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| 1 | Growth inhibition and apoptosis induction Sulindac on Human gastric cancer cells显示文摘AIM: To evaluate the effects of sulindac in inducing growth inhibition and apoptosis of human gastric cancer cells in comparison with human hepatocellular carcinoma (HCC)cells.METHODS: The human gastric cancer cell lines MKN45 and MKN28 and human hepatocellular carcinoma cell lines HepG2and SMMC7721 were used for the study. Anti-proliferative effect was measured by MTT assay, and apoptosis was determined by Hoechst-33258 staining, electronography and DNA fragmentation. The protein of cyclooxygenase-2 (COX(2) and Bcl-2 were detected by Westem dot blotting.RESULTS: Sulindac could initiate growth inhibition and apoptosis of MKN45, MKN28, HepG2 and SMMC7721 cells in a dose-and time-dependent manner. Growth inhibitory activity and apoptosis were more sensitive in HepG2 cells than in SMMC7721 cells, MKN45 and MKN28 cells. After 24hours incubation with sulindac at 2mmol. L-1 and 4mmol.L-1, the level of COX-2 and Bcl-2 protein were lowered in MKN45, SMMC7721 and HepG2 cells but not in MKN28 cells.CONCLUSION: Sulindac could inhibit the growth of gastric cancer cells and HCC cells effectively in vitro by apoptosis induction, which was associated with regression of COX-2and Bcl-2 expression. The growth inhibition and apoptosis of HCC cells were greater then that of human gastric cancer cells. The different effects of apoptosis in gastric cancer cells may be related to the differentiation of the cells. | Yun-Lin Wu~1 Bo Sun~1 Xue-Jun Zhang~2 Sheng-Nian Wang~2 Heng-Yi He~2 Min-Min Qiao~1 Jie Zhong~1 Jia-Yu Xu~1 1 Department of Gastroenterology,Ruijin Hospital,Shanghai Second Medical University,Shanghai 200025,China2 Institude of Biochemistry and Cell Biology,Shanghai Institues for Biological Sciences,Chinese Academy of Sciences.Shanghai 200025,China | 2001 | World Journal of Gastroenterology2001，7，6： | 64 |
| 2 | Formalized therapeutic guideline for hyperlipidemic severe acute pancreatitis显示文摘AIM: To investigate a formalized therapeutic guideline for hyperlipidemic severe acute pancreatitis (HL-SAP).METHODS: Thirty-two consecutive patients with severe acute pancreatitis were included in the clinical trial. All of them met the following five criteria for admission to the study, namely the Atlanta classification and stratification system for the clinical diagnosis of SAP, APACHEII score more than 8, time interval for therapeutic intervention less than 72 hours after onset of the disease, serum triglyceride (TG) level 6.8 mmol/l or over, and exdusion of other etiologies.They were divided into severe acute pancreatitis group (SAP,22 patients) and fulminant severe acute pancreatitis group (FSAP, 10 patients). Besides the conventional therapeutic measures, Penta-association therapywas also applied in the two groups, which consisted of blood purification (adsorption of triglyceride and hemofiltration), antihyperlipidemic agents (fluvastatin or lipanthyl), low molecular weight heparin (fragmin), insulin, topical application of Pixiao (a traditional Chinese medicine) over the whole abdomen, serum triglyceride,pro-inflammatory cytokines and anti-inflammatory cytokines were determined before blood purification (PF), at the end of blood purification (AFE) and on the 7th day after onset of the disease (AF7) respectively. Simultaneously, severity of the diseases was assessed by the APACHE Ⅱ system.Prognosis was evaluated by non-operation cure rate,absorption rate of pseudocyst, time interval pseudocyst absorption, hospital stay and survival rate.RESULTS: Serum triglyceride level (mmol/L), TNFα (U/mi) concentration and APACHE Ⅱ score were significantly decreased (P<0.05) at AFE and AF7, as compared with PF.However, serum IL-10 concentration (pg/ml) was increased significantly (P<0.001) at AFE, and decreased significantly (P<0.05) at AF7 when compared with PF. Operations: The First surgical intervention time was 55.8±42.6 days in SAP group (5 patients) and 12.2±6.6 days in FSAP group (7 patients),there was a significant difference between the two groups (P=0.02). The number of operations in the two groups was 1.33±0.5 vs3.5±1.2 (P=0.0037), respectively. Prognosis: Nonoperation cure rate, absorption rate of pseudocyst, hospital stay and survival rate in SAP group and FSAP group were 100 % (22/22) vs11.1% (1/9), 77.3 % (17/22) vs 11.1%(1/9), 54.2±35.9 vs99.1±49.5 days (P=0.008) and 100 %(22/22) vs66.7 % (6/9) (P=0.0044). The time for absorption of pseudocyst was 135.1±137.5 days in SAP group.CONCLUSION: Penta-association therapy is an effective guideline in the treatment of hyperlipidemic severe acute pancreatitis at its early stage (within 72 hours). | En-Qiang Mao Yao-Qing Tang Sheng-Dao Zhang, Department of Surgery, Ruijin Hospital, Shanghai Second Medical University, Shanghai 200025, China | 2003 | World Journal of Gastroenterology2003，9，11： | 64 |
