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| 1 | Alterations of β-catenin and Tcf-4 instead of GSK-3β contribute to activation of Wnt pathway in hepatocellular carcinoma显示文摘Objective The goal of this study is to investigate the inappropriate activation of Wnt pathway in the hepatocarcinogenesis. Methods We analyzed the alterations of three key components of Wnt pathway, β-catenin, glycogen synthase kinase 3β (GSK-3β) and T cell factor 4 (Tcf-4), in 34 samples of hepatocellular carcinoma (HCC) and paracancerous normal liver by immunohistochemistry, polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP), direct sequencing, semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR) and in situ hybridization. Results We found 61.8% (21/34) of all the HCCs examined showed an abnormal β-catenin protein accumulation in the cytoplasm or nuclei. RT-PCR-SSCP and direct sequencing showed that β-catenin exon 3 mutations existed in 44.1% (15/34) of the HCCs. No mutations of GSK-3β or Tcf-4 were detected in HCCs. Moreover, mRNA of β-catenin and Tcf-4 but not GSK-3β was found to be over expressed in HCCs. On analyzing the relationship between alterations of β-catenin or Tcf-4 and C-myc or Cyclin D1 expression, we found that the mutations of β-catenin as well as over expression of β-catenin or Tcf-4 gene were independently correlated with C-myc gene over expression in HCCs. Conclusions Our present findings strongly suggest mutations of β-catenin as well as over expression of β-catenin and Tcf-4 gene activate the Wnt pathway in HCC independently with the target gene most likely to be C-myc. | 崔健 周信达 刘银坤 汤钊猷 Mahmoud Romeih | 2003 | Chinese Medical Journal2003，，12： | 9 |
| 2 | Immunization against Egyptian Schistosoma mansoni infection by multivalent DNA vaccine显示文摘由几抗原组成的多价的疫苗的发展是对不同寄生虫紧张和寄生虫生命周期阶段创造宽广范围的保护的一条新奇途径。我们以前证实了血吸虫 Sm21.7 和 Sm Fimbrin (SmFim ) 蛋白质能在老鼠导致保护。因此,这研究试图构造多价的 DNA 疫苗的 Sm21.7-SmFim/pBudCE4.1 并且评估它的有免疫力的功效。二血吸虫 mansoni 基因, Sm21.7 和 SmFim 的开的读物框架,被插入到为独立人士设计的真核细胞的表示原生质标志 pBudCE4.1 在哺乳动物的房间的二基因的表示。评估在里面在老鼠的多价的 Sm21.7-SmFim/pBudCE4.1 DNA 疫苗和它的免疫学的效果的 vitro 表示, recombinant 原生质标志 Sm21.7-SmFim/pBudCE4.1 习惯于 mRNA 的 transfect 293T 房间,和表示,蛋白质用反向的抄写聚合酶链反应和蛋白质印迹分析被检验。然后 Sm21.7-SmFim/pBudCE4.1 到的能力免于 S。mansoni 挑战感染在老鼠的种痘以后根据蠕虫负担和鸡蛋减小率被分析。种牛痘的老鼠出现了保护(56%) 的重要水平,和在数字和尺寸的减少,和在细胞的侧面的变化, granulomas。分别地,在肝和肠的鸡蛋减小是 41.53% 和 55.63% 相对仅仅收到了空向量的老鼠同样坚定。除了在蠕虫生存能力的减小,蠕虫肥沃和鸡蛋孵化能力被观察在使免疫的组跟随挑战感染。结果证明 Sm21.7-SmFim/pBudCE4.1 能表示 Sm2 1.7 和 SmFim mRNA 和蛋白质。连接酶的免疫吸着剂试金和蛋白质印迹分析显示使免疫的老鼠对 Sm21.7-SmFim/pBudCE4.1 产生了特定的免疫球蛋白 G。这些结果与多价的 S 建议那种痘。mansoni DNA 疫苗(SmFim-Sm21.7/pBudCE4.1 ) 不仅在蠕虫和卵负担导致重要减小,而且显著地减少卵 granulomas 的尺寸。在摘要,多价的疫苗与保护的重要水平刺激了特定的免疫并且反病理学的效果。 | Mahmoud H Romeih Hanem M Hassan Tarek S Abou Shousha Mohamed A Saber | 2008 | Acta Biochimica et Biophysica Sinica2008，40，4： | 3 |
| 3 | Fetal responses to epidural analgesia as evi- denced by Doppler indices显示文摘 | Manaa EM Romeih MS | 2008 | Middle East J Anesthesiol2008，19，6： | 1 |
| 4 | Evaluation of cardiac function by magnetic resonance imaging during the follow-up of patients with Kawasaki disease 显示文摘 | Tacke CE Romeih S Kuipers IM | 2013 | Circ Cardiovasc Imaging2013，6，1： | 1 |
| 5 | Low-fat white-brined cheese made from bovine milk and two commercial fat mimetics: chemical, physical and sensory attributes显示文摘 | Romeih E A Michaelidou A Biliaderis C G | 2002 | International Dairy Journal2002，12，6： | 1 |
| 6 | Recovery of right and leftventricular function after acute pulmonary embolism显示文摘 | Klok FA Romeih S Kroft LJ | 2011 | ClinRadiol2011，66，： | 1 |
| 7 | Wnt signaling inhepatocelluar carcinoma: analysis of mutation and expression of betacatenin, T-cell factor-4 and glycogen synthase kinease 3-beta genes显示文摘 | Cui J Zhou X Liu Y Tang Z Romeih M | 2003 | J Gastroenterol Hepatol Hepatol2003，18，7： | 1 |
| 8 | Evaluation of cardiac function by magnetic resonance imaging during the follow-up of patients with Kawasaki disease显示文摘 | Tacke CE Romeih S Kuipers IM | 2013 | Circ Cardiovaso Imaging2013，6，1： | 1 |
| 9 | Fetal responses to epidural analgesia as evidenced by Doppler indices显示文摘 | Manaa EM Romeih MS | 2008 | Middle East J Anesthesiol2008，19，6： | 1 |
| 10 | Fetal responses to epidural analgesia as evidenced by Doppler indices 显示文摘 | Manaa EM Romeih MS | 2008 | Middle East J Anesthesiol2008，19，6： | 1 |
| 11 | Function of RARgamma and RARalpha2 at the initiation of retinoid signaling is essential for avian embryo survival and for distinct events in cardiac morphogenesis显示文摘 | Romeih M Cui J Michaille JJ | 2003 | Dev Dyn2003，228，4： | 1 |
