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| 1 | Quantitation of HBsAg predicts response to entecavir therapy in HBV genotype C patients显示文摘AIM:To analysis the factors that predict the response to entecavir therapy in chronic hepatitis patients with hepatitis B virus (HBV) genotype C. METHODS:Fifty patients [hepatitis B e antigen (HBeAg)-negative:HBeAg-positive = 26:24] with HBV genotype C, who received nave entecavir therapy for > 2 years, were analyzed. Patients who showed HBV DNA levels ≥ 3.0 log viral copies/mL after 2 years of entecavir therapy were designated as slow-responders, while those that showed < 3.0 log copies/mL were termed rapid- responders. Quantitative hepatitis B surface antigen (HBsAg) levels (qHBsAg) were determined by the Architect HBsAg QT immunoassay. Hepatitis B core-related antigen was detected by enzyme immunoassay. Pre-C and Core promoter mutations were determined using by polymerase chain reaction (PCR). Drug-resistance mutations were detected by the PCR-Invader method. RESULTS:At year 2, HBV DNA levels in all patients in the HBeAg-negative group were < 3.0 log copies/mL. In contrast, in the HBeAg-positive group, 41.7% were slow-responders, while 58.3% were rapid-responders. No entecavir-resistant mutants were detected in the slow-responders. When the pretreatment factors were compared between the slow-and rapid-responders; the median qHBsAg in the slow-responders was 4.57 log IU/mL, compared with 3.63 log IU/mL in the rapid-responders (P < 0.01). When the pretreatment factors predictive of HBV DNA-negative status at year 2 in all 50 patients were analyzed, HBeAg-negative status, low HBV DNA levels, and low qHBsAg levels were significant (P < 0.01). Multivariate analysis revealed that the low qHBsAg level was the most significant predictive factor (P = 0.03). CONCLUSION:Quantitation of HBsAg could be a useful indicator to predict response to entecavir therapy. | Etsuro Orito Kei Fujiwara Hiroshi Kanie Tesshin Ban Tomonori Yamada Katsumi Hayashi | 2012 | World Journal of Gastroenterology2012，18，39： | 8 |
| 2 | Classifying genotype F of hepatitis B virus into F1 and F2 subtypes显示文摘AIM: To explore the propriety of providing hepatitis B virus(HBV) genotypes F and H with two distinct genotypes.METHODS: Eleven HBV isolates of genotype F (HBV/F)were recovered from patients living in San Francisco,Japan, Panama, and Venezuela, and their full-length sequences were determined. Phylogenetic analysis was carried out among them along with HBV isolates previously reported.RESULTS: Seven of them clustered with reported HBV/F isolates in the phylogenetic tree constructed on the entire genomic sequence. The remaining four flocked on another branch along with three HBV isolates formerly reported as genotype H. These seven HBV isolates, including the four in this study and the three reported, had a sequence divergence of 7.3-9.5% from the other HBV/F isolates,and differed by > 13.7% from HBV isolates of the other six genotypes (A-E and G). Based on a marked genomic divergence, falling just short of >8% separating the seven genotypes, these seven HBV/F isolates were classified into F2 subtype and the former seven into F1 subtype provisionally. In a pairwise comparison of the S-gene sequences among the 7 HBV/F2 isolates and against 47HBV/F1 isolates as well as 136 representing the other six genotypes (A-E and G), two clusters separated by distinct genetic distances emerged.CONCLUSION: Based on these analyses, classifying HBV/F isolates into two subtypes (F1 and F2) would be more appropriate than providing them with two distinct genotypes (F and H). | Hideaki Kato Kei Fujiwara Robert G. Gish Hiroshi Sakugawa Hiroshi Yoshizawa Fuminaka Sugauchi Etsuro Orito Ryuzo Ueda Yasuhito Tanaka Takanobu Kato Yuzo Miyakawa Masashi Mizokami | 2005 | World Journal of Gastroenterology2005，11，40： | 6 |
