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| 1 | Differential regulation of cadherin expression by osteotropic hormones and growth factors in vitro in human osteoprogenitor cells显示文摘Aim: To examine if cadherins are expressed constitutively in human bone marrow stromal cells (hBMSC) and investigate the regulation of cadherin expression by various osteotropic hormones and local factors. Methods: Cadherin expression was examined in first passaged (secondary) hBMSC as well as in the conditionallyimmortalized human osteoprogenitor cell line (hOP-7). Using a monoclonal antibody (MoAb C-1821) to a cytoplasmic domain common to all known cadherins (pan-cadherin MoAb), cadherins were immunolocalized in first passaged hBMSC as well as in hOP-7 cells. In addition, intense immunostaining for cadhedn expression was associated with alkaline phosphatase (ALP) in nodules formed in the high density cultures of hOP-7 cells. Human E-cadherin (HECD) was specifically detected by Western blotting in extracts of untreated hBMSC using an anti-HECD MoAb 004FD. Results: Differential regulation of cadherin expression by various osteotropic hormones and local factors (parathyroid hormone, dexamethasone,estradiol, prostaglandin E2, basic fibroblast growth factor, and tumor necrosis factor-β) was also observed. In addition, blocking cadherins with the MoAb C-1821 increased basal ALP activity and had an additive effect on 1, 25(OH)ED3-induced ALP activity. Conclusion: Cadherins are expressed in human osteoprogenitor cells and are involved in the osteogenic differentiation. The differential modulation of cadherin expression by osteotropic agents indicates that these agents may regulate osteoprogenitor cells through different cadherins and these cadherins may play different roles. | PengLIU Jian-haoLIN BinZHANG | 2005 | Acta Pharmacologica Sinica2005，26，6： | 3 |
| 2 | Microcalorimetric Study on Tyrosine Oxidation Catalyzed by Tyrosinase显示文摘Through the method of initial heat release rate, the kinetic property of tyrosine oxidationcatalyzed by tyrosinase from Pseudomonas maltophilia was investigated using a LKB-2107 batchmicrocalorimeter. Tyrosine was catalyzed and oxidized into L-dopa, then into melanin catalyzed bytyrosinase. We found that the tyrosinase reaction obeyed the Michaelis-Menten kinetics, and at298.15K and pH 7.0, the initial exothermic rate (Ω0) are in the range of0.1567~0.5704 mJ@ s-1, themaximum exothermic rate (Ωmax) are in 0.4152 ~ 0.8143mol @ L-1, and mean value of the Michaelisconstant (Km) is 2.199±0.105×104 mol @ L-1. | JunYAO YiLIU TianZhiWANG JianBenLIU PengLIU ZiNiuYU | 2003 | Chinese Chemical Letters2003，14，6： | 1 |
| 3 | A hybrid routing mechanism for ZigBee wireless sensor networks显示文摘 | Tan Pengliu Ju Mingshan | 2011 | Communications in Computer and Information Science2011，164，10： | 1 |
| 4 | Micro_ wave dielectric properties of low_temperature sintered Ca((Li1/3Nb2/3),Ti)O3_δ 显示文摘 | Hirotaka Ogawa Eung Soo Kim | 2004 | Eur Ceram Soc2004，24，： | 1 |
| 5 | TheBradenscalecannotbeusedaloneforassessingpressureulcerriskinsurgicalpatients:Ameta-analysis显示文摘 | WeiHe PengLiu Hong-LinChen | 2012 | OstomyWoundManagement2012，，2： | 1 |
| 6 | TemperatureandpHdual-responsive cross-linked polymeric nanocapsuleswithcontrollablestructuresviasurface-initiated atomtransferradical polymerization from templates 显示文摘 | BinMu PengLiu ZhaobinTang | 2011 | Nanomedicine:Nanotechnology Biologyand Medicine2011，7，： | 1 |
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