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| 1 | 3D cellular visualization of intact mouse tooth using optical clearing without decalcification显示文摘Dental pulp is composed of nerves,blood vessels,and various types of cells and surrounded by a thick and hard enamel-dentin matrix.Due to its importance in the maintenance of tooth vitality,there have been intensive efforts to analyze the complex cellularlevel organization of the dental pulp in teeth.Although conventional histologic analysis has provided microscopic images of the dental pulp,3-dimensional (3D) cellular-level visualization of the whole dental pulp in an intact tooth has remained a technically challenging task.This is mainly due to the inevitable disruption and loss of microscopic structural features during the process of mechanical sectioning required for the preparation of the tooth sample for histological observation.To accomplish 3D microscopic observation of thick intact tissue,various optical clearing techniques have been developed mostly for soft tissue,and their application for hard tissues such as bone and teeth has only recently started to be investigated.In this work,we established a simple and rapid optical clearing technique for intact mouse teeth without the time-consuming process of decalcification.We achieved 3D cellular-level visualization of the microvasculature and various immune cell distributions in the whole dental pulp of mouse teeth under normal and pathologic conditions.This technique could be used to enable diverse research methods on tooth development and regeneration by providing 3D visualization of various pulpal cells in intact mouse teeth. | Sujung Hong Jingu Lee Jin Man Kim Sun-Young Kim Hyung-Ryong Kim Pilhan Kim | 2019 | International Journal of Oral Science2019，11，3： | 1 |
| 2 | Flat ampli- tude equal spacing 798-channel Rayleigh-assisted Bril- louinlRaman multiwavelength comb generation in disper- sion compensating fiber 显示文摘 | Min Bumki Kim Pilhan Park Namkyoo | 2001 | IEEE Photonics Technology Letters2001，13，12： | 1 |
| 3 | Interaction of tetraspan(in) TM4SF5 with CD44 promotes self‐renewal and circulating capacities of hepatocarcinoma cells显示文摘 | Doohyung Lee Juri Na Jihye Ryu Hye‐Jin Kim Seo Hee Nam Minkyung Kang Jae Woo Jung Mi‐Sook Lee Haeng Eun Song Jungeun Choi Gyu‐Ho Lee Tai Young Kim June‐Key Chung Ki Hun Park Sung‐Hak Kim Hyunggee Kim Howon Seo Pilhan Kim Hyewon Youn Jung Weon Lee | 2015 | Hepatology2015，，6： | 1 |
| 4 | SNR enhancement of OTDR using biorthogonal codes and generalized inverses显示文摘 | DUCKEY L HOSUNG Y PILHAN K | 2005 | IEEE Photonics Technology Letters2005，17，10： | 1 |
| 5 | SNR Enhancement of OTDR Using Biorthogonal Codes and Generalized Inverses显示文摘 | LEE DUCKEY YOON HOSUNG KIM PILHAN | 2005 | IEEE photonics technology letters2005，17，1： | 1 |
| 6 | Handheld endomicroscope using a fiber-optic harmonograph enables real-time and in vivo confocal imaging of living cell morphology and capillary perfusion显示文摘Confocal laser endomicroscopy provides high potential for noninvasive and in vivo optical biopsy at the cellular level.Here,we report a fully packaged handheld confocal endomicroscopic system for real-time,high-resolution,and in vivo cellular imaging using a Lissajous scanning fiber-optic harmonograph.The endomicroscopic system features an endomicroscopic probe with a fiber-optic harmonograph,a confocal microscope unit,and an image signal processor.The fiber-optic harmonograph contains a single mode fiber coupled with a quadrupole piezoelectric tube,which resonantly scans both axes at~1 kHz to obtain a Lissajous pattern.The fiber-optic harmonograph was fully packaged into an endomicroscopic probe with an objective lens.The endomicroscopic probe was hygienically packaged for waterproofing and disinfection of medical instruments within a 2.6-mm outer diameter stainless tube capable of being inserted through the working channel of a clinical endoscope.The probe was further combined with the confocal microscope unit for indocyanine green imaging and the image signal processor for high frame rate and high density Lissajous scanning.The signal processing unit delivers driving signals for probe actuation and reconstructs confocal images using the auto phase matching process of Lissajous fiber scanners.The confocal endomicroscopic system was used to successfully obtain human in vitro fluorescent images and real-time ex vivo and in vivo fluorescent images of the living cell morphology and capillary perfusion inside a single mouse. | Kyungmin Hwang Yeong-Hyeon Seo Daniel YKim Jinhyo Ahn Soyoung Lee Kyung Hee Han Koun-Hee Lee Sangyong Jon Pilhan Kim Kate EYu Hyungsin Kim Shin-Hyuk Kang Ki-Hun Jeong | 2020 | Microsystems & Nanoengineering2020，6，1： | 0 |
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