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您的检索式：作者名="Qitong Weng"
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| 1 | Guiding T lymphopoiesis from pluripotent stem cells by defined transcription factors显示文摘Achievement of immunocompetent and therapeutic T lymphopoiesis from pluripotent stem cells(PSCs)is a central aim in T cell regenerative medicine.To date,preferentially reconstituting T lymphopoiesis in vivo from PSCs remains a practical challenge.Here we documented that synergistic and transient expression of Runx1 and Hoxa9 restricted in the time window of endothelial-to-hematopoietic transition and hematopoietic maturation stages in a PSC differentiation scheme(iR9-PSC)in vitro induced preferential generation of engraftable hematopoietic progenitors capable of homing to thymus and developing into mature T cells in primary and secondary immunodeficient recipients.Single-cell transcriptome and functional analyses illustrated the cellular trajectory of T lineage induction from PSCs,unveiling the T-lineage specification determined at as early as hemogenic endothelial cell stage and identifying the bona fide pre-thymic progenitors.The induced T cells distributed normally in central and peripheral lymphoid organs and exhibited abundant TCRαβrepertoire.The regenerative T lymphopoiesis restored immune surveillance in immunodeficient mice.Furthermore,gene-edited iR9-PSCs produced tumor-specific T cells in vivo that effectively eradicated tumor cells.This study provides insight into universal generation of functional and therapeutic T cells from the unlimited and editable PSC source. | Rongqun Guo Fangxiao Hu Qitong Weng Cui Lv Hongling Wu Lijuan Liu Zongcheng Li Yang Zeng Zhijie Bai Mengyun Zhang Yuting Liu Xiaofei Liu Chengxiang Xia Tongjie Wang Peiqing Zhou Kaitao Wang Yong Dong Yuxuan Luo Xiangzhong Zhang Yuxian Guan Yang Geng Juan Du Yangqiu Li Yu Lan Jiekai Chen Bing Liu Jinyong Wang | 2020 | Cell Research2020，30，1： | 7 |
| 2 | Senescent bone marrow microenvironment promotes Nras-mutant leukemia显示文摘Dear Editor,In recent years,there is growing interest regarding the roles of senescent bone marrow(BM)microenvironment in the initiation of leukemia.Aged mice transplanted with AML1-ETO(AML1-ETO fusion protein)-positive hematopoietic stem cells(HSCs)present with a significant increase in the frequency of AMLETO-positive early progenitor cells in BM as well as an in crease of immature myeloid cells compared to young recipients(Vas et aL,2012).BM mesenchymal stem cells(MSCs)from myelodysplastic syndromes(MDS)animal models and MDS patients exhibit impaired proliferation and differentiation potentials,abnormal cytoki ne secretion,and dysregulated gene expression profile(Lopez-Villar et al.,2009;Geyh et al.,2013;Mattiucci et al.,2018).However,the causal relationship between senes・cent BM microenvironment and leukemia development is unclear.Whether senes・cent BM microenvironment initiates or accelerates leukemia development remains unknown. | Peiqing Zhou Chengxiang Xia Tongjie Wang Yong Dong Qitong Weng Xiaofei Liu Yang Geng Jinyong Wang Juan Du | 2021 | Journal of Molecular Cell Biology2021，13，1： | 1 |
| 3 | Regeneration of immunocompetent B lymphopoiesis from pluripotent stem cells guided by transcription factors显示文摘Regeneration of functional B lymphopoiesis from pluripotent stem cells(PSCs)is challenging,and reliable methods have not been developed.Here,we unveiled the guiding role of three essential factors,Lhx2,Hoxa9,and Runx1,the simultaneous expression of which preferentially drives B lineage fate commitment and in vivo B lymphopoiesis using PSCs as a cell source.In the presence of Lhx2,Hoxa9,and Runx1 expression,PSC-derived induced hematopoietic progenitors(iHPCs)immediately gave rise to pro/pre-B cells in recipient bone marrow,which were able to further differentiate into entire B cell lineages,including innate B-1a,B-1b,and marginal zone B cells,as well as adaptive follicular B cells.In particular,the regenerative B cells produced adaptive humoral immune responses,sustained antigen-specific antibody production,and formed immune memory in response to antigen challenges.The regenerative B cells showed natural B cell development patterns of immunoglobulin chain switching and hypermutation via cross-talk with host T follicular helper cells,which eventually formed T cell-dependent humoral responses.This study exhibits de novo evidence that B lymphopoiesis can be regenerated from PSCs via an HSC-independent approach,which provides insights into treating B cell-related deficiencies using PSCs as an unlimited cell resource. | Qi Zhang Bingyan Wu Qitong Weng Fangxiao Hu Yunqing Lin Chengxiang Xia Huan Peng Yao Wang Xiaofei Liu Lijuan Liu Jiapin Xiong Yang Geng Yalan Zhao Mengyun Zhang Juan Du Jinyong Wang | 2022 | Cellular & Molecular Immunology2022，19，4： | 0 |
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