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| 1 | Molecular diagnostics in a teacup:Non-Instrumented Nucleic Acid Amplification(NINA) for rapid,low cost detection of Salmonella enterica显示文摘We report on the use of a novel non-instrumented platform to enable a Loop Mediated isothermal Amplification(LAMP) based assay for Salmonella enterica.Heat energy is provided by addition of a small amount(<150 g) of boiling water,and the reaction temperature is regulated by storing latent energy at the melting temperature of a lipid-based engineered phase change material.Endpoint classification of the reaction is achieved without opening the reaction tube by observing the fluorescence of sequence-specific FRET-based assimilating probes with a simple handheld fluorometer.At or above 22℃ ambient temperature the non-instrumented devices could maintain reactions above a threshold temperature of 61℃ for over 90 min-significantly longer than the 60 min reaction time.Using the simple format,detection limits were less than 20 genome copies for reactions run at ambient temperatures ranging from 8 to 36℃.When used with a pre-enrichment step and non-instrumented DNA extraction device,trace contaminations of Salmonella in milk close to 1 CFU/mL could be reliably detected.These findings illustrate that the non-instrumented amplification approach is a simple,viable,low-cost alternative for field-based food and agricultural diagnostics or clinical applications in developing countries. | KUBOTA Ryo LABARRE Paul WEIGL Bernhard H LI Yong HAYDOCK Paul JENKINS Daniel M | 2013 | Chinese Science Bulletin2013，58，10： | 4 |
| 2 | Mean platelet volume in diabetics显示文摘 | Saigo K Yasunaga M Ryo R | 1992 | Rinsho Byori1992，40，2： | 1 |
| 3 | Adiponectin as a biomarker of the metabolic syndrome显示文摘 | Ryo M | 2004 | Circ2004，68，： | 1 |
| 4 | A robust and precise method for solving the permutation problem of frequency-domain blind source separation显示文摘 | Hiroshi S Ryo M Shoji M | 2004 | IEEE Trans Speech and Audio Processing2004，12，5： | 1 |
| 5 | Activation of an ER-body-localized β-Glucosidase via a cytosolic binding partner in damaged tissues of Arabidopsis thaliana 显示文摘 | Atsushi J N Ryo M Ikuko H N | 2005 | Plant & cell Physiology2005，46，7： | 1 |
| 6 | Role of Pin1 in the regulation of p53 stability and p21 transactivation,and cell cycle checkpoints in response to DNA damage 显示文摘 | WULF G M LIOU Y C RYO A | 2002 | J Biol Chem2002，277，47： | 1 |
| 7 | Computation of stress intensity factors of interface cracks based on interaction energy release rates and BEM sensitivity analysis显示文摘 | TOSHIRO M MASATAKA T RYO O | 2000 | Engi- neering Fracture Mechanics2000，65，： | 1 |
| 8 | Postoperative adjuvant chemotherapy with PVM (Cisplatin + Vindesine + Mitomyein C) and UFT (Uracil + Tegaful)in resected stage Ⅰ - Ⅱ NSCLC ( non-small cell lung cancer ) : a randomized clinical trial 显示文摘 | Hiromi W Ryo M Fumihiro T | 1999 | Eur J Cardio-thoracic Surg1999，15，43： | 1 |
| 9 | Treatment of post-transfusion graft-versus-host disease with nafmostat mesilate,a serine protease inhibitor显示文摘 | Ryo R Saigo K Hashimoto M | 1999 | Vox Sang1999，76，4： | 1 |
| 10 | Expression of adrenomedullin2/intermedin in human brain, heart, and kidney显示文摘 | Ryo M Fumitoshi S Osamu M | 2007 | Peptides2007，，28： | 1 |
| 11 | The distribution and dynamic density of oligodendroglial cytoplastic inclusions (GCIs) in multiple system atrophy:a correlation between the density of GCIs and the degree of involvement of striatonigral and olivopontocerebellar systems显示文摘 | Inoue M Yagishita S Ryo M | 1997 | Acta Neuropathol Berl1997，93，： | 1 |
| 12 | Membrane formation via phase separation induced by penetration of nonsolvent from vapor phase ( Ⅱ ) Membrane morphology显示文摘 | Hideto M Masaaki T Ryo N | 1999 | J Appl Polym Sci1999，74，： | 1 |
| 13 | Glucose-responsive polymer bearing phenylborate derivative as a glucose-sensing moiety operating at the physiological pH显示文摘 | Akira M Ryo Y Kazunori K | 2004 | Biomacromolecules2004，5，： | 1 |
| 14 | Mitochondrial ubiquitin ligase MITOL ubiquitinates mutant SODl and attenuates mutant SODl-induced reactive oxygen species generation显示文摘 | Ryo Y Ayumu S Misako M | 2009 | Mol Bioi Cell2009，20，45： | 1 |
| 15 | Influence of ash composition on heavy metal emission in ash melting process显示文摘 | Ryo Y Nishimura M | 2002 | Fuel2002，81，： | 1 |
| 16 | Control and analysis of the modular multilevelascade converterased on double-starhop-per-cells(MMCC-DSCC)显示文摘 | Makoto H Ryo M Hirofumi A | 2011 | IEEE Trans on Power Electronics2011，26，6： | 1 |
| 17 | Particle - stabilized materi-als :dry oils and ( polymerized) non - aqueous foams显示文摘 | RYO M BISMARCK A | 2010 | Advanced Functional Materials2010，20，5： | 1 |
| 18 | The role of the resin fillet in the delamination of honeycomb sandwich structures显示文摘 | Okada Ryo Kortschot M T | 2002 | Composites Science and Technology2002，62，14： | 1 |
| 19 | The distribution and dynamic density of oligodendroglial cytoplastic inclusions (GCIs) in multiple system atrophy: a correlation between the density of GCIs and the degree of involvement of striatonigral and olivopontocerebellar systems显示文摘 | Inoue M Yagishita S Ryo M | 1997 | Acta Neuropathol Berl1997，93，： | 1 |
| 20 | Influence of ash composition on heavy metal emission in ash melting process显示文摘 | Ryo Y Nishimura M | 2002 | Fuel2002，81，： | 1 |
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