| 3 | Effects of time interval for hemofiltration on the prognosis of severe acute pancreatitis显示文摘AIM: To evaluate the impact of time interval for hemofiltration(HF) on the prognosis of severe acute pancreatitis (SAP).METHODS: Thirty-six consecutive patients with severe acutepancreatitis were included in the study. Atlanta classificationsystem was applied for stratification. They were randomlydivided into short veno-venous HF group, (SWH, Group 1,20 patients); and long veno-venous HF group (LWH, Group2, 16 patients). In group 1, SWH was stopped when theabdominal signs disappeared, and heart rate and breathrate were less than 90 leats/min and 20 times/min,respectively. HF was stopped if SWH was continued, andwhen heart rate and breath rate were more than 90 beats/min and 20 times/min again (Group 2). Except that the timeinterval for HF was different, other parameters for HF werethe same. And conservative curing rate, survival rate, costfor hospital stay and length of hospital stay were observed.RESULTS: Time interval for HF in Group 1 (3.81±1.3 hr)was shorter than that of in Group 2 (9.38+2.9hr), P<0.01.Conservative curing rate (90 %) in Group 1 was much higherthan that in Group 2 (56.3 %) (P<0.05); but cost in Group 1(RMB 56 600±56 400 Yuan) was lower than that in Group 2(RMB 137 000±105 000 Yuan) (P<0.05). And the survival rate(95 %) in Group 1 was higher than that in Group 2 (81.3 %)(P<0.25); however, hospital stay in Group 1 (44.3±41 days)was shorter than that in Group 2 (55.2±39.5 days) (P<0.2).So, the prognosis was not improved through the prolongationof time interval for HF, but side-effects were seen.CONCLUSION: The prognosis was not further improvedby LWH in the treatment of SAP, with side-effects. Timeinterval for HF plays an important role in treatment of SAPin early stage. SWH is thought to be superior to LWH; andLWH is superior to CVVH in early (72hrs) treatment of SAP. | En-Qiang Mao Yao-Qing Tang Sheng-Dao Zhang Department of Surgery, Ruijin Hospital, Shanghai Second Medical University, Shanghai 200025, China | 2003 | World Journal of Gastroenterology2003，9，2： | 56 |
| 4 | An Eperimental Study on the Transplantation of Human Fetal Brain Cells into Monkey Models of Parkinson's Disease.显示文摘Unilateral administration of methly-phenyl-tetrahydropyridine(MPTP) into the common carotid | Liu Zhengou, et al. Dept Neur Ruijin, Hospital. Shanghai Second Med Uni, Shanghai 200025, Chin J Neuro Psych 1994 | 1995 | Chinese Medical Journal1995，，2： | 34 |
| 5 | Methionine-dependence and combination chemotherapy on human gastric cancer cells in vitro显示文摘AIM: To elucidate whether human primary gastric cancer and gastric mucosa epithelial calls in vitro can grow normally in a rnethionine (Met) depleted environment, i.e.Met-dependence, and whether Met-depleting status can enhance the killing effect of chemotherapy on gastric cancer cells.lMETHODS: Fresh human gastric cancer and mucosal tissueswere managed to form monocellular suspensions, whichwere then cultured in the Met-free but homocysteine-containing ( MetHcy+ ) medium, with differentchemotherapeutic drugs. The proliferation of the cells wasexamined by cell counter, flow cytometry (FCM) andmicrocytotoxicity assay (MTT).RESULTS: The growth of human primary gastric cancer cellsin Met Hcy+ was suppressed, manifested by the decrease oftotal cell counts [1.46±0.42 ( x 109@L-1) in Met-Hcy+ vs .64±0.44 ( x l09@L-1) in Met+ Hcy, P<0.01], the decline inthe percentage of G0G1 phase cells (0.69±0.24 in Met-Hey+vs 0.80±0.18 in Met+ Hcy, P<0.01) and the increase of Scells(0.24±0.20inMet-Hcy+ vs 0.17 ± 0.16 in Met+ Hcy-, P< 0.01); however, gastric mucusal cells grew normally. IfMet-Hcy+ medium was used in combination withchemotherapeutic drugs, the number of surviving gastriccancer cells dropped significantly.CONCLUSION: Human primary gastric cancer cells in vitroare Met-dependent; however, gastric mucosal cells have notshown the same characteristica. Met- Hcy+ environment maystrengthen the killing effect of chemotherapy on humanprimary gastric cancer cells. | Wei-Xin Cao Jing-Min Ou Xu-Feng Fei,Department of Clinical Nutrition,Shanghai Institute of Digestive Surgery,Ruijin Hospital,Shanghai Second Medical University,Shanghai 200025,China Zheng-Gang Zhu Hao-Ran Yin Min Yan Yan-Zhen Lin,Department of Surgery,Shanghai Institute of Digestive Surgery,Ruijin Hospital,Shanghai Second Medical University,Shangha 200025,China | 2002 | World Journal of Gastroenterology2002，8，2： | 24 |
