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| 1 | 荧光多重实时PCR检测单纯疱疹病毒显示文摘目的建立单纯疱疹病毒的荧光多重实时PCR检测及分型技术,为生殖器疱疹的诊断及病毒分型提供快捷、敏感、特异的方法.方法以细胞培养法为对照,用Smart Cycler System作为实验平台,以DNA探针荧光标记技术建立荧光多重实时PCR,用于检测生殖器疱疹患者临床标本中的1型和2型单纯疱疹病毒(HSV1、HSV2)DNA.结果荧光多重实时PCR检测HSV1和HSV2 DNA的敏感性和特异性均为100%,可检测出低至1~5拷贝的HSV DNA,30 min左右即可完成整个检测过程,而细胞培养的敏感性和特异性分别为70.10%和100%.结论基于Smart Cycler System的荧光多重实时PCR是一种易于操作、扩增效率高、封闭式的核酸扩增技术,可用一次PCR反应就能快速、敏感、准确地对临床标本中的单纯疱疹病毒进行检测和分型. | 赖维 Cheng-Yen Chen 苏向阳 黄怀球 张玉清 Ron C Ballard Stephen A Morse | 2004 | 中华皮肤科杂志2004，37，5： | 6 |
| 2 | Dynamic chromatin states in human ES cells reveal potential regulatory sequences and genes involved in pluripotency显示文摘Pluripotency,一个细胞的能力区分并且产生所有胚胎的系,定义象胚胎的茎(ES ) 那样的哺乳动物的细胞类型的一个小数字细胞。当它通常被保持了时,那 pluripotency 是 transcriptional 的产品激活并且维持关键干细胞基因的表达式的规章的网络,积累的证据在建立并且保卫 ES 房间的 pluripotency 为 epigenetic 进程正在指向一个关键角色,象维持区分的房间类型的身份一样。以便更好在 pluripotency 理解 epigenetic 机制的角色,我们检验了在经历区别进一个 mesendodermal 系的人的 ES 房间(hESCs ) 染色体宽的染色质修正的动力学。我们发现在倡导者的染色质修正在区别期间仍然保持大部分不变,除了在在在 H3K27 的 acetylation 和 methylation 之间的一个动态开关标记在基因表示的激活和 silencing 之间的转变的倡导者的一个小数字,建议在在大多数差别上的房间命运承诺的一个层次表示了基因。我们也在 50 000 潜在的 enhancers 上印射,并且在染色质修正,特别 H3K4me1 和 H3K27ac 观察了大得多的动力学,它与他们的潜在的目标基因的表示相关。这些 enhancers 的进一步的分析揭示了 pluripotency 和可以在 hESCs 授与发展胜任的一个平衡状态的一口染色质签名陈述语气的潜在地关键的 transcriptional 管理者。我们的结果提供在定义 enhancers 和 pluripotency 支持染色质修正的角色的新证据。 | R David Hawkins Gary C Hon Chuhu Yang Jessica E Antosiewicz-Bourget Leonard K Lee Que-Minh Ngo Sarit Klugman Keith A Ching Lee E Edsall Zhen Ye Samantha Kuan Pengzhi Yu Hui Liu Xinmin Zhang Roland D Green Victor V Lobanenkov Ron Stewart James A Thomson Bing Ren | 2011 | Cell Research2011，21，10： | 6 |
| 3 | Incidence of brain metastasis in patients with esophageal carcinoma显示文摘AIM:To determine the incidence of brain metastasis in a contemporary group of patients with carcinoma of the esophagus.METHODS:Retrospective analysis of 53 patients with esophageal carcinoma who received radiotherapy as a component of treatment between 1998 and 2007,including patient and tumor characteristics,and subsequent diagnosis of brain metastasis.The association between the histological type of esophageal cancer and the incidence of brain metastasis was assessed using Fisher's exact test.RESULTS:Forty-four of the fifty-three patients in this study had adenocarcinoma and nine had squamous cell carcinoma,ranging from stage ⅡA-ⅣB.Primary treatment was surgery with neoadjuvant chemoradiotherapy (trimodality therapy) in 19% of patients;chemoradiotherapy in 42%;and surgery and adjuvant radiotherapy in 7%.Twenty-five percent of patients inthis study received palliative radiotherapy.The overall incidence of brain metastasis in this cohort was 13%.Adenocarcinoma was the primary tumor histology in all of the patients who developed brain metastasis,representing an incidence of 16% in this subgroup.No patients with squamous cell carcinoma received trimodality therapy.The association between histology and brain metastasis was not statistically significant.CONCLUSION:The incidence of brain metastasis in this contemporary cohort of patients with esophageal carcinoma is higher than previously reported and was confined to those with adenocarcinoma. | Ron S Smith Robert C Miller | 2011 | World Journal of Gastroenterology2011，17，19： | 6 |
| 4 | Organizational decision support system for effective R&D project selection 显示文摘 | TIAN Q MA J LIANG J RON C W K OU L | 2005 | Decision Support Systems2005，39，3： | 1 |
| 5 | Ion beam synthesis of diamond-like carbon thin films containing copper nanocrystals 显示文摘 | Gerhards I Ronning C Hofsass H | 2003 | Journal of Applied Physics2003，93，2： | 1 |
