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| 1 | Engineering osteoarthritic cartilage model through differentiating senescent human mesenchymal stem cells for testing disease-modifying drugs显示文摘Significant cellular senescence has been observed in cartilage harvested from patients with osteoarthritis(OA).In this study,we aim to develop a senescence-relevant OA-like cartilage model for developing disease-modifying OA drugs(DMOADs).Spe-cifically,human bone marrow-derived mesenchymal stromal cells(MSCs)were expanded in vitro up to passage 10(P10-MSCs).Following their senescent phenotype formation,P10-MSCs were subjected to pellet culture in chondrogenic medium.Results from qRT-PCR,histology,and immunostaining indicated that cartilage generated from P10-MSCs displayed both senescent and OA-like phenotypes without using other OA-inducing agents,when compared to that from normal passage 4(P4)-MSCs.Interestingly,the same gene expression differences observed between P4-MSCs and P10-MSC-derived cartilage tissues were also observed between the preserved and damaged OA cartilage regions taken from human samples,as demonstrated by RNA sequencing data and other analysis methods.Lastly,the utility of this senescence-initiated OA-like cartilage model in drug development was assessed by testing several potential DMOADs and senolytics.The results suggest that pre-existing cellular senescence can induce the generation of OA-like changes in cartilage.The P4-and P10-MSCs derived cartilage models also represent a novel platform for predicting the efficacy and toxicity of potential DMOADs on both preserved and damaged cartilage in humans. | Ning Wang Yuchen He Silvia Liu Meagan J.Makarcyzk Guanghua Lei Alexander Chang Peter G Alexander Tingjun Hao Anne-Marie Padget Nuria de Pedro Tsapekos Menelaos Hang Lin | 2022 | Science China(Life Sciences)2022，65，2： | 5 |
| 2 | Tumor necrosis factor-α-induced protein 1 and immunity to hepatitis B virus显示文摘AIM: To compare the gene expression profile in a pair of HBV-infected twins.METHODS: The gene expression profile was compared in a pair of HBV-infected twins.RESULTS: The twins displayed different disease outcomes. One acquired natural immunity against HBV,whereas the other became a chronic HBV carrier. Eightyeight and forty-six genes were found to be up- or downregulated in their PBMCs, respectively. Tumor necrosis factor-alpha-induced protein 1 (TNF-αIP1) that expressed at a higher level in the HBV-immune twins was identified and four pairs of siblings with HBV immunity by RTPCR. However, upon HBV core antigen stimulation,TNF-αIP1 was downregulated in PBMCs from subjects with immunity, whereas it was slightly upregulated in HBV carriers. Bioinformatics analysis revealed a K+channel tetramerization domain in TNF-αIP1 that shares a significant homology with some human, mouse, and C elegan proteins.CONCLUSION: TNF-αIP1 may play a role in the innate immunity against HBV. | Marie C Lin Nikki P Lee Ning Zheng Pai-Hao Yang Oscar G Wong Hsiang-Fu Kung Chee-Kin Hui John M Luk George Ka-Kit Lau | 2005 | World Journal of Gastroenterology2005，11，48： | 3 |
| 3 | Mecobalamin显示文摘 | Zhang YF Ning G | 2008 | Expert Opin Investig Drugs2008，17，6： | 1 |
| 4 | Relationship of urinary bisphenol Aconcentration to risk for prevalent type-2 diabetes in Chinese adults显示文摘 | Ning G Bi Y Wang T | 2011 | Annal Internal Med2011，155，6： | 1 |
| 5 | Bone marrow cells stained by azide-conjugated Alexa fluors in the absence of an alkyne label 显示文摘 | Lin G Ning H Banie L | 2012 | Stem Cells Dev2012，21，： | 1 |
| 6 | Neuron-like differentiation of adipose tissue-derived stromal cells and vascular smooth muscle cells显示文摘 | Ning H Lin G Lue TF | 2006 | Differentiation2006，74，9： | 1 |
| 7 | Aircraft route op- timization for formation flight 显示文摘 | Xu J S Ning S A Bower G C Kroo I M | 2014 | Journal of Aircraft2014，51，2： | 1 |
| 8 | A new and practical application of JH antagonist KK-42 to promoting growth of shrimp Peaaeus schrnitti显示文摘 | Ning Q J Fu S G Xu X J He J T | 2007 | Aquaculture2007，270，1： | 1 |
| 9 | Thermoplastic sandwich structure design and manufacturing for the body panel of mass transit vehicle 显示文摘 | NING H B JANOWSKI G M | 2007 | Compos Struct2007，80，： | 1 |
| 10 | The performances of BMI nanocomposites filled with nanometer SIC显示文摘 | Yan H X Ning R C Liang G Z Ma X Y | 2005 | Journal of Applied Polymer Science2005，95，5： | 1 |
| 11 | Porous and lamella-like Fe/MgO catalysts prepared under hydrothermal conditions for high-yield syn thesis of double-walled carbon nanotubes显示文摘 | Ning G Q Liu Y Wei F | 2007 | J Phys Chem C2007，111，5： | 1 |
| 12 | Leptin inhibits apolipoprotein M transcription and secretion in human hepatoma cell line,HepG2 cells显示文摘 | Luo G Hurtig M Zhang X Peter Nilsson-Ehle Ning XU | 2005 | Biochim Biophys Acta2005，1734，2： | 1 |
| 13 | Storytellingresearch of consumers’self-reports of urban tourism experiencesin China显示文摘 | Hsu Shih-Yun Dehuang Ning Woodside Arch G | 2009 | Journal of Business Research2009，62，12： | 1 |
| 14 | Sixty-four-slice mul- tideteetor computed tomography for preoperative evaluation of left ventricular function and mass in patients with mitral re- gurgitation: comparison with magnetic resonance imaging and echocardiography显示文摘 | GUO Y K YANG Z G NING G | 2009 | Eur Radiol2009，19，9： | 1 |
| 15 | Relationship of urinary bisphenol A concentration to risk for prevalent type-2 diabetes in Chinese adults显示文摘 | Ning G Bi Y Wang T | 2011 | Annal Internal Med2011，155，6： | 1 |
| 16 | Treatment of three- and four-part proximal humeral fractures with locking proximal humerus plate显示文摘 | Sun J C Li Y L Ning G Z Wu Q Feng S Q | 2013 | Eur J Orthop Surg Traumatol2013，23，： | 1 |
| 17 | Particle-epithelial interaction:effect ofpriming and bystander neutrophils on interleuin-8 release显示文摘 | Ning Y Tao F Qin G | 2004 | Am JRespir Cell Mol Biol2004，30，： | 1 |
| 18 | Stochastic resonance without external periodic force显示文摘 | Hu G Ditzinger T Ning C Z | 1993 | Phys Rev Lett1993，71，： | 1 |
| 19 | A novel opinion about the coupling loss between single-mode fibers and waveguides显示文摘 | FAN G F NING J P SHANG L J | 2006 | J of Optoeleetronies and Advanced Materials2006，8，5： | 1 |
| 20 | Combination of activated Schwann cells with bone mesenchymal stem cells: the best cell strate- gy for repair after spinal cord injury in rats显示文摘 | Ban D X Ning G Z Feng S Q | 2011 | Regen Med2011，6，6： | 1 |
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