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24篇 您的检索式:作者名="Pericles P"
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1Development of a quantum-dot-labelled magnetic immunoassay method for circulating colorectal cancer cell detection显示文摘AIM:To detect of colorectal cancer(CRC) circulating tumour cells(CTCs) surface antigens,we present an assay incorporating cadmium selenide quantum dots(QDs) in these paper.METHODS:The principle of the assay is the immunomagnetic separation of CTCs from body fluids in conjunction with QDs,using specific antibody biomarkers:epithelial cell adhesion molecule antibody,and monoclonal cytokeratin 19 antibody.The detection signal was acquired from the fluorescence signal of QDs.For the evaluation of the performance,the method under study was used to isolate the human colon adenocarcinoma cell line(DLD-1) and CTCs from CRC patients' peripheral blood.RESULTS:The minimum detection limit of the assay was defined to 10 DLD-1 CRC cells/mL as fluorescence was measured with a spectrofluorometer.Fluorescenceactivated cell sorting analysis and Real Time RT-PCR,they both have also been used to evaluate the performance of the described method.In conclusion,we developed a simple,sensitive,efficient and of lower cost(than the existing ones) method for the detection of CRC CTCs in human samples.We have accomplished these results by using magnetic bead isolation and subsequent QD fluorescence detection.CONCLUSION:The method described here can be easily adjusted for any other protein target of either the CTC or the host.Maria Gazouli Anna Lyberopoulou Pericles Pericleous Spyros Rizos Gerassimos Aravantinos Nikolaos Nikiteas Nicholas P Anagnou Efstathios P Efstathopoulos 2012World Journal of Gastroenterology2012,18,32:6
2A natural extension of the conventional finite volume method in- to polygonal unstructured meshes for CFD application 显示文摘CHOW P CROSS M PERICLEOUS K 1996Applied Mathematical Modelling1996,20,2:1
3A Natural Extensionof the Conventional Finite Volume Method Into Polygonal Un-structured Meshes for CFD Application显示文摘CHOW P CROSS M PERICLEOUS K 1996Applied Mathemat-ical Modelling1996,20,2:1
4A nature extension of the conventional finite volume method into polygonal unstructured meshes for CFD application显示文摘Chow P Cross M Pericleous K 1996Applied Mathematical Modelling1996,20,2:1
5Mathematical modeling tools for the optimization of direct smelting processes显示文摘DAVIS M PERICLEOUS K CROSS M SCHWARZ P 1998Applied Mathematical Modeling1998,22,1:1
6Identification and validation of reference genes to study the gene expression in Gluconacetobacter diazotrophicus grown in different carbon sources using RT-qPCR显示文摘Galisa Pericles S da Silva Helder A P Macedo Aline V M 2012Journal of Microbiological Methods2012,91,1:1
7Evaluation of dis- tortions in laser welded shipbuilding parts using local-global finite element approach 显示文摘Tsirkas S A Papanikos P Pericleous K 2003Science and Technology of Welding and Joining2003,8,2:1
8A randomized comparison of native Escherichia coli asparaginase and polyethylene glycol conjugated asparaginase for treatment of children with newly diagnosed standard-risk acute lymphoblastic leukemia: a Children's Cancer Group study 显示文摘Avramis V I Sencer S Periclou A P 2002Blood2002,99,6:1
9A natural extension of the conventional finite volume method into polygonal un- structured meshes for CFD application 显示文摘Chow P Cross M Pericleous K 1996Applied Mathematical Modelling1996,20,2:1
10Evaluation of distortions in laser welded shipbuilding parts using local-global finite element approach显示文摘Tsirkas S A Papanikos P Pericleous K 2003Science and Technology of Welding and Joining2003,8,2:1
11A randomized comparison of native Escherichia coli asparaginase and polyethylene glycol conjugated asparaginase for treatment of children with newly diagnosed standard-risk a-cute lymphoblastic leukemia: A children,s cancer group study显示文摘Avramis V I Sencer S Periclou A P 2002Blood2002,99,6:1
12A ran- domized comparison of native Escherichia eoli asparaginase and polyethylene glycol conjugated asparaginase for treatment of children with newly diagnosed standard-risk acute lymphoblas- tie leukemia: a Children's Cancer Group study显示文摘AVRAMIS V I SENCER S PERICLOU A P 2002Blood2002,99,6:1
13Evaluation of distortions in laser welded shipbuildingparts using local-global finite element approach显示文摘TSIRKAS S A PAPANIKOS P PERICLEOUS K 2003Science and Technology of Welding and Joining2003,8,2:1
14A Nature Extension of the Conventional Finite Volume Method into Polyonal Unstructured Meshes for CFD Application显示文摘Chow P Cross M Pericleous K 1996Applied Mathematical Modelling1996,,20:1
15A natural extension of the conventional finite volume method into polygonal unstructured meshes for CFD application显示文摘Chow P Cross M Pericleous K 1996Applied Mathematical Modelling1996,20,2:1
16CD40- CD40L interactions provide 'third-party' costimulation for T cell response against B7-1-transfected human breast tumor cells显示文摘Pericle F Epling-Burnette P Podack E 1997J Leukoc Biol1997,61,2:1
17A natural extension of the conventional finite volume method into polygonal unstructured meshes for CFD application 显示文摘CHOW P CROSS M PERICLEOUS K 1996Applied Mathematical Modeling1996,20,:1
18A nature extension of the conventional finite volume method into polyonal unstructured meshes for CFD application 显示文摘Chow P Cross M Pericleous K 1996App Math Modelling1996,20,:1
19查看详情显示文摘Pericles P Maria G Anna L 0,,03:1
20Functional outcome and structural integrity following mini - open repair of large and massive rotator cuff tears : a 3 - 5 year follow - up study 显示文摘Pericles P Dimitrios K Achilleas B 2011J Shoulder El- bow Surg2011,20,1:1
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