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| 1 | Potential role of human papilloma virus in the pathogenesis of gastric cancer显示文摘AIM:To demonstrate the presence and biological activity of human papilloma virus(HPV)in gastric cancer(GAC)tissues.METHODS:The study involved 84 surgically treated patients with gastric adenocarcinoma,regardless of the clinical stage of the disease.The presence of HPV DNA of high oncogenic risk types in formalin-fixed,paraffinembedded tumor samples was determined using quantitative polymerase chain reaction analysis.A stringentprotocol of prevention of cross-and environmental contamination was applied during DNA isolation,and amplification,as well as confirmation of the biological activity of the virus in tumor cells,was implemented.The study utilized the Real-time High Risk HPV test,which detects the DNA of 14 HPV subtypes that are considered to have high oncogenic potential.The overexpression of the p16INK4a protein assessed immunohistochemically was considered confirmation of the HPV infection.RESULTS:Among the 89 patients initially included in the study group,diagnostic results were obtained for84 individuals.In five cases,either the histopathological material was too scant to isolate the necessary amount of DNA,or the isolated DNA was significantly degraded,resulting in the failure of internal control amplification within the predefined number of 35 cycles.Those patients were excluded from further analysis.The amplification of HPV DNA was demonstrated in none of the84 tissue samples;thus,all cases were considered to have a negative DNA status of highly oncogenic HPV subtypes.Immunohistochemical staining provided diagnostic results for all of the examined tissue samples,and excluded the accumulation of the p16INK4a protein in tumor cells,thus confirming the lack of active HPV infection in all of the individuals.CONCLUSION:The study does not confirm the presence or biological activity of HPV in tumor tissues.Thus,the relationship between GAC and HPV infection,in the Central European population seems doubtful. | Miroslaw Snietura Dariusz Waniczek Wojciech Piglowski Agnieszka Kopec Ewa Nowakowska-Zajdel Zbigniew Lorenc Malgorzata Muc-Wierzgon | 2014 | World Journal of Gastroenterology2014，20，21： | 2 |
| 2 | Influence of plasma modification on biological properties of poly (ethylene terephthalate)显示文摘 | PIGLOWSKI J GANCARZ I STANISZEWSKA-KUS J | 1994 | Biomaterials1994，15，11： | 1 |
| 3 | The structural and functional analysis of the human HSPA2 gene promoter re- gion显示文摘 | Piglowski W Nowak R Krawczyk Z | 2007 | Acta Biochim Pol2007，54，1： | 1 |
| 4 | The HspA2 protein localizes in nucleoli and centrosomes of heat shocked cancer cells显示文摘 | Scieglinska D Piglowski W Mazurek A | 2008 | J Cell Bioehem2008，104，6： | 1 |
| 5 | Crystallization in modified blends of polyamide and polypropylene 显示文摘 | Piglowski J Gancarz I Wlazlak M | 2000 | Polymer2000，41，： | 1 |
| 6 | Influence of plasma modification on biological properties of poly(ethylene terephthalate)显示文摘 | Piglowski J Gancarz I Staniszewska-Kus J | 1994 | Biomaterials1994，15，11： | 1 |
| 7 | Influence of plasma modification on biological properties of poly ( ethylene terephthalate ) 显示文摘 | Piglowski J Gancarz J | 1994 | Biomaterials1994，15，11： | 1 |
| 8 | The structural and functional analysis of the human HSPA2 gene promoter region 显示文摘 | PIGLOWSKI W NOWAK R KRAWCZYK Z | 2007 | ActaBiochimPol2007，54，1： | 1 |
| 9 | Influence of plasma modification on biological properties of poly ( ethylene terephthalate) 显示文摘 | Piglowski J Ganearz J | 1994 | Biomaterials1994，15，11： | 1 |
| 10 | Oxazline-functionalized Hydrogenated Nitrile Rubber as Impact Modifier for Polyamide-6显示文摘 | PigLowski J Gancarz I WlaZlak W | 2000 | Polymer2000，，41： | 1 |
| 11 | Crystallization in modified blends of polyamide and polypropylene显示文摘 | Piglowski J Gancarz I Wlazlak M | 2000 | Polym2000，41，： | 1 |
| 12 | The structural and functional analysis of the human HSPA2 gene promoter region显示文摘 | Piglowski W Nowak R Krawczyk Z | 2007 | Acta Biochim Pol2007，54，1： | 1 |
| 13 | Spontaneous exfoliation and self-assembly phenomena in polyvinylpyrrolidone/synthetic layered silicate nanocomposites显示文摘 | M. Zabska K. Jaskiewicz A. Kiersnowski K. Szustakiewicz S. Rathgeber J. Piglowski | 2011 | Radiation Physics and Chemistry2011，，10： | 1 |
| 14 | Influence of plasma modification on biological properties of polyethylene terephthalate显示文摘 | PIGLOWSKI J GANCARZA I STANISZWSKA K J | 1994 | Biomaterials1994，15，11： | 1 |
| 15 | Adhesion in polyamide/compatibilizer/polypropylene systems显示文摘 | TRELINSKA W MARIA | 1999 | Journal of Macromolecular Science Physics Sep-Nov1999，38，56： | 1 |
| 16 | Oxazolinefunctionalized hydrogenated nitfile rubber as impact modifier for polyamide-6显示文摘 | PIGLOWSKI J GANCARZ I WLAZLAK M | 2000 | Polymer2000，41，： | 1 |
| 17 | Influence of plasma modification on biologieal properties of poly ( ethylene terephthalate) 显示文摘 | PIGLOWSKI J GANEARZ 1 STAN1SZEWSKA K J | 1994 | Biomaterials1994，15，11： | 1 |
| 18 | Differen- tial expression of HSPA1 and HSPA2 proteins in human tissues; tissue microarray-based immunohistochemical study 显示文摘 | SCIEGLINSKA D PIGLOWSKI W CHEKAN M | 2011 | Histochem Cell Biol2011，135，： | 1 |
| 19 | HSPA2 overexpression protects V79 fibroblasts against borte- zomib-induced apoptosis 显示文摘 | FILIPCZAK PT PIGLOWSKI W GLOWALA-KOSINSKA M | 2012 | Biochem Cell Biol2012，90，： | 1 |
| 20 | Influence of plasma modification on biological properties of poly (ethylene terephthalate) 显示文摘 | Piglowski J Gancarz I Staniszewska-Kus J | 1994 | Biomaterials1994，15，11： | 1 |
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