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| 1 | CD4+ T cells and natural killer cells: Biomarkers for hepatic fibrosis in human immunodeficiency virus/hepatitis C virus-coinfected patients显示文摘AIM To characterize peripheral blood natural killer(NK) cells phenotypes by flow cytometry as potential biomarker of liver fibrosis in human immunodeficiency virus(HIV)/hepatitis C virus(HCV) coinfected patients.METHODS Peripheral mononuclear cells from 24 HIV/HCV(HBVnegative) coinfected and 5 HIV/HCV/HBV seronegative individuals were evaluated. HIV/HCV coinfected patients were divided in to groups: G1, patients with METAVIR F0-F2 and G2, patients with METAVIR F3-F4. NK surface cell staining was performed with: AntiCD3(APC/Cy7), anti-CD56(PE/Cy5), anti-CD57(APC), anti-CD25(PE), anti-CD69(FITC), anti-NKp30(PE), antiNKp46(PE/Cy7), anti-NKG2D(APC), anti-DNAM(FITC); anti-CD62L(PE/Cy7), anti-CCR7(PE), anti-TRAIL(PE), anti-Fas L(PE), anti CD94(FITC). Flow cytometry data acquisition was performed on BD FACSCanto, analyzed using Flow Jo software. Frequency of fluorescence was analyzed for all single markers. Clinical records were reviewed, and epidemiological and clinical data were obtained.RESULTS Samples from 11 patients were included in G1 and from 13 in G2. All patients were on ARV, with undetectable HIV viral load. Liver fibrosis was evaluated by transient elastography in 90% of the patients and with biopsy in 10% of the patients. Mean HCV viral load was(6.18 ± 0.7 log10). Even though, no major significant differences were observed between G1 and G2 regarding NK surface markers, it was found that patients with higher liver fibrosis presented statistically lower percentage of NK cells than individual with low to mild fibrosis and healthy controls(G2: 5.4% ± 2.3%, G1: 12.6% ± 8.2%, P = 0.002 and healthy controls 12.2% ± 2.7%, P = 0.008). It was also found that individuals with higher liver fibrosis presented lower CD4 LT count than those from G1(G2: 521 ± 312 cells/μL, G1: 770 ± 205 cells/μL; P = 0.035).CONCLUSION Higher levels of liver fibrosis were associated with lower percentage of NK cells and LTCD4+ count; and they may serve as noninvasive biomarkers of liver damage. | Natalia Laufer Diego Ojeda María Laura Polo Ana Martinez Héctor Pérez Gabriela Turk Pedro Cahn Norberto Walter Zwirner Jorge Quarleri | 2017 | World Journal of Hepatology2017，9，25： | 2 |
| 2 | Recombinant streptokinase vs hydrocortisone suppositories in acute hemorrhoids:A randomized controlled trial显示文摘AIM: To compare the efficacy and safety of recombinant streptokinase(rSK) vs hydrocortisone acetate-based suppositories in acute hemorrhoidal disease.METHODS: A multicenter(11 sites), randomized(1:1:1), open, controlled trial with parallel groups was performed. All participating patients gave their written,informed consent. After inclusion, patients with acute symptoms of hemorrhoids were centrally randomized to receive, as outpatients, by the rectal route, suppositories of rSK 200000 IU of one unit every 8 h(first 3 units)and afterwards every 12 h until 8 administrations were completed(schedule A), one unit every 8 h until 6 units were completed(schedule B), or 25 mg hydrocortisone acetate once every 8 h up to a maximum of 24 administrations. Evaluations were performed at 3, 5,and 10 d post-inclusion. The main end-point was the 5thday response(disappearance of pain and bleeding, and≥ 70% reduction of the lesion size). Time to response and need for thrombectomy were secondary efficacy variables. Adverse events were also evaluated.RESULTS: Groups were homogeneous with regards to demographic and baseline characteristics. Fifth day complete response rates were 156/170(91.8%; 95%CI:87.3-96.2), 155/170(91.2%; 95%CI: 86.6%-95.7%),and 46/170(27.1%; 95%CI: 20.1%-34.0%) with rSK(schedule A and B) and hydrocortisone acetate suppositories, respectively. These 64.6% and 63.9%differences(95%CI: 56.7%-72.2% and 55.7%-72.0%)were highly significant(P < 0.001). This advantage was detected since the early 3rd day evaluation(68.8% and64.1% vs 7.1% for the rSK and active control groups,respectively; P < 0.001) and was maintained even at the late 10 th day assessment(97.1% and 93.5% vs67.1% for rSK and hydrocortisone acetate, respectively;P < 0.001). Time to response was 3 d(95%CI: 2.9-3.1)for both rSK groups and 10 d(95%CI: 9.3-10.7) in the hydrocortisone acetate group. This difference was highly significant(P < 0.001). All subgroup stratified analyses(with or without thrombosis and hemorrhoid classification) showed a statistically significant advantage for the rSK groups. Thrombectomy was necessary in4/251 and 14/133 patients with baseline thrombosis in the rSK and hydrocortisone acetate groups, respectively(P < 0.001). There were no adverse events attributable to the experimental treatment.CONCLUSION: rSK suppositories showed a significant advantage over a widely-used over-the-counter hydrocortisone acetate preparation for the treatment of acute hemorrhoidal illness, as well as having an adequate safety profile. | Francisco Hernández-Bernal Georgina Castellanos-Sierra Carmen M Valenzuela-Silva Karem M Catasús-Alvarez Osmany Martínez-Serrano Odalys C Lazo-Diago Cimara H Bermúdez-Badell José R Causa-García Juan E Domínguez-Suárez Pedro A López-Saura 无 | 2015 | World Journal of Gastroenterology2015，21，23： | 2 |