| 3 | Electroacupuncture improves neuronal function by stimulation of ascending peripheral nerve conduction in rats with spinal cord injury显示文摘OBJECTIVE: To establish a method of transient sciatic nerve blockade and to examine the involvement of the ascending peripheral nerve pathway in the therapeutic effect of electroacupuncture at Zusanli(ST 36) in rats with spinal cord injury(SCI).METHODS: We examined the transient effect of daily lidocaine administration into the posteromedial part of the greater trochanter on sciatic nerve function using electrophysiological examination and histopathology of the sciatic nerve. Rats were divided into three groups: an SCI group(SCI without treatment), an SCI with electroacupuncture treatment(SCI-EA) group, and an SCI with nerve block and electroacupuncture(SCI-NB-EA) group(nerve block was achieved by lidocaine administration to transiently block the ascending peripheral nerve pathway). Behavioral tests and electrophysiological examinations were performed to evaluate recovery of neurological function.RESULTS: Sciatic nerve conduction was normal immediately before daily lidocaine administration.Histopathological analysis also indicated normal sciatic nerve, confirming that lidocaine nerve blockade was suitable and reversible for transiently eliminating nerve transmission. Neurological function in the SCI-EA group was superior to that in the SCI group, while no differences were found between the SCI and SCI-NB-EA groups.CONCLUSION: Electroacupuncture treatment can promote recovery of neurological function. Facilitation of nerve conduction may play an important role in this recovery. | Song Meng Chen Wu Kamiie Junichi Okuno Seiichi Orito Kensuke | 2019 | Journal of Traditional Chinese Medicine2019，39，4： | 5 |
| 4 | 乙型肝炎病毒基因型C亚型和核心启动子、前C/核心区基因变异的关系显示文摘目的研究慢性乙型肝炎病毒(HBV)感染者中HBV基因型C亚型(HBV/C)的核心启动子、前C/核心区基因变异情况,分析HBV/C亚型的病毒学特征。方法用酶联免疫法(ELISA)筛选出79例HBV/C,再用聚合酶链反应-限制性酶长度多态性分析方法(PCR-RFLP)进行HBV/C亚型分析;同时针对HBV核心启动子、preC/核心区基因进行半巢式PCR及PCR产物直接测序。结果①79例HBV/C中,33例(41·8%)为HBV/C1亚型,46例(58·2%)为HBV/C2亚型。②HBV/C1亚型仅见于来自中国南方的患者(P<0·0001)。③A1898位点变异仅见于HBV/C1亚型(P=0·056),V1753位点变异在HBV/C1亚型中多见(P<0·05);HBV/C2以T1858(90%)、A1896(40%)位点变异多见(P<0·008)。T1762/A1764位点变异在HBV/C两种亚型中均常见。④肝细胞癌(HCC)患者中,V1753和T1762/A1764变异最常见(P<0·05)。结论HBV/C1和HBV/C2在中国有明显的地区差异;V1753合并T1762/A1764双变异与发展为HCC相关,尤其在HBV/C1患者。 | 袁静 龚作炯 Masashi Mizokami Yasuhito Tanaka Fuat Kurbanov Etsum Orito 徐六妹 蒋小玲 赖伟珍 陆坚 周伯平 | 2006 | 中华实验和临床病毒学杂志2006，20，4： | 3 |
| 5 | Distribution of HBV genotypes among HBV carriers in Benin:phylogenetic analysis and virological characteristics of HBV genotype E显示文摘AIM: To determine the distribution of Hepatitis B virus (HBV) genotypes in Benin, and to clarify the virological characteristics of the dominant genotype.METHODS: Among 500 blood donors in Benin, 21 HBsAg-positive donors were enrolled in the study. HBV genotypes were determined by enzyme immunoassay and restriction fragment length polymorphism. Complete genome sequences were determined by PCR and direct sequencing.RESULTS: HBV genotype E (HBV/E) was detected in 20/21 (95.2%), and HBV/A in 1/21 (4.8%). From the age-specific prevalence of HBeAg to anti-HBe seroconversion (SC) in 19 HBV/E subjects, SC was estimated to occur frequently in late teens in HBV/E.The comparison of four complete HBV/E genomes from HBeAg-positive subjects in this study and five HBV/E sequences recruited from the database revealed that HBV/E was distributed throughout West Africa with very low genetic divers ity (nucleotide homology 96.7-99.2%).Based on the sequences in the basic core promoter (BCP)to precore region of the nine HBV/E isolates compared to those of the other