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| 1 | In vitro effects of polyphenols on colorectal cancer cells显示文摘AIM:To investigate the effects of quercetin and genistein on colon cancer cell proliferation and their estrogen receptorβ(ERβ)expression.METHODS:Colon cancer cells were stably transfected with a mammalian expression vector to overexpress ERβ(HCT8-β8-expressing cells)or a control vector(HCT8-pSV2neo-expressing cells).The proliferation of these cells was examined after treatment with quercetin or genistein(5-100μmol/L),or 10 nmol/L17β-estradiol(17β-E2).Cell viability was examined by acridine orange staining following treatments for 48 or144 h.Effects of quercetin and genistein on ERβtranscriptional transactivation were examined by luciferase activity in HCT8-β8-expressing cells transiently transfected with a pEREtkLUC reporter vector.In addition,the regulation of ERβtranscription by phytoestrogens and 17β-E2 was examined by quantitative polymerase chain reaction.RESULTS:Proliferation of HCT8-β8-expressing cells was not reduced low doses(5μmol/L)of quercetin and genistein,while it was reduced at 25-50μmol/L with an effect similar to 10 nmol/L 17β-E2.Treatment with doses of phytoestrogens≥75μmol/L completely blocked cell growth and reduced overall cell counts,however no effects at any dose were observed in HCT8-pSV2neoexpressing cells.These results were supported by viability staining that revealed acridine orange-stained lysosomes with high doses or extended treatment periods.Genistein and quercetin(50μmol/L)significantly increased ER-responsive luciferase activity similar to 10nmol/L 17β-E2(P<0.05).Furthermore,genistein and quercetin(50μmol/L),as well as 10 nmol/L 17β-E2significantly increased ERβmRNA levels in HCT8-β8-expressing cells(P<0.05).In addition,treatment of HCT8-pSV2neo-expressing cells with 50μmol/L quercetin or 10 nmol/L 17β-E2 significantly increased ERβmRNA levels compared to untreated controls(P<0.05),though the absolute levels were much lower than in HCT8-β8-expressing cells.CONCLUSION:The antitumorigenic effects of the phytoestrogenic compounds quercetin and genistein on colon cancers cells occur through ERβactivity and expression. | Barbara Pampaloni Gaia Palmini Carmelo Mavilia Roberto Zonefrati Annalisa Tanini Maria Luisa Brand | 2014 | World Journal of Gastrointestinal Oncology2014，6，8： | 2 |
| 2 | Terminology and classification of the cortical dysplasias显示文摘 | Palmini A Najm I Avanzini G | 2004 | Neurology2004，62，63： | 1 |
| 3 | Resection of the lesion in patients with hypothalamic hamartomas and cata- strophic epilepsy显示文摘 | Palmini A Chandler C Andermann F | 2002 | Neurology2002，58，9： | 1 |
| 4 | Terminology and classifica- tion of the cortical dysplasias显示文摘 | Palmini A Najm I Avanzini G | 2004 | Neurology2004，62，63： | 1 |
| 5 | The concept of the epileptogenic zone:a modern look at Penfield and Jasper' s views on the role of interictal spikes显示文摘 | Palmini A | | 0，，： | 1 |
| 6 | Terminology and classification of the cortical dysplasias显示文摘 | Palmini A Najm I Avanzini G et at | 2004 | Neurology2004，62，63： | 1 |
| 7 | Intrinsic epi- leptogenicity of human dysplastic cortex as suggested, by corticography and surgical results显示文摘 | Palmini A Gambardella A Andermann F | 1995 | Ann Neurol1995，37，4： | 1 |
| 8 | Revising the classification of focal cortical dysplasi- as显示文摘 | Palmini A | 2011 | Epilepsia2011，52，1： | 1 |
| 9 | Intrinsic epileptogenicity of human dy-splastic cortex as suggested by corticography and sugical results显示文摘 | Gambardella A Andermann F | 1995 | Ann Neurol1995，37，5： | 1 |
| 10 | A multicenter, open-label trial to evaluate the quality of life in adults with ADHD treated withlong-acting methylphenidate (OROS MPH): Concerta Quality of Life (CONQoL) study 显示文摘 | Mattos P Louza MR Palmini AL | 2013 | J Atten Disord2013，17，5： | 1 |
| 11 | Ultrasound image of a single symptomatic carotid stenosis disclosed as fibromusclar dysplasia显示文摘 | Simons PJ Paesschen WV Palmini A | 2004 | Neurology2004，62，： | 1 |
| 12 | Ictal perfusion patterns associated with single MRI- visible focal dysplastic lesions: implications for the noninvasive delineation of the epileptogenic zone 显示文摘 | Dupont P Van Paesschen W Palmini | 2006 | Epilepsia2006，47，： | 1 |
| 13 | Resection of the lesion in patients with hypothalarnic hamartornas and cata- strophic epilepsy 显示文摘 | Palmini A Chandler C Andermann F | 2002 | Neurology2002，58，9： | 1 |
| 14 | Terminology and :clas- sification of the cortical dysplasias显示文摘 | Palmini A Najm I Avanzini G | 2004 | Neurology2004，62，3： | 1 |
| 15 | Terminology and classification of the cortical dysplasias 显示文摘 | Palmini A Najm I Ltiders HO | 2004 | Neurology2004，62，63： | 1 |
| 16 | Ictal perfusion patterns associated with single MRI- visible focal dysplastic lesions: implications for the noninvasive delineation of the epileptogenic zone显示文摘 | Dupont P Van Paesschen W Palmini A | 2006 | Epilepsia2006，47，9： | 1 |
| 17 | Focal malformations of cortical devel- opment: a most relevant etiology of epilepsy in children 显示文摘 | Palmini A Holthausen H | 2013 | Handb Clin Neurol2013，111，： | 1 |
| 18 | Reflex seizures in patients with malformations of eortieal development and refractory epilepsy显示文摘 | Palmini A Halasz P Seheffer IE | 2005 | Epilepsia2005，46，8： | 1 |
| 19 | An 18-year follow up of seizure outcome after surgery for temporal lobe epi- lepsy and hippocampal scaerosis显示文摘 | Hemb M Palmini A Paglioli E | 2013 | J Neurol Neur Psy2013，84，7： | 1 |
| 20 | Terminology and classifica- tion of the cortical dysplasias显示文摘 | Palmini A Najm I Avanzini G | 2004 | Neurology2004，62，63： | 1 |
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