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| 1 | Interleukin-1α, 6 regulate the secretion of vascular endothelial growth factor A, C in pancreatic cancer显示文摘Vascular endothelial growth factor (VEGF, namely VEGF-A) is an angiogenic polypeptide and VEGF-C is a lymphangiogenic polypeptide that has been implicated in cancer growth, invasion and metastasis. Several cytokines and growth factors play an important part in cancer progression. These cytokines and growth factors are the principal mediators of cancer cells-stromal cell interaction , which is critical for invasion of cancer cells to the surrounding tissues and metastatic dissemination to distant organs. In this study, we studied VEGF-A, C expression in cultured human pancreatic cancer cell lines and whether the presence of VEGF-A, C in the cell lines is regulated by cytokines interleukin-lct (EL-1α), and interleukin-6 (IL-6). METHODS: We used Northern blot and Western blot methods to analyze expression of the gene and protein of VEGF-A, C in all 6 tested cell lines (ASPC-1, CAPAN-1, MIA-PaCa-2, PANC-1, COLO-357 and T3M4) respectively. To analyze what is the regulator for this VEGF-A, C expression in pancreatic cancer,we used the reverse transcription -polymerase chain reaction (RT-PCR) method to analyze VEGF-A, C expression in cultured human pancreatic cancer cell lines (CAPAN-1 and COLO-357) under the stimulation with IL-1α (10μg/L) or IL-6 (100 μg/L). RESULTS:Northern blot analysis revealed the presence of the 4.1-kb VEGF-A mRNA transcript and 2.4-kb VEGF-C mRNA transcript in all 6 tested cell lines. Immunoblotting with highly specific anti-VEGF-A, anti-VEGF-C antibody revealed the presence of a molecular weight of 43-kDa VEGF-A protein and 55-kDa VEGF-C protein in all the cell lines. RT-PCR analysis revealed the levels of the VEGF-A and VEGF-C gene were 1-2 fold and a 1-fold increase in the COLO-357 cell line by stimulation with IL-la, however, no effect was found in the CAPAN-1 cell line. The levels of the VEGF-A and VEGF-C gene were 2-5 fold and a 1-fold increase in the CAPAN-1 cell line by stimulation with IL-6, but, no effect was found in the COLO-357 cell line. CONCLUSION:These findings suggested that the expression of VEGF-A, C and their regulation by IL-1α, IL-6 in pancreatic cancer contributes to the lymphatic and distant metastasis and the disease progression. | Department of Hepatobiliary Surgery (Tang RF, Zhang FR, Peng L, Wang SX, Xiao Y and Zhang M) and Department of Dermatology (Wang SX), 4th Hospital, Hebei Medical University, Shijiazhuang 050011, China | 2005 | Hepatobiliary & Pancreatic Diseases International2005，4，3： | 6 |
| 2 | Hepatocytes isolated from neoplastic liver-immunomagnetic purging as a new source for transplantation显示文摘AIM: To investigate whether hepatocytes isolated from macroscopically normal liver during hepatic resection for neoplasia could provide a novel source of healthy hepatocytes, including the development of reliable protocols for malignant cells removal from the hepatocyte preparation. METHODS: Hepatocytes were procured from resected liver of 18 patients with liver tumors using optimised digestion and cell-enrichment protocols. Suspensions of various known quantities of the HT-29 tumor cell line and patient hepatocytes were treated or not with Ep-CAM-antibody-coated immunomagnetic beads in order to investigate the effi cacy of tumor-purging by immunomagnetic depletion, using a semi-quantitative RT-PCR method developed to detect tumor cells. Immunomagnetic bead-treated or bead-untreated tumor cell-hepatocyte suspensions were transplanted intra-peritoneally in Balb/C nude mice to assess the rates of tumor development. RESULTS: Mean viable hepatocyte yield was 9.3 x 106 cells per gram of digested liver with mean viability of 70.5%. Immunomagnetic depletion removed tumor cells to below the RT-PCR detection-threshold of 1 tumor cell in 106 hepatocytes, representing a maximum tumor purging efficacy of greater than 400 000-fold. Transplanted, immunomagnetic bead-purged tumor cell-hepatocyte suspensions did not form peritonealtumors in Balb/C nude mice. Co-transplantation of hepatocytes with tumor cells did not increase tumorigenesis of the tumor cells. CONCLUSION: Immunomagnetic depletion appears to be an effective method of purging contaminating tumor cells to below threshold for likely tumorigenesis. Along with improved techniques for isolation of large numbers of viable hepatocytes, normal liver resected for neoplasia has potential as another clinically useful source of hepatocytes | Aravin Gunasegaram Javed Akhter Peng Yao Loreena A Johnson Stephen M Riodan David L Morris | 2008 | World Journal of Gastroenterology2008，14，32： | 2 |
