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| 1 | Variation in Plant Morphology and Sinigrin Content in Ethiopian Mustard(Brassica carinata L.)显示文摘Ethiopian mustard(Brassica carinata) is mainly grown as a leafy vegetable and oilseed crop. Sinigrin is predominant glucosinolate in the leaves of Ethiopian mustard. It is hydrolyzed by enzyme myrosinase to produce bioactive product specifically allyl-isothiocyanates. Due to sinigrin content, Ethiopian mustard has a mild flavor and it is a healthy addition to human diet. However, sinigrin concentration depends on genotype.Understanding variations of plant morphology and sinigrin content may be the first step for crop improvement. According to the analyses of various plant characteristics among ninety nine Ethiopian mustard accessions, we found significant differences in plant height, chlorophyll content, and petiole length. In addition, sinigrin content was determined using ultra-performance liquid chromatography tandem quadrupole mass spectrometers/tunable ultraviolet detector(UPLC-TQS/TUV). We found a significant difference in sinigrin content among leaves of 94 Ethiopian mustard accessions. Except for petiole length, the sinigrin content was negatively correlated with leaf area, leaf width, number of primary branches, and plant height. This information will help Ethiopian mustard breeders in screening breeding lines for new accessions. | Tesfay Teklehaymanot Haijing Wang Jianli Liang Jian Wu Runmao Lin Zhen Zhou Xu Cai Xiaowu Wang | 2019 | Horticultural Plant Journal2019，5，5： | 4 |
| 2 | The evolu- tion and pathogenic mechanisms of the rice sheath blight patho- gen 显示文摘 | Zheng Aiping Lin Runmao Zhang Danhua | 2013 | Nature Communications2013，4，： | 1 |
| 3 | Development of InDel Markers for Brassica rapa Based on a High-resolution Melting Curve显示文摘Brassica rapa is one of the most important leafy vegetable crops with large cultivated area in China.To increase the availability of DNA markers in B.rapa,we developed insertion-deletion(InDel)markers utilizing high-resolution melting(HRM)curve analysis.We designed primers for 252 InDels(≥3 bp)evenly distributed in the genome and tested gene polymorphisms with eight accessions.In total,208 markers were specifically amplified,and 148 InDels with polymorphism were genotyped successfully using HRM.We further analyzed the correlation with InDel size,GC number,and predicted the difference in Tm values(Tm)using 208 markers with specific amplification.We found that the success rate of InDel markers was correlated with the GC number of InDel and the predicted-Tm,but not clearly correlated with the length of InDel.When the GC number within InDel was≥8,the successful rate exceeded 90.0%.When the predicted-Tm reached 0.5°C,the success rate was greater than 90.0%,and when it was≥0.6°C,the rate climbed to 100.0%,indicating their role as the optimal parameter for successful development of an applicable InDel marker.The polymorphic InDel markers can be easily genotyped using HRM.They are of great value in genetic analysis,construction of linkage map,and molecular marker-assisted selection in B.rapa. | Rong Chen Lichun Chang Xu Cai JianWu Jianli Liang Runmao Lin Yong Song Xiaowu Wang | 2021 | Horticultural Plant Journal2021，7，1： | 1 |
| 4 | Divergence of three BRX homoeologs in Brassica rapa and its effect on leaf morphology显示文摘The leafy head characteristic is a special phenotype of Chinese cabbage resulting from artificial selection during domestication and breeding.BREVIS RADIX(BRX)has been suggested to control root elongation,shoot growth,and tiller angle in Arabidopsis and rice.In Brassica rapa,three BrBRX homoeologs have been identified,but only BrBRX.1 and BrBRX.2 were found to be under selection in leaf-heading accessions,indicating their functional diversification in leafy head formation.Here,we show that these three BrBRX genes belong to a plant-specific BRX gene family but that they have significantly diverged from other BRX-like members on the basis of different phylogenetic classifications,motif compositions and expression patterns.Moreover,although the expression of these three BrBRX genes differed,compared with BrBRX.3,BrBRX.1,and BrBRX.2 displayed similar expression patterns.Arabidopsis mutant complementation studies showed that only BrBRX.1 could rescue the brx root phenotype,whereas BrBRX.2 and BrBRX.3 could not.However,overexpression of each of the three BrBRX genes in Arabidopsis resulted in similar pleiotropic leaf phenotypes,including epinastic leaf morphology,with an increase in leaf number and leaf petiole length and a reduction in leaf angle.These leaf traits are associated with leafy head formation.Further testing of a SNP(T/C)in BrBRX.2 confirmed that this allele in the heading accessions was strongly associated with the leaf-heading trait of B.rapa.Our results revealed that all three BrBRX genes may be involved in the leaf-heading trait,but they may have functionally diverged on the basis of their differential expression. | Yuanyuan Zhang Jianli Liang Xu Cai Haixu Chen Jian Wu Runmao Lin Feng Cheng Xiaowu Wang | 2021 | Horticulture Research2021，8，1： | 0 |
| 5 | Conserved noncoding sequences correlate with distant gene contacts in Arabidopsis and Brassica显示文摘Physical contact between genes distant on chromosomes is a potentially important way for genes to coordinate their expressions.To investigate the potential importance of distant contacts,we performed high-throughput chromatin conformation capture(Hi-C)experiments on leaf nuclei isolated from Brassica rapa and Brassica oleracea.We then combined our results with published Hi-C data from Arabidopsis thaliana.We found that distant genes come into physical contact and do so preferentially between the proximal promoter of one gene and the downstream region of another gene.Genes with higher numbers of conserved noncoding sequences(CNSs)nearby were more likely to have contact with distant genes.With more CNSs came higher numbers of transcription factor binding sites and more histone modifications associated with the activity.In addition,for the genes we studied,distant contacting genes with CNSs were more likely to be transcriptionally coordinated.These observations suggest that CNSs may enrich active histone modifications and recruit transcription factors,correlating with distant contacts to ensure coordinated expression.This study advances our knowledge of gene contacts and provides insights into the relationship between CNSs and distant gene contacts in plants. | Lei Zhang Jian Wu Jianli Liang Runmao Lin Chao Sun Qirui Dai Lupeng Zhang Huiling Guo Ranze Zhao Xiaowu Wang | 2023 | Journal of Integrative Plant Biology2023，65，6： | 0 |
| 6 | An optimized protocol for detecting guard cell specific gene expression by in situ RT-PCR in Brassica rapa显示文摘It is important to detect specific genes expressed in the guard cells,which control gas exchange and play key roles in response to drought and salt stresses.Due to the genetic transformation of Chinese cabbage(Brassica rapa)has not been well developed,in situ RT-PCR is a valuable option for detecting guard cell specific genes.We reported an optimized protocol of in situ RT-PCR by using an FAMA homologous gene Bra001929 in Brassica rapa.FAMA in Arabidopsis has been verified to be specially expressed in guard cells.We designed specific RT-PCR primers and optimized the protocol in terms of the(a)reverse transcription time,(b)blocking time,(c)antigen-antibody incubation time,and(d)washing temperature.Our approach provides a sensitive and effective in situ RT-PCR method for locating expression in the guard cells in Brassica rapa.Moreover,we proved the guard cell specific expression of Bra001929 in the epidermis indicating its’applicability as a marker gene for guard cells of Brassica rapa. | Yingying Song Xinlei Guo Jian Wu Jianli Liang Runmao Lin Zifu Yan Xiaowu Wang | 2022 | Horticultural Plant Journal2022，8，3： | 0 |
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