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| 1 | Expression of adhesion molecules on mature cholangiocytes in canal of Hering and bile ductules in wedge biopsy samples of primary biliary cirrhosis显示文摘AIM: To examine the expression of intercellular adhesion molecule-1 (ICAM-1) and lymphocyte function-associated antigen-1 (LFA-1) expression on canals of Hering (CoH)and bile ductules associated with the autoimmune process of bile duct destruction in primary biliary cirrhosis (PBC).METHODS: Ten wedged liver biopsies of PBC (five cases each of stages 2 and 3) were studied. The liver specimens were processed for transmission electron microscopy.Immunohistochemistry was performed using anti-ICAM-1 and anti-LFA-1 mouse mAbs. In situ hybridization was done to examine the messenger RNA expression of ICAM-1 in formalin-fixed, paraffin-embedded sections using peptide nucleic acid probes and the catalyzed signal amplification (CSA) technique. Immunogold-silver staining for electron microscopy was performed using anti-ICAM and anti-LFA-1 mouse mAbs. The immunogold particles on epithelial cells of bile ductules and cholangiocytes of CoH cells were counted and analyzed semi-quantitatively.Western blotting was performed to confirm ICAM-1 protein expression.RESULTS: In liver tissues of PBC patients, immunohistochemistry showed aberrant ICAM-1 expression on the plasma membrane of epithelial cells lining bile ductules,and also on mature cholangiocytes but not on hepatocytes in CoH. LFA-1-positive lymphocytes were closely associated with epithelial cells in bile ductules. ICAM-1 expression at protein level was confirmed by Western blot. In situ hybridization demonstrated ICAM-1 mRNA expression in bile ductules and LFA-1 mRNA in lymphocytes infiltrating the bile ductules. By immunoelectron microscopy, ICAM-1 was demonstrated on the basal surface of epithelial cells in bile ductules and on the luminal surfaces of cholangiocytes in damaged CoH. Cells with intermediate morphology resembling progenitor cells in CoH were not labeled with ICAM-1 and LFA-1.CONCLUSION: De novo expression of ICAM-1 both on mature cholangiocytes in CoH and epithelial cells in bile ductules in PBC implies that lymphocyte-induced destruction through adhesion by ICAM-1 and binding of LFA-1-expressing activated lymphocytes takes place not only in bile ductules but also in the CoH. | Hiroaki Yokomori Masaya Oda Mariko Ogi Go Wakabayashi Shigeyuki Kawachi Kazunori Yoshimura Toshihiro Nagai Masaki Kitajima Masahiko Nomura Toshifumi Hibi | 2005 | World Journal of Gastroenterology2005，11，28： | 4 |
| 2 | 铝合金基石墨复合材料的阻尼行为显示文摘用内耗技术研究了石墨颗粒/铝硅复合材料的低频阻尼行为,考查了阻尼对石墨含量、测量频率、温度和应变振帽的依赖关系以及相应的弹性模量的变化,比较了铸态和挤压变形复合材料的阻尼特征。对其阻尼机制作了初步的探讨。 | 谭启 陈锋 何德坪 Hrofumi Migahara Keiseku Ogi Michiru Sakamoto | 1994 | 复合材料学报1994，11，4： | 3 |
| 3 | The leaf extract of Mallotus japonicus and its major active constituent, rutin,suppressed on melanin production in murine B16F1 melanoma显示文摘Objective: To find anti-melanogenesis materials used in whitening cosmetics.Methods: The ethanolic leaf extract of Mallotus japonicus(M. japonicus) having an anti-melanogenesis activity was separated by a sephadex LH-20 chromatography. Each fraction was measured for its tyrosinase inhibitory activity together with its polyphenol content using the Folin–Ciocalteu method. The anti-melanogenesis activity of the active fractions was determined by the melanin content in the murine B16F1 melanoma. The active fractions were put together due to similar constituents, and then separated by high performance liquid chromatography using a C-18 ODS column. The major antimelanogenesis compound was identified using1 H and13C-NMR and liquid chromatography-mass spectrometry.Results: The ethanolic leaf extract of M. japonicus showed an anti-tyrosinase activity