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| 1 | Engineering infectious foot-and-mouth disease virus in vivo from a full-length genomic cDNA clone of the A/AKT/58 strain显示文摘Two full-length genomic cDNA clones, pTA/FMDV and pCA/FMDV, were constructed that contained three point-mutants [A174G and A308G (not present in pTA/FMDV); T1029G] in the genome compared with the wild type A/AKT/58 strain of foot-and-mouth disease virus. These two viruses were rescued by co-transfection of pCA/FMDV with pCT7RNAP, which can express T7 RNA polymerase in BHK-21 cell-lines, or by transfection of the in vitro transcribed RNA. Their biological properties were analyzed for their antigenicity, virulence in suckling-mice (LD50) and growth kinetics in BHK-21 cells. The in vivo rescued viruses showed high pathogenicity for 3-day-old unweaned mice (LD50=10?7.5). However, the in vitro transcribed RNA derived from pTA/FMDV had lower pathogenicity for suckling-mice (LD50=10?6), and the in vivo transcribed RNA recovered from pCA/FMDV co-transfected with pCT7RNAP showed no significant differences from the wild type virus. These data showed that recovery of the infectious foot-and-mouth disease virus directly from the use of in vivo techniques was better than from in vitro methods. Furthermore, the reverse genetic procedure technique was simplified to a faster one-step procedure based on co-transfection with pCT7RNAP. These results suggest that in vivo RNA tran- scripts may be more valuable for engineering recombinant foot-and-mouth disease virus than in vitro RNA transcripts, and may contribute to further understanding of the biological properties, such as replication, maturation and quasispecies, of the foot-and-mouth disease virus. | BAI XingWen1, LI PingHua1, CAO YiMei1, LI Dong1, LU ZengJun1, GUO JianHong1, SUN DeHui1, ZHENG HaiXue2, SUN Pu1, LIU XiangTao1, LUO JianXun1 & LIU ZaiXin1 1 Key Laboratory of Animal Virology of Ministry of Agriculture, National Foot-and-Mouth Disease Reference Laboratory of China, State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute of Chinese Academy of Agricultural Sciences, Lanzhou 730046, China 2 Veterinary Research Institute, Guangdong Agricultural Academy of Sciences, Guangzhou 510640, China | 2009 | Science China(Life Sciences)2009，52，2： | 6 |
| 2 | Photooxidation of hydrochlorofluocarbons and hydrofluorocarbons initiated by OH radicals显示文摘PhotooxidationofhydrochlorofluocarbonsandhydrofluorocarbonsinitiatedbyOHradicals￥ZhongJinxian;MuYujing;YangWenxiang(ResearchC... | Zhong Jinxian Mu Yujing Yang Wenxiang(Research Center for Eco-Environmental Sciences Chinese Academy of Sciences, Beijing 100085, China)Chen Dazhou(Chinese Center for Certified Reference Materials, Beijing 100013, China) | 1996 | Journal of Environmental Sciences1996，8，2： | 2 |
| 3 | WHO Child Growth Standards based on length/height, weight and age显示文摘 | WHO multicentre growth reference study group | 2006 | Acta Pediatriea2006，64，2： | 1 |
| 4 | Assessment of differences in linear growth among populations in the WHO Multicentre Growth Reference Study显示文摘 | WHO Multicentre Growth Reference Study Group | 2006 | Acta Paediatr2006，，： | 1 |
| 5 | WHO child growth standards based on length/height, weight and age显示文摘 | WHO Multicentre Growth Reference Study Group | 2006 | ActaPaediatr2006，，： | 1 |
| 6 | WHO Motor Development Study: windows of achievement for six gross motor development milestones 显示文摘 | WHO Multicentre Growth Reference Study Group | 2006 | Acta Paedi-atr Suppl2006，450，： | 1 |
| 7 | Complentary feeding in the WHO Multicentre Crowth Reference Study显示文摘 | WHO Muhicentre Crowth Reference Study Croup | 2006 | Acta Paediatr Suppl2006，450，： | 1 |
| 8 | Improved methods and assumptions for estimation of the HIV/ AIDS epidemic and its impact: Recommendations of the UNAIDS Reference Group on Estimates, Modelling and Projections 显示文摘 | The UNAIDS Reference Group on Estimates Modelling and Projec- tions | 2002 | AIDS2002，16，： | 1 |
| 9 | Enrolment and baseline characteristics in the WHO muhicentre growth reference study显示文摘 | WHO Muhicentre Growth Reference Study Group | 2006 | Acta Pediatr2006，，： | 1 |
| 10 | Improvef methods and assumptions for estimation of the HIV/AIDS epidemic and its impact:recommendation of the UNAIDS Reference Group on Estimates,Modeling and Projections显示文摘 | The UNAIDS Reference Group on Estimates Modeling and Projections | 2002 | AIDS2002，16，9： | 1 |
| 11 | A reference model of computer integrated manufacturing from the viewpoint of industrial automation显示文摘 | CIM Reference Model Committee | | Int J Computer Integrated Manufacturing0，2，2： | 1 |
| 12 | American Thoracic Society显示文摘 | Lung function testing:selection of reference values and interpretative strategies | 1991 | Am Rev Respir Dis1991，144，5： | 1 |
| 13 | WHO child growth standards based on length/height, weight and age 显示文摘 | WHO Multicentre Growth Reference Study Group | 2006 | Acta Paediatr Suppl2006，450，： | 1 |
| 14 | WHO Child Growth Standards based on length/height, weight and age显示文摘 | WHO Multicentre Growth Reference Study Group | 2006 | Acta Paediatrica2006，450，： | 1 |
| 15 | Improved methods and assumptions for estimation of the HIV/AIDS epidemic and its impact : Recommendations of the UNAIDS Reference Group on Estimates, Modelling and Projections 显示文摘 | UNAIDS Reference Group on Estimates Modeling and Projections | 2002 | AIDS2002，16，： | 1 |
| 16 | Improved methods and assumptions for estimation of the HIV/ AIDS epidemic and its impact: recommendations of the UNAIDS reference group on estimates, modelling and projections 显示文摘 | The UNAIDS Reference Group on Estimates Modelling and Projections | 2002 | MDS2002，16，9： | 1 |
| 17 | A dimple method for purification of C5a from citrated plasmal 显示文摘 | Refer L Frank M M Hanner C H | 1982 | J Immunol Methods1982，2753，1： | 1 |
| 18 | EnergyPlus Engineering Reference显示文摘 | The Reference to EnergyPlus Calculations DOE U S | 2010 | The Reference to En ergyPlus Calculations2010，，： | 1 |
| 19 | 显示文摘 | Guma MK Abdeldaim KS Per O et aL Toward a quantitative DNA- based definition of pneumococcal pneumonia: a comparison of Streptococcus pneumoniae target genes with special reference to the Spn9802 fragment | 2008 | Diagn Microbiol Infect Dis2008，60，： | 1 |
| 20 | Determinants of pulse wave velocity in healthy people and in the presence of cardiovascular risk factors:'establishing normal and reference values'显示文摘 | Reference Values for Arterial Stiffness'Collaboration | 2010 | Eur Heart J2010，31，19： | 1 |
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