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| 1 | 2010年世界胃肠病学组织关于炎症性肠病诊断和治疗的实践指南显示文摘炎症性肠病(IBD)是一组特发性、慢性、炎症性肠道疾病状态,包括两种主要类型:克罗恩病(CD)和溃疡性结肠炎(UC),两者的临床和病理特征既有重叠又有区别。IBD过去常见于发达国家,近几十年,发展中国家的患病率逐步上升。本指南就IBD诊断和治疗方面提出的独特议题在当前的文献和指南中几乎从未被提及。由于各地区的IBD临床特点差异较大,因此根据患者的主诉诊断IBD或评估疾病活动度需随地区的变化而变化。同样,治疗亦应根据疾病的分类和当地医疗资源进行调整。世界胃肠病学组织根据世界各国医疗资源的差异,采用级联方法制订了IBD的诊疗指南。 | Charles N.Bernstein Michael Fried J.H.Krabshuis Henry Cohen R.Eliakim Suleiman Fedail Richard Gearry K.L.Goh Saheed Hamid Aamir Ghafor Khan A.W.LeMair Malfertheiner Qin Ouyang J.F.Rey Ajit Sood Flavio Steinwurz Ole O.Thomsen Alan Thomson Gillian Watermeyer 杨钊斌 杨川华 钱本余 | 2010 | 胃肠病学2010，15，9： | 101 |
| 2 | Detection of tumor stem cell markers in pancreatic carcinoma cell lines显示文摘BACKGROUND: Cancer of the pancreas is the fourth leading cause of cancer death in industrialized countries. In malignancy, actively proliferating cells may be effectively targeted and killed by anti-cancer therapies, but stem cells may survive and support re-growth of the tumor. Thus, new strategies for the treatment of cancer clearly will also have to target cancer stem cells. The goal of the present study was to determine whether pancreatic carcinoma cell growth may be driven by a subpopulation of cancer stem cells. Because previous data implicated ABCG2 and CD133 as stem cell markers in hematopoietic and neural stem/progenitor cells, we analyzed the expression of these two proteins in pancreatic carcinoma cell lines. METHODS: Five established pancreatic adenocarcinoma cell lines were analyzed. Total RNA was isolated and real- time RT-PCR was performed to determine the expression of ABCG2 and CD133. Surface expression of ABCG2 and CD133 was analyzed by flow cytometric analysis. RESULTS: All pancreatic carcinoma cell lines tested expressed significantly higher levels of ABCG2 than non-malignant fibroblasts or two other malignant non- pancreatic cell lines, i.e., SaOS2 osteosarcoma and SKOV3 ovarian cancer. Elevated CD133 expression was found in two out of five pancreatic carcinoma cell lines tested. Using flow cytometric analysis we confirmed surface expression of ABCG2 in all five lines. Yet, CD133 surface expression was detectable in the two cell lines, A818-6 and PancTu1, which exhibited higher mRNA levels.CONCLUSIONS: Two stem cell markers, ABCG2 and CD133 are expressed in pancreatic carcinoma cell lines. ABCG2 and/or CD133 positive cells may represent subpopulation of putative cancer stem cells also in this malignancy. Because cancer stem cells are thought to be responsible for tumor initiation and its recurrence after an initial response to chemotherapy, they may be a very promising target for new drug developments. | Monika Olempska Patricia Alice Eisenach Ole Ammerpohl Hendrik Ungefroren Fred Fandrich Holger Kalthoff | 2007 | Hepatobiliary & Pancreatic Diseases International2007，6，1： | 69 |