| 12 | Recovery of right and left ventricular function after acute pulmonary embolism显示文摘 | F.A. Klok S. Romeih L.J.M. Kroft J.J.M. Westenberg M.V. Huisman A. de Roos | 2011 | Clinical Radiology2011，，12： | 1 |
| 13 | Synthesis and pharmacological testing of some new phenotloiazine devatives显示文摘 | Ei-Said M K EI-Moghazy Aly S M Romeih F F | 1991 | J Pharm Sic1991，32，12： | 1 |
| 14 | Evaluation of cardi ac function by magnetic resonance imaging during the follow up of patients with kawasaki disease 显示文摘 | Tacke CE Romeih S Kuipers IM | 2013 | Circ Cardiovasc Imaging2013，6，1： | 1 |
| 15 | Fetal responses to epidural analgesia as evi- denced by Doppler indices 显示文摘 | Manaa EM Romeih MS | 2008 | Middle East J Anesthesiol2008，19，6： | 1 |
| 16 | Recovery of fight and left ventricular function after acute pulmonary embolism显示文摘 | Klok FA Romeih S Kroft LJ | 2011 | Clin Radiol2011，66，12： | 1 |
| 17 | The Glenn Shunt Revisited, A Single Center Registry in Ain Shams University Cardiology Department显示文摘Background:Bidirectional Glenn shunts have long been available as palliative procedures for patients with single ventricle physiology that is,patients unsuitable for biventricular repair.In our country they are performed at an older age than that recommended by the literature.So,we aim to assess post bidirectional Glenn shunt patients to detect the presence of any complications and to evaluate their functional capacity.Methods:This was a descriptive study that included all patients who underwent a bidirectional Glenn shunt and were referred for follow up in Ain shams university hospitals.History taking including NYHA class and physical activity,clinical examination,six-minutes-walk test,laboratory investigations,full echocardiographic assessment were done for all patients.Some patients needed invasive cardiac catheterization.Results:Our registry included 178 patients who underwent bidirectional Glenn procedure referred for follow up in Ain Shams university hospital from January 2019 till July 2020.The mean age of the registry was 18.7±8.26 years(range between 5 and 37 years),85 males(48%)and 93 females(52%).Regarding the basic anatomy,double outlet right ventricle with hypoplastic left ventricle was the commonest.Furthermore,our descriptive study confirmed many characteristic similarities between our patients and patients in developing countries.Our patients underwent bidirectional Glenn shunt at a median age of 6 years which is considered a relatively old age but similar to other studies that have been made in developing countries like Pakistan,India and Iran.There is a significant delay in the operation in Egyptian patients due to lack of patients’awareness,few numbers of primary health care facilities and high economic burden.Conclusion:Although the presence of slight systemic desaturation,our study demonstrated a satisfactory functional capacity among our patients;thus,bidirectional Glenn can be considered an acceptable definite univentricular repair in patients with late presentation. | Mina Tewfik Maiy El-Sayed Alaa Roushdy Soha Romeih Dina Ezzeldin Hebatalla Attia | 2022 | Congenital Heart Disease2022，17，1： | 0 |
| 18 | Incidental radiological findings suggestive of COVID-19 in asymptomatic patients显示文摘Despite routine screening of patients for coronavirus disease 2019(COVID-19)symptoms and signs at hospital entrances,patients may slip between the cracks and be incidentally discovered to have lung findings that could indicate COVID-19 infection on imaging obtained for other reasons.Multiple case reports and case series have been published to identify the pattern of this highly infectious disease.This article addresses the radiographic findings in different imaging modalities that may be incidentally seen in asymptomatic patients who carry COVID-19.In general,findings of COVID-19 infection may appear in computed tomography(CT),magnetic resonance imaging,positron emission tomography-CT,ultrasound,or plain X-rays that show lung or only apical or basal cuts.The identification of these characteristics by radiologists and clinicians is crucial because this would help in the early recognition of cases so that a rapid treatment protocol can be established,the immediate isolation to reduce community transmission,and the organization of close monitoring.Thus,it is important to both the patient and the physician that these findings are highlighted and reported. | Marwa Romeih Mary R Mahrous Mohamed El Kassas | 2022 | World Journal of Radiology2022，14，1： | 0 |
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