| 1 | Expression of survivin and its significance in colorectal cancer显示文摘AIM: To study the expression of survivin,a novel memberof inhibitors of apoptosis protein (IAP) and its significancein colorectal carcinoma.METHODS: Survivin mRNA expression was evaluated bysemi-quantitative reverse transcriptase polymerase chainreaction (RT-PCR) in 52 colorectal carcinoma samples and48 adjacent normal colorectal tissue samples. PCR productwas sequenced to verify the desired result. Expressions ofsurvivin protein, proliferating cell nuclear antigen labellingindex (PI) and apoptotic index (AI) were detectedimmunohistochemically in 52 human colorectal carcinomas.RESULTS: The expression of survivin mRNA was detectedin a significantly greater proportion of colorectal carcinomasamples than in adjacent normal colorectal tissues (67.3%vs 25%; P<0.01). There was no relationship betweensurvivin mRNA expression in colorectal carcinomas andsex, tumor size, histological types, lymphnode metastasis,distant metastasis and Dukes' stage. The PCR productshared 99% of homology with human counterparts. Survivinexpression was observed immunohistochemically in 27 of52 cases of colorectal carcinoma (51.9%). The AI wassignificently lower in survivin positive group than in survivinnegative group (0.67+0.18% vs 1.14+0.42%; P<0.001),while the PI was greater in survivin positive group than insurvivin negative group (51+22% vs 27+18%, P<0.001).CONCLUSION: Survivin is a special tumor marker independentof histopathological characteristics. It may play an importantrole during human colorectal tumorigenesis by inhibitingapoptosis and accelerating proliferative activity of colorectaltumor cells. | Wei-ChangChen QiangLiu Jian-XinFu Su-YaKang | 2004 | World Journal of Gastroenterology2004，10，19： | 15 |
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