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| 1 | hsa-miR-29c and hsa-miR-135b differential expression aspotential biomarker of gastric carcinogenesis显示文摘AIM: To investigate the expression profiles of hsa-mi R-29 c and hsa-mi R-135 b in gastric mucosal samples and their values as gastric carcinogenesis biomarkers. METHODS: The expression levels of hsa-mi R-29 c and hsa-mi R-135 b in normal gastric mucosa, non-atrophic chronic gastritis, intestinal metaplasia and intestinaltype gastric adenocarcinoma were analysed using quantitative real-time PCR. The difference between hsa-mi R-29 c and hsa-mi R-135 b expression profiles in the grouped samples was evaluated by ANOVA and Student's t-test tests. The results were adjusted for multiple testing by using Bonferroni's correction. P values ≤ 0.05 were considered statistically significant. To evaluate hsa-mi R-29 c and hsa-mi R-135 b expressions as potential biomarkers of gastric carcinogenesis, we performed a receiver operating characteristic curve analysis and the derived area under the curve, and a Categorical Principal Components Analysis. In silico identification of the genetic targets of hsa-mi R-29 c and hsa-mi R-135 b was performed using different prediction tools, in order to identify possible genes involved in gastric carcinogenesis.RESULTS: The expression levels of hsa-mi R-29 c were higher in normal gastric mucosal samples, and decreased progressively in non-atrophic chronic gastritis samples, intestinal metaplasia samples and intestinal-type gastric adenocarcinoma samples. The expression of hsa-mi R-29 c in the gastric lesions showed that non-atrophic gastritis have an intermediate profile to gastric normal mucosa and intestinal-type gastric adenocarcinoma, and that intestinal metaplasia samples presented an expression pattern similar to that in intestinal-type gastric adenocarcinoma. This micro RNA(mi RNA) has a good discriminatory accuracy between normal gastric samples and(1) intestinal-type gastric adenocarcinoma; and(2) intestinal metaplasia, and regulates the DMNT3 A oncogene. hsa-mi R-135 b is up-regulated in non-atrophic chronic gastritis and intestinal metaplasia samples and down-regulated in normal gastric mucosa and intestinal-type gastric adenocarcinoma samples. Non-atrophic chronic gastritis and intestinal metaplasia are significantly different from normal gastric mucosa samples. hsa-mi R-135 b expression presented a greater discriminatory accuracy between normal samples and gastric lesions. This mi RNA was associated with Helicobacter pylori presence in non-atrophic chronic gastritis samples and regulates the APC and KLF4 tumour suppressor genes.CONCLUSION: Our results provide evidence of epigenetic alterations in non-atrophic chronic gastritis and intestinal metaplasia and suggest that hsa-mi R-29 c and hsa-mi R-135 b are promising biomarkers of gastric carcinogenesis. | Amanda Ferreira Vidal Aline MP Cruz Leandro Magalhães Adenilson L Pereira Ana KM Anaissi Nélisson CF Alves Paulo JBS Albuquerque Rommel MR Burbano Samia Demachki Ândrea Ribeiro-dos-Santos | 2016 | World Journal of Gastroenterology2016，22，6： | 7 |
| 2 | S-adenosyl-methionine decreases ethanol-induced apoptosis in primary hepatocyte cultures by a c-Jun N-terminal kinase activity-independent mechanism显示文摘AIM:To determine the role of c-Jun N-terminal kinase(JNK)activity in ethanol-induced apoptosis and themodulation of this signaling cascade by S-Adenosyl-methionine(AdoMet).METHODS:Primary hepatocyte cultures werepretreated with 100 μmol/L SP600125,a selective JNKinhibitor,1 mL/L DMSO or 4 mmol/L AdoMet and thenexposed to 100 mmo/L ethanol.Hepatocyte apoptosiswas determined by the TUNEL and DNA ladder assays.JNK activity and its inhibition by SP600125 and AdoMetwere determined by Western blot analysis of c-junphosphorylation and Bid fragmentation.SP600125 andAdoMet effects on the apoptotic signaling pathway weredetermined by Western blot analysis of cytochrome crelease and pro-caspase 3 fragmentation.The AdoMeteffect on glutathione levels was measured by Ellman'smethod