| 6 | Laparoscopic low anterior resection for rectal carcinoma:Complications and management in 132 consecutive patients显示文摘AIM:To analyze the clinical manifestations and risk fac-tors of complications in laparoscopic low anterior resection(LAR)for rectal cancer patients.METHODS:A series of 132 consecutive patients who received laparoscopic LAR for rectal cancer in our center were included.The etiology,diagnosis,treatment and prevention of rectal cancer were studied among the patients with surgery-related complications using both univariate and multivariate regression analysis.RESULTS:No conversion to open surgery was observed and 5 cases converted to hand-assisted laparoscopic operation.The overall morbidity rate was 20.5%.Complications occurred during the operation in 7 patients(5.3%),within 30 postoperative days in 24 patients(18.2%),and within 3 mo in 2 patients(1.5%).The most significant complications were anastomotic leak-age(9.1%)and anastomotic hemorrhage(5.3%).Sizeand location of tumor,pathological staging and preoperative nutrition were significant factors associated with LAR complications,while gender,age and pathological type showed no relevance.Binary logistics regression showed that the size and location of tumor,and pathological staging were independent factors of laparoscopic LAR.All the complications were treated during their onset of clinical manifestations by interventional or conservative therapy.CONCLUSION:Anastomotic leakage is a major com-plication in laparoscopic LAR.The complications may be associated with tumor size and site,and pathological stage.Interventional therapies are of value in the management of laparoscopic LAR complications. | Qian-Lin Zhu,Bo Feng,Ai-Guo Lu,Ming-Liang Wang,Wei-Guo Hu,Jian-Wen Li,Zhi-Hai Mao,Min-Hua Zheng,Department of General Surgery,Shanghai Ruijin Hospital,Shanghai Jiao Tong University School of Medicine,Shanghai 200025,China Shanghai Minimally Invasive Surgery Center,Shanghai 200025,China | 2010 | World Journal of Gastroenterology2010，16，36： | 23 |
| 7 | p16 gene methylation in colorectal cancers associated with Duke's staging显示文摘AIM To explore the association of methylation of the CpG island in the promotor of the p16 tumor suppressor gene with the clinicopathological characteristics of the colorectal cancers.``METHODS Methylation-specific PCR (MSP) was used to detect p16 methylation of 62 sporadic colorectal cancer specimens.``RESULTS pi6 methylation was detected in 42% of the tumors. Dukes staging was associated with p16methylation status, p16 methylation occurred more frequently in Dukes C and D patients (75.9%) than in Dukes A and B patients (12.1%).``CONCLUSION p16 rnethylation plays a role in the carcinogenesis of a subset of colorectal cancer, and it might be linked to poor prognosis. | Jing Yi~1 Zhi-Wei Wang~1 Hui Cang~1 Yu-Ying Chen~1 Ren Zhao~2 Bao-Ming Yu~2 Xue-Ming Tang~1 1 Department of Cell Biology,2 Department of Surgery,Ruijin Hospital,Shanghai Second Medical University,Shanghai 200025,China | 2001 | World Journal of Gastroenterology2001，7，5： | 21 |
| 8 | Effect of N-desulfated heparin on hepatic/renal ischemia reperfusion injury in rats显示文摘AIM: To investigate the effect of N-desulfated heparin onhepatic/renal ischemia and reperfusion injury in rats.METHODS: Using rat models of 60 minutes hepatic or renalischemia followed by 1 h,3 h,6 h and 24 h reperfusion,animalswere randomly divided into following groups,the shamoperated controls,ischemic group receiving only normalsaline,and treated group receiving N-desulfated heparin ata dose of 12 mg/kg at 5 minutes before reperfusion. P-selectin expression was detected in bepatic/renal tissueswith immunohistochemistry methodRESULTS: P-selectin expression, serum ALT, AST, BUN andCr levels were significantly increased during 60 minuteischemia and 1 h, 3 h, 6 h and 24 h reperfusion,while theincrement was significantly inhibited,and hepatic/renalpathology observed by light microscopy was remarkablyimproved by treatment with the N-desulfated heparin.Furthermore,the heparin was found no effects on PT and KPTT.CONCLUSION: P-selectin might mediate neutrophilinfiltration and contribute to hepatic/renal ischemia andreperfusion. The N-desulfated heparin might preventhepatic/renal damage induced by ischemia and reperfusioninjury without significant anticoagulant activity. | Tong Zhou Wei Song Feng Wang Department of Nephrology,Ruijin Hospital,Shanghai Second Medical University,Shanghai 200025,China Jin-Lian Chen Department of Gastroenterology,Shanghai Sixth People’s Hospital,Shanghai 200233,China Ming-Jun Zhang Animal Laboratory,Ruijin Hospital,Shanghai Second Medical University,Shanghai 200025,China Pei-Hua Ni,Department of Biochemistry,Shanghai Second Medical University,Shanghai 200025 Jian-Guo Geng Institute of Biochemistry and Cell Biology,Shanghai 200030,China | 2002 | World Journal of Gastroenterology2002，8，5： | 20 |