| 3 | Inflammatory pathways of importance for management of inflammatory bowel disease显示文摘Inflammatory bowel disease(IBD)is a group of chronic disorders of the gastrointestinal tract comprising Crohn’s disease(CD)and ulcerative colitis(UC).Their etiologies are unknown,but they are characterised by an imbalanced production of pro-inflammatory mediators,e.g.,tumor necrosis factor(TNF)-α,as well as increased recruitment of leukocytes to the site of inflammation.Advantages in understanding the role of the inflammatory pathways in IBD and an inadequate response to conventional therapy in a large portion of patients,has over the last two decades lead to new therapies which includes the TNF inhibitors(TNFi),designed to target and neutralise the effect of TNF-α.TNFi have shown to be efficient in treating moderate to severe CD and UC.However,convenient alternative therapeutics targeting other immune pathways are needed for patients with IBD refractory to conventional therapy including TNFi.Indeed,several therapeutics are currently under development,and have shown success in clinical trials.These include antibodies targeting and neutralising interleukin-12/23,small pharmacologic Janus kinase inhibitors designed to block intracellular signaling of several pro-inflammatory cytokines,antibodies targeting integrins,and small anti-adhesion molecules that block adhesion between leukocytes and the intestinal vascular endothelium,reducing their infiltration into the inflamed mucosa.In this review we have elucidated the major signaling pathways of clinical importance for IBD therapy and highlighted the new promising therapies available.As stated in this paper several new treatment options are under development for the treatment of CD and UC,however,no drug fits all patients.Hence,optimisations of treatment regimens are warranted for the benefit of the patients either through biomarker establishment or other rationales to maximise the effect of the broad range of mode-of-actions of the present and future drugs in IBD. | Jannie Pedersen Mehmet Coskun Christoffer Soendergaard Mohammad Salem Ole Haagen Nielsen | 2014 | World Journal of Gastroenterology2014，20，1： | 28 |
| 4 | 采用波纹管测试水泥基材料早期自收缩方法显示文摘用特制的波纹管模具,可以在塑性阶段以水平方向测长度的方式将体积应变转换为长度应变来测量,不仅能实现自浇注成型开始对水泥基材料自收缩的连续监测,而且可以有效避免重力、温度变化以及模具约束对于测试结果的影响。介绍了波纹管测长度的原理,研究了波纹管的材质、尺寸和内部空气对于测试结果的影响。结果表明:测试结果受到波纹管材质、尺寸和成型引入空气的影响;随着波纹管内试件的刚度不断增加,其影响程度也随之减小,到终凝10 h后这种影响可以忽略不计。 | 田倩 JENSEN Ole Mejlhede | 2009 | 硅酸盐学报2009，37，1： | 22 |
| 5 | miR-20b, miR-98, miR-125b-1*, and let-7e* as new potential diagnostic biomarkers in ulcerative colitis显示文摘AIM:To use microarray-based miRNA profiling of colonic mucosal biopsies from patients with ulcerative colitis (UC), Crohn's disease (CD), and controls in order to identify new potential miRNA biomarkers in inflammatory bowel disease. METHODS:Colonic mucosal pinch biopsies from the descending part were obtained endoscopically from patients with active UC or CD, quiescent UC or CD, as well as healthy controls. Total RNA was isolated and miRNA expression assessed using the miRNA microarray Geniom Biochip miRNA Homo sapiens (Febit GmbH, Heidelberg, Germany). Data analysis was carried out by principal component analysis and projection to latent structure-discriminant analysis using the SIMCA-P+12 software package (Umetrics, Umea, Sweden). The microarray data were subsequently validated by quantitative real-time polymerase chain reaction (qPCR) performed on colonic tissue samples from active UC