| 6 | The structure of finitely generated shift-invariant spaces in L2 (Rd) 显示文摘 | De BOOR C De VORE R RON A | 1994 | Journal of Functional Analysis1994，119，1： | 1 |
| 7 | Nonradiation risk factors for thyroid cancer in the US Radiologic Teehnol ogistsStudy显示文摘 | Meinhold C L Ron E Schonfeld S J | 2010 | Am J Epidemiol2010，171，2： | 1 |
| 8 | Ion beam synthesis of amorphous carbon thin films containing metallic nanoclusters 显示文摘 | Gerhards I Ronning C Vetter U | 2002 | Surface and Coating Technology2002，，： | 1 |
| 9 | Autologous olfactory ensheathing cell transplantation in human spinal cord injury显示文摘 | Féron F Perry C Cochrane J | | 0，，12： | 1 |
| 10 | 成人2型糖尿病不同筛查及预防策略成本效益分析显示文摘目的 比较针对2型糖尿病预防及治疗的4种筛查策略及随后予以的干预措施:(a)筛查2型糖尿病患者使其能接受早期检查及治疗,(b)筛查2型糖尿病和糖耐量受损患者,对糖耐量受损的人群予以生活方式干预以延缓或预防糖尿病的发生,(c)同(b)但予以药物干预措施,(d)不筛查。
设计 成本效益决策分析建立在对概率的发展和评估基础上,是从筛查到死亡的综合性经济决策分析模型。
研究对象 一组假设人群,筛查时年龄45岁,高于平均糖尿病风险水平。
数据来源 电子书目数据库收录的已发表的临床试验和流行病学研究;补充数据分别来自英国和威尔士健康统计署、风险筛查(STAR)研究和ADDITION研究的Leicester部分。
方法 建立一种决策树/Markov混合模型,以模拟每一种筛查策略的长期效用,包括临床和成本效益终点。该模型假设水平跨度为50年时间,其成本效益折损率为3.5%。采用敏感度分析以验证模型假设及确定哪种模型数据输入对结果的影响最大。
结果 与不筛查策略比较,筛查2型糖尿病、筛查糖尿病和糖耐量受损并予以生活方式干预、筛查糖尿病和糖耐量受损并予以药物干预治疗3种策略,每获得一个质量调整生命年(QALY)估计所需成本分别为(每年成本效益贴现率为3.5%)14150英镑(17560欧元;27860美元)、6242英镑和7023英镑。在支付意愿阈值为20000英镑时,各主动筛查策略通过干预产生的成本效益概率分别为49%,93%和85%。
结论 对年龄45岁、高于平均风险水平的人群进行2型糖尿病和糖耐量受损的筛查,并对糖耐量受损人群予以适当的干预似乎具有很好的成本效益。单独筛查糖尿病而不对糖耐量受损人群予以干预的策略具有的成本效益仍不确定,需要进一步研究确定早期检测糖尿病的作用。 | Clare L Gillies Paul C Lambert Keith R Abrams Alex J Sutton Nicola J Cooper Ron T Hsu Melanie J Davies Kamlesh Khunti 张晓梅(译) 纪立农(校) | 2008 | 英国医学杂志中文版2008，11，5： | 1 |
| 11 | Identification of genes potentially involved in rupture of human atherosclerotic plaques显示文摘 | Birgit C G F Kitty B J M C Ron L J N | 2001 | Circ Res2001，89，6： | 1 |
| 12 | Trade Intensity and Business Cycle Sychronization:are Developing Countries Any Different 显示文摘 | Caldéron C Chong A Stein E | | 0，，01： | 1 |
| 13 | Proteomic analysis of dendritic cell-derived exosomes:a secreted subcellular compartment distinct from apoptotic vesicles显示文摘 | Théry C Boussac M Véron P | 2001 | J Immunol2001，166，12： | 1 |
| 14 | Age/size and time - specific effects of Schistosoma mansoni on energy allocation patterns of its snail host Bi omphalaria glabrata显示文摘 | | 1997 | Oecologia1997，24，4： | 1 |
| 15 | Phosphorus fractions in soil solution: influence of soil acidity and fertilizer additions显示文摘 | Ron Vaz M D Edwards A C Shand C A | 1993 | Plant and Soil1993，14，8： | 1 |
| 16 | Important literature on the use of adjonit variational methods and the Kalman filter in meteorology显示文摘 | Courtier P Derber J C Ron Errico | 1993 | Tellus1993，45，： | 1 |
| 17 | Classifying technological knowledge for presentation to mechanical engineering designers 显示文摘 | Ron C J Broens A M de Vries M J | 2003 | Design Studies2003，24，5： | 1 |
| 18 | The elusive yellow camellia:results from breeding and pigment analysis显示文摘 | Parks C R Scogin Ron | 1987 | American Camellia Yearbook1987，，： | 1 |
| 19 | Enumeration of thermophilic bacillus species in composts and identification with a random amplification polymorphic DNA RAPD protocol 显示文摘 | Yunqiao C Zhang Ron S Ronimus Nicola Turner | 2002 | System Appl Microibol2002，25，4： | 1 |
| 20 | Does national culture influence consumer's evaluation of travel services? a test of hofstede's model of cross-cultural differences 显示文摘 | Crotts J C Ron E | 2000 | Managing Service Quality2000，，6： | 1 |
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