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| 1 | Angiogenesis and nerve regeneration induced by local administration of plasmid pBud-coVEGF165-coFGF2 into the intact rat sciatic nerve显示文摘Vascular endothelial growth factor(VEGF) and fibroblast growth factor 2(FGF2) are well-known growth factors involved in the regeneration of various tissues and organs, including peripheral nerve system. In the present study, we elucidated the local and systemic effects of plasmid construct рBud-coVEGF165-coFGF2 injected into the epineurium of intact rat sciatic nerve. Results of histological examination of sciatic nerve and multiplex immunoassays of serum showed the absence of immunogenicity and biosafety of plasmid рBud-coVEGF165-coFGF2. Moreover, local administration of plasmid DNA construct resulted in significantly decreased levels of pro-inflammatory cytokines in the peripheral blood, including tumor necrosis factor α(TNFα) and interleukin-12, and significantly increased levels of cytokines and chemokines including Regulated upon Activation, Normal T Cell Expressed and Presumably Secrete(RANTES), epidermal growth factor, interleukin-2, and monocyte chemoattractant protein 1. These changes in the peripheral blood on day 7 after injection of plasmid construct рBud-coVEGF165-coFGF2 show that the plasmid construct has systemic effects and may modulate immune response. At the same time, reverse transcriptionpolymerase chain reaction revealed transient expression of coFGF2, coVEGF165, ratFGF2 and ratVEGFA with direct transport of transcripts from distal part to proximal part of the sciatic nerve. Immunohistochemical staining revealed prolonged presence of VEGFA in sciatic nerve till 14 days post-injection. These findings suggest that local administration of plasmid construct рBud-coVEGF165-coFGF2 at a concentration of 30 ng/μL results in the formation of pro-angiogenic stimuli and, and the plasmid construct, used as a drug for gene therapy, might potentially facilitate regeneration of the sciatic nerve. The study was approved by the Animal Ethics Committee of Kazan Federal University, procedures were approved by the Local Ethics Committee(approval No. 5) on May 27, 2014. | Ruslan Masgutov Alina Zeinalova Alexey Bogov Galina Masgutova Ilnur Salafutdinov Ekaterina Garanina Valeriia Syromiatnikova Kamilla Idrisova Adelya Mullakhmetova Dina Andreeva Liliya Mukhametova Adilet Kadyrov Igor Pankov Albert Rizvanov | 2021 | Neural Regeneration Research2021，16，9： | 3 |
| 2 | Periodic and Decaying Solutions in Discrete Nonlinear Schr0dinger with Saturable Nonlinearity 显示文摘 | Pankov A Rothos V | 2008 | Proceedings of the Royal Society A: Mathematical Physical and Engineering Sciences2008，464，2100： | 1 |
| 3 | She and FAK differentially regulate cell motility and directionality modulated by PTEN显示文摘 | Gu J Tamura M Pankov R | 1999 | Cell Biol1999，146，2： | 1 |
| 4 | Taking cell-matrix adhesions to the third dimension显示文摘 | CUKIERMAN E PANKOV R STEVENS D R | 2001 | Science2001，294，5547： | 1 |
| 5 | Standing Waves of the Discrete Nonlinear Schrodinger Equations with Growing Potentials 显示文摘 | Zhang G Pankov A | 2008 | Com-mun Math Anal2008，5，： | 1 |
| 6 | The ion exchange phase in corrosion of nuclear waste glasses 显示文摘 | Ojovan M Pankov A Lee W | 2006 | Journal of Nuclear Materials2006，358，： | 1 |
| 7 | Shc and FAK differentially regulate cell motility and directionlity modlulstted by PTEN 显示文摘 | Gu J Tamura M Pankov R | 1999 | J Cell Biol1999，146，： | 1 |
| 8 | Effects of nitrogen nutrition on photosynthesis in Cd-treated sunflower plants显示文摘 | Pankovic D Plesnicar M Arsenijevic Maksimovic I | 2000 | Annals of Botany2000，86，4： | 1 |
| 9 | The water availability effect on the soil and vegetation cover of Southern Gobioases 显示文摘 | Pankov E I Kuzmina ZV Treshkin S E | 1994 | Water Resource1994，21，3： | 1 |
| 10 | Shc and FAK differentially regulate cell motility and directionality modulated by PTEN显示文摘 | Gu'J Tamura M Pankov R | 1999 | J Cell Bio11999，146，2： | 1 |
| 11 | GaN:from fundamentals to applications显示文摘 | PANKOVE J I | 1999 | Mater Sci Eng1999，6162，： | 1 |
| 12 | Kobuviruses-acomprehensive review显示文摘 | REUTER G BOROS A PANKOVICS P | 2011 | Rev Med Virol2011，21，1： | 1 |
| 13 | Involvement of proteolytic enzymes in the lipotropic effect of the pituitary polypeptide hormones显示文摘 | Pankov YA Keda YM Sazina ET | 1986 | Horm Metab Res1986，18，6： | 1 |
| 14 | Taking cell-matrix adhesions to the third dimension 显示文摘 | Cukierman E Pankov R Stevens DR | 2001 | Science2001，294，5547： | 1 |
| 15 | Evolution of porcine Kobuvirus infection, Hungary 显示文摘 | Reuter G Kecskemeti S Pankovics P | 2010 | Emerg Infect Dis2010，16，4： | 1 |
| 16 | Evolution of porcine kobuvi- rus infection 显示文摘 | Reuter G Kecskemetis Pankovics P | 2010 | Emerg Infect Dis2010，16，4： | 1 |
| 17 | Complete nucleotide and amino acid sequences and genetic organization of porcine kobuvirus,a member of a new species in the genus Kobuvirus,family Picornaviridae显示文摘 | Reuter G Boldizsár A Pankovics P | | 0，，01： | 1 |
| 18 | Nonlinear Schr0dinger equa- tions with steep potential well显示文摘 | Bartsch T Pankov A Wang Z Q | 2001 | Comm Contemp Math2001，4，： | 1 |
| 19 | Complete nucleotideand amino acid sequences and genetic organization of porcine kobu-virus ,a member of a new species in the genus kobuvirus,family po-comaviridae显示文摘 | REUTER G BOLSIZSAR A PANKOVICS P | 2009 | Arch Virol2009，154，1： | 1 |
| 20 | Optical Processes in Semiconducting Thin Films显示文摘 | Pankove J I | 1982 | Thin Solid Films1982，90，2： | 1 |
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