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| 1 | 在大剂量5-氟尿嘧啶治愈荷瘤小鼠过程中外周血细胞的变化与肿瘤消退之间的相关性分析显示文摘目的:观察在单一剂量5-氟尿嘧啶(5-FU)治愈荷瘤小鼠过程中外周血细胞数量的变化与肿瘤结节缩小之间的关系,为进一步研究化疗治愈肿瘤的作用机制奠定基础。方法:利用单一剂量5-FU治愈荷瘤野生型C57BL/6小鼠模型,荷瘤小鼠腹腔内分别注入25、75、135mg/kg的5-FU,等量生理盐水对照,确定5-FU治愈肿瘤的量效关系。然后,再取6只荷瘤小鼠,分别在75mg/kg5-FU治疗前3d、治疗后第1、4、7、11、15、28天内眦静脉取血,进行血常规检测,分析外周血白细胞、血红蛋白和血小板的变化与荷瘤小鼠肿瘤结节缩小之间的相关性。结果:75、135mg/kg的5-FU均可使荷瘤小鼠的肿瘤结节在治疗后1周内逐渐缩小、直至消退,因此5-FU治愈荷瘤小鼠的最低有效量为75mg/kg。75mg/kg5-FU治疗后第1天外周血白细胞降为(5.0±1.1)×109/L,并持续到化疗后第4天,与治疗前(9.5±1.58)×109/L比较差异有统计学意义(P<0.01);5-FU治疗后第7~15天外周血白细胞的数量升至化疗前水平,随后逐渐下降,在治疗后第28天降至(5.9±1.96)×109/L,低于化疗前水平(P<0.01)。Hb含量在5-FU治疗后第1天也出现降低,由化疗前的(133±7)g/L降为(112±9)g/L,差异有统计学意义(P<0.01),随后Hb含量持续在低水平,治疗后15d逐渐恢复至化疗前水平。75mg/kg的5-FU对荷瘤小鼠血小板的抑制作用不明显,反而在治疗后第1、11天出现2次血小板一过性增高(P<0.01)。结论:75mg/kg5-FU可引起外周血白细胞数和血红蛋白浓度的降低,但对血小板的抑制作用不明显。5-FU发挥抗肿瘤作用使荷瘤小鼠的肿瘤结节缩小与化疗后外周血白细胞和Hb的降低无关,而与化疗后第1天血小板计数的增加有关。 | 李传刚 李墨林 舒晓宏 刘用楫 Michael Notter 秦志海 | 2008 | 细胞与分子免疫学杂志2008，24，2： | 6 |
| 2 | Single nucleotide polymorphisms in candidate genes are significantly associated with resistance to Haemonchus contortus infection in goats显示文摘Background: Haemonchosis is a major economic problem in goat production in humid, tropical and subtropical regions. The disease is caused by an abomasal nematode, Haemonchus contortus, which is highly pathogenic in small ruminants. The aim of this study was to identifying single-nucleotide polymorphisms(SNP) that were associated with fecal egg counts(FEC) and could be used as markers to identify resistance to H. contortus in goats.Results: Ten novel variants in the CI TA, ATP2 A3, HSPA8, STAT5 B, ESYT1, and SERPING1 genes were associated with FEC in goats with a nominal significance level of P < 0.05. Two missense mutation in the exon region of the caprine CI TA gene resulted in replacement of arginine with cysteine at position 9473550(R9473550 C) and aspartic acid with glutamic acid at position 9473870(D9473870 E). Chinese goat breeds had significantly higher FEC than Bangladeshi goat breeds within their respective genotypes. Polymorphism information content(PIC), effective al ele number(Ne), and heterozygosity(He)were greatest for the STAT5 B197A > G SNP locus in al goat breeds. Pairwise coefficients of linkage disequilibrium(D′, r2)revealed complete LD(r2= 1) between significant SNP polymorphisms in CI TA and SERPING1 and strong LD(r2= 0.93 and0.98) between polymorphisms in HSPA8 and ATP2 A3, respectively. Correlation coefficient(r) between FEC and body weight(BW) was significantly positive(r = 0.56***, P < 0.001) but that between FEC and packed cel volume(PCV) was negatively significant(r =-0.47**, P < 0.01) in the total population of goats. On the other hand, correlation