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您的检索式：作者名="S.M.Mahbubur Rahman"
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| 1 | Antimicrobial activity of kojic acid from endophytic fungus Colletotrichum gloeosporioides isolated from Sonneratia apetala,a mangrove plant of the Sundarbans显示文摘Objective: To isolate and evaluate the antimicrobial activity of the active principle(s) from the ethyl acetate(EtOAc) extract of endophytic fungus Colietotrichum gloeosporioides(C.gloeosporioides) isolated from Sonneratia apetala. Methods: Water agar technique was used to isolate the fungus, and both microscopic and molecular techniques were used for identification of the strain. Potato dextrose broth was used to grow the fungus in large-scale. Reversed-phase preparative HPLC analysis was performed to isolate the major active compound, kojic acid. The EtOAc extract and kojic acid were screened for their antimicrobial activity against two Grampositive and two Gram-negative bacteria as well as a fungal strain using the resazurin 96-well microtitre plate antimicrobial assay. Results: The fungus C. gloeosporioides was isolated from the leaves of Sonneratia apetala. Initial identification of the fugal isolate was carried out using spore characteristics observed under the microscope. Subsequently, the ITS1-5.8 S-ITS2 sequencing was employed for species-level identification of the fungus C. gloeosporioides. Five litres of liquid culture of the fungus produced approximately 610 mg of a mixture of secondary metabolites.Kojic acid(1) was isolated as the main secondary metabolite present in the fungal extract, and the structure was confirmed by 1 D, 2 D NMR and mass spectrometry. The EtOAc extract and compound 1 exhibited considerable antimicrobial activity against all tested microorganisms.Whilst the minimum inhibitory concentration(MIC) values from the EtOAc extract ranged between 2.4×10^(-4)mg/mL and 2.5 mg/mL, those of kojic acid(1) were between 0.125 mg/mL and1 mg/mL. The EtOAc extract and kojic acid(1) were most active against Pseudomonas aeruginosa(MIC = 2.4×10^(-4). mg/mL) and Micrococcus luteus(MIC = 0.125 mg/mL), respectively. Conclusions:The results revealed that the endophytic fungus C. gloeosporioides could be a good source of commercially important kojic acid, which exhibited antimicrobial properties. | Tauhidur Rahman Nurunnabi Shaymaa Al-Majmaie Ismini Nakouti Lutfun Nahar S.M.Mahbubur Rahman Md.Hossain Sohrab Md.Morsaline Billah Fyaz M.D.Ismail George P.Sharples Satyajit D.Sarker | 2018 | Asian Pacific Journal of Tropical Medicine2018，11，5： | 1 |
| 2 | Affine transformation of virtual 3D object using 2D localization of fingertips显示文摘Background Interactions with virtual 3D objects in the virtual reality(VR)environment using the gesture of fingers captured in a wearable 2D camera have emerging applications in real-life.Method This paper presents an approach of a two-stage convolutional neural network,one for the detection of hand and another for the fingertips.One purpose of VR environments is to transform a virtual 3D object with affine parameters by using the gesture of thumb and index fingers.Results To evaluate the performance of the proposed system,one existing,and another developed egocentric fingertip databases are employed so that learning involves large variations that are common in real-life.Experimental results show that the proposed fingertip detection system outperforms the existing systems in terms of the precision of detection.Conclusion The interaction performance of the proposed system in the VR environment is higher than that of the existing systems in terms of estimation error and correlation between the ground truth and estimated affine parameters. | Mohammad Mahmudul ALAM S.M.Mahbubur RAHMAN | 2020 | Virtual Reality & Intelligent Hardware2020，2，6： | 0 |
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