genotypes, a nucleotide substitution in the BCP, G1757A, was observed in HBV/E.CONCLUSION: HBV/E is predominant in the Republic of Benin, and SC is estimated to occur in late teens in HBV/E. The specific nucleotide substitution G1757A in BCP, which might influence the virological characteristics,is observed in HBV/E. | Kei Fujiwara Yasuhito Tanaka Etsuro Orito Tomoyoshi Ohno Takanobu Kato Kanji Sugihara Izumi Hasegawa Mayumi Sakurai Kiyoaki Ito Atsushi Ozasa Yuko Sakamoto Isao Arita Ahmed El-Gohary Agossou Benoit Sophie I Ogoundele-Akplogan Namiko Yoshihara Ryuzo Ueda Masashi Mizokami | 2005 | World Journal of Gastroenterology2005，11，41： | 3 |
| 6 | New combination test for hepatitis C virus genotype and viral load determination using Amplicor GT HCV MONITOR test v2.0显示文摘AIM: To develop a new sensitive and inexpensive hepatitis C virus (HCV) combination test (HCV Guideline test) that enables the determination of HCV genotypes 1, 2 and 3,and simultaneous determination of HCV viral load using commercial Amplicor GT HCV MONITOR test v2.0 (microwell version).METHODS: The HCV Guideline test used the PCR product generated in commercial Amplicor GT HCV Monitor test v2.0 for viral load measurement using microwell plate version of Amplicor HCV Monitor and also captured on separate plates containing capture probes and competitive oligonucleotide probes specific for HCV genotypes 1, 2 and 3, The HCV genotype was subsequently determined using the biotin-labeled PCR product and five biotin-labeled HCV-specific probes.RESULTS: The sensitivity of the HCV Guideline test was 0.5 KIU/mL. Specificity of the HCV Guideline test was confirmed by direct sequencing of HCV core region and molecular evolutionary analyses based on a panel of 31 samples. The comparison of the HCV Guideline test and an in-house HCV core genotyping assay using 252 samples from chronic hepatitis C patients indicated concordant results for 97.2% of samples (59.5% genotype 1, 33.7% genotype 2, 6.0% genotype 3, and 0.8% mixed genotypes).Similarly, the HCV Guideline test showed concordance with a serological test, and the serological test failed to assign any serotype in 12.7% of the samples, indicating a better sensitivity of the HCV Guideline test.CONCLUSION: Clinically, both viral load and genotypes (1, 2 and 3) have been found to be major predictors of antiviral therapy outcome regarding chronic hepatitis C based on guidelines and they are, in normal circumstances,performed as separate stand-alone assays. The HCV Guideline test is a useful method for screening large cohorts in a routine clinical setting for determining the treatment regimen and for predicting the outcome of antiviral therapy of chronic hepatitis C. | Motokazu Mukaide Yasuhito Tanaka Hirokazu Kakuda Kei Fujiwara Fuat Kurbanov Eturo Orito Kentaro Yoshioka Kiyotaka Fujise Shoji Harada Takazumi Kozaki Kazuo Takemura Kazumasa Hikiji Masashi Mizokami | 2005 | World Journal of Gastroenterology2005，11，4： | 3 |
| 7 | Genotype and phylogenetic characterization of hepatitis B virus among multi-ethnic cohort in Hawaii显示文摘AIM: Hepatitis B virus (HBV) genomes in carriers from Hawaii have not been evaluated previously. The aim of thepresent study was to evaluate the distribution of HBV genotypes and their clinical relevance in Hawaii.METHODS: Genotyping of HBV among 61 multi-ethnicc arriers in Hawaii was performed by genetic methods.Three complete genomes and 61 core promoter/precore regions of HBV were sequenced directly.RESULTS: HBV genotype distribution among the 61 carderswas 23.0% for genotype A, 14.7% for genotype B and 62.3% for genotype C. Genotypes A, B and C were obtained from the carriers whose ethnicities were Filipino and Caucasian,Southeast Asian, and various Asian and Micronesian,respectively. All