| 9 | Effects of epidermal growth factor on the growth of human gastric cancer cell and the implanted tumor of nude mice显示文摘AIM: Epidermal growth factor (EGF) plays an important rolein the regulation of gastrointestinal tissue growth anddevelopment, and it can stimulate epithelial proliferation,cell differentiation and growth. It has been established thatthe EGF can promote gastric cytoprotection and ulcerhealing. But the potential ability of EGF to regulate thegastric cancer growth is unknown. This study is toinvestigate the influence of EGF on human gastric cancercell and the implanted tumor growth of nude mice.METHODS: The cell growth rates of human gastricadenocarinoma cell lines MKN-28, MKN-45, SGC-7901 andnormal human gastric epithelial cells 3T3 were assessedwhen incubated with recombinant human EGF (rhEGF, 0.05, 0.1, 0.5, 1.0, 10, 50, 100 mg.L-1) using MTT method.The cells of MKN-28, MKN-45, SGC-7901 (gaatric cancertissue 1.5 mm3 ) were implanted in the BALB/cA nude micefor 10 days. The EGF was given intrapsritoneally (15, 30, 60μg. kg-1) for 3 weels. The body weights of the tumor-bearing animals and their tumor mass were measuredafterwards to assess the mitogenic effect of rhEGF in thenude mice.RESULTS: Within the concentration range of 0.05-100 mg.L-1 , rhEGF could increase the cell growth ofnormal 3T3 cells(cell growth rate 100 % vs 102.8 %, P<0.05), but partiallyrestrain the gastric cancer cell growth. The latter effect wesrelated to cell differentiation. In 15-60μg/kg rhEGF groups,the mean implanted tumor mass of MKN-28 cell were 1.75 g,1.91 g, 2.08 g/NS group 1.97 g ( P> 0.05), the mean tumormass of SGC-7901 cell were 1.53 g, 1.07 g, 1.20 g/NS group1.07 g ( P > 0.05), and for MKN-45 cell, the tumor masswere respectively 1.92 g, 1.29 g, 1.77 g/NS group 1.82 g( P> 0.05 ). So rhEGF had no obvious effect on implantedMKN-28, SGC-7901 and MKN-45 tumor growth.CONCLUSION: EGF has no stimulating effect on the humangastric cancer cell growth neither in vitro nor in vivo. | Lu Xia Yao-Zong Yuan Chun-Di Xu Yong-Pin Zhang Ming-Ming Qiao Jia-Xu Xu,Department of Gastroenterology,Ruijin Hospital,Shanghai Second Medical University,Shanghai 200025,China | 2002 | World Journal of Gastroenterology2002，8，3： | 14 |
| 10 | 上海市9所医院前瞻性胃癌前病变5年随访研究显示文摘对上海市9所医院的2000例胃癌前变化进行胃镜随访研究。随访时间:第一部分从1986年1月~1990年3月;第二部分从1986年1月~1993年6月。随访组每6~12个月定期复查胃镜,对照组不进行主动胃镜检查,以积分法观察癌前变化。结果:第一部分随访组胃癌发生率为1.87%(17/909),其中早期胃癌占70.6%(12/17);对照组胃癌发生率为1.25%(9/721),其中早期胃癌占11.1%(1/9)。随访组病理积分从4.25降至2.95。第二部分仅发现1例早期胃癌(1/261)。本研究提示,加强对胃癌高危人群的胃镜随访是早期发现胃癌的有效方法。 | Wu Yuxin, Jiang Shihu, Yuan Yaozong. Dept.of Medicine,Ruijin Hospital,Shanghai Second Medical University. | 1995 | 中华消化杂志1995，15，5： | 14 |
| 11 | Combined human growth hormone and lactulose for prevention and treatment of multiple organ dysfunction in patients with severe chronic hepatitis B显示文摘AIM: To evaluate the efficiency and safety of combined recombinant human growth hormone (rhGH) and lactulose for treatment and/or prevention of multiple organ dysfunction in patients with chronic severe hepatitis B. METHODS: Forty-eight inpatients with chronic severe hepatitis B were randomly divided into rhGH group (n = 28)and control group (n = 20). In rhGH group, 4-4.5 IU of rhGH was injected intramuscularly once daily for 2-4 wk,and 100 mL of enema containing 30 mL of lactulose, 2 g of metronidazole and 0.9% saline was administered every 2 d for 2-4 wk. Their symptoms and complications were noted. Liver and kidney functions were analyzed by an Olympus analyzer. Serum GH, IGF-1, IGFBP1 and IGFBP3 were measured by ELISA.RESULTS: Clinical symptoms of 90% of these patients in rhGH group were obviously improved. The total effectiveness in rhGH group was better than that in control group (75% vs40%, P<0.05). After 2- and 4-wk treatment of rhGH respectively, serum albumin (26.1±4.1 vs 30.2±5.3,31.9±5.1 g/L), prealbumin (79.6±28.0 vs 106.6±54.4,108.4±55.0 g/L), cholesterol (76.3±16.7 vs 85.6±32.3,96.1±38.7 mg/dL), and IGFBP1 (56.8±47.2 vs 89.7±50.3ng/mL after 2 wk) were significantly increased compared to control group (P<0.05). However, serum GH was decreased. The increase of serum IGF1 and IGFBP3 after rhGH treatment was also observed.CONCLUSION: rhGH in combination with lactulose may be beneficial to the prevention and treatment of multiple organ dysfunction in patients with chronic severe hepatitis. | Hui-GuoDing JingShan BinZhang Hong-BoMa LiZhou RuiJin Yu-FenTan Li-XiangHe | 2005 | World Journal of Gastroenterology2005，11，19： | 13 |
