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| 1 | Optimizing bio-physical conditions and pre-treatment options for breaking lignin barrier of maize stover feed using white rot fungi显示文摘The greatest limitation to utilization of maize stover by ruminants as a feed is the high concentration of lignin, which limits fibre digestibility. However, ruminants can effectively utilize maize stover if its nutritive value is improved using white rot fungal species. This study was designed to determine optimal biophysical conditions for mycelial growth and select the most ideal fungal species and pre-treatment options for improving nutritive value of maize stover. Four popular edible Pleurotus fungal species(viz.Pleurotus florida,Pleurotus ostreatus, Pleurotus sajor caju and Pleurotus pulmonarius) were subjected to varying temperatures, pH levels, hydrogen peroxide(H_2 O_2) concentration and illumination to establish the extent of mycelial growth rate.Inclusion of H_2 O_2 was used to determine optimal levels for preservation and prevention of contamination from other indigenous microbiota. Effects of pre-treatment options on chemical composition and nutritive value of maize stover were also examined. Mycelial growth rate of Pleurotus species on potato dextrose agar(PDA) varied(P < 0.05) with temperature, pH level and H_2 O_2 concentration following a quadratic trend. Optimal temperature, pH and H_2 O_2 concentration for mycelial growth on PDA were 25 ℃,5 and 0.01 mL/L, respectively. Under the different bio-physical conditions,P. sajor caju had the highest mycelia density and growth rate. Chemical composition of solid-state fermented maize stover differed(P < 0.05) among the Pleurotus species. Maize stover fermented with P. sajor caju had the highest crude protein(CP) of 86.6 g/kg DM, in-vitro dry matter digestibility(IVDMD) of 731 g/kg DM, in-vitro organic matter digestibility(IVOMD) of 670.4 g/kg DM and metabolizable energy(ME) of10.0 MJ/kg DM but with the lowest lignin(sa) of 50 g/kg DM. At 25℃, P. sajor caju had the highest mycelial growth rate on PDA and highest lignin(sa) breakdown in the maize stover substrate. It was, therefore,selected as the most ideal fungal species for improving nutritive value of maize stover. Pre-treatment of maize stover with Lactobacillus plantarum and molasses under anaerobic condition for 7 days before inoculation with P. sajor caju resulted into a substrate with the highest(P< 0.05) CP(96.6 g/kg DM), IVDMD(752.3 g/kg DM), IVOMD(687.2 g/kg DM) and ME(10.2 MJ/kg DM). However, neutral detergent fiber exclusive of residual ash(NDFom) and lignin(sa) fractions decreased(P < 0.05) as a result of subjecting maize stover to pre-treatment with L. plantarum and molasses prior to fermentation with P. sajor caju.Therefore, pre-treatment of maize stover with L. plantarum and molasses for 7 days prior to fermentation with P. sajorcaju for 14 days in darkness at 25℃ offered the greatest potential for breaking the lignin barrier. | Andrew M. Atuhaire Fred Kabi Samuel Okello Swidiq Mugerwa Cyprian Ebong | 2016 | Animal Nutrition2016，，4： | 2 |
| 2 | The impact of group counseling on depression, post-traumatic stress and function outcomes: A prospective comparison study in the Peter C. Alderman trauma clinics in northern Uganda显示文摘 | Etheldreda Nakimuli-Mpungu James Okello Eugene Kinyanda Stephen Alderman Juliet Nakku Jeffrey S. Alderman Alison Pavia Alex Adaku Kathleen Allden Seggane Musisi | 2013 | Journal of Affective Disorders2013，，： | 2 |
| 3 | The relative importance of large mammal species for tourism in Amboseli national park, Kenya 显示文摘 | Okello M M Manka S G Amour D E D | 2008 | Tourism Management2008，29，4： | 1 |
