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198篇 您的检索式:作者名="REMY L"
    题名 作者 年代 出处 被引量
1A Rapid and Cost-Effective Method for Genotyping Genome-Edited Animals:A Heteroduplex Mobility Assay Using Microfluidic Capillary Electrophoresis显示文摘The recent emergence and application of engineered endonucleases have led to the development of genome editing tools capable of rapidly implementing various targeted genome editions in a wide range of species.Moreover,these novel tools have become easier to use and have resulted in a great increase of applications.Whilst gene knockout(KO) or knockin(KI) animal models are relatively easy to achieve,there is a bottleneck in the detection and analysis of these mutations.Although several methods exist to detect these targeted mutations,we developed a heteroduplex mobility assay on an automated microfluidic capillary electrophoresis system named HMA-CE in order to accelerate the genotyping process.The HMA-CE method uses a simple PCR amplification of genomic DNA(gDNA) followed by an automated capillary electrophoresis step which reveals a heteroduplexes(HD) signature for each mutation.This allows efficient discrimination of wild-type and genome-edited animals down to the single base pair level.Vanessa Chenouard Lucas Brusselle Jean-Marie Heslan Séverine Remy Séverine Ménoret Claire Usal Laure-Hélène Ouisse Tuan Huy NGuyen Ignacio Anegon Laurent Tesson 2016Journal of Genetics and Genomics2016,43,5:4
2Dynamic kinetic asymmetric transformation of diene monoepoxides: a practical asymmetric synthesis of vinylglycinol, vigabatrin, and ethambutol 显示文摘 Richard C B Remy C L 2000J Am Chem Soc2000,122,:1
3Central pulmonary thromboembolism:diagnosis with spiral volumetric CT with the single-breathhold technique comparison with pulmonary angiography显示文摘Remy-Jardin M Remy J Wettinne L 1992Radiology1992,185,:1
4Polypeptide composition of light-harvesting complexes from some brown algae and diatoms显示文摘Caron L Remy R Berkaloff C 1988FEBS Lett1988,229,1:1
5Influence of austenite predeforma-tion on mechanical properties and strain-induced martensitic transformations of a high manganese steel显示文摘Sipos K Remy L Pineau A 0,,05:1
6Twinning and strain-induced FCC→ HCP transformation in the Fe-Mn-Cr-C system显示文摘Remy L Pineau A 0,,01:1
7AAISafeR limits ventricular pacing in unselected patients 显示文摘Guy P Gerard L Remi N 2007PACE2007,30,:1
8Central pulmonary thromboembolism:diagnosis with spiral volumetric CT with the single - breath hold technique - comparison with pulmonary angiography显示文摘Remy-Jardin M Remy J Wattine L 1992Radiology1992,185,2:1
9Suppression of fertility in male mice by immunization against LH receptor显示文摘REMY J J BOZON V COUTURE L 1993J Reprod Immunol1993,25,1:1
10Ridge-line optimal detector显示文摘Pierre G Hayet L Remi K 0,,:1
11Fatigue failure probability in a powder metallurgy Ni-base superalloy显示文摘Grison J Remy L 1997Engineering Fracture Mechanics1997,57,1:1
12Behavior of nickel-base superal- loy single crystals under thermomechanical fatigue 显示文摘FLEURY E REMY L 1994Metallurgical and Materials Transactions1994,25,:1
13Hemorrhagic colitis associated with a rare Escherichia coli serotype显示文摘RILEY L W REMIS R S HELGERSON S D 1983New England Journal of Medicine1983,308,12:1
14Influence of orientation on the low cycle fatigue of MAR - M 200 single crys- tals at 650 ~C II : Cyclic stress-strain behavior显示文摘CHIERAGAT'I'I R REMY L 1991Ma- terials Science and Engineering1991,141,:1
15Influence of orientation on the low cycle fatigue of MARM200 single crystals at 650 {2 显示文摘Chieragatti R Remy L 1991Ma- terial Science and Engineering A1991,141,:1
16Central pulmonary thromboembdism:diagnosis with spiral volumetric CT with single-breath-hold technique-comparison with pulmonary angiography显示文摘Remy-Jardin M Remy J Wattinne L 1992Radiology1992,185,:1
17High temperature low cycle fatigue and thermal-mechanical fatigue behaviour of an oxide-dispersion-strengthened nickel-base superal- loy 显示文摘VASSEUR E REMY L 1994Materials Science Engineering1994,184,:1
18Influence of orientation on the low cycle fatigue of MAR - M 200 single crys- tals at 650 ℃ I : Fatigue life behavior 显示文摘CHIERAGATI'I R REMY L 1991Materials Science and Engineering1991,141,:1
19Central pulmonary thomboembolism:diagnosis with spiral volumetric CT with the single -breath-hold technique:comparison with pulmonary angiography显示文摘Remy-Jardin M Remy J Wattinne L 1992Radiology1992,185,2:1
20Treatement of hemotysis by embolization of bronchial arteries显示文摘Remy J Arnaue L Fardol L 1977Radiology1977,122,:1
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