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| 1 | 荧光多重实时PCR检测单纯疱疹病毒显示文摘目的建立单纯疱疹病毒的荧光多重实时PCR检测及分型技术,为生殖器疱疹的诊断及病毒分型提供快捷、敏感、特异的方法.方法以细胞培养法为对照,用Smart Cycler System作为实验平台,以DNA探针荧光标记技术建立荧光多重实时PCR,用于检测生殖器疱疹患者临床标本中的1型和2型单纯疱疹病毒(HSV1、HSV2)DNA.结果荧光多重实时PCR检测HSV1和HSV2 DNA的敏感性和特异性均为100%,可检测出低至1~5拷贝的HSV DNA,30 min左右即可完成整个检测过程,而细胞培养的敏感性和特异性分别为70.10%和100%.结论基于Smart Cycler System的荧光多重实时PCR是一种易于操作、扩增效率高、封闭式的核酸扩增技术,可用一次PCR反应就能快速、敏感、准确地对临床标本中的单纯疱疹病毒进行检测和分型. | 赖维 Cheng-Yen Chen 苏向阳 黄怀球 张玉清 Ron C Ballard Stephen A Morse | 2004 | 中华皮肤科杂志2004，37，5： | 6 |
| 2 | Dynamic chromatin states in human ES cells reveal potential regulatory sequences and genes involved in pluripotency显示文摘Pluripotency,一个细胞的能力区分并且产生所有胚胎的系,定义象胚胎的茎(ES ) 那样的哺乳动物的细胞类型的一个小数字细胞。当它通常被保持了时,那 pluripotency 是 transcriptional 的产品激活并且维持关键干细胞基因的表达式的规章的网络,积累的证据在建立并且保卫 ES 房间的 pluripotency 为 epigenetic 进程正在指向一个关键角色,象维持区分的房间类型的身份一样。以便更好在 pluripotency 理解 epigenetic 机制的角色,我们检验了在经历区别进一个 mesendodermal 系的人的 ES 房间(hESCs ) 染色体宽的染色质修正的动力学。我们发现在倡导者的染色质修正在区别期间仍然保持大部分不变,除了在在在 H3K27 的 acetylation 和 methylation 之间的一个动态开关标记在基因表示的激活和 silencing 之间的转变的倡导者的一个小数字,建议在在大多数差别上的房间命运承诺的一个层次表示了基因。我们也在 50 000 潜在的 enhancers 上印射,并且在染色质修正,特别 H3K4me1 和 H3K27ac 观察了大得多的动力学,它与他们的潜在的目标基因的表示相关。这些 enhancers 的进一步的分析揭示了 pluripotency 和可以在 hESCs 授与发展胜任的一个平衡状态的一口染色质签名陈述语气的潜在地关键的 transcriptional 管理者。我们的结果提供在定义 enhancers 和 pluripotency 支持染色质修正的角色的新证据。 | R David Hawkins Gary C Hon Chuhu Yang Jessica E Antosiewicz-Bourget Leonard K Lee Que-Minh Ngo Sarit Klugman Keith A Ching Lee E Edsall Zhen Ye Samantha Kuan Pengzhi Yu Hui Liu Xinmin Zhang Roland D Green Victor V Lobanenkov Ron Stewart James A Thomson Bing Ren | 2011 | Cell Research2011，21，10： | 6 |
| 3 | Characterization and target identification of a DNA aptamer that labels pluripotent stem cells显示文摘 | Zhonggang Hou Susanne Meyer Nicholas E Propson jeff Nie Peng Jiang Ron Stewart James A Thomson | 2015 | Cell Research2015，25，3： | 5 |
| 4 | Synchronous electrogastrographic and manometric study of the stomach as an esophageal substitute显示文摘AIM: To investigate the electric and contractile mechanisms involved in the deranged function of the transposed stomach in relation to the course of the symptoms and the changes in contractile and electrical parameters over time.METHODS: Twenty-one patients after subtotal esophagectomy and 18 healthy volunteers were studied.Complaints were compiled by using a questionnaire, and a symptom score was formed. Synchronous electrogastrography and gastric manometry were performed in the fasting state and postprandially.RESULTS: Eight of the operated patients were symptomfree and 13 had symptoms. The durations of the postoperative periods for the symptomatic (9.1±6.5 mo)and the asymptomatic (28.3±8.8 mo) patients were significantly different. The symptom score correlated negatively with the time that had elapsed since the operation. The percentages of the dominant frequency in the normogastric, bradygastric and tachygastric ranges differed significantly between the controls and the patients.A significant difference was detected between the power ratio of the controls and that of the patients. The occurrence of tachygastria in the symptomatic and the symptom-free patients correlated negatively both with the time that had elapsed and with the symptom score. There was a significant increase in motility index after feeding in the controls, but not in the patients. The