| 12 | Research on the feature extraction of DC space charge behavior of oil-paper insulation显示文摘As the ultra high DC voltage grid comes into an important stage in the ‘Twelfth Five-year Plan’ of China, the requirement on the operation safety of power equipment needs to be improved. Oil-paper insulation plays a significant role as the main insulation material of many key power equipments. The space charge behaviors of oil-paper insulation not only affect the dielectric strength, but also relate to the stability and security of power transmission facilities. Until now, the research on the space charge behaviors of oil-paper insulation remains on the stage of objective description and phenomenon explanation, thus it is very urgent to make some feature extractions on the test results in order to further investigate the DC space charge behaviors of oil-paper insulation. In this paper, based on the large numbers of test data in the first stage, data mining and feature extractions were performed on the DC space charge behavior of oil-paper insulation. The variation laws of some key characteristic parameters obtained in different experiment conditions such as the total charge injection amount, apparent charge mobility, and trap energy distribution were analyzed. In addition, the distribution of trapping level inside oil-paper was calculated by using the PEA test results based on the mechanism of the isothermal decay current, which realized the approximate calculation of trap depth by charge decay characteristics. What’s more, the exponential injection and decay equations of DC space charge changing with time were acquired for the first time, and the physical meanings of related parameters were defined. This has laid a foundation for the prediction and simulation of space charge in the oil-paper insulation. | TANG Chao LIAO RuiJin CHEN George YANG LiJun | 2011 | Science China(Technological Sciences)2011，54，5： | 13 |
| 13 | Simultaneous laparoscopy-assisted low anterior resection and distal gastrectomy for synchronous carcinoma of rectum and stomach显示文摘Laparoscopic resection of rectal cancer or gastric cancer has been advocated for the benefits of a reduced morbidity,a shorter treatment time,and similar outcomes.However,simultaneous laparoscopy-assisted low anterior resection and distal gastrectomy for synchronous carcinoma of rectum and stomach are rarely documented in literature.Endoscopic examination revealed a synchronous carcinoma of rectum and stomach in a 55-year-old male patient with rectal bleeding and epigastric discomfort.He underwent a simultaneous laparoscopy-assisted low anterior resection and distal gastrectomy with regional lymph nodes dissected.The operation time was 270 min and the estimated blood loss was 120 mL.The patient required parenteral analgesia for less than 24 h.Flatus was passed on postoperative day 3,and a solid diet was resumed on postoperative day 7.He was discharged on postoperative day 13.With the advances in laparoscopic technology and experience,simultaneous resection is an attractive alternative to a synchronous gastrointestinal cancer. | Qian-Lin Zhu,Min-Hua Zheng,Bo Feng,Ai-Guo Lu,Min-Liang Wang,Jian-Wen Li,Wei-Guo Hu,Lu Zang,Zhi-Hai Mao,Feng Dong,Jun-Jun Ma,Ya-Ping Zong,Department of General Surgery,Ruijin Hospital Affiliated to Shanghai Jiaotong University Shanghai Minimally Invasive Surgery Center,Shanghai 200025,China | 2008 | World Journal of Gastroenterology2008，14，21： | 11 |
