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| 1 | The effectiveness of differ- ent modalities for the rehabilitation of unilateral neglect in stroke patients: A systematic review显示文摘 | Pernet L Jughters A Kerekhofs E | 2014 | NeuroRehabilitation2014，35，7： | 1 |
| 2 | Mechanism of inhibition of DNA gyrase by analogs of nalidixic acid:The target of the drugs is DNA显示文摘 | Shen L L Pernet A G | 1985 | Proc Nalt Acad Sci USA1985，82，2： | 1 |
| 3 | Mechanism of inhibition of DNA gyrase by analogues of nalidixic acid: the target of the drugs is DNA显示文摘 | SHEN L L PERNET G A | 1985 | Proc Natl Acad Sci USA1985，82，： | 1 |
| 4 | AB027.Nogo-A neutralisation improves vision recovery after retinal injury显示文摘Background:In glaucoma and after an ischemic injury of the retina,excessive activation of N-Methyl-D-Aspartate receptors,a type of glutamatergic receptors,induces the death of retinal ganglion cells and an irreversible vision loss.The painless loss of retinal cells does not allow for a swift diagnostic and treatment of retinal damages.There is no efficient therapy to improve retinal functions in this case.In order to develop new therapeutic approaches for retinal injury,we propose,in this study,to stimulate neuronal plasticity of the visual system by neutralising the glial protein,Nogo-A.The inhibitory action of this protein on axonal regeneration is well known in spinal cord injuries but not in the visual system.We thus studied the function of Nogo-A in vision recovery in mice.Methods:Nogo-A activities were chronically blocked by deleting its gene in KO mice and acutely,by intravitreal injections of an antibody known as 11C7.Inner retina lesions were done by injection of 0.5 or 5 nmol of NMDA in the vitreous humor.A PBS buffer was administered in control animals.The visual system functions were accessed with an optokinetic test in awake mice,by electroretinography(ERG)in the eye and visual evoked potentials in the visual cortex.Cell survival of retinal ganglion cells,amacrine and bipolar cells was evaluated on histological sections by immunofluorescence.Changes in expression of Nogo-A,its receptors and neuronal plasticity associated molecules were observed by Western blot and q-PCR.Results:At NMDA doses of≤0.5 nmol,Nogo-A KO mice show a recovery of optokinetic responses much faster than in WT mice.Surprisingly,a single injection of the antibody,11C7 was sufficient to improve VEPs of NMDA injured animals as compared to control antibody.Furthermore,ERGs showed that a dose of 0.5 nmol induced retinal lesions limited to the ganglion cell layer,with significant changes to the VEPs but without influencing photoreceptors and inner nuclear layer cells functions.However,5 nmol of NMDA affected the survival of inner nuclear layer cells and reduced by~50%their activity.Conclusions:Our results show that the neutralisation of Nogo-A can improve visual functions after injury to the inner retina.Inhibition of Nogo-A could thus be an efficient way to treat pathologies like glaucoma. | Julius Baya Mdzomba Sandrine Joly Léa Rodriguez Frédéric Bretzner Vincent Pernet | 2018 | Annals of Eye Science2018，，1： | 0 |
| 5 | AB043.The glial protein Nogo-A is necessary to maintain retinal structure and function in physiological conditions显示文摘Background:Our previous studies revealed that Nogo-A gene ablation improved visual function recovery after retinal injury.Moreover,Nogo-A expression is highly expressed in the healthy retina.Its physiological role in retinal function is not known.The purpose of this current study was to determine the effects of acute Nogo-A silencing on retinal neuron structure and function in physiological conditions.Methods:Nogo-A silencing was done by intravitreal injection of adeno-associated virus serotype 2.2 containing a short hairpin RNA sequence(AAV2.2 shRNA-Nogo-A)and a GFP reporter gene in adult C57BL/6J mice.As control,an empty AAV2.2 vector was used.Infection of retinal cells was followed by fluorescent fundoscopy.Changes in Nogo-A expression were analysed by Western blotting in whole retinal lysates.Electroretinography was used to monitor retinal activity.The assessment of optokinetic reflex(OKR)allowed to follow visual acuity in unrestrained mice.Immunofluorescence on histological sections using the following cell markers,i.e.,RNA-binding protein with multiple splicing(RBPMS)and sex-determining region Y-box 2(Sox-2)allowed to visualize retinal ganglion cells(RGCs)and Müller glia respectively.Results:GFP fluorescence revealed efficient AAV2.2 transfection in the ganglion cell layer and the inner nuclear layer 30 days after viral injection.By Western blotting,Nogo-A expression was decreased by~75%in AAV2.2-shRNA-Nogo-A-treated retinae(n=3)as compared to the control mice(n=3).Strikingly,AAV2.2-shRNA-Nogo-A-injected animals(n=10)had a visual acuity reduction of 43.7%as compared to control(n=7),60 days after transfection.Electroretinography(ERG)b-wave and a-wave amplitudes were also decreased by~35%and 24.4%respectively relative to controls.After two months of transfection,RBPMS-positive RGCs were reduced by~30%in AAV2.2-shRNA-Nogo-A(n=4)compared to non-injected contralateral eyes(n=4).The number of Sox2-expressing Müller cells was not affected after Nogo-A knockdown.Conclusions:Nogo-A gene silencing in the retina has deleterious effects on the mouse retinal structure and function,suggesting an important role for Nogo-A in retinal physiology. | Julius Baya Mdzomba Léa Rodriguez Sandrine Joly Vincent Pernet | 2019 | Annals of Eye Science2019，，1： | 0 |
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