with a high polyphenol content, resulting in suppression of melanin production in the B16F1 melanoma. The extract was separated and the active compound was identical as rutin based on the1 H,13C-NMR and liquid chromatography-mass spectrometry analysis data. In addition, the rutin treatment with cells reduced the melanin content in a concentration dependent manner without any cell toxicity. The leaf extract of M. japonicus containing rutin would be useful in whitening cosmetics for protection from UV-light exposure to be limiting the accumulation of melanin in skin.Conclusions: The leaf extract of M. japonicus and/or rutin isolated from the extract as a key whitening agent would be useful as a whitening cosmetic material for protecting against disorder skin due to melanin accumulation. | Junsei Taira Eito Tsuchida Masatsugu Uehara Natsuko Ohhama Wakana Ohmine Takayuki Ogi | 2015 | Asian Pacific Journal of Tropical Biomedicine2015，5，10： | 2 |
| 4 | Mental effects of an early invention program for very low birth weight infants显示文摘 | Arisawa K Takahashi T | 2001 | No To Hatta2001，33，1： | 1 |
| 5 | Expression of E-cadherin, α-catenin, and β-catenin in the process of lymph node metastasis in oral squamous cell carcinoma 显示文摘 | Tanaka N Odajima T Ogi K | 2003 | Bri J Cancer2003，89，： | 1 |
| 6 | Streptococcus suis: past and present显示文摘 | Staats J Feder I Okwumabua Ogi | 1997 | Vet Res Commun1997，21，： | 1 |
| 7 | Effect of limited jawmotion on ankylosis of the teml-x)ro mandibular joint in sheep显示文摘 | Miyamoto H Kurial K Ogi N | 2000 | Br J O ral Maxillolae Strg2000，38，2： | 1 |
| 8 | Molecular clining and characterization of rat pituitary adenylate cyclase activating polypeptide(PACAP)显示文摘 | | 1990 | Biochem Biophys Res Commun1990，173，6： | 1 |
| 9 | The effect of an intraarticular bone fragment in the genesis of temporomandibular joint ankylosis显示文摘 | Miyamoto H Kurita K Ogi N | 2000 | Int J Oral Maxillofac Surg2000，29，4： | 1 |
| 10 | Biomass energy potential in Thailand 显示文摘 | Yokoyama S Y Ogi T Nalampoon A | 2000 | Biomass and Bioenergy2000，18，5： | 1 |
| 11 | Cloning and characterization of the gene encoding the glutamate dehydrogenase of Streptococcus suis serotype 2 显示文摘 | Ogi Okwumabua Julia S Persaud PG Reddy | 2001 | Clin Diag Lab Immunol2001，8，2： | 1 |
| 12 | Line-focusing electromagnetic acoustic transducers for the detection of slit defects显示文摘 | OGI H HIRAO M OHTANI T | 1999 | IEEE Transactions on Ultrasonics Ferroelectrics and Frequency Control1999，46，2： | 1 |
| 13 | Distribution and localization of caveolin-1 in sinusoidal cells in rat liver显示文摘 | Ogi M Yokomori H Oda M | 2003 | Med Electron Mi- crosc2003，36，1： | 1 |
| 14 | The role of the disk in sheep temporomandibular joint ankylosis显示文摘 | Kurita K Ogi N | 1999 | Oral Surg Oral Med Oral Pathol Oral Radiol Endod1999，88，： | 1 |
| 15 | A sheep model for temporomandibular joint ankylosis显示文摘 | Ishimaru JI Ogi N | 1999 | J Oral Maxillofac Surg1999，57，： | 1 |
| 16 | Risk factor for infection and immunoglobulin replacement therapy in adult nephrotic syndrome显示文摘 | Ogi M Yokoyama H Tomosugi N | 1994 | Am J Kidney Dis1994，24，3： | 1 |
| 17 | Liquefaction of cellulose in hot compressed water using sodium carbonates:products distribution at different reaction temperatures显示文摘 | Minowa T Zhen F Ogi T | 1997 | J Chem Eng Japan1997，30，1： | 1 |
| 18 | Solidification Structres of Fe-C-Cr-(V-Nb-W)alloys显示文摘 | Sa Wamoto A Ogi K Matsda K | 1986 | AFS Tran sactions1986，，94： | 1 |
| 19 | Organic composition of liquidized sewage sludge 显示文摘 | INOUE S SAWAYAMA S OGI T | 1996 | Biomass Bioenergy1996，10，1： | 1 |
| 20 | Expression of heat shock proteins in developing and degenerating rat testes显示文摘 | Ogi S Tanji N Iseda T | 1999 | Arch Androl1999，43，3： | 1 |
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