| 14 | Influence of L-methionine-deprived total parenteral nutrition with 5-fluorouracil on gastric cancer and host metabolism显示文摘AIM To investigate the influence of L-methioninedeprived total parentaral nutrition with 5-FU on gastric cancer and host metabolism.``METHODS N-methyI-N-nitro-nitrosoguanidine ( MNNG )induced gastric cancer rats were randomly divided into four groups: Met-containing TPN group (n= 11 ), Metdeprived TPN group (n = 12), Met-containing TPN + 5-FU group (n = 11) and Met-deprived TPN + 5-FU group (n-12). Five rats in each group were sacrificed after 7days of treatment and the samples were taken for examination. The remaining rats in each group were then fed separately with normal diet after the treatment until death, the life span was noted.``RESULTS The tumors were enlarged in Met-containing group and shrank in Met-deprived group markedly after the treatment. The DNA index (DI) of tumor cells and the body weight (BW) of rats had no significant change in the two groups, however, the ratio of tumor cells' S phase was increased The ratio of G2M phase went up in Metcontaining group, but down in Met-deprived group. In the other two groups that 5-FU was added, the BW of rats,and the diameter of tumors, the DI of tumor cells, the S and G2M phase ratio of tumor cells were all decreased,particularly in Met-deprived plus 5-FU group. Pathological examination revealed that the necrotic foci of the tumor tissue increased after Met-deprived TPN treatment, and the nucleoli of tumor cells enlarged. In -MetTPN + 5-FUgroup, severe nuclear damage was also found by karyopyknosis and karyorrhexis, meanwhile there was slight degeneration in some liver and kidney cells. The serum free Met and Cysteine decreased markedly (P<0.001), while other amino acids, such as serum free serine and glutamine increased significantly ( P< 0.005).All the rats died of multiple organ failure caused by cancer metastasis. The average survival time was 18.6 days in Met-containing TPN group. 31 days in Met-deprived TPN group, 27.5 days in Met-containing TPN + 5-FU group, and 43 days in Met-deprived TPN+ 5-FU group (P<0.05).``CONCLUSION Met-deprived TPN causes methionine starvation of tumor cells, and can enhance the anti-tumor effect of 5-FU and prolong the life span of gastric cancerbearing rats. | Hong-Bing Xiao~1 Wei-Xin Cao~2 Hao-Ran Yin~2 Yan-Zhen Lin~2 Shi-Hui Ye~1 1 Department of Surgery,Affiliated Railway Hospital,Tongji University,Shanghai 200072,China2 Department of Surgery,Affiliated Ruijin Hospital,Shanghai Second Medical University,Shanghai 200025,China | 2001 | World Journal of Gastroenterology2001，7，5： | 9 |
| 15 | Reasonable choice of surgical procedures for patients with portal hypertension显示文摘OBJECTIVE: To assess individualized therapeutic protocol for patients with portal hypertension on thebasis of accumulated knowledge about the mechanism of portal hypertension.DATA SOURCES: Patients data on shunt and other surgical procedures from Ruijin Hospital, Shanghai,China and the published papers.RESULTS: The direction of blood flow of the collateral vessels in the gastro-splenic region is animportant factor in deciding surgical strategy because there is a close relationship between surgical riskand the classification of liver function. Clinically it is confirmed that each patient needs an individualizedsurgical procedure and that prophylactic operation is suitable for patients with splenomegaly, splenismassociated with serious esophageal varices and hemorrhagic tendency under endoscopy but acceptableliver function. The shunt diameter (SD) (SD=0.67×PVD) is determined in our patients according toindividualized hemodynamics. The rehemorrhagic rate after shunt being higher than that in others may berelated to lesioned gastric mucosa caused by portal hypertension or bleeding and temporary melena. Thisfinding is good for prevention of hepatic encephalopathy. The life quality and labor ability of patients willbe improved because of hepatopetal flow in the portal vein. With strict indications for reoperation,selective operation is performed as soon as possible when hemorrhage is controlled conservatively andliver function improved. Once the patient with cirrhosis associated with portal hypertension is scheduledfor liver transplantation, treatment of hemorrhage should aim to keep the patient in good condition and toavoid the protocol that may be disadvantageous to liver transplantation in the future.CONCLUSION: Surgical procedures for patients with portal hypertension should follow the principle ofindividualization. To obtain the best outcome, the choice of reasonable surgical procedure is expected. | Guang-Wen Zhou Zong-Yuan Tao Cheng-Hong Peng Hong-Wei Li the Surgical Department and Shanghai Digestive Surgical Institute, Ruijin Hospital, Shanghai Second Medical University, Shanghai 200025, China | 2003 | Hepatobiliary & Pancreatic Diseases International2003，2，3： | 8 |
