1篇
您的检索式：作者名="Peter T.Nelson"
|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Expression of miR-15/107 Family MicroRNAs in Human Tissues and Cultured Rat Brain Cells显示文摘The miR-15/107 family comprises a group of 10 paralogous microRNAs(miRNAs),sharing a 50AGCAGC sequence.These miRNAs have overlapping targets.In order to characterize the expression of miR-15/107 family miRNAs,we employed customized TaqMan Low-Density micro-fluid PCR-array to investigate the expression of miR-15/107 family members,and other selected miRNAs,in 11 human tissues obtained at autopsy including the cerebral cortex,frontal cortex,primary visual cortex,thalamus,heart,lung,liver,kidney,spleen,stomach and skeletal muscle.miR-103,miR-195 and miR-497 were expressed at similar levels across various tissues,whereas miR-107 is enriched in brain samples.We also examined the expression patterns of evolutionarily conserved miR-15/107 miRNAs in three distinct primary rat brain cell preparations(enriched for cortical neurons,astrocytes and microglia,respectively).In primary cultures of rat brain cells,several members of the miR-15/107 family are enriched in neurons compared to other cell types in the central nervous system(CNS).In addition to mature miRNAs,we also examined the expression of precursors(pri-miRNAs).Our data suggested a generally poor correlation between the expression of mature miRNAs and their precursors.In summary,we provide a detailed study of the tissue and cell type-specific expression profile of this highly expressed and phylogenetically conserved family of miRNA genes. | Wang-Xia Wang Robert J.Danaher Craig S.Miller Joseph R.Berger Vega G.Nubia Bernard S.Wilfred Janna H.Neltner Christopher M.Norris Peter T.Nelson | 2014 | Genomics, Proteomics & Bioinformatics2014，12，1： | 9 |
您所在的机构暂未开通此部分的功能，请联系管理员