and reactive oxygen species(ROS)generationby cell cytometry.RESULTS:The exposure of hepatocytes to ethanolinduced JNK activation,c-jun phosphorylation,Bidfragmentation,cytochrome c release and pro-caspase 3cleavage;these effects were diminished by SP600125,and caused a significant decrease in ethanol-inducedapoptosis(P<0.05).AdoMet exerted an antioxidanteffect maintaining glutathione levels and decreasing ROSgeneration,without a significant effect on JNK activity,and prevented cytochrome c release and pro-caspase 3cleavage. CONCLUSION:The JNK signaling cascade is a keycomponent of the proapoptotic signaling pathwayinduced by ethanol.JNK activation may be independentfrom ROS generation,since AdoMet which exertedantioxidant properties did not have a significant effect onJNK activity.JNK pathway modulator agents and AdoMetmay be components of promising therapies for alcoholicliver disease(ALD)treatment. | Maria del Pilar Cabrales-Romero Lucrecia Márquez-Rosado Samia FatteI-Fazenda Cristina Trejo-Solis Evelia Arce-Popoca Leticia Alemán-Lazarini Saúl Villa-Trevineo | 2006 | World Journal of Gastroenterology2006，12，12： | 2 |
| 3 | Employer retention strategies and their effect on nurses' job satisfaction and intent to stay显示文摘 | Ellenbeeker C H Samia L Cushman M J | 2007 | Home Health Care Serv2007，26，1： | 1 |
| 4 | A middle ear implant,the symphonix vibrant soundbridge:retrospective study of the first 125 patients implanted in france显示文摘 | OLIVIER S DIDIER B SAMIA L | 2003 | Otol Neurotol2003，24，： | 1 |
| 5 | Employer re- tention strategies and their effect on nurses' job satisfaction and intent to stay显示文摘 | Ellenbecker CH Samia L Cushman MJ | 2007 | Home Health Care Serv Q2007，26，1： | 1 |
| 6 | What home heahhcare nurses are saying about their jobs显示文摘 | Ellenbecker CH Boylan LN Samia L | 2006 | Home Heatthc Nurse2006，24，5： | 1 |
| 7 | Further psychometric testing of the home healthcare nurse job satisfaction scale显示文摘 | Ellenbecker C H Byleckie J J Samia L W | 2008 | Res Nurs Health2008，31，2： | 1 |
| 8 | Preparation of an immobilized-stabilized catalase derivative from Aspergillus niger having its multimeric structure stabilized:The effect of Zn2+ on enzyme stability显示文摘 | Samia Kaddour Fernando López-Gallego Tahar Sadoun | 2008 | J Mol Catalysis B:Enzymatic2008，55，4： | 1 |
| 9 | Employer retention strategies and their effect on nurses job satisfaction and intent to stay 显示文摘 | Ellenbeeker CH Samia L Cushman M J | 2007 | Home Health Care Serv Q2007，26，1： | 1 |
| 10 | A middle ear implant, the symphonix vibrant soundbridge: retrospective study of the first 125 patients implanted in france显示文摘 | Olivier S Didier B Samia L | 2003 | Otology & Neurotology2003，24，： | 1 |
| 11 | Employer reten-tion strategies and their effect on nurses job satisfaction andintent to stay 显示文摘 | Ellenbecker CH Samia L Cushman MJ | 2007 | Home Health Care Serv Q2007，26，1： | 1 |
| 12 | Low phospholipid-associated cholelithiasis syndrome:A rare cause of acute pancreatitis that should not be neglected显示文摘BACKGROUND Low phospholipid-associated cholelithiasis(LPAC)syndrome is a very particular form of biliary lithiasis with no excess of cholesterol secretion into bile,but a decrease in phosphatidylcholine secretion,which is responsible for stones forming not only in the gallbladder,but also in the liver.LPAC syndrome may be underreported due to a lack of testing resulting from insufficient awareness among clinicians.AIM To describe the clinical and radiological characteristics of patients with LPAC syndrome to better identify and diagnose the disease.METHODS We prospectively evaluated all patients aged over 18 years old who were consulted or hospitalized in two hospitals in Paris,France(Bichat University Hospital and Croix-Saint-Simon Hospital)between January 1,2017 and August 31,2018.All patients whose profiles led to a clinical suspicion of LPAC syndrome underwent a liver ultrasound examination performed by an experienced radiologist to confirm the diagnosis of LPAC syndrome.Twenty-four patients were selected.Data about the