| 16 | Rapid detection of sepsis complicating acute necrotizing pancreatitis using polymerase chain reaction显示文摘INTRODUCTIONAcute narcotizing pancreatitis usually takes a severe clinical course and is associated with multiple organ dysfunction .With the further understanding of pathophysiological events of acute pancreatisis and the therapeutic measuses taken by the clinicians ,the patients can pass through the critical carry stages ,and then the septic complication caused by rtanslocated bacteria, mostly gram-negative microbes from the intestines ensues[1]. | Wei Zhong Zhang1 Tian Quan Han2 Yao Qing Tang2 Sheng Dao Zhang2 1Department of Surgery. Huangyan First Hospital, Huangyan 318020, Zhejiang Province. China 2Department of Surgery. Ruijin Hospital. Shanghai Second Medical University. Shanghai 200025. ChinaDr. Wei Zhong Zhang, graduated from Shanghai Second MedicalUniversity receiving master degree of surgery in 1999 he is devoted to basic and clinical investigation on severe acute pancreatitis and has one paper published. | 2001 | World Journal of Gastroenterology2001，7，2： | 7 |
| 17 | Diagnosis and treatment of solidpseudopapillary tumors of the pancreas显示文摘Solid-pseudopapillary tumors (SPTs) of the pancreas have been reported as rare lesions with' low malignant potential' occurring mainly in young women. This study was designed to define the clinicopathological characteristics and the effect of surgical intervention. METHODS:A retrospective clinical analysis was made of 21 patients with solid-pseudopapillary tumor of the pancreas admitted from June 1994 to December 2004. RESULTS:Abdominal pain as the major complaint was found in 13 patients, and palpable abdominal mass in 7. Imaging examination showed the abdominal mass clearly, but diagnosis was not defined. In 12 patients, tumors were located in the pancreatic head and in 9 patients, in the pancreatic tail. The average diameter of the tumor was 9.5 cm (range, 3-20 cm). One patient had liver metastasis. The diagnosis of the tumor was proved pathologically during operation in 14 patients, and other 3 patients who had been diagnosed as having insulinoma and other malignant tumors were confirmed by paraffin section and enzyme labeling after operation. Eighteen patients (85.7% ) were followed up with a median period of 24 months, 1-60 months without tumor recurrence. CONCLUSION:Huge pancreatic masses of the pancreas in young women are suggestive of solid-pseudopapillary tumors. Aggressive surgical therapy can result in good prognosis in these patients. | Xi Chen, Guang-Wen Zhou, Hui-Jiang Zhou, Cheng-Hong Peng and Hong-Wei Li Department of Surgery, Shanghai Institute of Digestive Surgery, Ruijin Hospital, Shanghai Second Medical University, Shanghai 200025, China | 2005 | Hepatobiliary & Pancreatic Diseases International2005，4，3： | 7 |
| 18 | Preparation and characterization of chitosan porous microcarriers for hepatocyte culture显示文摘BACKGROUND: The bioartificial liver (BAL) is considered a possible alternative method for treating liver failure. The core of the BAL system is culturing liver cells in vitro with high density and activity. Microcarrier culture is a mode of high-density culture. We set out to prepare a novel porous microcarrier to improve the activity of liver cells in vitro. METHODS: Chitosan was used to prepare a novel porous spherical microcarrier with interconnected structure. The chitosan porous microcarriers (CPMs) were modified with gelatin to improve their biocompatibility. CPMs were co-cultured with liver cells, HL-7702 (L-02), to evaluate their effect on cell culture. RESULTS: The average size of the CPMs was about 400 μm in diameter and their apertures were less than 30 μm. The pores of the microcarrier were interconnected. After fixation by sodium tripolyphosphate, the structure of the first freeze-dried CPMs was stable. To further improve the biocompatibility, the surface of CPMs was modified with gelatin through chemical crosslinking (GM-CPMs). Comparing the proliferation curves of L-02 cells cultured on simple CPMs, GM-CPMs and tissue culture polystyrene (TCPS, a mode of planar cell culture), the proliferation rates were similar in the first 5 days and the cells proliferated until day 8 in culture with microcarriers. The OD value of liver cells cultured on GM-CPMs was 1.97-fold higher than that on TCPS culture at day 8. Levels of urea and albumin in supernatants of cells cultured on GM-CPMs increased steadily for 8 days, and were clearly higher than those of cells cultured on TCPS (P<0.05).CONCLUSIONS: The novel CPMs were promising microcarriers for hepatocyte culture and the GM-CPM seemed better. Porous microcarrier culture was beneficial for hepatocyte function and activity. | Xu-Bo Wu, Cheng-Hong Peng, Fang Huang, Jie Kuang, Song-Lin Yu, Ya-Dong Dong and Bao-San Han Center of Organ Transplantation, Ruijin Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 200025, China Department of General Surgery, Central Hospital, Minhang District, Shanghai 201100, China | 2011 | Hepatobiliary & Pancreatic Diseases International2011，10，5： | 6 |
