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| 1 | Liver fat deposition and mitochondrial dysfunction in morbid obesity:An approach combining metabolomics with liver imaging and histology显示文摘AIM: To explore the usefulness of magnetic resonance imaging(MRI) and spectroscopy(MRS) for assessment of non-alcoholic fat liver disease(NAFLD) as compared with liver histological and metabolomics findings. METHODS: Patients undergoing bariatric surgery following procedures involved in laparoscopic sleeve gastrectomy were recruited as a model of obesityinduced NAFLD in an observational, prospective, singlesite, cross-sectional study with a pre-set duration of 1 year. Relevant data were obtained prospectively and surrogates for inflammation, oxidative stress and lipid and glucose metabolism were obtained through standard laboratory measurements. To provide reliable data from MRI and MRS, novel procedures were designed to limit sampling variability and other sources of error using a 1.5T Signa HDx scanner and protocols acquired from the 3D or 2D Fat SAT FIESTA prescription manager. We used our previously described 1H NMRbased metabolomics assays. Data were obtained immediately before surgery and after a 12-mo period including histology of the liver and measurement of metabolites. Values from 1H NMR spectra obtained after surgery were omitted due to technical limitations.RESULTS: MRI data showed excellent correlation with the concentration of liver triglycerides, other hepatic lipid components and the histological assessment, w h i c h e xc l u d e d t h e p r e s e n c e o f n o n-a l c o h o l i c steatohepatitis(NASH). MRI was sufficient to follow up NAFLD in obese patients undergoing bariatric surgery and data suggest usefulness in other clinical situations. The information provided by MRS replicated that obtained by MRI using the-CH3 peak(0.9 ppm), the-CH2- peak(1.3 ppm, mostly triglyceride) and the-CH=CH- peak(2.2 ppm). No patient depicted NASH. After surgery all patients significantly decreased their body weight and steatosis was virtually absent even in patients with previous severe disease. Improvement was also observed in the serum concentrations of selected variables. The most relevant findings using metabolomics indicate increased levels of triglyceride and monounsaturated fatty acids in severe steatosis but those results were accompanied by a significant depletion of diglycerides, polyunsaturated fatty acids, glucose-6-phosphate and the ATP/AMP ratio. Combined data indicated the coordinated action on mitochondrial fat oxidation and glucose transport activity and may support the consideration of NAFLD as a likely mitochondrial disease. This concept may helpto explain the dissociation between excess lipid storage in adipose tissue and NAFLD and may direct the search for plasma biomarkers and novel therapeutic strategies. A limitation of our study is that data were obtained in a relatively low number of patients.CONCLUSION: MRI is sufficient to stage NAFLD in obese patients and to assess the improvement after bariatric surgery. Other data were superfluous for this purpose. | Nahum Calvo Raúl Beltrán-Debón Esther Rodríguez-Gallego Anna Hernández-Aguilera Maria Guirro Roger Mariné-Casadó Lidón Millá Josep M Alegret Fàtima Sabench Daniel del Castillo María Vinaixa Miguelàngel Rodríguez Xavier Correig Roberto García-álvarez Javier A Menendez Jordi Camps Jorge Joven | 2015 | World Journal of Gastroenterology2015，21，24： | 2 |
| 2 | Catheter - related blood stream in - fection rates in a mixed - medical - surgical ICU population be- fore and after the implementation of a central line bundle 显示文摘 | Soni RA Rogers G Valenti A | 2008 | Chest2008，134，： | 1 |
| 3 | Impulse noise:theoretical solutions to the quandary of cochlear protection显示文摘 | Clifford RE Rogers RA | 2009 | Annals of Otolo- gy Rhinology ~ Laryngology2009，118，： | 1 |
| 4 | Relating the phagocytosis of microparticles by alveolar macrophages to surface chemistry: the effect of 1,2-dipalmitoylphosphatidylc holine显示文摘 | Evora C Soriano I Rogers RA | 1998 | J Controlled Release1998，51，23： | 1 |
| 5 | Impulse noise: theoretical solutions to the quandary of cochlear protection 显示文摘 | Clifford RE Rogers RA | 2009 | Ann Otol Rhinol Laryngol2009，118，6： | 1 |
| 6 | Cacheter-related blood stream re- fection rates in a mixed medical, sursical ICU population befare and af- ter the implementation of a central line bundle 显示文摘 | Soni RA Rogers G Valenti A | 2008 | Chest2008，134，4： | 1 |
| 7 | Evolutionary and biomedical insights from the rhesus macaque genome 显示文摘 | Gibbs RA Rogers J Katze MG | 2007 | Science2007，316，5822： | 1 |
| 8 | Catheter-relat- ed blood stream infection rates in a mixed medical-surgi- cal ICU population before and after the implementation of a central line bundle显示文摘 | SONI RA ROGERS G VALENTI A | 2008 | Chest2008，134，6： | 1 |
| 9 | Catheter-related blood stream infection rates in a mixed medical-surgical ICU population before and after the implementation of a central line bundle显示文摘 | Soni RA Rogers G Valenti A | 2008 | Chest2008，134，6： | 1 |
| 10 | Lipid and membrane protein transfer from human neutrophils to schistosomes is mediated by ligand binding显示文摘 | Rogers RA Jack RM Furlong ST | 1993 | J Cell Sci1993，106，2： | 1 |
| 11 | Catheter-related hlood stream infection rates in a mixed medical-surgical ICU popu- lation before and after the implementation of a central line bundle显示文摘 | Soni RA Rogers G Valenti A | 2008 | Chest2008，134，6： | 1 |
| 12 | Catheter- related blood stream infection (CRBS) rates in a mixed medical surgical ICU population before and after the implementation of a central line bundle (CLB) 显示文摘 | Soni RA Rogers G Valenti A | 2008 | Chest2008，134，4： | 1 |
| 13 | Relating the phagocytosis of microparticles by alveolar macrophages to surface chemistry : the effect of 1,2-dipalmitoylphosphatidylcholine 显示文摘 | EVORA C SORIANO I ROGERS RA | 1998 | J Control Release1998，51，23： | 1 |
| 14 | Case scenario:amniotic fluid embolism显示文摘 | Dean LS Rogers RP 3rd Harley RA | 2012 | Anesthesiology2012，116，1： | 1 |
| 15 | The effect of dialyzer reuse on dialysis delivery 显示文摘 | Sherman RA Cody RP Rogers ME | 1994 | Am J Kidney Dis1994，24，： | 1 |
| 16 | Impulse noise: theoretical solution to the quandry of colhlear protection 显示文摘 | Cifford RE Roger RA | 2009 | Ann Otol Rhinol Laryngol2009，118，6： | 1 |
| 17 | Circulating estradiol and osteoprotegerin as determinants of bone turnover and bone density in postmenopausal women 显示文摘 | Rogers A Saleh G Harnon RA | 2002 | J Clin Endocrinol Metab2002，86，10： | 1 |
| 18 | Pulsatile transdermal delivery of LHH using electroporation 显示文摘 | Riviere JE Monteiro-Rivere NA Rogers RA | 1995 | J Controlled Rellease1995，36，3： | 1 |
| 19 | Catheter-related blood stream infec- tion rates in a mixed medical-surgical ICU population before and after the implementation of a central line bundle 显示文摘 | Soni RA Rogers G Valenti A | 2008 | Chest2008，134，： | 1 |
| 20 | Catheter - related blood streaminfection rates in a mixed medical - surgical ICU population before and after the implementation of a central line bundle 显示文摘 | Soni RA Rogers G Valenti A | 2008 | Chest2008，134，4： | 1 |
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