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| 1 | Effect of Moringa oleifera on semen quality profiles in endangered Teressa goat under tropical humid island ecosystem显示文摘Objective:To investigate the effect of Moringa oleifera leaf extract on semen quality parameters in Teressa goat buck.Methods:A total of 25 semen samples from six bucks were selected for the study.Spermatozoa of 150×10^(6) were incubated in 0,300,500 and 700μg of leaf extract as groupⅠ,Ⅱ,Ⅲ and Ⅳ,respectively.Liquid stored semen samples were analysed for motility,viability,total sperm abnormalities,plasma membrane,acrosomal and nuclear integrities,seminal plasma intracellular enzymes[aspartate aminotransferase(AST),alanine aminotransferase(ALT)and lactate dehydrogenase(LDH)]and total antioxidant capacity(TAC)and spermatozoa malondialdehyde(MDA)and cholesterol efflux in comparison with the control group(groupⅠ)for up to 72 h.Results:Moringa oleifera leaf extract(500μg/150×10^(6) spermatozoa)-treated semen had significantly higher motility,viability,plasma membrane,acrosomal and nuclear integrities,and TAC(P<0.05),and had significantly lower total sperm abnormalities,AST,ALT,LDH,MDA production and cholesterol efflux compared to those in other Moringa oleifera leaf extract treated(300 or 700μg/150×10^(6) spermatozoa)and control groups at different hours of liquid storage(P<0.05).Semen quality parameters and TAC showed an increasing trend and total sperm abnormalities,MDA production,leakage of intra-cellular enzymes and cholesterol efflux showed a decreasing trend from group Ⅰto group Ⅲand then an opposite trend from group Ⅲ to group Ⅳ at different hours of liquid storage.Conclusions:Moringa oleifera leaf extract 500μg/150×10^(6) spermatozoa can be used for liquid semen preservation in Teressa goat at Andaman and Nicobar Islands. | P.Perumal Jai Sunder A.K.De A.K.Nahak D.K.Chaurasia T.C.Balamurugan D.Bhattacharya | 2024 | Asian pacific Journal of Reproduction2024，13，2： | 0 |
| 2 | Seasonal stress on semen quality profiles, seminal biochemical and oxidative stress attributes in endangered Teressa goat of Andaman and Nicobar Islands显示文摘Objective:To measure seasonal effects on semen quality profiles,seminal biochemical and oxidative stress attributes in fresh and liquid stored semen in monsoon and dry seasons.Methods:A total of 10 Teressa bucks(3-4 years)were selected from breeding farm,ICAR-Central Island Agricultural Research Institute,Port Blair,Andaman and Nicobar Islands,India.Semen samples(n=25 per season)were collected through artificial vagina method and preserved at refrigerated temperature(5℃)for 48 h using Tris citrate glucose based extender.We detected semen quality parameters[volume,mass activity,pH,sperm concentration,total motility,viability,total sperm abnormality,and plasma membrane,acrosomal and nuclear integrities],biochemical profiles[aspartate amino transferase(AST),alanine amino transferase(ALT)and total cholesterol],and oxidative stress markers[total antioxidant capacity(TAC)and malondialdehyde(MDA)]during monsoon and dry seasons.Results:Semen quality parameters significantly differed between seasons(P<0.05)and among storage periods(P<0.05).Volume,pH,mass activity,motility,viability,acrosomal,plasma membrane and nuclear integrities,and TAC were significantly higher(P<0.05).Sperm concentration,sperm abnormalities,MDA,AST,ALT and total cholesterol were significantly lower in fresh semen of monsoon than dry season(P<0.05).Motility,viability,acrosomal,plasma membrane and nuclear integrities,and TAC were significantly decreased(P<0.05)while sperm abnormality,AST,ALT,total cholesterol and MDA were significantly increased as liquid semen storage period advanced(P<0.05).Conclusions:Monsoon season has higher beneficial effects on semen quality profiles and liquid stored semen remained usable for upto 48 h.Good quality ejaculates with higher TAC and lower MDA can be cryopreserved and will be used for artificial insemination. | P.Perumal Jai Sunder A.K.De D.Bhattacharya A.K.Nahak R.Vikram E.B.Chakurkar | 2023 | Asian pacific Journal of Reproduction2023，12，6： | 0 |
