|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Relationship of microstructure transformation and hardening behavior of type 17-4 PH stainless steel显示文摘The relationship between the microstructure transformation of type 17-4 PH stainless steel and the aging hardening be- havior was investigated. The results showed that, when 17-4 PH stainless steel aging at 595°C, the bulk hardness of samples attains its peak value (42.5 HRC) for about 20 min, and then decreases at all time. TEM revealed the microstructure corresponding with peak hardness is that the fine spheroid-shape copper with the fcc crystal structure and the fiber-shape secondary carbide M23C6 precipitated from the lath martensite matrix. Both precipitations of copper and M23C6 are the reasons for strengthening of the alloy at this tem- perature. With the extension of holding time at this temperature, the copper and secondary carbide grow and lose the coherent rela- tionship with the matrix, so the bulk hardness of samples decreases. | Jun Wang Hong Zou Cong Li Ruling Zuo Shaoyu Qiu Baoluo Shen | 2006 | Journal of University of Science and Technology Beijing2006，13，3： | 10 |
| 2 | Lyzl4, a novel mouse sperm-related protein, is involved in fertilization显示文摘鸡类型(c 类型) 溶解酵素的角色,原型溶解酵素,在免疫在许多有机体被描绘了。在这研究,我们克隆新奇 c 类型像溶解酵素的基因, Lyzl4,它位于老鼠染色体 9F4 并且与通常认为的信号肽和一个朊酶劈开地点编码了 145 氨基酸。在酵母表示的成熟 recombinant Lyzl4 蛋白质没显示出 bacteriolytic 活动。顺序排列分析证明在 c 类型溶解酵素的 20 不变的残余中的 3 个在 Lyzl4 被改变。改变的氨基酸(D52G ) 之一位于催化领域。Lyzl4 mRNA 有选择地在成年鼠标在睾丸和 epididymis 被表示,与改变越过不同发展阶段的表达式水平。Lyzl4 蛋白质的高水平在 acrosomal 区域和尾巴的主要的片的精子上被发现。在有它的特定的抗体的精子的 Lyzl4 蛋白质的免疫中立化显著地以一种剂量依赖者方式在 vitro 授精百分比减少了,建议 Lyzl4 可能为授精是重要的。 | Ruilin Sun Ruling Shen Jun Li Guojiang Xu Jun Chi Limei Li Jianke Ren Zhugang Wang Jian Fei | 2011 | Acta Biochimica et Biophysica Sinica2011，43，5： | 9 |
| 3 | Combination of onconase and dihydroartemisinin synergistically suppresses growth and angiogenesis of non-small-cell lung carcinoma and mali qnant mesotheliorn~显示文摘 | Ruling Shen Jun Li Danrong Ye Qingcheng Wang Jian Fei | 2016 | Acta Biochimica et Biophysica Sinica2016，48，10： | 6 |
| 4 | STAT3 and AKT signaling pathways mediate oncogenic role of NRSF in hepatocellular carcinoma显示文摘Neuron-restrictive silencer factor(NRSF)is a zinc finger protein that acts as a negative transcriptional regulator by recruiting histone deacetylases and other co-factors.It plays a crucial role in nervous system development and is recently reported to be involved in tumorigenesis in a tumor type-dependent manner;however,the role of NRSF in hepatocellular carcinoma(HCC)tumorigenesis remains unclear.Here,we found that NRSF expression was up-regulated in 27 of 49 human HCC tissue samples examined.Additionally,mice with conditional NRSF-knockout in the liver exhibited a higher tolerance against diethylnitrosamine(DEN)-induced acute liver injury and were less sensitive to DEN-induced HCC initiation.Our results showed that silencing NRSF in HepG2 cells using RNAi technology significantly inhibited HepG2 cell proliferation and severely hindered their migration and invasion potentials.Our results demonstrated that NRSF plays a pivotal role in promoting DEN-induced HCC initiation via a mechanism related to the STAT3 and AKT signaling pathways.Thus,NRSF could be a potential therapeutic target for treating human HCC. | Ming Ma Yunhe Zhou Ruilin Sun Jiahao Shi Yutong Tan Hua Yang Mengjie Zhang Ruling Shen Leon Xu Zhugang Wang Jian Fei | 2020 | Acta Biochimica et Biophysica Sinica2020，52，10： | 1 |