patients (n = 20), patients with quiescent UC (n = 19), and healthy controls (n = 20). The qPCR results were analyzed with Mann-WhitneyU test.In silico prediction analysis were performed to identify potential miRNA target genes and the predicted miRNA targets were then compared with all UC associated susceptibility genes reported in the literature. RESULTS:The colonic mucosal miRNA transcriptome differs significantly between UC and controls, UC and CD, as well as between UC patients with mucosal inflammation and those without. However, no clear differences in the transcriptome of patients with CD and controls were found. The miRNAs with the strongest differential power were identified (miR-20b, miR-99a, miR-203, miR-26b, and miR-98) and found to be upregulated more than a 10-fold in active UC as compared to quiescent UC, CD, and controls. Two miRNAs, miR-125b-1* and let-7e*, were up-regulated more than 5-fold in quiescent UC compared to active UC, CD, and controls. Four of the seven miRNAs (miR-20b, miR-98, miR-125b-1*, and let-7e*) were validated by qPCR and found to be specifically upregulated in patients with UC. Usingin silico analysis we found several predicted pro-inflammatory target genes involved in various pathways, such as mitogen-activated protein kinase and cytokine signaling, which are both key signaling pathways in UC.CONCLUSION:The present study provides the first evidence that miR-20b, miR-98, miR-125b-1*, and let7e* are deregulated in patients with UC. The level of these miRNAs may serve as new potential biomarkers for this chronic disease. | Mehmet Coskun Jacob Tveiten Bjerrum Jakob Benedict Seidelin Jesper Thorvald Troelsen JΦrgen Olsen Ole Haagen Nielsen | 2013 | World Journal of Gastroenterology2013，19，27： | 19 |
| 6 | MicroRNAs in inflammatory bowel disease-pathogenesis,diagnostics and therapeutics显示文摘The pathogenesis of inflammatory bowel disease (IBD) is complex and largely unknown. Until recently, research has focused on the study of protein regulators in inflammation to reveal the cellular and molecular networks in the pathogenesis of IBD. However, in the last few years, new and promising insights have been generated from studies describing an association between an altered expression of a specific class of non-coding RNAs, called microRNAs (miRs or miRNAs) and IBD. The short (approximately 22 nucleotides), endogenous, single-stranded RNAs are evolutionary conserved inanimals and plants, and regulate specific target mRNAs at the post-transcriptional level. MiRNAs are involved in several biological processes, including development, cell differentiation, proliferation and apoptosis. Furthermore, it is estimated that miRNAs may be responsible for regulating the expression of nearly one-third of the genes in the human genome. Thus, miRNA deregulation often results in an impaired cellular function, and a disturbance of downstream gene regulation and signaling cascades, suggesting their implication in disease etiology. Despite the identification of more than 1900 mature human miRNAs, very little is known about their biological functions and functional targets. Recent studies have identified dysregulated miRNAs in tissue