cases of genotype B were composed of recombinant strains with genotype C in the precore plus core region named genotype Ba. HBeAg was detected more frequently in genotype C than in genotype B (68.4% vs 33.3%, P<0.05) and basal core promoter (BCP) mutation (T1762/A1764) was more frequently found in genotype C than in genotype B. Twelve of the 38 genotype C strains possessed C at nucleotide (nt) position 1858 (C-1858).However there was no significant difference in clinical characteristics between C-1858 and T-1858 variants. Based on complete genome sequences, phylogenetic analysis revealed one patient of Micronesian ethnicity as having C1858 clustered with two isolates from Polynesia with T-1858.In addition, two strains from Asian ethnidties were clustered with known isolates in carriers from Southeast Asia.CONCLUSION: Genotypes A, B and C are predominant types among multi-ethnic HBV carriers in Hawaii, and distribution of HBV genotypes is dependent on the ethnic background of the carriers in Hawaii. | Mayumi Sakurai Fuminaka Sugauchi Naoky Tsai Seiji Suzuki Izumi Hasegawa Kei Fujiwara Etsuro Orito Ryuzo Ueda Masashi Mizokami | 2004 | World Journal of Gastroenterology2004，10，15： | 2 |
| 8 | A case-control study for clinical and molecular biological differences between hepatitis B viruses of genotypes B and C显示文摘 | Etsuro Orito Masashi Mizokami Hiroshi Sakugawa Kojiro Michitaka Kazuyoshi Ishikawa Takafumi Ichida Takeshi Okanoue Hiroshi Yotsuyanagi Shiro Iino | 2001 | Hepatology2001，，1： | 2 |
| 9 | Transforming growth factor-beta-1 genetic polymorphism in Japanese patients with chronic hepatitis C virus infection显示文摘 | SUZUKI S TANAKA Y ORITO E | 2003 | J Gastroenterol Hepatol2003，18，10： | 1 |
| 10 | Transforming growth factor-beta-1 genetic polymorphism in Japanese patients with chronic hepatitis C virus infection显示文摘 | Suzuki S Tanaka Y Orito E | 2003 | J Gastroenterol Hepatol2003，18，10： | 1 |
| 11 | Geographic distribution of hepatitis B virus (HBV) genotype in patients with chronic HBV infection in Japan 显示文摘 | ORITO E ICHIDA T SAKUGAWA H | 2001 | Hepatology2001，34，3： | 1 |
| 12 | Two subtypes(subgenotypes) of hepatiis B virus genotype C: A novel subtyping assay based on restriction fragment length polymorphism显示文摘 | Tanaka Y Orito E Yuen MF | 2005 | Hepatol Res2005，33，： | 1 |
| 13 | A calci?um-dependent protein kinase regulates Plasmodium ookinete access to the midgut epithelial cell显示文摘 | ISHINO T ORITO v CHINZEI v | 2006 | Mol Microbial2006，59，4： | 1 |
| 14 | 查看详情显示文摘 | Orito Y Imai S Niwa S | | 0，，： | 1 |
| 15 | Hepatitis B virus genotypes and clinical manifestation among hepatitis B carriers in Thailand显示文摘 | Sugauchi F Chutaputti A Orito E | 2002 | J Gastroenterol Hepatol2002，17，6： | 1 |
| 16 | Immature platelet fraction for prediction of platelet engraftment after allogeneic stem cell transplantation显示文摘 | Takami A Shibayama M Orito M | 2007 | Bone Marrow Transplant2007，39，8： | 1 |
| 17 | Host-independent evolution and a genetic classificatioa of the hepadnavirus family based on nucleotide sequences显示文摘 | Orito E Mizokami M Ina Y Moriyama E N Kameshima N Yamamoto M Gojobori T | 1989 | Proc Natl Acad Sci USA1989，86，： | 1 |
| 18 | TT virus infection among IVDUs in south western China 显示文摘 | Cao K Mizokami M Orito E | 1999 | Scand J Infect Dis1999，31，1： | 1 |
| 19 | Epidemiologic and virologic characteristics of hepatitis B virus genotype B having the recombination with genotype C显示文摘 | Sugauchi F Orito E Ichida T | 2003 | Gastroenterology2003，124，4： | 1 |
| 20 | Hepatitis B virus genotypes and clinical manifestation among hepatitis B carriers in Thailand显示文摘 | Sugauch F Chutaputti A Orito E | 2002 | Gastroenterol Hepatol2002，17，6： | 1 |
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