2篇
您的检索式：作者名="Resit Demir"
|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Identification of differentially expressed genes after partial rat liver ischemia/reperfusion by suppression subtractive hybridization显示文摘AIM: To identify potential diagnostic target genes in early reperfusion periods following warm liver ischemia before irreversible liver damage occurs.METHODS: We used two strategies (SSH suppression subtractive hybridization and hybridization of cDNA arrays)to determine early changes in gene expression profiles in a rat model of partial WI/R, comparing postischemic and adjacent nonischemic liver lobes. Differential gene expression was verified (WT/R; 1 h/2 h) and analyzed in more detail after warm ischemia (1 h) in a reperfusion time kinetics (0, 1, 2 and 6 h) and compared to untreated livers by Northern blot hybridizations. Protein expression was examined on Western blots and by immunohistochemistry for four differentially expressed target genes (Hsp70,Hsp27, Gadd45a and IL-1rl).RESULTS: Thirty-two individual WI/R target genes showing altered RNA levels after confirmation by Northern blot analyzes were identified. Among them, six functionally uncharacteristic expressed sequences and 26 known genes (12 induced in postischemic liver lobes, 14 with higher transcriptional expression in adjacent nonischemic liver lobes). Functional categories of the verified marker genes indicate on the one hand cellular stress and tissue damage but otherwise activation of protective cellular reactions (AP-1 transcription factors, apoptosis related genes, heat shock genes). In order to assign the transcriptional status to the biological relevant protein level we demonstrated that Hsp70, Hsp27, Gadd45a and IL-1rI were clearly up-regulated comparing postischemic and untreated rat livers, suggesting their involvement in the WI/R context.CONCLUSION: This study unveils a WI/R response gene set that will help to explore molecular pathways involved in the tissue damage after WI/R. In addition, these genes especially Hsp70and Gadd45a might represent promising new candidates indicating WI/R liver damage. | Christine Fallsehr Christina Zapletal Michael Kremer Resit Demir Magnus von Knebel Doeberitz Ernst Klar | 2005 | World Journal of Gastroenterology2005，11，9： | 4 |
| 2 | Hypoxia generates a more invasive phenotype of turnout cells: an in vivo experimental setup base- don the chorioallantoic membrane显示文摘 | Resit Demir Lisa Naschberger Ilknur Demir | 2009 | Pathol Oneol Res2009，15，3： | 1 |
您所在的机构暂未开通此部分的功能，请联系管理员