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| 1 | HepG2 cells support viral replication and gene expression of hepatitis C virus genotype 4 in vitro显示文摘瞄准:与丙肝的长期的复制建立一个房间文化系统病毒(HCV ) 染色体和病毒的抗原的表示在试管内。方法:HepG2 房间线被孵化与长期的丙肝从一个病人与浆液为它的危险性测试到 HCV。房间和上层清液在文化期间在各种各样的时间点被收获。文化上层清液为它感染天真的房间的能力被测试。存在减(反感觉) 在房间的核心和 E1 抗原的 RNA 海滨,和察觉被 RT-PCR 和免疫学的技术(流动血细胞计数和西方的污点) 分别地检验。结果:细胞内部的 HCV RNA 首先在 d 上被检测 3 在感染以后然后能一致地在至少三个月的一个时期上在房间和上层清液被检测。新鲜房间能从有教养的感染的房间感染上层清液。流动 cytometric 分析证明表面和在房子里使用的细胞内部的 HCV 抗原表示使 polyclonal 成为了抗体(反核心,和 anti-E1 ) 。西方的污点分析证明在分子量的产生免疫性的肽的簇的表示在一个月内在 31 和 45 kDa 之间延长了感染的房间的旧文化而这簇在 uninfected HepG2 房间是无法发现的。结论:HepG2 房间线产生 HCV 感染而且支持它的复制在试管内不仅。HCV 结构的蛋白质的表示能在感染的 HepG2 房间被检测。这些房间也能够流病毒的粒子进接着对 uninfected 房间变得传染的培养基。 | Mostafa K El-Awady Ashraf A Tabll Yasmine S El-Abd Mahmoud M Bahgat Hussein A Shoeb Samar S Youssef Noha G Bader El Din El-Rashdy M Redwan Maha El-Demellawy Moataza H Omran Wael T El-Garf Said A Goueli | 2006 | World Journal of Gastroenterology2006，12，30： | 2 |
| 2 | Effectiveness of hepatitis B virus vaccination program in Egypt:Multicenter national project显示文摘AIM:To assess the effectiveness of hepatitis B virus(HBV) vaccination program among fully vaccinated children.METHODS:A national community based crosssectional study was carried out in 6 governorates representing Egypt. A total of 3600 children aged from 9 mo to 16 years who were fully vaccinated with HBV vaccine during infancy were recruited. Face to face interviews were carried out and sera were evaluated for hepatitis B surface antigen(HBsA g),anti-HBV core antibodies(total) and quantitative detection of hepatitis B surface antibody using enzyme linked immunoassays techniques. Samples positive to HBs Ag/anti-HBV core antibodies were subjected to quantitative HBV-DNA detection by real time polymerase chain reaction with 3.8 IU/L detection limit. RESULTS:Sero-protection was detected among 2059 children(57.2%) with geometric mean titers 75.4 ± 3.6 IU/L compared to 3.1 ± 2.1 IU/L among nonseroprotected children. Multivariate logistic analysis revealed that older age and female gender were the significant predicting variables for having non seroprotective level,with adjusted odds ratio 3.3,9.1and 14.2 among children aged 5 to < 10,10 to < 15 and ≥ 15 years respectively compared to those < 5 years and 1.1 among girls compared to boys with P < 0.01. HBs Ag was positive in 0.11% and breakthrough infection was 0.36% and 0.39% depending on positivity of anti-HBc and DNA detection respectively. The prevalence of HBV infection was significantly higher among children aged ≥ 7 years(0.59%) compared to 0.07% among younger children with odds ratio equal to 8.4(95%CI:1.1-64.2) and P < 0.01.The prevalence was higher among girls(0.48%) than boys(0.29%) with P > 0.05. C ON C LU S I ON :T he E gy pt ian c ompuls or y H B V vaccination program provides adequate protection. Occult HBV infection exists among apparently healthy vaccinated children. Adherence to infection control measures is mandatory. | Iman I Salama Samia M Sami Zeinab Nabil Ahmed Said Manal H El-Sayed Lobna A El Etreby Thanaa M Rabah Dalia M Elmosalami Amany T Abdel Hamid Somaia I Salama Aida M Abdel Mohsen Hanaa M Emam Safaa M Elserougy Amal I Hassanain Naglaa F Abd Alhalim Fatma A Shaaban Samia A Hemeda Nihad A Ibrahim Ammal M Metwally | 2015 | World Journal of Hepatology2015，7，22： | 2 |
