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| 1 | Visual detection of tropomyosin,a major shrimp allergenic protein using gold nanoparticles(AuNPs)-assisted colorimetric aptasensor显示文摘A gold nanoparticle-based label-free colorimetric assay was developed to detect the shrimp allergenic protein tropomyosin(TM),an important biomarker responsible for severe clinical reactivity to shellfish.In a gold nanoparticles(AuNPs)-tropomyosin-binding aptamer(TMBA)complex,the aptamer adsorbs onto the surface of AuNPs and dissociates in the presence of TM.In addition,AuNPs tend to aggregate in the presence of ionic salt,revealing a color change(i.e.,wine-red to purple/blue)with a shift in the maximum absorption peak from 520 nm.In the presence of specific binding TM,the aptamer folds into a tertiary structure where it more efficiently stabilizes AuNPs toward the salt-induced aggregation with a hypsochromic shift in the absorption spectra compared to the stabilized AuNPs by aptamer alone.Based on the aggregation and sensitive spectral transformation principle,the AuNPs-based colorimetric aptasensor was successfully applied to detect TM with a range of 10-200 nmol/L and a low detection limit of 40 nmol/L in water samples.The reliability,selectivity,and sensitivity of the aptasensor was then tested with food samples spiked with TM.The observed detection limit was as low as 70 nmol/L in shrimp,90 nmol/L in tofu,and 80 nmol/L in eggs,respectively.We anticipate the proposed AuNPs-based colorimetric aptasensor assay possesses a high potential for the easy and efficient visual colorimetric detection of TM. | Tushar Ramesh Pavase Hong Lin Maqsood Ahmed Soomro Hongwei Zheng Xiaxia Li Kexin Wang Zhenxing Li | 2021 | Marine Life Science & Technology2021，3，3： | 3 |
| 2 | 蛤蜊壳羟基磷灰石用于脱除蚝油中铅的初步研究显示文摘[目的]研究利用蛤蜊壳制备的羟基磷灰石用于吸附去除蚝油中重金属铅的可行性。[方法]利用含有铅离子的水溶液优化羟基磷灰石的吸附条件,进而探讨羟基磷灰石对蚝油中重金属铅的脱除效果,并分析脱除工艺对蚝油品质的影响。[结果]在羟基磷灰石用量为0.17 mg/m L,蚝油样品中铅离子含量为2.33 mg/m L以下时,羟基磷灰石对蚝油中重金属铅离子的吸附率达到98%以上,而且铅离子的浓度越高,吸附率越大。通过对吸附前后蚝油主要理化性质的对比发现,吸附作用对蚝油的主要品质指标没有显著影响,而且微量元素Ca、Zn的含量有所增加。[结论]用蛤蜊壳制备的羟基磷灰石能够有效吸附蚝油中的重金属铅,这不但为蚝油中重金属铅的控制提供了新的处理技术,而且还为蛤蜊壳的资源化及深度利用提供了新的思路。 | 金科 Tushar Ramesh Pavase 李振兴 徐凤娟 | 2015 | 中国渔业质量与标准2015，5，6： | 3 |
| 3 | Advanced Glycation Endproducts in 35 Types of Seafood Products Consumed in Eastern China显示文摘Advanced glycation endproducts(AGEs) have been recognized as hazards in processed foods that can induce chronic diseases such as cardiovascular disease, diabetes, and diabetic nephropathy. In this study, we investigated the AGEs contents of 35 types of industrial seafood products that are consumed frequently in eastern China. Total fluorescent AGEs level and Nε-carboxymethyl-lysine(CML) content were evaluated by fluorescence spectrophotometry and gas chromatography-mass spectrometry(GC-MS), respectively. The level of total fluorescent AGEs in seafood samples ranged from 39.37 to 1178.3 AU, and was higher in canned and packaged instant aquatic products that were processed at high temperatures. The CML content in seafood samples ranged from 44.8 to 439.1 mg per kg dried sample, and was higher in roasted seafood samples. The total fluorescent AGEs and CML content increased when seafood underwent high-temperature processing, but did not show an obvious correlation. The present study suggested that commonly consumed seafood contains different levels of AGEs, and the seafood processed at high temperatures always displays a high level of either AGEs or CML. | WANG Jing LI Zhenxing PAVASE Ramesh Tushar LIN Hong ZOU Long WEN Jie LV Liangtao | 2016 | Journal of Ocean University of China2016，15，4： | 1 |
| 4 | 肉类制品安全和质量监测的智能包装技术概述显示文摘食品包装行业自成立以来经历了长足的发展.在过去几十年里,包装行业的创新使得智能包装(SP)系统的发展,在竞争激烈的食品行业中占据了一席之地.SP系统除了它们的基本作用一保护产品不受有害的生物、化学和物理损害,并保持其清洁之外,在提高食品保质期和安全性方面具有巨大的潜力.指标和传感器,SP组件被用于实时监控肉类质量,和随后告知零售商和消费者产品的新鲜度、微生物、温度和保质期状况.在肉类包装中使用条码和无线电频率识别标签,以实时了解产品在供应链中的真实性和可追溯性.近年来,SP技术的创新使得肉和肉制品的新鲜度、微生物和保质期能够得到快速、灵敏和有效的检测、传感和保存记录.SP系统显示了对肉类行业广泛应用的承诺,以满足消费者对安全、优质肉制品以及减少浪费的观念需求.本文给出了更新正在进行的科学研究的概述,和最近的技术进步,发展智能的观点提供肉类包装系统能够监测的物理、微生物、化学变化的包装内容从生产商到销售点甚至更多,并修正这些潜在的不良反应. | Ishfaq Ahmed Hong Lin Long Zou Zhenxing Li Aaron L.Brody Ihsan Mabood Qazi Liangtao Lv Tushar Ramesh Pavase Mati Ullah Khan Sohaib Khan Lirui Sun | 2018 | 绿色包装2018，0，7： | 0 |
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