| 3 | Sex-specific differences in white matter microvascular integrity after ischaemic stroke显示文摘background and purpose Functional outcomes after ischaemic stroke are worse in women,despite adjusting for differences in comorbidities and treatment approaches.White matter microvascular integrity represents one risk factor for poor long-term functional outcomes after ischaemic stroke.The aim of the study is to characterise sex-specific differences in microvascular integrity in individuals with acute ischaemic stroke.Methods A retrospective analysis of subjects with acute ischaemic stroke and brain MRI with diffusion-weighted(DWI)and dynamic-susceptibility contrast-enhanced(DSC)perfusion-weighted imaging obtained within 9 hours of last known well was performed.In the hemisphere contralateral to the acute infarct,normal-appearing white matter(NAWM)microvascular integrity was measured using the K_(2) coefficient and apparent diffusion coefficient(ADC)values.Regression analyses for predictors of K_(2) coefficient,DWI volume and good outcome(90-day modified Rankin scale(mRS)score<2)were performed.results 105 men and 79 women met inclusion criteria for analysis.Despite no difference in age,women had increased NAWM K_(2) coefficient(1027.4 vs 692.7×10^(-6)/s;p=0.006).In women,atrial fibrillation(β=583.6;p=0.04)and increasing NAWM ADC(β=4.4;p=0.02)were associated with increased NAWM K_(2) coefficient.In multivariable regression analysis,the K_(2) coefficient was an independent predictor of DWI volume in women(β=0.007;p=0.01)but not men.Conclusions In women with acute ischaemic stroke,increased NAWM K_(2) coefficient is associated with increased infarct volume and chronic white matter structural integrity.Prospective studies investigating sex-specific differences in white matter microvascular integrity are needed. | Mark R Etherton Ona Wu Pedro Cougo Svetlana Lorenzano Hua Li Lisa Cloonan Mark J R J Bouts Arne Lauer Ken Arai Eng H Lo Steve K Feske Karen L Furie Natalia S Rost | 2019 | Stroke & Vascular Neurology2019，4，4： | 2 |
| 4 | Safety from Crime and Physical Activity among Older Adults: A Pop- ulation--Based Study in Brazil显示文摘 | Maru W C Pedro C H Herton X C | 2012 | Journal of Environ- mental and Public Health2012，3，1： | 1 |
| 5 | Texture-mapping progressive meshes显示文摘 | Hopper H Pedro V Sander | 2001 | Computer Graphics (SIGGRAPH Proceedings)2001，35，： | 1 |
| 6 | Store brand and national brand promotion attitudes antecedents显示文摘 | ENRIQUE M SERGIO O PEDRO H | 2011 | Journal of Business Research2011，64，： | 1 |
| 7 | Evaluation of immediate endoscopic realignment as a treatment modality for traumatic urethral injuries显示文摘 | Pantelis H Kenji I Pedro GR | 2008 | J Trauma2008，64，6： | 1 |
| 8 | The spatial extent and dynamics of the Antarctic Cold Reversal显示文摘 | Pedro J B Bostock H C Bitz C M | 2016 | Nature Geoscience2016，9，： | 1 |
| 9 | Emulsion polymerization in a loop reactor: effect of the operation conditions显示文摘 | Araujo Pedro H H Abad Carlos de la Cal Jose C | 1999 | Polymer Reaction Engineering1999，7，3： | 1 |
| 10 | Recoverable rhodium nanoparticles : synthesis, characterization and eatalytic pegormance in hydrogenation reactions 显示文摘 | Mareos J Jacinto Pedro K Kiyohara Sueli H Masunaga | 2008 | Applied Catalysis A : General2008，338，12： | 1 |
| 11 | INSPM: An interactive evolutionary multi-objective algorithm with preference model显示文摘 | Pedro L R Takahashi R H | 2014 | Information Sciences2014，268，1： | 1 |
| 12 | An overview on subgroup discovery: foundations and applications 显示文摘 | FRANCISO H CRISTOBAL C J PEDRO G | 2011 | Soft Compu- ting2011，15，12： | 1 |
| 13 | Endoglin modulates cellular responses to TGF-β1显示文摘 | Pedro L Ainhoa L Zhang H W | 1996 | Cell Biol1996，133，： | 1 |
| 14 | Hydraulic conductivity of porous media at low water content显示文摘 | Toledo Pedro G Novy Robert A Davis H Ted | 1990 | Soil Sci Soc Am J1990，54，： | 1 |
| 15 | Disordered microbial communities in asthmatic airways显示文摘 | Hilty M Burke C Pedro H | 2010 | PLoS One2010，5，1： | 1 |
| 16 | Emulsion Polymerization in a Loop Reactor: Effect of the Operation Conditions显示文摘 | Araujo Pedro H H Abad C | 1999 | Polymer Reaction Engineering1999，7，3： | 1 |
| 17 | Influence of calcium nitrite inhibitor and crack width on corrosion of steel in high performance concrete subjected to a simulated marine environment显示文摘 | PEDRO M THEODERO W B DEREK H L | 2004 | Cement and Concrete Composites2004，26，3： | 1 |
| 18 | The effect of protein protein and protein mem- brane interactions on membrane fouling in ultrafiltration显示文摘 | Ingmar H Huisman Pedro Pr danos Antonio Hern ndez | 2000 | J Membr Sci2000，179，： | 1 |
| 19 | Ozone treatment for mercury determination in white wines显示文摘 | CAPELO J L PEDRO H A MOTA A M | 2003 | Talanta2003，61，4： | 1 |
| 20 | Duplicated fie genes in maize expression pattern and imprinting suggest distinct functions显示文摘 | DANILEVSKAYA O N PEDRO H SABINE H | 2003 | Plant Cell2003，15，： | 1 |
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