| 4 | Maximum likelihood registration for multiple dissimilar radars显示文摘 | OKELLO N N RISTIC B | 2003 | IEEE Transactions on Aerospace and Electronic Systems2003，39，3： | 1 |
| 5 | Effects of Tour Guides Training on Their Performance in Kenya显示文摘 | Francis Kabii Moses Makonjio Okello Nehemiah Kipruto | 2017 | Journal of Tourism and Hospitality Management2017，5，6： | 1 |
| 6 | Conversion of amyloid positive and negative MCI to AD over 3 years: an 11C-PIB PET study显示文摘 | Okello A Koivunen J Edison P | 2009 | Neurology2009，73，10： | 1 |
| 7 | Land use changes and human wildlife conflicts in the Amboseli Area,Kenya 显示文摘 | Okello M M | 2005 | Human Dimensions of Wildlife2005，10，： | 1 |
| 8 | Fructose-derived ionic liquids: Recyclable homogeneous supports 显示文摘 | Handy S T Okello M | 2003 | Tetrahedron Lett2003，44，46： | 1 |
| 9 | Treatment with spimnolactone for 24 weeks decreases the level of matrix metalloproteinases and improves cardiac function in patients with chronic heart failure of ischemie etiology 显示文摘 | Li MJ Huarrg CX Okello E | 2009 | Can J Cardiol2009，25，9： | 1 |
| 10 | Maximum likelihood registration for multiple dissimilar sensors 显示文摘 | Okello N Ristie B | 2003 | IEEE Trans on AES2003，39，3： | 1 |
| 11 | Cost and cost - effective- ness of community - based care for tuberculosis patients in rural Uganda显示文摘 | Okello D Floyd K Adatu F | 2003 | The International Journal of Tuberculosis and Lung Disease2003，7，9： | 1 |
| 12 | 卡维地洛对连接蛋白43的分布及数量的影响显示文摘目的观察卡维地洛对心肌梗死边缘区Cx43分布的影响。方法36只家兔随机分为梗死组,卡维地洛组,假手术组,每组12只。梗死组和卡维地洛组开胸结扎冠状动脉左前降支,假手术组不结扎。所有家兔普通饲料喂养,卡维地洛组另给予卡维地洛5mg·kg^(-1)·d^(-1)。12周后观察:(1)共聚焦激光显微镜观察梗死边缘区Cx43的分布,(2)Werstern Blot检查梗死边缘区Cx43的蛋白表达。结果(1)共聚焦激光显微镜下观察心肌梗死组Cx43荧光斑的形态相对粗乱,呈现不均一分布;卡维地洛组Cx43的荧光分布改变较少,不均一分布的程度较心肌梗死组减轻;心梗组的Cx43相对密度显著低于卡维地洛组;[(0.16±0.06)%vs(0.32±0.11)%,P<0.05];(2)Werstern Blot检查梗死组Cx43及卡维地洛组较假手术组减少,但卡维地洛组的Cx43表达较心肌梗死组增多[(0.89±0.28)vs(0.67±0.32),P<0.05]。结论卡维地洛可以引起Cx43的再分布,增加心肌梗死后Cx43蛋白的表达,这可能是其较减少心肌梗死后室性心律失常的原因之一。 | 陈葵 唐艳红 黄从新 王唏 Emmy Okello 包明威 邓汉华 胡曙萍 葛建 | 2007 | 中华急诊医学杂志2007，16，10： | 1 |
| 13 | One Health and the neglected zoonoses: turning rhetoric into reality显示文摘 | Okello A L Gibbs E P Vandersmissen A | 2011 | Vet Rec2011，169，11： | 1 |
| 14 | Conversion of amyloid posi- tive and negative MCI to AD over 3 years: an llC-PIB PET study 显示文摘 | Okello A Koivunen J Edison P | 2009 | Neurology2009，73，10： | 1 |
| 15 | Treatment with spironolactone for 24 weeks decreases the level of matrix metalloproteinases and improves cardiac function in patients with chronic heart failure of ischemic etiology 显示文摘 | Li M J Huang CX Okello E | 2009 | Can J Cardiol2009，25，9： | 1 |
| 16 | Maximum likelihood registration for multiple dissimilar sensors显示文摘 | Okello N Ristic B | 2003 | IEEE Transactions on Aerospace and Electronic systems2003，39，3： | 1 |
| 17 | Symptomatic presentationwith cervical cancer in Uganda: a qualitative study assessing thepathways to diagnosis in a low-income country 显示文摘 | Mwaka AD Okello ES Wabinga H | 2015 | BMC WomensHealth2015，15，1： | 1 |
| 18 | Maximum likelihood registration of dissimilar sensors显示文摘 | McMichael D Okello N | 2003 | IEEE Trans on Aerospace and Electronics Systems2003，39，3： | 1 |
| 19 | Maximum Likelihood Registration for Multiple Dissimilar Sensors显示文摘 | OKELLO Nickens RISTIC Branko | 2003 | IEEE Transaction on Aerospace and Electronic Systems2003，39，3： | 1 |
| 20 | Comparison of recursive algorithms for emitter localisation using TDOA measurements from a pair of UAVs显示文摘 | Okello N Fletcher F Musicki D | | 0，，03： | 1 |
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