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您的检索式：作者名="Pavithra Sukumar"
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| 1 | AFM-compatible microfluidic platform for affinity- based capture and nanomechanical characterization of circulating tumor cells显示文摘Circulating tumor cells(CTCs)carried by the patient’s bloodstream are known to lead to the metastatic spread of cancer.It is becoming increasingly clear that an understanding of the nanomechanical characteristics of CTCs,such as elasticity and adhesiveness,represents advancements in tracking and monitoring cancer progression and metastasis.In the present work,we describe a combined microfluidic–atomic force microscopy(AFM)platform that uses antibody–antigen capture to routinely isolate and nanomechanically characterize CTCs present in blood samples from prostate cancer patients.We introduce the reversible assembly of a microfluidic device and apply refined and robust chemistry to covalently bond antibodies onto its glass substrate with high density and the desired orientation.As a result,we show that the device can efficiently capture CTCs from patients with localized and metastatic prostate cancer through anti-EpCAM,anti-PSA,and anti-PSMA antibodies,and it is suitable for AFM measurements of captured intact CTCs.When nanomechanically characterized,CTCs originating from metastatic cancer demonstrate decreased elasticity and increased deformability compared to those originating from localized cancer.While the average adhesion of CTCs to the AFM tip surface remained the same in both the groups,there were fewer multiple adhesion events in metastatic CTCs than there were in their counterparts.The developed platform is simple,robust,and reliable and can be useful in the diagnosis and prognosis of prostate cancer as well as other forms of cancer. | Muhammedin Deliorman Farhad K.Janahi Pavithra Sukumar Ayoub Glia Roaa Alnemari Samar Fadl Weiqiang Chen Mohammad A.Qasaimeh | 2020 | Microsystems & Nanoengineering2020，6，1： | 2 |
| 2 | Spike-and nucleocapsid-based gold colloid assay toward the development of an adhesive bandage for rapid SARS-CoV-2 immune response detection and screening显示文摘Immunoglobulin M(IgM)and immunoglobulin G(IgG)antibodies are important biomarkers used for the diagnosis and screening of severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)infections in both symptomatic and asymptomatic individuals.These antibodies are highly specific to the spike(S)and nucleocapsid(N)proteins of the SARS-CoV-2 virus.This paper outlines the development steps of a novel hybrid(vertical-lateral-vertical)flow assay in the form of a finger-stick point-of-care device,similar to an adhesive bandage,designed for the timely detection and screening of IgM and IgG immune responses to SARS-CoV-2 infections.The assay,comprising a vertically stacked plasma/serum separation membrane,conjugate pad,and detection(readout)zone,utilizes gold nanoparticles(AuNPs)conjugated with SARS-CoV-2 S and N proteins to effectively capture IgM and IgG antibodies from a pinprick(~15µL)of blood in just one step and provides results of no immune IgM−/IgG−,early immune IgM+/IgG−,active immune IgM+/IgG+or immune IgM−/IgG+in a short amount of time(minutes).The adhesive bandage-like construction is an example of the design of rapid,low-cost,disposable,and easy-to-use tests for large-scale detection and screening in households.Furthermore,the bandage can be easily adjusted and optimized to detect different viral infections as they arise by simply selecting appropriate antigens related to pandemics and outbreaks. | Imen Boumar Muhammedin Deliorman Pavithra Sukumar Mohammad A.Qasaimeh | 2023 | Microsystems & Nanoengineering2023，9，3： | 0 |
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