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| 1 | Indirect printing of hierarchical patient-specific scaffolds for meniscus tissue engineering显示文摘The complex meniscus tissue plays a critical role in the knee. The high susceptibility to injury has led to an intense pursuit for better tissue engineering regenerative strategies, where scaffolds play a major role. In this study, indirect printed hierarchical multilayered sca ffolds composed by a silk fibroin (SF) upper layer and an 80/20 (w/w) ratio of SF/ionic-doped β-tricalcium phosphate (TCP) bottom layer were developed. Furthermore, a comparative analysis between two types of sca ffolds pro- duced using di fferent SF concentrations, i.e., 8% (w/v) (Hi8) and 16% (w/v) (Hi16) was performed. In terms of architecture and morphology, the produced sca ffolds presented homogeneous porosity in both layers and no di fferences were observed when comparing both sca ffolds. A decrease in terms of mechanical performance of the sca ffolds was observed when SF concentration decreased from 16 to 8% (w/v). Hi16 revealed a static compressive modulus of 0.66 ± 0.05 MPa and dynamical mechanical properties ranging from 2.17 ± 0.25 to 3.19 ± 0.38 MPa. By its turn, Hi8 presented a compressive modulus of 0.27 ± 0.08 MPa and dynamical mechanical properties ranging from 1.03 ± 0.08 MPa to 1.56 ± 0.13 MPa. In vitro bioactivity studies showed formation of apatite crystals onto the surface of Hi8 and Hi16 bottom layers. Human meniscus cells (hMCs) and human primary osteoblasts were cultured separately onto the top layer (SF8 and SF16) and bottom layer (SF8/TCP and SF16/TCP) of the hierarchical sca ffolds Hi8 and Hi16, respectively. Both cell types showed good adhesion and proliferation as denoted by the live/dead staining, Alamar Blue assay and DNA quanti fication analysis. Subcutaneous implantation in mice revealed weak in flammation and sca ffold’s integrity. The hierarchical indirect printed SF sca ffolds can be promising candidate for meniscus TE sca ffolding applications due their suitable mechanical properties, good biological performance and possibility of being applied in a patient-speci fic approach. | Joao BCosta Joana Silva-Correia Sandra Pina Alain da Silva Morais Sílvia Vieira Hélder Pereira Joao Espregueira-Mendes Rui LReis Joaquim M.Oliveira | 2019 | Bio-Design and Manufacturing2019，2，4： | 2 |
| 2 | Trimeric autotransporters of Haemo philus parasuis , generation of an extensive passenger domain repertoire specific for pathogenic strains显示文摘 | Pina S Olvera A BarcelO A | 2009 | BacteriolJ2009，191，： | 1 |
| 3 | Genetic analysis of hepatitis A virus strains recovered from the environment and from patients with acute hepatitis 显示文摘 | Pina S Buti M Jardf R | 2001 | J Gen Virol2001，82，12： | 1 |
| 4 | Are ICTs improving transparency and accountability in the EU regional and local governments? An empirical study 显示文摘 | Pina V Torres L Royo S | 2007 | Public Administration2007，85，3： | 1 |
| 5 | Trimric autotransporters of Haemophilus parasuis:generation of an extensive passenger domain repertoire secific for pathogenic strains显示文摘 | Pina S Olvera A Barcelo A | 2009 | J Bacteriol2009，191，2： | 1 |
| 6 | Clinical role of exercisetraining in the management of patients with chronic heart failure显示文摘 | Keteyian S J Pina I L Hibner B S | 2010 | JCardiopuhn Rehabil Prev2010，30，2： | 1 |
| 7 | Extraintestinal pathogenic Escherichia coli 显示文摘 | James L S Pina M F Nereus W G | 2007 | Foodborne Pathog Dis2007，4，： | 1 |
| 8 | Thermal conffort criteria and building design: Field work in Portugal 显示文摘 | Manuel Correia Guedes Lul's Matias Carlos Pina Santos | 2009 | Renewable Energy2009，34，11： | 1 |
| 9 | Longitudinal study of hepatitis E virus infection in Spanish farrow-to-finish swine herds 显示文摘 | Casas M Cortes R Pina S | 2011 | Veterinary Microbiology2011，148，1： | 1 |
| 10 | Exercise and heart failure: A statement from the American Heart Association Committee on exercise, rehabilitation, and prevention显示文摘 | PINA I L APSTEIN C S BALADY G J | 2003 | Circu- lation2003，107，8： | 1 |
| 11 | Detection of hepatitis E virus in liv- er, mesenteric lymph node, serum,bile and faeces of naturally in- fected pigs affected by different pathological eonditions显示文摘 | Deus N Seminati C Pina S | 2007 | Vet- erinary Microbiology2007，119，24： | 1 |
| 12 | Hepatitis E virus epidemiology in industrialized countries显示文摘 | Clementre-Casares P Pina S Buti M | 2003 | Emerging Infectious Diseases2003，9，4： | 1 |
| 13 | Evaluation and insights into chitosan antimicrobial activity against anaerobic oral pathogens显示文摘 | Costa EM Silva S Pina C | | 0，，03： | 1 |
| 14 | Evaluation and insights into chitosan antimicrobial activity against anaerobic oral pathogens显示文摘 | Costa EM Silva S Pina C | | 0，，03： | 1 |
| 15 | Injectability of brushite-forrning Mg-substituted and Sr-substituted a-TCP bone cements显示文摘 | Pina S Torres P M C Ferreira J M F | 2010 | J Mater Sci: Mater Med2010，21，： | 1 |
| 16 | Clinical and laboratory characteristics of infectious mononucleosis by Epstein-Barr virus in Mexican children显示文摘 | González S Monroy Colín VA Pina Ruiz G | 2012 | BMC Res Notes2012，5，： | 1 |
| 17 | The kinetics of zincsilicate leaching in sodium hydroxide显示文摘 | Santos F M F Pina P S Porcaro R | 2010 | Hydrometallurgy2010，102，： | 1 |
| 18 | Recurrent temporal bone tenosynovial giant cell tumor with chondroid metaplasia : the use of imaging to assess recurrence 显示文摘 | Pina S Fernandez M Maya S | 2014 | Neuroradiol J2014，27，： | 1 |
| 19 | Occurrence of Helicobacter pylori in dental plaque and saliva of dyspeptic patients显示文摘 | Kignel S de Almeida Pina F Ander EA | 2005 | Oral Dis2005，11，： | 1 |
| 20 | Occurrence of Helicobacter pylori in dental plaque and saliva of dyspeptic patients 显示文摘 | Kignel S de Almeida Pina F Andre EA | 2005 | OralDis2005，11，： | 1 |
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