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您的检索式：作者名="Qian Peter Su"
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| 1 | Dynamic tubulation of mitochondria drives mitochondrial network formation显示文摘 | Chong Wang Wanqing Du Qian Peter Su Mingli Zhu Peiyuan Feng Ying Li Yichen Zhou Na Mi Yueyao Zhu Dong Jiang Senyan Zhang Zerui Zhang Yujie Sun Li Yu | 2015 | Cell Research2015，25，10： | 8 |
| 2 | 驱动蛋白介导自噬性溶酶体出管显示文摘本研究通过在体外重构自噬性溶酶体出管的单分子研究体系,详细解析了驱动蛋白KIF5B介导自噬性溶酶体出管再生的分子机制。 | Wanqing Du Qian Peter Su Yang Chen Yueyao Zhu Dong Jiang Yueguang Rong Senyan Zhang Yixiao Zhang He Ren Chuanmao Zhang Xinquan Wang Ning Gao Yanfeng Wang Lingfei Sun 孙育杰 俞立 | 2017 | 科学新闻2017，19，4： | 0 |
| 3 | PN-ImTLSM facilitates high-throughput low background single-molecule localization microscopy deep in the cell显示文摘When imaging the nucleus structure of a cell,the out-of-focus fluorescence acts as background and hinders the detection of weak signals.Light-sheet fluorescence microscopy(LSFM)is a wide-field imaging approach which has the best of both background removal and imaging speed.However,the commonly adopted orthogonal excitation/detection scheme is hard to be applied to single-cell imaging due to steric hindrance.For LSFMs capable of high spatiotemporal single-cell imaging,the complex instrument design and operation largely limit their throughput of data collection.Here,we propose an approach for high-throughput background-free fluorescence imaging of single cells facilitated by the Im-mersion Tilted Light Sheet Microscopy(ImTLSM).ImTLSM is based on a light-sheet projected off the optical axis of a water immersion objective.With the illumination objective and the detection objective placed opposingly,ImTLSM can rapidly patrol and optically section multiple individual cells while maintaining single-molecule detection sensitivity and resolution.Further,the simplicity and robustness of ImTLSM in operation and maintenance enables high-throughput image collection to establish background removal datasets for deep learning.Using a deep learning model to train the mapping from epi-illumination images to ImTLSM illumination images,namely PN-ImTLSM,we demonstrated crossmodality fluorescence imaging,transforming the epi-illumination image to approach the background removal performance obtained with ImTLSM.We demonstrated that PN-ImTLSM can be generalized to large-field homogeneous illumination imaging,thereby further improving the imaging throughput.In addition,compared to commonly used background removal methods,PN-ImTLSM showed much better performance for areas where the background intensity changes sharply in space,facilitating high-density single-molecule localization microscopy.In summary,PN-ImTLSM paves the way for background-free fluorescence imaging on ordinary inverted microscopes. | Boxin Xue Caiwei Zhou YizhiQin Yongzheng Li Yuao Sun Lei Chang Shipeng Shao Yongliang Li Mengling Zhang Chaoying Sun Renxi He Qian Peter Su Yujie Sun | 2021 | Biophysics Reports2021，7，4： | 0 |
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