coefficient(r)between BW and PCV was not significant in total population of goats. Association analysis revealed that haplotypes within ATP2 A3, HSPA8, and SERPING1 were significantly associated with FEC. Quantitative real-time PCR revealed that the relative expression of m RNA was higher(P < 0.001) for resistant, compared to susceptible, groups of goats for al candidate genes except CI TA.Conclusions: This study identified SNP markers that can potential y be used in marker-assisted selection programs to develop goat breeds that are resistant to H. contortus. | Mahmuda Bilkis Bintee Alam Abdullah Ibne Omar Md.Omar Faruque David Russell Notter Kathiravan Periasamy Md.Motahar Hussain Mondal Md.Jalal Uddin Sarder Md.Shamsuddin Jianhua Cao Xiaoyong Du Zhenyang Wu Shuhong Zhao | 2019 | Journal of Animal Science and Biotechnology2019，10，2： | 2 |
| 3 | γ-干扰素在小剂量美法仑治愈肿瘤过程中对荷瘤小鼠外周血血小板计数的影响及其意义显示文摘目的:探讨γ-干扰素(IFN-γ)在小剂量美法仑治愈肿瘤过程中对荷瘤小鼠外周血血小板(PLT)计数的影响及其意义。方法:利用单一剂量美法仑治愈荷瘤野生型C57BL/6小鼠模型,取遗传背景相同、基因型不同的IFN-γ+/-和IFN-γ-/-C57BL/6小鼠各6只,观察7.5 mg/kg美法仑治疗后两组荷瘤小鼠肿瘤结节直径的变化,并且分别在美法仑用药前第3天、用药后6 h、用药后第4、7、11、15、28天内眦静脉取血,肝素抗凝后进行血常规检测,观察两组荷瘤小鼠外周血PLT计数的动态变化并进行比较,了解美法仑治疗后荷瘤小鼠化疗的疗效及其外周血PLT计数的变化与体内IFN-γ免疫功能之间的关系。结果:美法仑用药前两组荷瘤小鼠肿瘤结节的直径和外周血PLT计数比较差异无统计学意义(P>0.05)。7.5 mg/kg美法仑用药后第1天,两组荷瘤小鼠肿瘤结节缩小均不明显;用药后第4天,荷瘤IFN-γ-/-小鼠肿瘤生长轻度受抑后进行性增大,并在用药后2周内所有小鼠肿瘤复发、死亡,而对照组IFN-γ+/-小鼠肿瘤的肿瘤结节进行性缩小、肿瘤消退并治愈。7.5 mg/kg美法仑用药后6 h,荷瘤IFN-γ+/-小鼠外周血PLT计数较前明显升高,达(1935±378)×109/L(P<0.01),而荷瘤IFN-γ-/-小鼠外周血PLT计数较前无明显升高,为(1183±186)×109/L(P>0.05),两组数据比较差异具有统计学意义(P<0.01)。美法仑用药1 d以后,荷瘤IFN-γ+/-小鼠外周血PLT计数较前逐渐下降,用药后第11天降至(1158±270)×109/L,恢复至化疗前水平(P>0.05);而荷瘤IFN-γ-/-小鼠外周血PLT计数一直维持在正常水平;两组荷瘤小鼠外周血PLT计数比较差异无统计学意义。结论:小剂量美法仑治疗荷瘤C57BL/6小鼠过程中,IFN-γ免疫功能正常的荷瘤IFN-γ+/-小鼠在用药后6 h出现外周血血小板计数的'一过性'增高,小鼠肿瘤治愈;而IFN-γ免疫功能缺陷的荷瘤IFN-γ-/-小鼠在用药后6 h不出现外周血PLT计数的增加,小鼠肿瘤复发死亡。提示在小剂量美法仑治愈肿瘤过程中荷瘤小鼠外周血PLT计数的升高与其体内IFN-γ的免疫功能密切相关。 | 李传刚 舒晓宏 韩丹 李墨林 Michael Notter 秦志海 | 2009 | 细胞与分子免疫学杂志2009，25，11： | 2 |
| 4 | 小剂量美法仑治愈荷瘤小鼠过程中外周血细胞计数的变化及其意义显示文摘目的:观察在小剂量美法仑治愈荷瘤C57BL/6小鼠过程中外周血细胞计数的变化与肿瘤结节缩小之间的关系,为进一步研究化疗治愈肿瘤的作用机制奠定基础。方法:野生型C57BL/6小鼠皮下接种小鼠淋巴瘤EL4细胞,建立荷EL4肿瘤的小鼠模型。肿瘤接种后12d,12只荷瘤小鼠随机分成2组,7.5mg/kg美法仑单次腹腔内注射,等量生理盐水对照。观察荷瘤小鼠肿瘤结节直径的变化。用药组小鼠分别在7.5mg/kg美法仑治疗前3d、治疗后6h、治疗后第4、7、11、15、28天内眦静脉取血,进行血常规检测,分析荷瘤小鼠外周血象与荷瘤小鼠肿瘤结节缩小之间的相关性。结果:7.5mg/kg美法仑治疗后,荷瘤小鼠肿瘤消退;而对照组荷瘤小鼠肿瘤继续生长。美法仑治疗后6h外周血白细胞为(10.6±2.3)×109/L,与治疗前(9.8±0.32)×109/L比较差异无统计学意义(P>0.05);治疗后第4天外周血白细胞降为(2.9±0.97)×109/L,与治疗前比较差异具统计学意义(P<0.01),随后尽管白细胞数有所回升,但在治疗后第28天仍明显低于化疗前水平(P<0.01)。Hb含量在美法仑治疗后6h由化疗前的(132±7)g/L降为(110±14)g/L(P<0.05),随后Hb含量持续降低,治疗后第7天达最低点(96±5)g/L,以后逐渐升高,治疗后15d恢复至化疗前水平。用药后6h荷瘤小鼠血小板计数升至(1502±142)×109/L,与用药前(914±322)×109/L比较差异具有统计学意义(P<0.01),随后1周内一直维持在高水平,治疗后第11天血小板恢复至化疗前水平。结论:美法仑在使荷瘤小鼠的肿瘤结节缩小的同时可引起外周血白细胞数和血红蛋白浓度的降低,但对血小板无抑制作用。美法仑化疗后1周内血小板计数的增加可能与美法仑的抗肿瘤作用有关。 | 舒晓宏 李传刚 李墨林 Michael Notter 秦志海 | 2009 | 细胞与分子免疫学杂志2009，25，6： | 2 |
