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| 1 | A BAC clone of MDV strain GX0101 with REV-LTR integration retained its pathogenicity显示文摘The complete genome of Marek's disease virus (MDV) strain GX0101, which was integrated with the LTR sequences of REV, was cloned in Escherichia coli as a bacterial artificial chromosome (BAC). BAC vector sequences were introduced into the US2 locus of the MDV genome by homologous recombination. The viral DNA containing the BAC vector was used to transform Escherichia coli strain of DH10B. Then the recombinant virus was successfully rescued by transfection of the recombinant BAC DNA into primary chicken embryo fibroblast (CEF). This BAC viral clone was named bac-GX0101. When the reconstituted virus was inoculated into 1-day-old birds, visceral tumors could be detected as early as 62 d post infection. There was no difference in growth ability and pathogenicity to birds between the BAC derived virus and its parental virus. The BAC derived virus maintained its oncogenicity and immunosuppressive effects. In conclusion, the complete genome of GX0101 strain was successfully cloned into BAC and the infectious clone was rescued. With the powerful BAC manipulation system, the infectious clone will provide a useful tool for further understanding the functional roles of the inserted REV-LTR sequence in the GX0101 strain of MDV. | SUN AiJun LAWRENCE Petherbridge ZHAO YuGuang LI YanPeng NAIR Venugopal K CUI ZhiZhong | 2009 | Chinese Science Bulletin2009，54，15： | 15 |
| 2 | 带有REV-LTR片段的MDV野毒株GX0101 BAC克隆的构建及拯救病毒的致病性分析显示文摘将整合有禽网状内皮组织增生症病毒(reticuloendotheliosis,REV)长末端重复序列(LTR)的马立克氏病病毒(命名为GX0101)全基因组作为细菌人工染色体(bacterial artificial chromosome,BAC)克隆进大肠杆菌.BAC载体通过同源重组插入MDV基因组的US2区,含BAC载体的MDV DNA电转化大肠杆菌菌株DH10B,鉴定含MDV全基因组的BAC克隆(MDV BAC),提取BAC DNA,转染原代鸡胚成纤维细胞(CEF),成功拯救出了重组病毒,命名为bac-GX0101.将此病毒腹腔接种1日龄鸡,在攻毒后62d开始检测到内脏肿瘤,动物实验结果表明,bac-GX0101毒株与亲本GX0101毒株对机体的致病性等方面的影响没有差异,bac-GX0101毒株仍然保留着很好的免疫抑制功能及致肿瘤能力.该感染性克隆bac-GX0101为研究该重组野毒株中REV-LTR插入片段及其他基因的生物学功能提供了有用的技术平台. | 孙爱军 PETHERBRIDGE Lawrence ZHAO YuGuang 李延鹏 NAIR Venugopal K 崔治中 | 2009 | 科学通报2009，54，11： | 7 |
| 3 | Sulfur doping of diamond films:Spectroscopic, electronic, and gas-phase studies 显示文摘 | JAMES R PETHERBRIDGE PAUL W MAY GARETH M FUGE | 2002 | Journal of Applied Physics2002，91，6： | 1 |
| 4 | Replication-Compe- tent bacterial Artificial Chromosomes of Marek's Disease Virus: Novel Tools for generation of molecularly defined herpesvirus Vaccines 显示文摘 | Petherbridge L Howes K Susan J B | 2003 | J Virol2003，77，16： | 1 |
| 5 | Simu- lation of H C S Containing Gas Mixtures Relevant to Di- amond Chemical Vapour Deposition 显示文摘 | Petherbridge JR May PW Shallcross DE etal | 2003 | Diamond Related Materials2003，12，3： | 1 |
| 6 | Discomfort glare and the lighting of buildings显示文摘 | PETHERBRIDGE P HOPKINSON R G | 1950 | London: Transaction of Illuminating Engineering Society1950，，39： | 1 |
| 7 | Absolute quantita- tion of marek's disease virus genome copy number in chicken feather and lymphocyte samples using real- time PCR显示文摘 | Baigent SJ Petherbridge LJ Howes K | 2005 | Journal of virological methods2005，123，1： | 1 |
| 8 | The viral envelope is a major determinant for the induction of lymphoid and myeloid tumours by avian leukosis virus subgroups A and J respectively显示文摘 | CHESTERS P M HOWES K PETHERBRIDGE L | 2002 | J Gen Virol2002，83，： | 1 |
| 9 | The viral envelope is a major determinant for the induction of lymphoid and myeloid tumours by avian leukosis virus subgroups A and J, respectively 显示文摘 | Chesters P M Howes K Petherbridge L | 2002 | J Gen Virol2002，83，10： | 1 |
| 10 | Absolute quantitation of Marek's disease virus genome copy number in chicken feather and lymphocyte samples using real-time PCR显示文摘 | Baigent S J Petherbridge L J Howes K | | 0，，1： | 1 |
| 11 | Herpesvirus of turkey reconstituted from bacterial artificial chromosome clones induces protection against Marek's disease 显示文摘 | Susan J Baigent Lawrence J Petherbridge Lorraine P Smith | 2006 | J Gen Virol2006，87，： | 1 |
| 12 | Differential quantification of cloned CVI988 vaccine strain and virulent RB-1B strain of Marek's disease viruses in chicken tissues,using real-time PCR显示文摘 | Baigent S J Smith L P Petherbridge L J | | 0，，1： | 1 |
| 13 | Herpesvirus of turkey reconstituted from bacterial artificial chromosome clones induces protection against Marek's disease显示文摘 | Petherbridge L J Smith L P | 2006 | J Gen Virol2006，87，： | 1 |
| 14 | In Situ Plasma Diagnostics of the Chemistry Behind Sulfur Doping of CVD Diamond Films 显示文摘 | Petherbridge J R May P W Fuge G M | 2002 | Diam Relat Mater2002，11，3： | 1 |
| 15 | Unravelling aspects of the gas phase chemistry involved in diamond chemical vapour deposition显示文摘 | ASHFOLD M N R MAY P W PETHERBRIDGE J R ROSSER K N SMITH J A MANKELEVICH Y A SUETIN N V | 2001 | Physical Chemistry Chemical Physics2001，3，17： | 1 |
| 16 | Sulfur Doping of Diamond Films: Epectroscopic, Electronic and Gas-phase Studies 显示文摘 | Petherbridge J R May P W Fuge G M | 2002 | J Appl Phys2002，91，6： | 1 |
| 17 | Comparative fulllength sequence analysis of oncogenic and vaccine(Rispens)strains of Marek's disease vims显示文摘 | Petherbridge L J Zhao Y | 2007 | J Gen Virol2007，88，： | 1 |
| 18 | Optical emission spect roscopic studies of microwave enhanced diamond CVD using CH4/CO2plasmas显示文摘 | Elliott M A May P W Petherbridge J | 2000 | Diamond and Related Materia ls2000，9，： | 1 |
| 19 | Absolute quantitation of Marek's disease virus genome copy number in chicken feather and lymphocyte samples using real-time PCR显示文摘 | Baigent S J Petherbridge L J Howes K | 2005 | Virol Methods2005，123，1： | 1 |
| 20 | Cloning of gallid herpesvirus 3 ( marek ' s disease virus serotype - 2 ) genome as infectious bacterial artificial chromosomes for analysis of viral gene functions 显示文摘 | Petherbridge L Xu H Zhao Y | 2009 | J Virol Methods2009，158，： | 1 |
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