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您的检索式：作者名="Pierre Germon"
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| 1 | Rck of Salmonella enterica, subspecies enterica serovar Enteritidis, mediates Zipper-like internalization显示文摘沙门氏菌能通过它的类型 III 分泌物系统(T3SS-1 ) 入侵非吞噬细胞的房间,它导致一个扳机入口过程。这研究显示出那沙门氏菌 enterica,亚种 enterica serovar Enteritidis 能也经由 Rck 外部膜蛋白质入侵房间。Rck 必要、足够启用非侵略的 E。coli 和 Rck 涂的祷告遵守并且入侵不同房间。与不同 Rck 肽涂的乳胶祷告的成为主观分析证明包含氨基酸 140-150 的肽支持了粘附,而在 150 和 159 之间的氨基酸调制了侵略。主导否定的衍生物的表示和特定的禁止者的使用在激活 Arp2/3 建筑群触发充满肌动朊的累积的形成表明了小 GTPases Rac1 和 Cdc42 的关键角色,导致 Rck 依赖的成为主观。最后,扫描并且有 Rck 涂的祷告和 E 的传播电子显微镜学。表示 Rck 的 coli 揭示了形成了像拉链的结构的象 microvillus 一样延期,吞没支持者祷告和细菌。总的来说,我们的结果提供新卓见进沙门氏菌 T3SS 独立的侵略机制并且强烈建议 Rck 导致像拉链的入口机制。因而,沙门氏菌似乎是发现能导致拉链和扳机机制入侵宿主细胞的第一个细菌。 | Manon Rosselin Isabelle Virlogeux-Payant Christian Roy Elisabeth Bottreau Pierre-Yves Sizaret Lily Mijouin Pierre Germon Emmanuelle Caron Philippe Velge Agnes Wiedemann | 2010 | Cell Research2010，20，6： | 4 |
| 2 | Punch‑excised explants of bovine mammary gland to model early immune response to infection显示文摘Background Mammary gland(MG)infections(mastitis)are frequent diseases of dairy cows that affect milk quality,animal welfare and farming profitability.These infections are commonly associated with the bacteria Escherichia coli and Staphylococcus aureus.Different in vitro models have been used to investigate the early response of the MG to bacteria,but the role of the teat in mastitis pathogenesis has received less attention.In this study,we used punch-excised teat tissue as an ex vivo model to study the immune mechanisms that arise early during infection when bacteria have entered the MG.Results Cytotoxicity and microscopic analyses showed that bovine teat sinus explants have their morphology and viability preserved after 24 h of culture and respond to ex vivo stimulation with TLR-agonists and bacteria.LPS and E.coli trigger stronger inflammatory response in teat when compared to LTA and S.aureus,leading to a higher produc-tion of IL-6 and IL-8,as well as to an up-regulation of proinflammatory genes.We also demonstrated that our ex vivo model can be applied to frozen-stored explants.Conclusions In compliance with the 3Rs principle(replacement,reduction and refinement)in animal experimenta-tion,ex vivo explant analyses proved to be a simple and affordable approach to study MG immune response to infec-tion.This model,which better reproduces organ complexity than epithelial cell cultures or tissue slices,lends itself particularly well to studying the early phases of the MG immune response to infection. | Pablo Gomes Noleto Florence B.Gilbert Christelle Rossignol Patricia Cunha Pierre Germon Pascal Rainard Rodrigo Prado Martins | 2023 | Journal of Animal Science and Biotechnology2023，14，6： | 0 |
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