samples of IBD patients and have demonstrated similar differences in circulating miRNAs in the serum of IBD patients. Thus, there is great promise that miRNAs will aid in the early diagnosis of IBD, and in the development of personalized therapies. Here, we provide a short review of the current state-of-the-art of miRNAs in IBD pathogenesis, diagnostics and therapeutics. | Mehmet Coskun Jacob Tveiten Bjerrum Jakob Benedict Seidelin Ole Haagen Nielsen | 2012 | World Journal of Gastroenterology2012，18，34： | 18 |
| 7 | 物流运输调度问题的混沌烟花算法——基于多车型供应链显示文摘为了满足供应链物流的不同需求,考虑多种车型、车辆容量、车辆油耗、车辆最大配送距离等约束条件,以最小油耗、最短配送距离为目标,建立多车型供应链物流运输调度模型(Multi-Type Vehicle Routing Problem in Supply Chain,MTVRPSC),并提出一种混沌烟花算法求解该模型。该算法以烟花算法为核心,提出一种编解码策略实现连续空间到MTVRPSC离散空间的映射,重新定义算法的适应度函数、适应度值和适应度的比较方法,并采用混沌初始化策略和混沌搜索策略来增强算法收敛效果。实验结果表明,所提出的算法在求解MTVRPSC时具有较强的寻优能力和稳定性。 | 蔡延光 戚远航 蔡颢 陈厚仁 OLE Hejlesen | 2019 | 计算机工程与应用2019，55，3： | 17 |
| 8 | 泰森多边形的离散蝙蝠算法求解多车场车辆路径问题显示文摘本文提出一种泰森多边形的离散蝙蝠算法求解多车场车辆路径问题(multi-depot vehicle routing problem,MDVRP).所提出算法以离散蝙蝠算法为核心,融入了一种基于多车场多车辆问题的编解码策略.所提出算法还使用基于泰森多边形的初始化策略加快算法的前期收敛速度,采用基于向量比较机制的适应度函数来控制算法收敛的方向,引入基于近邻策略和优先配送策略的局部搜索算法来提高算法的寻优能力.实验结果表明:在合理的时间耗费内,所提出的算法能有效地求解MDVRP,尤其是带配送距离约束的MDVRP;相对于对比算法,所提出的算法表现出较强的寻优能力和稳定性. | 戚远航 蔡延光 蔡颢 黄何列 OLE Hejlesen | 2018 | 控制理论与应用2018，35，8： | 14 |
| 9 | 政府如何支持公私合营模式发展——20个欧洲国家PPP的政府支持情况评估比较显示文摘本文从制度的视角构建一个政府支持公私合营模式(Public Private Partnership,PPP)程度的指数——PPP政府支持指数(PPP Governmental Support Index,GSI)——以评估政府规定的制度体系对在交通基础设施和其他行业引入和推广PPP具有何种程度的促进或阻碍作用。首先,基于大量文献综述界定PPP政府支持指数的要素,包括关于PPP的政策和政治承诺、法律和监管框架,以及精心设计的PPP支持安排。其次,计算20个欧洲国家的PPP政府支持指数,将其分类并比较各国政府对基础设施领域PPP支持程度的异同。再次,探讨20个欧洲国家的制度指数得分与基础设施领域PPP行为的潜在联系。最后,讨论当前的PPP政府支持指数的潜力、实用性以及方法局限性,并详细阐述如何将该指数用于加强交通或其他领域PPP的比较研究中。 | Koen Verhoest Ole Helby Petersen Walter Scherrer Raden Murwantara Soecipto 张帆 | 2015 | 城市交通2015，13，4： | 13 |
| 10 | 2017欧洲心脏病学会/欧洲心胸外科协会心脏瓣膜病管理指南中经导管主动脉瓣置换术相关更新解读显示文摘自2012欧洲心脏协会/欧洲心胸外科协会关于心脏瓣膜病的管理指南发布以来,新的临床证据又大量积累。这些新证据使欧洲心脏协会/欧洲心胸外科协会需要更新瓣膜病的管理指南以达到心内科与心外科医师间的共识。该文将从经导管主动脉瓣置换术出发,解读2017年新指南所作出的更新。 | Tasalak Thonghong Manik Chopra Ole De Backer Lars Sndergaard 王玺 | 2018 | 华西医学2018，33，2： | 12 |
| 11 | 长效胰岛素类似物的应用现状显示文摘 | Ole Schmitz | 2006 | 国际内分泌代谢杂志2006，26，3： | 12 |
| 12 | Manufacturing tolerant topology optimization显示文摘In this paper we present an extension of thetopology optimization method to include uncertainties dur-ing the fabrication of macro,micro and nano structures.Morespecifically,we consider devices that are manufactured usingprocesses which may result in(uniformly)too thin(eroded)or too thick(dilated)structures compared to the intendedtopology.Examples are MEMS devices manufactured usingetching processes,nano-devices manufactured using e-beamlithography or laser micro-machining and macro structuresmanufactured using milling processes.In the suggestedrobust topology optimization approach,under- andover-etching is modelled by image processing-based 'erode'and 'dilate' operators and the optimization problem is for-mulated as a worst case design problem.Applications ofthe method to the design of macro structures for minimumcompliance and micro compliant mechanisms show that themethod provides manufacturing tolerant designs with littledecrease in performance.As a positive side effect the robustdesign formulation also eliminates the longstanding prob-lem of one-node connected hinges in compliant mechanismdesign using topology optimization. | Ole Sigmund | 2009 | Acta Mechanica Sinica2009，25，2： | 12 |