| 3 | Buffalo anti-PDC-109 antibodies improve the semen quality profiles and in-vitro zona binding index and minimize the cryoinjury of sperm in cryopreserved buffalo semen显示文摘Objective:To optimize the concentration of PDC-109 protein in semen for higher cryopreservability and fertility by sequestration or neutralization of PDC-109 by its antibodies(anti-PDC-109 antibodies)in bubaline species.Methods:PDC-109 protein was purified by applying two-step chromatography procedures.Purified protein was injected in rabbits to raise antibodies.These raised anti-PDC-109 antibodies were used in neutralization or sequestration of PDC-109 in in-vitro model.Ejaculates were collected from buffaloes and splited for four groups.Group 1 received egg yolk Tris glycerol extender,without anti-PDC-109 antibodies,while group 2 to 4 received anti-PDC-109 antibodies 266μg/mL,80μg/mL,and 26μg/mL in Tris-fructose-citrate buffer,respectively.Semen quality parameters viz.,forward progressive motility,viability,total morphological abnormality,acrosomal integrity,plasma membrane integrity,cryoinjury and in-vitro zona binding index were evaluated.Results:Semen quality parameters of neat semen were within the normal range of bubaline species.Sperm motility,livability,acrosomal integrity,plasma membrane integrity,and cholesterol content of sperm were decreased and total sperm abnormality was increased significantly in post-thaw semen compared to those in pre-freeze and fresh semen(P<0.05).Semen in group 2 had higher sperm motility,livability,acrosomal integrity,plasma membrane integrity,and cholesterol content of sperm and lower total sperm abnormality significantly compared to those in group 1,3 and 4 at pre-freeze and post-thaw stages(P<0.05).Conclusions:Sequestration or neutralization of PDC-109 by its antibodies significantly improves pre-freeze,and post-thaw semen quality parameters and in-vitro zona binding index with simultaneously reducing cryoinjury or cryodamage in the sperm of bubaline species. | S.S.Ramteke J.S.Rajoriya A.M.Shende S.K.Ghosh J.K.Prasad P.Perumal | 2023 | Asian pacific Journal of Reproduction2023，12，3： | 0 |
| 4 | Document Clustering Using Graph Based Fuzzy Association Rule Generation显示文摘With the wider growth of web-based documents,the necessity of automatic document clustering and text summarization is increased.Here,document summarization that is extracting the essential task with appropriate information,removal of unnecessary data and providing the data in a cohesive and coherent manner is determined to be a most confronting task.In this research,a novel intelligent model for document clustering is designed with graph model and Fuzzy based association rule generation(gFAR).Initially,the graph model is used to map the relationship among the data(multi-source)followed by the establishment of document clustering with the generation of association rule using the fuzzy concept.This method shows benefit in redundancy elimination by mapping the relevant document using graph model and reduces the time consumption and improves the accuracy using the association rule generation with fuzzy.This framework is provided in an interpretable way for document clustering.It iteratively reduces the error rate during relationship mapping among the data(clusters)with the assistance of weighted document content.Also,this model represents the significance of data features with class discrimination.It is also helpful in measuring the significance of the features during the data clustering process.The simulation is done with MATLAB 2016b environment and evaluated with the empirical standards like Relative Risk Patterns(RRP),ROUGE score,and Discrimination Information Measure(DMI)respectively.Here,DailyMail and DUC 2004 dataset is used to extract the empirical results.The proposed gFAR model gives better trade-off while compared with various prevailing approaches. | P.Perumal | 2022 | Computer Systems Science & Engineering2022，43，10： | 0 |
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