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| 1 | Delivery of surface-mediated non-viral gene nanoparticles from ultrathin layer-by-layer multilayers显示文摘An efficient and safe gene delivery system remains a challenge in the development of gene therapy.Polycation-based gene nanoparticles are a typical non-viral gene delivery system,which are able to transfect cells in vitro and in vivo.This paper reported a facile method for constructing biodegradable multilayers via layer-by-layer self-assembly,in which the polycation-based gene nanoparticles were loaded.Through this surface-mediated delivery system,adherent cells on the multilayer could be transfected in situ.Gene nanoparticles-loaded multilayers transfect cells with higher efficiency than naked DNA-loaded multilayers because of the complex configuration of the DNA.DNA nanoparticles/PGA multilayers constructed on the scaffold surface could also realize in situ transfection on the adherent cells.The well-structured,easy-processed multilayers may provide a novel approach to precisely controlled delivery of gene nanoparticles,which may have potential applications for gene therapy in tissue engineering and medical implants. | WANG XueFei,REN KeFeng,LIN QuanKui & JI Jian Key Laboratory of Macromolecule Synthesis and Functionalization,Ministry of Education Department of Polymer Science and Engineering,Zhejiang University,Hangzhou 310027,China | 2010 | Science China Chemistry2010，53，3： | 4 |
| 2 | MULTIPLE ELECTRONIC CONTROL UNITS CALIBRATION SYSTEM BASED ON EXPLICIT CALIBRATION PROTOCOL AND J1939 PROTOCOL显示文摘The rising number of electronic control units (ECUs) in vehicles and the decreasing time to market have led to the need for advanced methods of calibration. A multi-ECU calibration system was developed based on the explicit calibration protocol (XCP) and J1939 communication protocol to satisfy the need of calibrating multiple ECUs simultaneously. The messages in the controller area network (CAN) are defined in the J1939 protocol. Each CAN node can get its own calibration messages and information from other ECUs,and block other messages by qualifying the CAN messages with priority,source or destination address. The data field of the calibration message is designed with the XCP ,with CAN acting as the transport layer. The calibration sessions are setup with the event-triggered XCP driver in the master node and the responding XCP driver in the slave nodes. Mirroring calibration variables from ROM to RAM enables the user to calibrate ECUs online. The application example shows that the multi-ECU calibration system can calibrate multiple ECUs simultaneously,and the main program can also accomplish its calculation and send commands to the actuators in time. By the multi-ECU calibration system,the calibration effort and time can be reduced and the variables in ECU can get a better match with the variables of other ECUs. | ZHU Keqing XU Quankui YANG Lin ZHUO Bin | 2008 | Chinese Journal of Mechanical Engineering2008，21，1： | 3 |
| 3 | Augmented cellular uptake and homologous targeting of exosome-based drug loaded IOL for posterior capsular opacification prevention and biosafety improvement显示文摘Posterior capsular opacification(PCO),the most common complication after cataract surgery,is caused by the proliferation,migration and differentiation of residual lens epithelial cells(LECs)on the surface of the intraocular lens(IOL).Although drug-loaded IOLs have been successfully developed,the PCO prevention efficacy is still limited due to the lack of targeting and low bioavailability.In this investigation,an exosome-functionalized drug-loaded IOL was successfully developed for effective PCO prevention utilizing the homologous targeting and high biocompatibility of exosome.The exosomes derived from LECs were collected to load the anti-proliferative drug doxorubicin(Dox)through electroporation and then immobilized on the aminated IOLs surface through electrostatic interaction.In vitro experiments showed that significantly improved cellular uptake of Dox@Exos by LECs was achieved due to the targeting ability of exosome,compared with free Dox,thus resulting in superior anti-proliferation effect.In vivo animal investigations indicated that Dox@Exos-IOLs effectively inhibited the development of PCO and showed excellent intraocular biocompatibility.We believe that this work will provide a targeting strategy for PCO prevention through exosome-functionalized IOL. | Siqing Zhu Huiying Huang Dong Liu Shimin Wen Liangliang Shen Quankui Lin | 2022 | Bioactive Materials2022，7，9： | 1 |
| 4 | In Situ Endothelialization of Intravascular Stents Coated with an Anti-CD34 Antibody Functionalized Heparin-collagen Multilayer显示文摘 | Lin Quankui Xin Ding Qiu Fuyu | 2010 | Biomaterials2010，31，： | 1 |
| 5 | Regenerative cerium oxide nanozymes alleviate oxidative stress for efficient dry eye disease treatment显示文摘Dry eye disease(DED)is the most common eye disease in ophthalmic consultation except for refractive errors.Therefore,an exploration of valid and alternative therapeutic interventions is essential to feed the urgent medical need.It has been demonstrated that oxidative stress causes multiple adverse effects in the pathogenesis of DED,thence alleviating oxidative stress is an effective therapeutic strategy for the DED treatment.Herein,we developed a cerium oxide nanozyme combined with branched poly(ethylene imine)-graftpoly(ethylene glycol)(bPEI-g-PEG).Owing to its stable hydrophilic chains on the surface reducing the cytotoxicity and loads of amines groups that be combined with cerium ions through coordination bonds,the modified nanozymes(referred to as CNP@bPEI-g-PEG)are water soluble and highly biocompatible.Meanwhile,due to its excellent antioxidant activity,CNP@bPEI-g-PEG nanozymes can mimic the activity of superoxide dismutase and catalase to scavenge intracellular reactive oxygen species(ROS).Experimental studies firmly demonstrated that the modified nanozymes were auto-regenerative and more active in scavenging excessive ROS and alleviating oxidative stress by cerium-element valence state recycling,recovering the morphology of corneal,conjunctival epithelium and the number of goblet cells.The advanced combination may offer a superior therapeutic strategy to deal with oxidative stress for effective treatment of DED. | Haoyu Zou Haiting Wang Baoqi Xu Lin Liang Liangliang Shen Quankui Lin | 2022 | Regenerative Biomaterials2022，9，1： | 0 |
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