| 5 | Isolation and Identification of a Cellulose-degrading Bac- terium from Paddy Soils in Chengdu City显示文摘In this study,a cellulose-degrading bacterium was isolated from paddy soils in Chengdu City.After 2 d of culture,the average diameter of hydrolytic circles on CMC-Congo red medium was 26-30 mm.Single colonies on LB plates were white and wrinkled.The isolated strain was identified as a Gram-negative bacterium by Gram staining,which could produce short rod-like spores.Colony PCR was performed using the bacterial 16S rDNA universal primers.A 1 429 kb DNA fragment was amplified.Based on sequence homology analysis and comprehensive analysis,the isolated strain was identified as Bacillus velezensis,which was named CDBV62 according to the origin. | Yulong CHEN Zhiyu SUN Jintao LI Ling SHEN Ruling HONG | 2016 | Agricultural Biotechnology2016，5，5： | 1 |
| 6 | Epidermal growth factor receptor is overexpressed in neuroblastoma tissues and cells显示文摘Neuroblastoma 是在童年的最普通的腹的恶意的肿瘤。指向肿瘤房间表面受体的 Immunotoxin (信息技术) 是一条新增补治疗学的处理途径。如果信息技术治疗能被用来对待倔强的 neuroblastoma,这研究的目的是在 neuroblastoma 房间线和纸巾检测表皮的生长因素受体(EGFR ) 的表示,并且探索。在人的 neuroblastoma 织物样品的 EGFR 表示被 immunohistochemistry 检测 ? 染色。EGFR 表示的积极的率在 neuroblastoma 织物是 81.0% 并且 50.0% 在 gangliocytoma,分别地,但是没有在他们之间的统计意义(P ?>? 0.05 ) 。分别地,在有利类型和相反的类型的 EGFR 表示的积极的率是 62.5% 和 92.3% ,但是他们不是统计上不同的(P ?>? 0.05 ) 。从化疗前和化疗以后的样品的结果证明没有重要统计差别(P ?>? 0.05 ) 在他们之间在 EGFR 表示。而且,在五根 neuroblastoma 房间线的 EGFR 表示层次用基于房间的 ELISA 试金和西方的污点分析被测量。结果证明 EGFR 的表示在 KP-N-NS 是更高的并且是(2 )-C 在另外的房间比那些排队。我们的结果表明有在 neuroblastoma 纸巾以及在 neuroblastoma 房间线的 EGFR 的一致、普遍的表情,建议开发由在 EGFR 指向的 neuroblastoma 的未来处理策略是可能的。 | Chao Zheng Ruling Shen Kai Li Na Zheng Yuqing Zong Danrong Ye Qingcheng Wang Zuopeng Wang Lian Chen Yangyang Ma | 2016 | Acta Biochimica et Biophysica Sinica2016，48，8： | 0 |
| 7 | Dnmt3a is required for the tumor stemness of B16 melanoma cells显示文摘The relationship of carcinogenesis and DNA methyltransferases has attracted extensive attention in tumor research. We reported previously that inhibition of de novo DNA methyltransferase 3a (Dnmt3a) in murine B16 melanoma cells significantly suppressed tumor growth and metastasis in xenografted mouse model. Here, we further demonstrated that knockdown of Dnmt3a enhanced the proliferation in anchor-independent conditions of B16 cells, but severely disrupted its multipotent differentiation capacity in vitro. Furthermore, transforming growth factor β1, a key trigger in stem cell differentiation and tumor cell epithelial–mesenchymal transition (EMT), mainly induced apoptosis, but not EMT in Dnmt3a-deficient B16 cells. These data suggested that Dnmt3a is required for maintaining the tumor stemness of B16 cells and it assists B16 cells to escape from death during cell differentiation. Thus it is hypothesized that not only extraordinary self-renewal ability, but also the capacity of multipotent differentiation is necessary for the melanoma tumorigenesis. Inhibition of multipotent differentiation of tumor cells may shed light on the tumor treatment. | Haoyue Wang Ying Kuang Jun Li Ruling Shen Ruilin Sun Danyi Huang Zhejin Sheng Jiahao Shi Mengjie Zhang Fang Huang Hua Yang Jian Fei | 2019 | Acta Biochimica et Biophysica Sinica2019，51，9： | 0 |
| 8 | An EGF receptor-targeting amphinase recombinant protein mediates anti-tumor activity in vitro and in vivo显示文摘利用连接到作为反肿瘤药指向分子的肿瘤的细胞毒素的蛋白质是一条有希望的途径。然而,从细菌或植物导出的大多数细胞毒素有象大分子的重量那样的固有的问题,他们触发强壮的免疫系统反应,它导致药失败和严肃的副作用。Amphinase (Amph ) 是有在北豹青蛙卵母细胞被发现的低分子的重量的 ribonuclease。它在 vivo 在 vitro 和弱 immunogenicity 对肿瘤房间线有强壮的 cytotoxicity,并且是在指向的药的发展的一个有希望的候选人。转变生长因素 --(TGF-) 那绑到表皮的生长因素受体(EGFR ) 为 EGFR 高表示的肿瘤的处理被用作一个指向的分子。在这研究,我们从 Escherichia coli 独立表示了并且净化 recombinant amphinase 和它的 TGF- 熔化蛋白质(AGT ) 。AGT 在 vivo 在 EGFR 高表示的肿瘤房间线,和更强壮的反肿瘤效果上在 vitro 展出了更重要的 cytotoxicity。这熔化蛋白质也展出了不平常的 thermostability,在 vivo immunogenicity,和副作用低。我们的结果为与低 immunogenicity 指向生物代理人的新奇、高度有效的反肿瘤的发展提供一个新入口点。 | Ruling Shen Danrong Ye Qin Huang Jun Li Qingcheng Wang Jian Fei | 2018 | Acta Biochimica et Biophysica Sinica2018，50，4： | 0 |
您所在的机构暂未开通此部分的功能，请联系管理员