| 19 | Study on the function of pharynx & upper esophageal sphincter in globus hystericus显示文摘AIM: Globus pharyngeus is not an uncommon symptom.Presently, its unclear dated pathophysiology remains unclearand the disease can not be evaluated correctly with routinediagnostic methods. The objective of this study is to establishthe normal values of pharyngeal and UES pressure,pharyngeal transit time in healthy volunteers and to comparethe differences betweer healthy volunteers and patients withglobus pharyngeus.METHODS: Twenty-four healthy volunteers and thirty-twopatients clinically diagnosed as globus pharyngeus enteredthe study. Pressures of pharynx and UES were measured.Pharyngeal transit time was measured by videofluoroscopicprocedure.RESULTS: Normal pressure of pharynx, and normal restingpressure of UES were 157.81±63.86 mmHg and 68.33±37.56 mmHg, respectively. The corresponding values inthe patients were 175.50±93.47 mmHg and 71.38±41.42mmHg. The pharyngeal transit time was 1.44±0.30 s innormal control group, among them there were 4 caseswith stasis of barium in the valleculae and one in the piriformsinus. No laryngeal penetration or aspiration was found.In the patient group, the pharyngeal transit time was 1.37±0.41 s, among them there were 6 cases with stasis ofbarium in the valleculae and 5 in the piriform sinus. 9 caseshad laryngeal penetration and 2 had aspiration. There wereno statistical differences of pressures of pharynx, UES andthe pharyngeal transit time between the two groups. Butthere was an association between laryngeal penetrationand globus pharyngeus.CONCLUSION: Radiographic examination of the pharynxshow specific findings of pharyngeal dysfunction in patientswith globus pharyngeus. UES pressure is normal in mostpatients. Hence, we find no role for UES hypertonicity as anetiologic factor in globus pharyngeus. | Jing Sun Bin Xu Yao-Zong Yuan Jia-Yu Xu Department of Gastroenterology,Ruijin hospital,Shanghai Second Medical University,Shanghai 200025,China | 2002 | World Journal of Gastroenterology2002，8，5： | 6 |
| 20 | Pancreatectomy combined with superior mesenteric-portal vein resection: report of 32 cases显示文摘BACKGROUND: Resection of the superior mesenteric- portal vein (SMPV) during pancreatoduodenectomy is dis- puted. Although the morbidity and mortality of patients af- ter this operation are acceptable, survival is limited. In this study, we evaluated the morbidity, mortality and survival of patients with ductal adenocarcinoma of the pancreas who had undergone pancreatectomy with en bloc portal vein re- section. METHODS: A total of 32 patients with ductal adenocarci- noma of the pancreas who had undergone pancreatectomy with SMPV resection between 1999 and 2003 were retro- spectively analyzed. In addition, they were categorized in- to two groups according to the invasion of the wall of the portal vein: group A (n =12), extended compression of the wall of the portal vein by surrounding carcinoma without true invasion and group B (n =20), true invasion including intramural and transmural invasion. RESULTS; The morbidity of the 32 patients was 31.25%. There was no operative death, and the overall 1-,3-year survival rates were 59% and 16%, respectively. The mean survival time of patients with microscopically positive mar- gin was only 5. 6 months as compared with 20 months in patients with microscopically negative margin. No diffe- rences in tumor size, margin positivity, nodal positivity, and 1-, 3-year survival rates were observed between the two groups. CONCLUSIONS; If selected carefully, pancreatectomy combined with SMPV resection can be performed safely, without increase in the morbidity and mortality. SMPV re- section should be performed only when a margin-negative resection is expected to be achieved. SMPV invasion is notassociated with histologic parameters suggesting a poor prognosis. | Guang-Wen Zhou, Wei-Ding Wu, Wei-Dong Xiao, Hong-Wei Li and Cheng-Hong Peng Shanghai, China Department of Surgery, Shanghai Institute of Diges- tive Surgery, Ruijin Hospital, Shanghai Second Medical University, Shang- hai 200025, China | 2005 | Hepatobiliary & Pancreatic Diseases International2005，4，1： | 6 |
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