| 3 | Adaptive guaranteed cost control of systems with uncertain parameters显示文摘 | Chang M S L Peng T K C | 1972 | IEEE Transactions on Automatic Control1972，17，4： | 2 |
| 4 | Integrated Computer Program for Simulation of Meshing and Contact of Gear Drives 显示文摘 | LITVIN F L DONNO M D PENG A | 2000 | Computer Methods in Applied Mechanics and Engineering2000，181，13： | 1 |
| 5 | Reaction of N-methyl-2-pyrrolidinone with carbon disulfide显示文摘 | Zong Z M Peng Y L Qin Z H | 2000 | Energy Fuels2000，14，3： | 1 |
| 6 | PBDEs in sediments of the Beijiang River,China: levels,distribution,and influence of total organic carbon 显示文摘 | CHEN L G HUANG Y M PENG X C | 2009 | Chemosphere2009，76，2： | 1 |
| 7 | Shaping carbon nanotubes and the effects on their electrical and mechanical properties显示文摘 | Wang M S Peng L M Wang J Y | 2006 | Adv Funct Mater2006，16，11： | 1 |
| 8 | Nitrogen availability in disturbed,rehabilitated and mature forests of tropical China显示文摘 | MO J M BROWN S PENG S L | 2003 | Forest Ecology and Management2003，175，3： | 1 |
| 9 | Cyclosporin A traps ABCA1 at the plasma membrane and inhibits ABCA1-mediated lipid efflux to apolipoprotein A-I显示文摘 | Goff W L Peng D Q Settle M | 2004 | Arterioscler Thromb Vasc Biol2004，24，11： | 1 |
| 10 | The effect of exercise training on the metabolic interaction between digestion and locomotion in juvenile darkbarbel catfish (Peltebagrus vachelli) 显示文摘 | Li X M Cao Z D Peng J L | 2010 | Comparative Biochemistry and Physiology Part A2010，156，1： | 1 |
| 11 | Experimental study of electrical resistivity and flow stress of stainless steel 316L in electroplastic deformation显示文摘 | MAI J M PENG L F LIN Z Q | 2011 | Materials Science and EnginecringA2011，528，1011： | 1 |
| 12 | Self-assembled star-shaped chlorin-core poly (6-caprolaetone) -poly ( ethyleneglycol ) diblock copolymer micelles for dual ehemo-photodynamic therapies 显示文摘 | Peng C L Shieh M J Tsai M H | 2008 | Biomaterials2008，29，26： | 1 |
| 13 | Preparation and properties of high purity Mg-Y biomaterials显示文摘 | Peng Q M Huang Y D Zhou L Hort N Kainer K U | | 0，，： | 1 |
| 14 | The prevalence of parasites and pathogens in Asian honeybees Apis cerana in China显示文摘 | Li J L Qin H R Wu J Sadd B M Wang X Evans J D Peng W Chen Y | 2012 | PLoS One2012，7，47： | 1 |
| 15 | Multiscale entropy analysis of complex physiologic time series显示文摘 | Costa M Goldberger A L Peng C K | 2002 | Physical Review Letter2002，89，6： | 1 |
| 16 | Formation Mechanism of H2Ti3O7 Nanotubes显示文摘 | Zhang S Peng L M Chen Q | 2003 | Physical ReviewLetters2003，91，25： | 1 |
| 17 | Hopf bifurcation analysis for a ratio-dependent predator-prey system with two delays and stage structure for the predator 显示文摘 | DENG L W WANG X D PENG M | 2014 | Applied Mathematics and Computation2014，231，： | 1 |
| 18 | A comparative ownership advantage framework for cross - bor -der M&As : The rise of Chinese and Indian MNES 显示文摘 | SUN S L PENG M W REN B | 2012 | Journal of World Business2012，，47： | 1 |
| 19 | The TIL commissioning and performance显示文摘 | X Zhang W Zheng X Wei F Jing Z Sui K Zheng Q Xu X Yuan X Jiang L Yang P Ma M Li J Wang D Hu S He F Li Z Peng B Feng H Zhou L Guo X Li X Zhang J Su Q Zhu H Yu R Zhao C Ma H He D Fan W Zhang | 2008 | Journal of Physics: Conference Series2008，，3： | 1 |
| 20 | High temperature creepdeformation of A118B4O33 whisker-reinforced 8009 Al composite显示文摘 | L M Peng S J Zhu Z Y Ma | 1999 | Materials Science and Engineering1999，265，： | 1 |
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