|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | 基于高压制备液相的多维色谱技术在中药分离纯化中的应用显示文摘中药物质基础复杂,对其活性成分的分离一直是中药研究的难题。基于高压制备液相的多维色谱系统在高压制备液相色谱的基础上,结合了多种分离技术,极大地提高了色谱系统的分离性能和分离效率,更有利于对物质基础复杂的中药样品进行分离纯化。本文介绍了基于高压制备液相系统的多维色谱系统的基本原理、分离模式以及关键技术,并综述了其在中药分离纯化中的应用。 | 谢秀满 孙万阳 黄竞怡 Navaneethakrishnan Polachi 佟玲 孙国祥 | 2016 | 分析化学2016，44，7： | 12 |
| 2 | 注射用益气复脉(冻干)在正常和慢性心衰大鼠体内的药动学研究显示文摘目的研究注射用益气复脉(冻干,YQFM)中10种人参皂苷成分(Rg1、Rb1、Rc、Rd、Re、Rf、Rg3、Rh1、Rb2和Rb3)在正常和慢性心力衰竭模型大鼠体内的药动学规律与差异,为其临床合理用药提供参考。方法 12只Wistar大鼠随机分为对照组和模型组,每组6只。采用冠状动脉结扎法建立慢性心衰大鼠模型,造模14 d后,两组大鼠均iv给予YQFM543 mg/kg。建立超高效液相色谱-质谱联用(UFLC-MS/MS)法,测定不同时间点各人参皂苷的血药浓度,绘制药时曲线,采用DAS 3.0药动学软件计算药动学参数,并进行比较。结果 iv YQFM后,各皂苷成分在对照组和慢性心力衰竭模型组大鼠体内的主要药动学参数T_(1/2)、AUC_(0-t)、AUC_(0-∞)、CL等,均没有显著性差异。结论在慢性心力衰竭状态下,机体对YQFM中皂苷成分的代谢无明显影响。 | 赵利斌 王辉 李伟 褚扬 张文静 Navaneethakrishnan Polachi 李德坤 周大铮 鞠爱春 刘昌孝 | 2018 | 药物评价研究2018，41，3： | 1 |
| 3 | 注射用丹参多酚酸对阿司匹林在大鼠体内的药动学与药效学影响显示文摘目的:研究注射用丹参多酚酸对阿司匹林在大鼠体内的药动学和药效学影响。方法:药动学方面,12只Wistar大鼠随机分为2组(阿司匹林组、注射用丹参多酚酸与阿司匹林联合用药组),采用建立的LC-MS/MS法,测定不同时间大鼠血浆中阿司匹林活性代谢产物水杨酸的浓度。药效学方面,24只Wistar大鼠随机分为4组(正常对照组、阿司匹林组、注射用丹参多酚酸组、联合用药组)采血测定凝血时间、血小板聚集率,采用剪尾法测定出血时间。结果:与单用阿司匹林组相比,联合用药组水杨酸C_(max),T_(max),t_(1/2),AUC_(0~t),AUC_(0~∞)以及凝血、出血时间均未有显著性差异,血小板抑制率有明显的增高(P<0.05)。结论:注射用丹参多酚酸不会对阿司匹林在大鼠体内的代谢造成显著影响,二者联用能有效增强血小板抑制率,但不会造成出血风险。 | 张文静 褚扬 刘万卉 李德坤 周大铮 陈丽萍 Navaneethakrishnan Polachi 李伟 鞠爱春 | 2018 | 中国新药杂志2018，27，7： | 1 |
| 4 | Isocoreopsin: An active constituent of n-butanol extract of Butea monosperma flowers against colorectal cancer(CRC)显示文摘The herb Butea monosperma constitutes several human health beneficial components, which are mostly studied for their anticancer effects. In this study, the activity of n-butanol fractions of B. monosperma floral extract was examined on inhibiting aberrant crypt foci(ACF) formation in azoxymethane induced Wistar albino rats. The n-butanol extracts(150 mg/kg) decreased the ACF formation(per rat) by 92% and78% in short- and long-term in vivo treatments, respectively. All the compounds in the n-butanol extract were isolated and purified using column and reverse-phase high pressure liquid chromatography(HPLC).Their structures were characterized using UV–visible spectroscopy, nuclear magnetic resonance(NMR)and electrospray–ionisation mass spectrometry(ESI–MS) to determine important flavonoids, namely isocoreopsin, butrin and isobutrin. These compounds were studied for their free radical scavenging and anticancer activities. The compound isocoreopsin showed significantly greater efficacy in cell death on human colon and liver cancer cell lines(50 μg/m L in HT-29 and 100 μg/m L in Hep G2) than butrin(100 μg/m L in HT-29 and 500 μg/m L in Hep G2) and isobutrin(80 μg/m L in HT-29 and 150 μg/m L in Hep G2). These results suggest that isocoreopsin, butrin and isobutrin are the important key compounds for the chemoprevention of colon cancer and isocoreopsin can be considered as a promising novel drug. | Boopathi Subramaniyan Navaneethakrishnan Polachi Ganeshan Mathan | 2016 | Journal of Pharmaceutical Analysis2016，6，5： | 0 |
您所在的机构暂未开通此部分的功能，请联系管理员