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| 1 | m TOR signaling in liver regeneration: Rapamycin combined with growth factor treatment显示文摘AIM: To investigate the effects of mammalian target of rapamycin(mT OR) inhibition on liver regeneration and autophagy in a surgical resection model.METHODS: C57BL/6 mice were subjected to a 70% partial hepatectomy(PH) and treated intraperitoneally every 24 h with a combination of the m TOR inhibitor rapamycin(2.5 mg/kg per day) and the steroid dexamethasone(2.0 mg/kg per day) in phosphate bufferedsaline(PBS) or with PBS alone as vehicle control. In the immunosuppressant group, part of the group was treated subcutaneously 4 h prior to and 24 h after PH with a combination of human recombinant interleukin 6(IL-6; 500 μg/kg per day) and hepatocyte growth factor(HGF; 100 μg/kg per day) in PBS. Animals were sacrificed 2, 3 or 5 d after PH and liver tissue and blood were collected for further analysis. Immunohistochemical staining for 5-Bromo-2'-deoxyuridine(Brd U) was used to quantify hepatocyte proliferation. Western blotting was used to detect hepatic microtubule-associated protein 1 light chain 3(LC3)-Ⅱ protein expression as a marker for autophagy. Hepatic gene expression levels of proliferation-, inflammation- and angiogenesisrelated genes were examined by real-time reverse transcription-polymerase chain reaction and serum bilirubin and transaminase levels were analyzed at the clinical chemical core facility of the Erasmus MC-University Medical Center.RESULTS: m TOR inhibition significantly suppressed regeneration, shown by decreased hepatocyte proliferation(2% vs 12% Brd U positive hepatocyte nuclei at day 2, P < 0.01; 0.8% vs 1.4% at day 5, P = 0.02) and liver weight reconstitution(63% vs 76% of initial total liver weight at day 3, P = 0.04), and furthermore increased serum transaminase levels(aspartate aminotransferase 641 U/L vs 185 U/L at day 2, P = 0.02). Expression of the autophagy marker LC3-Ⅱ, which was reduced during normal liver regeneration, increased after mT OR inhibition(46% increase at day 2, P = 0.04). Hepatic gene expression showed an increased inflammation-related response [tumor necrosis factor(TNF)-α 3.2-fold upregulation at day 2, P = 0.03; IL-1Ra 6.0-fold upregulation at day 2 and 42.3-fold upregulation at day 5, P < 0.01] and a reduced expression of cell cycle progression and angiogenesis-related factors(HGF 40% reduction at day 2; vascular endothelial growth factor receptor 2 50% reduction at days 2 and 5; angiopoietin 1 60% reduction at day 2, all P ≤ 0.01). Treatmentwith the regeneration stimulating cytokine IL-6 and growth factor HGF could overcome the inhibitory effect on liver weight(75% of initial total liver weight at day 3, P = 0.02 vs immunosuppression alone and P = 0.90 vs controls) and partially reversed gene expression changes caused by rapamycin(TNF-α and IL-1Ra levels at day 2 were restored to control levels). However, no significant changes in hepatocyte proliferation, serum injury markers or autophagy were found.CONCLUSION: mT OR inhibition severely impairs liver regeneration and increases autophagy after PH. These effects are partly reversed by stimulation of the IL-6 and HGF pathways. | Suomi MG Fouraschen Petra E de Ruiter Jaap Kwekkeboom Ron WF de Bruin Geert Kazemier Herold J Metselaar Hugo W Tilanus Luc JW van der Laan Jeroen de Jonge | 2013 | World Journal of Transplantation2013，3，3： | 6 |
| 2 | The Need of Industry to Go FAIR显示文摘The industry sector is a very large producer and consumer of data,and many companies traditionally focused on production or manufacturing are now relying on the analysis of large amounts of data to develop new products and services.As many of the data sources needed are distributed and outside the company,FAIR data will have a major impact,both by reducing the existing internal data silos and by enabling the efficient integration with external(public and commercial)data.Many companies are still in the early phases of internal data”FAIRification”,providing opportunities for SMEs and academics to apply and develop their expertise on FAIR data in collaborations and public-private partnerships.For a global Internet of FAIR Data&Services to thrive,also involving industry,professional tools and services are essential.FAIR metrics and certifications on individuals,data,organizations,and software,must ensure that data producers and consumers have independent quality metrics on their data.In this opinion article we reflect on some industry specific challenges of FAIR implementation to be dealt with when choices are made regarding”Industry GOing FAIR”. | Herman van Vlijmen Albert Mons Arne Waalkens Wouter Franke Arie Baak Gerbrand Ruiter Christine Kirkpatrick Luiz Olavo Bonino da Silva Santos Bert Meerman Renger Jellema Derk Arts Martijn Kersloot Sebastiaan Knijnenburg Scott Lusher Rudi Verbeeck Jean-Marc Neefs | 2020 | Data Intelligence2020，2，1： | 4 |