| 3 | Is apoptosis present in progresssion to chronic hypertensive heart failure? 显示文摘 | Tamura T Said S Lu W | 2000 | J Card Fail2000，6，1： | 2 |
| 4 | Reverse remodeling of cardiac myocyte hypertrophy in hypertension and failure by targeting of the renin angiotensin system显示文摘 | TAMURA T SAID S HARRIS J | 2000 | Circulation2000，102，2： | 1 |
| 5 | Sensitive measurement of optical nonlinearities using a single beam 显示文摘 | SHEIK-BAHAE M SAID A A WEI T H | 1990 | Journal of Quantum Electronic1990，26，4： | 1 |
| 6 | Activation of PAFreceptor by oxidised LDL in human monocytes releases but not umkinase- type plamimstmactivator expression 显示文摘 | BeaudetLx JL Said T Ninio E | 2004 | Clin Chim Aeta2004，344，2： | 1 |
| 7 | 显示文摘 | BaHae M S Said A A Wei T H | 1990 | IEEE J Quantum Electron1990，26，： | 1 |
| 8 | Vitamin D2 analog 19-nor-1,25-dihydroxyvitamin D2:antitumor activity against leukemia,myeloma,and colon cancer cells显示文摘 | Kumagai T O)'Kelly J Said JW etal | 2003 | Natl Cancer Inst2003，95，12： | 1 |
| 9 | Sensitive measurement of optical nonlinearities using a single beam 显示文摘 | Sheik Bahae M Said A A Wei T | 1990 | IEEE Quantum Electron1990，26，： | 1 |
| 10 | 显示文摘 | Sheik-Bahea M Said A A Wei T H | 1990 | IEEE J Quant Electron1990，26，4： | 1 |
| 11 | ^7Li -nuclear magnetic resonance observation of lithium insertioninto coke carbon modified with mesophase pitch显示文摘 | SAID Y TANUMA KI TAKAYAMA T | 2001 | Journal of Power Sources2001，9797，： | 1 |
| 12 | A Mixed -hybrid Finite Element for Three- dimensional Isotropic Helical Beam Analysis显示文摘 | Mohamed T Fakhreddine D Said A | 2005 | International Journal of Mechanical Sciences2005，47，2： | 1 |
| 13 | Sensitive measurement of optical nonlinearities using a single beam显示文摘 | Mansoor S B Said A A Wei T H et ai | 1990 | IEEE J Quantum Electron1990，26，4： | 1 |
| 14 | The relationship between human spenn apoptosis, morphology and the spenn defonnity index显示文摘 | AZIZ N SAID T PAASCH U | 2007 | Human Reproduction2007，22，5： | 1 |
| 15 | Reverse remodeling of cardiac myocyte hypertrophy in hypertension and failure by targeting of the renin-angiotensin system显示文摘 | TAMURA T SAID S HARRIS J | 2000 | Circulation2000，102，： | 1 |
| 16 | Sensitive measurement of optical nonlinearities using a single beam显示文摘 | Sheik-Bahae M Said A A Wei T H | 1990 | IEEEJ Quant Electron1990，26，4： | 1 |
| 17 | Reverse remodeling of cardiac myocyte hypertrophy in hypertension and failure by targeting of the renin-angiotensin system显示文摘 | amura T Said S Harris J | 2000 | Circulation2000，102，2： | 1 |
| 18 | Seismic response of a cablestayed bridge deck under multi-component non-stationary random ground motion 显示文摘 | Allam Said M Datta T K | 2004 | Earthquake Engineering and Structural Dynamics2004，33，3： | 1 |
| 19 | Early acute rejecion episodes inrenal transplantation in relation to immunosuppres-sion protocols:an audit of 100 ease显示文摘 | NAIR M P NAMPOOHY M R SAID T | 2005 | Transplant Proc2005，37，7： | 1 |
| 20 | Sensitive measurement of optical non-linearities using a single beam显示文摘 | SHEIK B M SAID A A WEI T H | 1990 | IEEE J Quantum Electron1990，26，： | 1 |
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