Seiler JP | 2001 | Thyroid2001，11，5： | 1 |
| 20 | Differential expression of chemokines in normal pancreas and in chronic pancreatitis 显示文摘 | Saurer L Reber P Schaffner T | 2000 | Gastroenterology2000，118，2： | 1 |
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