| 3 | Soil biodiversity for agricultural sustainability显示文摘 | Lijbert Brussaard Peter C. de Ruiter George G. Brown | 2006 | Agriculture, Ecosystems and Environment2006，，3： | 3 |
| 4 | Towards an understanding of Type Ia supernovae from a synthesis of theory and observations显示文摘 | W. Hillebrandt M. Kromer F. K. Ropke A. J. Ruiter | 2013 | Frontiers of physics2013，8，2： | 3 |
| 5 | 草甘膦制剂中非离子和阳离子型表面活性剂的影响显示文摘草甘膦制剂中表面活性剂的浓度偏高(0.1%W/V)时可能会增加植物毒性。由于还不完全认识表面活性剂对草甘膦毒性的影响,本研究试图通过两种表面活性剂对冬小麦的影响,以及用萤光法测定表面活性剂与叶面沉积物的关系,用电子显微镜观察不同浓度的表面活性剂对叶片膜质渗透与^(14)C-草甘膦的吸收关系,来解释表面活性剂的作用机理,从而能更合理地指导表面活性剂的使用。 | H.de Ruiter M.A.M.Verbeek 黄群 程隆新 | 1991 | 农药译丛1991，13，6： | 2 |
| 6 | 应用ICF扩大慢性神经性疾病中健康问题范围的研究显示文摘目的确定能够显示慢性神经功能障碍(多发性硬化症、帕金森氏病和神经肌肉疾病)患者有关健康问题的ICF类目。方法采用Delphi研究方法,由三方面与疾病相关的人组成专家小组,即患者及其代理人、医疗和非医疗卫生专业人士(n=98)。小组要求根据《国际功能,残疾和健康分类》(ICF)反映有关特定疾病的健康问题,选择相关类目。小组成员将这些类目与简明残疾记录量表(MRD)和残疾及其影响量表(DIP)两种测量工具中的指标作比较。结果选择出68项ICF类目相关性最大,分别属于四个ICF领域。患者/代理人和卫生专业人员两者评价的类目无显著性差异。疾病小组的一致性很高。三个特定疾病小组的选择在'身体功能和结构'领域存在差异,但如果扩大纳入标准则可以达成共识。所选择的ICF项目涵盖了几乎所有测量项目。最大的差异表现在'参与'和'环境因素'领域选择的类目。结论与广泛应用于神经学领域的健康状态测量相比,在研究健康问题时所选择的ICF类目更为广泛,特别是涉及ICF的'参与'和'环境因素'领域时。 | Klaske WYNIA Berrie MIDDEL Jitse P. van DIJK Han de RUITER Willem LOK Jacques Ha de KEYSER Sijmen A. REIJNEVELD 王朴(译) 张君梅(译) 荀芳(译) 邱卓英(审校) 李建军(审校) | 2008 | 中国康复理论与实践2008，14，12： | 2 |
| 7 | Risk of cancer in patients on insulin glargine and other insulin analogues in comparison with those on human insulin:results from a large population-based follow-up study显示文摘 | Ruiter R Visser LE van Herk-Sukel MP | | Diabetologia0，，： | 2 |
| 8 | Regulated expression of the inhibitory receptor LAIR-1 on human peripheral T cells during T cell activation and differentiation显示文摘 | Jansen CA Cruijsen CW de Ruiter T | 2007 | J Immunol2007，37，4： | 1 |
| 9 | The interplay between gesture and speech in the production of referring expressions:Investigating the tradeoff hypothesis显示文摘 | Ruiter J.P Bangerter A Dings P | | 0，，： | 1 |
| 10 | Color of smoked foods 显示文摘 | Ruiter A | 1979 | Food Technol1979，33，： | 1 |
| 11 | Anatomical basis of autonomic nerve-preserving total nesorectal excision for rectal cancer 显示文摘 | Havenga K De Ruiter MC Enker WE | 1996 | Br J Surg1996，83，4： | 1 |
| 12 | Development of the cardiac conduction tissue in human embryos using HNK-1 antigen expression:Possible relevant for understanding of abnormal atrial automaticity显示文摘 | Gittaenberger-De Groot AC De Ruiter MC | 1999 | Circulation1999，99，6： | 1 |
| 13 | Changes in phototaxis during the larval life of the eyed hawk moth显示文摘 | DE Ruiter VAN DER Horn I J | 1957 | Nature1957，179，： | 1 |
| 14 | Direct control of the Forkhead transcription factor AFX by protein kinase B显示文摘 | Kops G J de Ruiter ND de Vries-Smits AM | 1999 | Nature1999，398，6728： | 1 |
| 15 | Diabetes im-pairs arteriogenesis in the peripheral circulation : review ofmolecular mechanisms 显示文摘 | Ruiter MS van Golde JM Schaper NC | 2010 | Clin Sri (Ixinti)2010，119，6： | 1 |
| 16 | Management of ob- structive sleep apnea in edentulous patients: an overview of the literature显示文摘 | Heidsieck DS de Ruiter MH de Lange J | 2016 | Sleep Breath2016，20，1： | 1 |
| 17 | the role of coping appraisal in reactions to fear appeals:Do we need threat information?显示文摘 | Ruiter R Verplanken B Kok G | 2003 | Journal of Health Psychology2003，8，4： | 1 |
| 18 | Inhibition of hydroxyl-radical-generated DNA degradation by metallothionein显示文摘 | Abel J Ruiter N | 1989 | Toxicol lett1989，47，： | 1 |
| 19 | RNA interference by expression of short interfering RNAs and hairpin RNAs in mammalian cells 显示文摘 | Yu J Y De Ruiter S L Turner D L | 2002 | Proc Natl Acad Sci USA2002，99，9： | 1 |
| 20 | Development and degeneration of retina in rds mutant mice∶ light microscopy显示文摘 | De Ruiter A Hawkins RK | 1980 | J Comp Neurol1980，194，： | 1 |
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