contractile activity of the stomach increased both in the controls and in the symptom-free patients. In contrast, in the group of symptomatic patients, the contractile activity decreased postprandially as compared with the fasting state.CONCLUSION: The patients' post-operative complaints and symptoms change during the post-operative period and correlate with the parameters of the myoelectric and contractile activities of the stomach. Tachygastria seems to be the major pathogenetic factor involved in the contractile dysfunction. | Ferenc Izbéki Tibor Wittmann Sándor (O|')dor Balázs Botos (A|')ron Altorjay | 2005 | World Journal of Gastroenterology2005，11，8： | 2 |
| 5 | The Strengths and Difficulties Self-Report Questionnaire as a screening instrument in Norwegian community samples显示文摘 | Ronning J A Handegaard BH Sourander A | 2004 | Eur Child Adolesc Psychiatry2004，13，2： | 1 |
| 6 | Drug interaction between St John's wort and nevirapine显示文摘 | de Maat Monique MR Hoetelmans Richard M W Mathot Ron A A | 2001 | AIDS2001，15，3： | 1 |
| 7 | The structure of finitely generated shift-invariant spaces in L2 (Rd) 显示文摘 | De BOOR C De VORE R RON A | 1994 | Journal of Functional Analysis1994，119，1： | 1 |
| 8 | The Strengths and Difficulties Self-Report Questionnaire as a screening instrument in Norwegian community samples显示文摘 | Ronning JA Handegaard BH Sourander A | 2004 | Eur Child & Adolesc Psychiatry2004，13，2： | 1 |
| 9 | Self-assembled fmoc-peptides as a platform for the formation of nanostructures and hydrogels 显示文摘 | Ron O Lihi A A Sivan Z | 2009 | Biomacromolecules2009，10，9： | 1 |
| 10 | Weyl-Heisenberg flames and Riesz bases in L2(Rd)显示文摘 | RON A SHEN Zuo-wei | 1997 | Duke Math J1997，89，： | 1 |
| 11 | Simulink model for economic analysis and envi- ronmental impacts of a PV with diesel-battery system for remote village 显示文摘 | Wies Richard W Johnson Ron A Agrawal Ashisb N | 2005 | IEEE Transactions on Power Sys- tems2005，20，2： | 1 |
| 12 | Framelets: MRA-based Constructions of Wavelet Frames 显示文摘 | Daubechies I Han B Ron A | 2003 | Applied Computational Harmonic Analysis2003，14，1： | 1 |
| 13 | Does the first file to bind correspond to the diameter of the canal in the apical region?显示文摘 | Wu MK Barids D Rons A | 2002 | Int Endod J2002，35，3： | 1 |
| 14 | Calcultion of stribeck curves for water lubricated journal bearings 显示文摘 | Ales de Kraker Ron A J | 2007 | Tribology International2007，40，3： | 1 |
| 15 | The receptor for the fungal elicitor ethylene- inducing xylanase is a member of a resistance-like gene fami- ly in tomato显示文摘 | Ron M Avni A | 2004 | Plant Cell2004，16，6： | 1 |
| 16 | Affine systems in L2(R^d) : the analysis of the analysis operator显示文摘 | Ron A Shen Z | 1997 | Funct Anal1997，148，2： | 1 |
| 17 | Affine systems in L2 (Rd), the analysis of the analysis operator 显示文摘 | RON A SHEN Z | 1997 | Funct Anal1997，148，2： | 1 |
| 18 | Ovarian hyperstimulation syndrome: an update review显示文摘 | Golan A Ron EL R Herman A | 1989 | Obstet Gynecol Surv1989，44，6： | 1 |
| 19 | Amperometric quantification of total coliforms and specific detection of Escherichia coli显示文摘 | Mittelmann A S Ron E Z Rishpon J | 2002 | Analytical Chemistry2002，74，4： | 1 |
| 20 | Trade Intensity and Business Cycle Sychronization:are Developing Countries Any Different 显示文摘 | Caldéron C Chong A Stein E | | 0，，01： | 1 |
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