| 5 | Characterization of neurotransmitter phenotype during neuronal differentiation of embryonal carcinoma cells显示文摘 | Sharma S Notter MF | 1988 | Dev Biol1988，125，2： | 1 |
| 6 | Lung surfactant replacement in premature lambs with extracted lipids from bovine lung lavage:effects of dose,dispersion technique,and gestational age显示文摘 | Notter RH Egan EA Kwong MS | | 0，，6： | 1 |
| 7 | Component-specific surface and physiological activity in bovine-derived lung surfactants 显示文摘 | Notter RH Wang Z Egan EA | 2002 | Chem Phys Lipids2002，114，1： | 1 |
| 8 | Surfactant dysfunction in oleic acid injured lungs显示文摘 | Hall S B Notter R H Smith R J | 1988 | Am Rev Respir Dis1988，137，： | 1 |
| 9 | Effects of the lung sur- factant protein B construct Mini - B on lipid bilayer order and topography显示文摘 | Palleboina D Waring A J Notter RH | 2012 | Eur Biophys2012，41，9： | 1 |
| 10 | Porosity of electrodeposited coatings: its cause, nature, effect and management 显示文摘 | NOTTER I M GABE D R | 1992 | Corrosion Reviews1992，10，34： | 1 |
| 11 | Genetic improvement of reproductive efficiency of sheep and goats显示文摘 | Notter D R | 2012 | Anim Reprod Sci2012，130，34： | 1 |
| 12 | Genetic improvement of reproductive efficiency of sheep and goats显示文摘 | Notter D R | 2012 | Anim Reprod Sci2012，130，34： | 1 |
| 13 | Isolation of type Ⅱ pneumocytes by laser flow cytometry显示文摘 | Leary JF Finkelstein JN Notter RH | 1982 | Am Rev Respir Dis1982，125，3： | 1 |
| 14 | Surfactant treatment of full-term neoborns with severe respiratory failure 显示文摘 | Auten RL Notter RH Kendig JW | 1991 | Pediatrics1991，87，： | 1 |
| 15 | Thermal expansion models for polycrystalline salt-ceramics显示文摘 | NOTTER W HAHNE E | 1996 | Thermochimica Acta1996，290，： | 1 |
| 16 | Thermophysical properties of the composite ceramic-salt system显示文摘 | NOTTER W LECHNER T HAHNE E | 1993 | Thermochimica Acta1993，218，： | 1 |
| 17 | Relationship between milk expected progeny differences of polled Hereford sires and actual milk production of their crossbred daughters显示文摘 | Notter D R Beal W E | 1992 | J Anim Sci1992，70，： | 1 |
| 18 | Preparing for loop ileostomy surgery:womens accounts from a qualitative study显示文摘 | Notter J Burnard P | 2006 | Int J Nurs Stud2006，43，： | 1 |
| 19 | Polarisation resistance methods for measurement of the porosity of thin metal coatings显示文摘 | Notter I M Gabe D R | 1993 | Corrosion Science1993，34，5： | 1 |
| 20 | Body measurements of newborn calves and relationship of calf shape to sire breeding values for birth weight and calving ease显示文摘 | Notter III D R Beal W E | 1991 | J Anim Sci1991，69，： | 1 |
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