| 13 | 医学伦理学——欧洲与中国的桥显示文摘跨文化医学伦理学研究 ,要求我们以理智的方式去应对全球化。既要维护多样性 ,又要明确共同利益、亦要追求共同目标。任何文化都不能强行提出一些标准去规范国际间的生物医学。自主权应坚持义务论的解释 ,否则一个强势风气将会支配利益的选择解释 ,将文化多样性在伦理学中铸成一个统一的模式 ,形成自主权的“通货膨胀倾向”。 | Ole Doering 赵明杰 | 2002 | 医学与哲学2002，23，6： | 11 |
| 14 | Impact of fine particles in ambient air on lung cancer显示文摘Recently,the International Agency for Research on Cancer(IARC)has classified outdoor air pollution and the particulate matter component of outdoor air pollution as class I carcinogen.Air pollution is consistently associated with lung cancer in epidemiologic and experimental studies.The IARC assessment is specifically designed as hazard identification,and it does not quantify the magnitude of the cancer risk.This article addresses the magnitude of the lung cancer risk in the population due to ambient air pollution exposure. | Gerard Hoek Ole Raaschou-Nielsen | 2014 | Chinese Journal of Cancer2014，33，4： | 10 |
| 15 | Exploiting Additive Manufacturing Infill in Topology Optimization for Improved Buckling Load显示文摘增材制造可实现优质多功能构件所具有的高度复杂几何构型的制备。可以直接制备内含多孔填充的结构部件是其独有特征的一个例证。现有的设计方法还难以充分利用这一设计自由度,直接获得类似结构的设计。本文将展示涂层方法 (coating approach)的拓扑优化方法来作为多孔填充构件的设计方法,所设计的构件具有显著改进的临界屈曲载荷,从而使得整体结构部件的稳定性增强。传统的柔顺性拓扑优化方法极少在数学模型中考虑构件的屈曲约束,稳定性要求通常要经过后续的校核与改进过程满足。这一后续过程往往只能获得次优设计。本文所展示的方法弥补了传统柔顺性拓扑优化模型中难以考虑构件屈曲约束的缺陷。利用涂层拓扑优化方法与传统柔顺性拓扑优化同时对经典的MBB梁进行设计,并采用熔丝增材制造技术对设计结果进行了制备。实验结果验证了涂层方法的数学模型的正确性。由于填充材料的性质,在相同条件下,涂层优化得到的多孔填充结构的屈曲载荷比传统优化得到的实体结构高四倍以上。 | Anders Clausen Niels Aage Ole Sigmund | 2016 | Engineering2016，2，2： | 9 |
| 16 | Modulation of Gut Microbiota in Pathological States显示文摘人类微生态是寄居在人体中的微生物聚集体,且主要存在于胃肠道(GIT)中。肠道微生态随着人体发育而演化,并在人类健康和疾病中起着重要作用。近年来,由于微生态会影响宿主代谢、生理学和免疫系统发育,而且微生态紊乱可能导致许多疾病,其越来越受到人们的关注。肠道微生态可能与恶性肿瘤有一定联系,如胃癌和结直肠癌;也可能与其他一些疾病有关,如非酒精性脂肪肝(NAFLD)、被称为工业化世界'生活方式疾病'的肥胖和糖尿病、冠心病以及中枢神经系统紊乱。虽然分子技术革命为我们更准确地研究肠道微生态提供了必要的工具,但是我们需要更精确地阐明其与某些人类疾病病理变化的关系,明确微生态在不同疾病中的作用是新的治疗策略发展的基础。本文概述了肠道微生态对人类健康的重要影响以及调整肠道菌群结构的潜在用途,如菌群移植用于治疗耐药艰难梭菌(C.difficile)的感染。通过微生态干预调整肠道区域以改善人类健康的概念虽刚刚兴起,但其治疗意义显著。因此,抑制有害菌、促进有益菌可能会保护人类健康,并且这些努力将为探索发展更加合理的治疗方案打下基础。 | Yulan Wang BaohongWang Junfang wu xiangyang Jiang Huiru Tang Ole H. Nielsen | 2017 | Engineering2017，3，1： | 9 |
| 17 | Real-time high-resolution mid-infrared optical coherence tomography显示文摘The potential for improving the penetration depth of optical coherence tomography systems by using light sources with longer wavelengths has been known since the inception of the technique in the early 1990s.Nevertheless,the development of mid-infrared optical coherence tomography has long been challenged by the maturity and fidelity of optical components in this spectral region,resulting in slow acquisition,low sensitivity,and poor axial resolution.In this work,a mid-infrared spectral-domain optical coherence tomography system operating at a central wavelength of 4μm and an axial resolution of 8.6μm is demonstrated.The system produces two-dimensional cross-sectional images in real time enabled by a