patients’general characteristics,their medical history,their symptoms,and their blood tests results were collected during both their initial hospitalization and follow-up.Cytolysis and cholestasis were expressed compared to the normal values(N)of serum aspartate and alanine transaminase activities,and to the normal value of alkaline phosphatase level,respectively.The subjects were systematically reevaluated and asked about their symptoms 6 mo after inclusion in the study through an in-person medical appointment or phone call.Genetic testing was not performed systematically,but according to the decision of each physician.RESULTS Most patients were young(median age of 37 years),male(58%),and not overweight(median body mass index was 24).Many had a personal history of acute pancreatitis(54%)or cholecystectomy(42%),and a family history of gallstones in first-degree relatives(30%).LPAC syndrome was identified primarily in patients with recurring biliary pain(88%)or after a new episode of acute pancreatitis(38%).When present,cytolysis and cholestasis were not severe(2.8N and 1.7N,respectively)and disappeared quickly.Interestingly,four patients from the same family were diagnosed with LPAC syndrome.At ultrasound examination,the most frequent findings in intrahepatic bile ducts were comet-tail artifacts(96%),microlithiasis(83%),and acoustic shadows(71%).Computed tomography scans and magnetic resonance imaging were performed on 15 and three patients,respectively,but microlithiasis was not detected.Complications of LPAC syndrome required hospitalizing 18 patients(75%)in a conventional care unit for a mean duration of 6.8 d.None of them died.Treatment with ursodeoxycholic acid(UDCA)was effective and well-tolerated in almost all patients(94%)with a rapid onset of action(3.4 wk).Twelve patients’(67%)adherence to UDCA treatment was considered“good.”Five patients(36%)underwent cholecystectomy(three of them were treated both by UDCA and cholecystectomy).Despite UDCA efficacy,biliary pain recurred in five patients(28%),three of whom adhered well to treatment guidelines.CONCLUSION LPAC syndrome is easy to diagnose and treat;therefore,it should no longer be overlooked.To increase its detection rate,all patients who experience recurrent biliary symptoms following an episode of acute pancreatitis should undergo an ultrasound examination performed by a radiologist with knowledge of the disease. | Nicolas Gille Pascale Karila-Cohen Gaël Goujon Dimitrios Konstantinou Samia Rekik Hakim Bécheur Anne-Laure Pelletier | 2020 | World Journal of Hepatology2020，12，6： | 1 |
| 13 | Employer Retention Strategies and their Effect on Nurses Job Satis- faction and Intent to Stay显示文摘 | Ellenbecker C H Samia L Cushman M J | 2007 | Home Health Care Serv Q2007，26，1： | 1 |
| 14 | Employer retention strategies and their effect on nurses' job satisfaction and intent to stay显示文摘 | Ellenbecker CH Samia L Cushman MJ | 2007 | Home Health Care Services Quarterly2007，26，1： | 1 |
| 15 | Employer retentionstrategies and their effect on nurses' job satisfaction and intent tostay 显示文摘 | Ellenbecker CH Samia L Cushman MJ | 2007 | Home Health Care Services Quarterly2007，26，1： | 1 |
| 16 | Caudal regional anesthesia,ropivacaine concentration,postoperative analgesiarand infants显示文摘 | Samia K Hemanth L Manana B | 2006 | Anesth Analg2006，102，： | 1 |
| 17 | Employer retention strategies and their effect on nurses, job satisfaction and intent to stay显示文摘 | Ellenbecker CH Samia L Cushman M J | 2007 | Home Health Care Services Quarterly2007，26，1： | 1 |
| 18 | What home healthcare nurses are saying about their jobs 显示文摘 | Ellenbecker CH Boylan LN Samia L | 2006 | Home Healthc Nurse2006，24，5： | 1 |
| 19 | Enhanced nitrogen doping in TiO2 nanoparticles显示文摘 | Burda C Lou Y Chen X Samia A C Stout J Gole J L | 2003 | Nano letters2003，3，8： | 1 |
| 20 | Employer retention strategies and their effect on nurses' job satisfaction and intent to stay显示文摘 | Ellenbecker CH Samia L Cushman MJ | 2007 | Home Health Care Serv Q2007，26，1： | 1 |
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