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| 1 | 海藻糖快速检测方法的建立与初步应用显示文摘海藻糖是生物体合成的、具有抗逆保护作用的天然二糖,在食品加工与贮藏、微生物发酵过程具有重要作用。该文采用前期获得的专一性海藻糖酶,建立了一种定量测定海藻糖的快速方法。该方法对海藻糖的检测限为0.019 mg/m L,定量限为0.1 mg/m L;海藻糖的线性检测范围为0.1 mg/m L^150.0 mg/m L,回收率为98.80%~101.33%。该方法操作简便快捷、特异性高、重复性好,在菌种改良与食品原料营养筛查等海藻糖快速定量检测中具有良好的应用前景。 | 苗佳 王彩喆 牛丹丹 Nokuthula Peace Mchunu 田康明 Suren Singh Kugenthiren Permaul 王正祥 | 2019 | 食品与发酵工业2019，45，23： | 5 |
| 2 | 疏绵状嗜热丝孢菌外切β-葡聚糖酶的基因克隆与酶学特征显示文摘【目的】疏绵状嗜热丝孢菌是一种嗜热丝状真菌,具有合成与分泌多种耐热酶的能力,从中找寻酶活高、耐热性能优良的β-葡聚糖酶。【方法】对疏绵状嗜热丝孢菌其中一个外切β-葡聚糖酶的编码基因gln B进行克隆,并在毕赤酵母GS115中表达。【结果】在摇瓶水平上重组菌的产酶活为11.5 U/m L,重组酶在SDS-PAGE中的大小约为48 k D。重组Gln B最适作用温度为65°C,最适作用p H为5.0;在低于50°C或p H 3.0-10.0之间具有良好稳定性。重组酶水解海带三糖,首先生成单糖和二糖,延长酶作用时间,可以进一步将其中的二糖部分水解为单糖。【结论】疏绵状嗜热丝孢菌来源的重组酶Gln B为外切β-葡聚糖酶,具有较好的耐热性能和p H稳定性。 | 王瑞杰 Nokuthula Peace Mchunu 牛丹丹 Kugenthiren Permaul Suren Singh 刘晓光 路福平 | 2016 | 微生物学通报2016，43，2： | 3 |
| 3 | Community-acquired antibiotic resistance in urinary i- solates from adult women in Canada显示文摘 | MCLSAAC W J MAZZULLI T PERMAUL J | 2006 | Can J Infect Dis Med Microbiol2006，17，： | 1 |
| 4 | Thermostable chitinase II from Thermomyces lanuginosus SSBP: Cloning, structure prediction and molecular dynamics simulations 显示文摘 | Khan F I Govender A Permaul K | 2015 | Journal of Theoretical Biology2015，374，： | 1 |
| 5 | Error-prone PCR of a fun-gal xylanase for improvement of its alkaline and thermal stability显示文摘 | Stephens DE Singh S Permaul K | | 0，，01： | 1 |
| 6 | Error-prone PCR of a fungal xylanase for improvement of its alkaline and thermal stability 显示文摘 | Stephens DE Singh S Permaul K | 2009 | FEMS Microbiol Lett2009，293，1： | 1 |
| 7 | Directed evolution of the thermostable xylanase from Thermomyces lanuginosus 显示文摘 | Stephens D E Rumbold K Permaul K | 2007 | Journal of Biotechnology2007，127，3： | 1 |
| 8 | Directed evolution of the thermostable xylanase from Therraomyces lanuginosus显示文摘 | Stephens D E Rumbold K Permaul K Prior B A Singh S | 2007 | J Biotechnol2007，127，3： | 1 |
| 9 | Xylanase superproducer: genome sequence of a compost-loving thermophilic fungus, Thermomyces lanuginosus strain SSBP显示文摘 | McHunu N P Permaul K Abdul Rahman A Y | 2013 | Genome Announcements2013，1，00： | 1 |
| 10 | Error-Prone PCR of a Fungal Xylanase for Improvement of Its Alkaline and Thermal Stability显示文摘 | D E Stephens S Singh K Permaul K | 2009 | FEMS Microbiol Lett2009，293，1： | 1 |
| 11 | Directed evolution of the thermostable xylanase from Thermomyces lanuginosus 显示文摘 | STEPHENS D E RUMBOLD K PERMAUL K | 2007 | Journal of Biotechnology2007，127，3： | 1 |
| 12 | Expression of an alkalo-tolerant fungal xylanase enhanced by directed evolution in Pichia pastoris and Escherichia coli显示文摘 | MCHUNU N P SINGH S PERMAUL K | 2009 | Journal of Biotechnology2009，141，12： | 1 |
| 13 | Production of !3 -xylanase by a Thermomyces lanuginosus MO 134 mutant on corn cobs and its application in biobleaching of bagasse pulp显示文摘 | Kumar K S Manimaran A Permaul K | 2009 | Journal of Bioscience and Bioengineering2009，107，5： | 1 |
| 14 | Production of β- xylanase by a Thermomyces lanuginosus MC 134 mutant on corn cobs and its application in hiobleaching of bagasse pulp 显示文摘 | Kumar K S Manimaran A Permaul K | 2009 | Journal of Bioscience and Bioengineering2009，107，5： | 1 |
| 15 | Xylanase superproducer: genome sequence of a compost-loving thermophilic fungus,Thermomyces lanuginosus strain SSBP显示文摘 | Mchunu NP Permaul K Abdul Rahman AY | 2013 | Genome Announcements2013，1，00： | 1 |
| 16 | Production of β-xylanase by a Thermomyces lanuginosus MC 134 mutant on corn cobs and its application in biobleaching of bagasse pulp显示文摘 | KUMAR K S MANIMARAN A PERMAUL K | 2009 | Journal of Bioscience and Bioengineering2009，107，5： | 1 |
| 17 | Reduction of patulin during apple juice clarification显示文摘 | Bissessur J Permaul K Odhav B | 2001 | Journal of Food Protection2001，，： | 1 |
| 18 | Amylase production in solid state fermentation by the thermophilic fungus Thermomyces lanuginosus显示文摘 | KUNAMNENI A PERMAUL K SINGH S | 2005 | Journal of Bioscience and Bioengineering2005，100，2： | 1 |
| 19 | Acupuncture blocks cold stress-induced increases in the hypothalamic-pituitary- adrenal axis in the rat显示文摘 | Eshkevari L Permaul E Mulroney SE | 2013 | J Endocrinol2013，，： | 1 |
| 20 | Hyper produc- tion of cellulase-free xylanase by Thermomyces lanuginosus SSBP on bagasse pulp and its application in biobleaching显示文摘 | MANIMARAN A KUMAR K S PERMAUL K | 2009 | Applied Microbiol- ogy and Biotechnology2009，81，5： | 1 |
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