high-brightness 0.9-to 4.7-μm mid-infrared supercontinuum source with a pulse repetition rate of 1 MHz for illumination and broadband upconversion of more than 1-μm bandwidth from 3.58–4.63μm to 820–865 nm,where a standard 800-nm spectrometer can be used for fast detection.The images produced by the mid-infrared system are compared with those delivered by a state-of-the-art ultra-high-resolution near-infrared optical coherence tomography system operating at 1.3μm,and the potential applications and samples suited for this technology are discussed.In doing so,the first practical mid-infrared optical coherence tomography system is demonstrated,with immediate applications in real-time non-destructive testing for the inspection of defects and thickness measurements in samples that exhibit strong scattering at shorter wavelengths. | Niels M.Israelsen Christian R.Petersen Ajanta Barh Deepak Jain Mikkel Jensen Gunther Hannesschlager Peter Tidemand-Lichtenberg Christian Pedersen Adrian Podoleanu Ole Bang | 2019 | Light(Science & Applications)2019，8，1： | 7 |
| 18 | 德国卫生保健体系改革及相关伦理问题简析显示文摘从德国现有卫生保健及医疗保险体系在保障其社会稳定的重要作用角度出发,介绍了德国的原有整体医疗卫生保障体系的背景,指出政府的指导、政策稳定、完善的津贴体系保障了整体国民健康和卫生保健的公平分配,高素质,医术高超的医生队伍起到了核心作用。同时指出由于意识形态和经济原因,德国卫生保健体系改革所引发的问题。 | Ole Doering 王卉 张斌 | 2007 | 医学与哲学(A)2007，28，10： | 7 |
| 19 | 冲绳海槽北部的全新世火山碎屑沉积显示文摘分析了冲绳海槽北部的CSH1岩芯沉积物中矿物组成、化学元素质量分数和粒度的变化规律,研究结果表明,在全新世期间,日本九州岛发生了2次大规模火山喷发活动,造成大量的火山物质沉积在冲绳海槽北部。从岩芯最上部的沉积物中可识别出2层火山碎屑沉积,它们都具有沉积物粒径突然变粗、SiO2和Na2O等化合物的质量分数突然增加以及粘土矿物、挥发份、Fe2O3、MgO、CaO和Sr的质量分数迅速下降等特征。沉积物粒度数据的端员分析结果表明,冲绳海槽北部全新世沉积物中的硅酸盐碎屑是由陆源碎屑物质和火山灰按不同的比例混合而成的。有火山物质混入的沉积物的粒度分布多呈现双峰模式,粒度众数值分别为74.3和7.81μm。浮游有孔虫壳体的AMS^14C测年结果表明,2层火山碎屑所对应的火山喷发年代分别为7210和10870cal.aBP。其中前者与K—Ah火山的喷发时间一致,后者可能与Kuju火山在12~10cal.kaBP期间的喷发有关。 | 刘焱光 曹东林 张德玉 孙吉林 NIELSEN Ole Bjφerslev 石学法 | 2007 | 海洋科学进展2007，25，1： | 6 |
| 20 | Impacts of livestock grazing on a savanna grassland in Kenya显示文摘The dynamics of most rangelands in Kenya remain to be poorly understood.This paper provides baseline information on the response of a semiarid rangeland under different livestock grazing regimes on land inhabited by the Massai people in the east side of Amboseli National Park in Kenya.The data were collected from grasslands designated into four types:(1) grassland from previous Massai settlements that had been abandoned for over twenty years;(2) grassland excluded from livestock grazing for eight years;(3) a dry season grazing area;and (4) a continuous grazing area where grazing occurred throughout all seasons.Collected data included grass species composition,grass height,inter-tuft distance,standing grass biomass and soil characteristics.The results indicated that continuous grazing area in semiarid rangelands exhibited loss of vegetation with negative,long-term effects on grass functional qualities and forage production,whereas grassland that used traditional Maasai grazing methods showed efficiency and desirable effects on the rangelands.The results also showed that abandoned homestead sites,though degraded,were important nutrient reservoirs. | John KIOKO John Warui KIRINGE Simon Ole SENO | 2012 | Journal of Arid Land2012，4，1： | 5 |
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